Description

Strain Information

Former Names C57BL/6-Tg(ACTB-OVA)916Jen/J    (Changed: 08-MAY-08 ) C57BL/6-Tg(Actb-OVA)916Jen/J    (Changed: 29-MAR-05 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x +/+ sibling         (Female x Male)   07-JUN-08 Species laboratory mouse Generation N?+N14 (27-DEC-12) Generation Definitions   Donating Investigator Marc Jenkins,   University of Minnesota

Description
Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-K^b complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-A^b complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Development
A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2K^b and the transmembrane region of the H-2D^b gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of ACTB

010939	B6.Cg-Tg(ACTB-UPF1*R844C)581Hcd/J
005703	B6.Cg-Tg(ACTFLPe)9205Dym/J
009686	B6.Cg-Tg(Actb-TNFRSF6B)754Jwu/J
008226	B6.FVB-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
003800	B6;SJL-Tg(ACTFLPe)9205Dym/J
005863	C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J
002981	DBA/2-Tg(xstpx-lacZ)36And/J
003376	FVB/N-Tg(ACTB-cre)2Mrt/J
008200	FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
013749	STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
013751	STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017530	STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
005438	STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J

View Strains carrying other alleles of ACTB     (13 strains)

Strains carrying other alleles of OVA

005432	C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ
005433	C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
005431	C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ

View Strains carrying other alleles of OVA     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tg(CAG-OVA)916Jen/?

        C57BL/6-Tg(CAG-OVA)916Jen

• cellular phenotype
• abnormal cell morphology
  • most cells of the body express a membrane bound form of chicken ovalbumin on their cell surface   (MGI Ref ID J:91742)
• immune system phenotype
• abnormal dendritic cell antigen presentation
  • dendritic cells in the skin present an epitope of chicken ovalbumin in complex with the MHC allele K^b   (MGI Ref ID J:91742)
  • splenocytes are able to activate OT-I and OT-II transgenic T cells when cultured in vitro   (MGI Ref ID J:91742)
• decreased length of allograft survival
  • full thickness tail skin grafted from transgenic mice to C57BL/6 mice have a mean survival time of 33.9 days compared a mean survival time of over 200 days for non-transgenic donors   (MGI Ref ID J:91742)
  • recipient mice show accelerated rejection (18 days) when grafted with transgenic tail skin a second time   (MGI Ref ID J:91742)
  • recipient mice also show accelerated rejection when adoptively transferred with OT-I or OT-II T cells (OT-I = Tg(TcraTcrb)1100Mjb, OT-II = Tg(TcraTcrb)425Cbn)   (MGI Ref ID J:91742)
  • when recipient mice lack T cells (Tcra^tm1Mom homozygotes), rejection of the transgenic skin grafts fail to occur   (MGI Ref ID J:91742)
  • when recipient mice lack CD8 T cells (Cd8a^tm1Mak homozygotes), transgenic skin grafts shrink rapidly to 10-30% of their original size but then survive indefinitely   (MGI Ref ID J:91742)
  • when recipient mice lack CD4 T cells (H2-Ab1^tm1Gru homozygotes), transgenic skin grafts remain healthy for the first 40 days after which they start progressively contracting with 25% of the grafts being lost by 115 days   (MGI Ref ID J:91742)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: multiple
Immunology and Inflammation Research
      T Cell Receptor Transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(CAG-OVA)916Jen
Allele Name		transgene insertion 916, Marc K Jenkins
Allele Type		Transgenic (random, expressed)
Common Name(s)		Act-mOVA; ActmOVA; Tg(ACTB-OVA)916Jen; Tg(CAG-OVA)916Jen; actOVA;
Mutation Made By		Ben Ehst,   University of Minnesota
Strain of Origin		C57BL/6
Expressed Gene		OVA, ovalbumin, chicken
Promoter		ACTB, actin, beta, human
Molecular Note		The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-Kb gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-Db gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. [MGI Ref ID J:91742]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAG-OVA)916Jen-alternate1, Standard PCR
Tg(CAG-OVA)916Jen-alternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ehst BD; Ingulli E; Jenkins MK. 2003. Development of a novel transgenic mouse for the study of interactions between CD4 and CD8 T cells during graft rejection. Am J Transplant 3(11):1355-62. [PubMed: 14525595]  [MGI Ref ID J:91742]

Additional References

Tg(CAG-OVA)916Jen related

Baban B; Chandler PR; Sharma MD; Pihkala J; Koni PA; Munn DH; Mellor AL. 2009. IDO activates regulatory T cells and blocks their conversion into Th17-like T cells. J Immunol 183(4):2475-83. [PubMed: 19635913]  [MGI Ref ID J:151475]

