Strain Name:

C57BL/6-Tg(CAG-OVA)916Jen/J

Stock Number:

005145

Availability:

Repository- Live

Description

Strain Information

Former Names C57BL/6-Tg(ACTB-OVA)916Jen/J    (Changed: 08-MAY-08 )
C57BL/6-Tg(Actb-OVA)916Jen/J    (Changed: 29-MAR-05 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHemizygote x +/+ sibling         (Female x Male)
Specieslaboratory mouse
GenerationN?+3 (05-DEC-07)
 
Donating Investigator Marc Jenkins,   University of Minnesota

Description
Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-Kb complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-Ab complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Development
A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2Kb and the transmembrane region of the H-2Db gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of ACTB     (8 strains)

Strains carrying other alleles of OVA
005432   C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ
005433   C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
005431   C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
View Strains carrying other alleles of OVA     (3 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(CAG-OVA)916Jen/?

        C57BL/6-Tg(CAG-OVA)916Jen
  • cellular phenotype
  • abnormal cell content/ morphology (MGI Ref ID J:91742)
    • most cells of the body express a membrane bound form of chicken ovalbumin on their cell surface
  • immune system phenotype
  • abnormal dendritic cell antigen presentation (MGI Ref ID J:91742)
    • dendritic cells in the skin present an epitope of chicken ovalbumin in complex with the MHC allele Kb
    • splenocytes are able to activate OT-I and OT-II transgenic T cells when cultured in vitro
  • decreased length of allograft survival (MGI Ref ID J:91742)
    • full thickness tail skin grafted from transgenic mice to C57BL/6 mice have a mean survival time of 33.9 days compared a mean survival time of over 200 days for non-transgenic donors
    • recipient mice show accelerated rejection (18 days) when grafted with transgenic tail skin a second time
    • recipient mice also show accelerated rejection when adoptively transferred with OT-I or OT-II T cells (OT-I = Tg(TcraTcrb)1100Mjb, OT-II = Tg(TcraTcrb)425Cbn)
    • when recipient mice lack T cells (Tcratm1Mom homozygotes), rejection of the transgenic skin grafts fail to occur
    • when recipient mice lack CD8 T cells (Cd8atm1Mak homozygotes), transgenic skin grafts shrink rapidly to 10-30% of their original size but then survive indefinitely
    • when recipient mice lack CD4 T cells (H2-Ab1tm1Gru homozygotes), transgenic skin grafts remain healthy for the first 40 days after which they start progressively contracting with 25% of the grafts being lost by 115 days
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research (Tissue/Cell Markers: multiple)
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(CAG-OVA)916Jen
Allele Name transgene insertion 916, Marc K Jenkins
Allele Type Transgenic (random, expressed)
Common Name(s) Act-mOVA; Tg(ACTB-OVA)916Jen; Tg(CAG-OVA)916Jen;
Mutation Made By Ben Ehst,   University of Minnesota
Strain of OriginC57BL/6
Expressed Gene OVA, ovalbumin, chicken
Promoter ACTB, actin, beta, human
Molecular Note The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-Kb gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-Db gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. [MGI Ref ID J:91742]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAG-OVA)916Jen, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ehst BD; Ingulli E; Jenkins MK. 2003. Development of a novel transgenic mouse for the study of interactions between CD4 and CD8 T cells during graft rejection. Am J Transplant 3(11):1355-62. [PubMed: 14525595]  [MGI Ref ID J:91742]

Additional References

Tg(CAG-OVA)916Jen related

Janssen EM; Droin NM; Lemmens EE; Pinkoski MJ; Bensinger SJ; Ehst BD; Griffith TS; Green DR; Schoenberger SP. 2005. CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death. Nature 434(7029):88-93. [PubMed: 15744305]  [MGI Ref ID J:96586]

Kang J; Huddleston SJ; Fraser JM; Khoruts A. 2008. De novo induction of antigen-specific CD4+CD25+Foxp3+ regulatory T cells in vivo following systemic antigen administration accompanied by blockade of mTOR. J Leukoc Biol 83(5):1230-9. [PubMed: 18270248]  [MGI Ref ID J:134458]

Mayerova D; Parke EA; Bursch LS; Odumade OA; Hogquist KA. 2004. Langerhans cells activate naive self-antigen-specific CD8 T cells in the steady state. Immunity 21(3):391-400. [PubMed: 15357950]  [MGI Ref ID J:93786]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryAs preliminary attempts to make a homozygous colony at The Jackson Laboratory yielded smaller than normal homozygous males (September 2006), this strain is maintained by breeding hemizygotes to wildtype siblings or C57BL/6J mice.
Mating SystemHemizygote x +/+ sibling         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $122.80Female or MaleHemizygous for Tg(CAG-OVA)916Jen
Pairs /Price*Pair Genotype
$175.05Hemizygous for Tg(CAG-OVA)916Jen x Noncarrier
$175.05Noncarrier x Hemizygous for Tg(CAG-OVA)916Jen
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $159.70Female or MaleHemizygous for Tg(CAG-OVA)916Jen
Pairs /Price*Pair Genotype
$227.60Hemizygous for Tg(CAG-OVA)916Jen x Noncarrier
$227.60Noncarrier x Hemizygous for Tg(CAG-OVA)916Jen
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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