Description

Strain Information

Former Names C57BL/6-Tg(ACTB-OVA)916Jen/J    (Changed: 08-MAY-08 ) C57BL/6-Tg(Actb-OVA)916Jen/J    (Changed: 29-MAR-05 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Hemizygote x +/+ sibling         (Female x Male) Species laboratory mouse Generation N?+3 (05-DEC-07)   Donating Investigator Marc Jenkins,   University of Minnesota

Description
Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-K^b complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-A^b complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Development
A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2K^b and the transmembrane region of the H-2D^b gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of ACTB

005703	B6.Cg-Tg(ACTFLPe)9205Dym/J
006055	B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
003800	B6;SJL-Tg(ACTFLPe)9205Dym/J
005863	C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J
002981	DBA/2-Tg(xstpx-lacZ)36And/J
003376	FVB/N-Tg(ACTB-cre)2Mrt/J
008200	FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
005438	STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J

View Strains carrying other alleles of ACTB     (8 strains)

Strains carrying other alleles of OVA

005432	C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ
005433	C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
005431	C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ

View Strains carrying other alleles of OVA     (3 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(CAG-OVA)916Jen/?

        C57BL/6-Tg(CAG-OVA)916Jen

• cellular phenotype
• abnormal cell content/ morphology (MGI Ref ID J:91742)
  • most cells of the body express a membrane bound form of chicken ovalbumin on their cell surface
• immune system phenotype
• abnormal dendritic cell antigen presentation (MGI Ref ID J:91742)
  • dendritic cells in the skin present an epitope of chicken ovalbumin in complex with the MHC allele K^b
  • splenocytes are able to activate OT-I and OT-II transgenic T cells when cultured in vitro
• decreased length of allograft survival (MGI Ref ID J:91742)
  • full thickness tail skin grafted from transgenic mice to C57BL/6 mice have a mean survival time of 33.9 days compared a mean survival time of over 200 days for non-transgenic donors
  • recipient mice show accelerated rejection (18 days) when grafted with transgenic tail skin a second time
  • recipient mice also show accelerated rejection when adoptively transferred with OT-I or OT-II T cells (OT-I = Tg(TcraTcrb)1100Mjb, OT-II = Tg(TcraTcrb)425Cbn)
  • when recipient mice lack T cells (Tcra^tm1Mom homozygotes), rejection of the transgenic skin grafts fail to occur
  • when recipient mice lack CD8 T cells (Cd8a^tm1Mak homozygotes), transgenic skin grafts shrink rapidly to 10-30% of their original size but then survive indefinitely
  • when recipient mice lack CD4 T cells (H2-Ab1^tm1Gru homozygotes), transgenic skin grafts remain healthy for the first 40 days after which they start progressively contracting with 25% of the grafts being lost by 115 days

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research (Tissue/Cell Markers: multiple)
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(CAG-OVA)916Jen
Allele Name		transgene insertion 916, Marc K Jenkins
Allele Type		Transgenic (random, expressed)
Common Name(s)		Act-mOVA; Tg(ACTB-OVA)916Jen; Tg(CAG-OVA)916Jen;
Mutation Made By		Ben Ehst,   University of Minnesota
Strain of Origin		C57BL/6
Expressed Gene		OVA, ovalbumin, chicken
Promoter		ACTB, actin, beta, human
Molecular Note		The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-Kb gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-Db gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. [MGI Ref ID J:91742]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAG-OVA)916Jen, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ehst BD; Ingulli E; Jenkins MK. 2003. Development of a novel transgenic mouse for the study of interactions between CD4 and CD8 T cells during graft rejection. Am J Transplant 3(11):1355-62. [PubMed: 14525595]  [MGI Ref ID J:91742]

Additional References

Tg(CAG-OVA)916Jen related

Janssen EM; Droin NM; Lemmens EE; Pinkoski MJ; Bensinger SJ; Ehst BD; Griffith TS; Green DR; Schoenberger SP. 2005. CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death. Nature 434(7029):88-93. [PubMed: 15744305]  [MGI Ref ID J:96586]

Kang J; Huddleston SJ; Fraser JM; Khoruts A. 2008. De novo induction of antigen-specific CD4+CD25+Foxp3+ regulatory T cells in vivo following systemic antigen administration accompanied by blockade of mTOR. J Leukoc Biol 83(5):1230-9. [PubMed: 18270248]  [MGI Ref ID J:134458]

Mayerova D; Parke EA; Bursch LS; Odumade OA; Hogquist KA. 2004. Langerhans cells activate naive self-antigen-specific CD8 T cells in the steady state. Immunity 21(3):391-400. [PubMed: 15357950]  [MGI Ref ID J:93786]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	As preliminary attempts to make a homozygous colony at The Jackson Laboratory yielded smaller than normal homozygous males (September 2006), this strain is maintained by breeding hemizygotes to wildtype siblings or C57BL/6J mice.
Mating System	Hemizygote x +/+ sibling         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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