Barnes MJ; Aksoylar H; Krebs P; Bourdeau T; Arnold CN; Xia Y; Khovananth K; Engel I; Sovath S; Lampe K; Laws E; Saunders A; Butcher GW; Kronenberg M; Steinbrecher K; Hildeman D; Grimes HL; Beutler B; Hoebe K. 2010. Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice. J Immunol 184(7):3743-54. [PubMed: 20190135]  [MGI Ref ID J:160090]

Coleman MA; Bridge JA; Lane SW; Dixon CM; Hill GR; Wells JW; Thomas R; Steptoe RJ. 2013. Tolerance induction with gene-modified stem cells and immune-preserving conditioning in primed mice: restricting antigen to differentiated antigen-presenting cells permits efficacy. Blood 121(6):1049-58. [PubMed: 23233664]  [MGI Ref ID J:194614]

Desch AN; Randolph GJ; Murphy K; Gautier EL; Kedl RM; Lahoud MH; Caminschi I; Shortman K; Henson PM; Jakubzick CV. 2011. CD103+ pulmonary dendritic cells preferentially acquire and present apoptotic cell-associated antigen. J Exp Med 208(9):1789-97. [PubMed: 21859845]  [MGI Ref ID J:177583]

Feau S; Arens R; Togher S; Schoenberger SP. 2011. Autocrine IL-2 is required for secondary population expansion of CD8(+) memory T cells. Nat Immunol 12(9):908-13. [PubMed: 21804558]  [MGI Ref ID J:176468]

Gerbitz A; Sukumar M; Helm F; Wilke A; Friese C; Fahrenwaldt C; Lehmann FM; Loddenkemper C; Kammertoens T; Mautner J; Schmitt CA; Blankenstein T; Bornkamm GW. 2012. Stromal interferon-gamma signaling and cross-presentation are required to eliminate antigen-loss variants of B cell lymphomas in mice. PLoS One 7(3):e34552. [PubMed: 22479645]  [MGI Ref ID J:187120]

Griffith TS; Brincks EL; Gurung P; Kucaba TA; Ferguson TA. 2011. Systemic immunological tolerance to ocular antigens is mediated by TRAIL-expressing CD8+ T cells. J Immunol 186(2):791-8. [PubMed: 21169546]  [MGI Ref ID J:168759]

Jacobs JP; Wu HJ; Benoist C; Mathis D. 2009. IL-17-producing T cells can augment autoantibody-induced arthritis. Proc Natl Acad Sci U S A :. [PubMed: 19955422]  [MGI Ref ID J:155519]

Janssen EM; Droin NM; Lemmens EE; Pinkoski MJ; Bensinger SJ; Ehst BD; Griffith TS; Green DR; Schoenberger SP. 2005. CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death. Nature 434(7029):88-93. [PubMed: 15744305]  [MGI Ref ID J:96586]

Kang J; Huddleston SJ; Fraser JM; Khoruts A. 2008. De novo induction of antigen-specific CD4+CD25+Foxp3+ regulatory T cells in vivo following systemic antigen administration accompanied by blockade of mTOR. J Leukoc Biol 83(5):1230-9. [PubMed: 18270248]  [MGI Ref ID J:134458]

Khanolkar A; Fulton RB; Epping LL; Pham NL; Tifrea D; Varga SM; Harty JT. 2010. T cell epitope specificity and pathogenesis of mouse hepatitis virus-1-induced disease in susceptible and resistant hosts. J Immunol 185(2):1132-41. [PubMed: 20554960]  [MGI Ref ID J:161935]

Kincaid EZ; Che JW; York I; Escobar H; Reyes-Vargas E; Delgado JC; Welsh RM; Karow ML; Murphy AJ; Valenzuela DM; Yancopoulos GD; Rock KL. 2012. Mice completely lacking immunoproteasomes show major changes in antigen presentation. Nat Immunol 13(2):129-35. [PubMed: 22197977]  [MGI Ref ID J:180765]

Krebs P; Barnes MJ; Lampe K; Whitley K; Bahjat KS; Beutler B; Janssen E; Hoebe K. 2009. NK cell-mediated killing of target cells triggers robust antigen-specific T cell-mediated and humoral responses. Blood 113(26):6593-602. [PubMed: 19406986]  [MGI Ref ID J:150150]

Li H; Wu Q; Li J; Yang P; Zhu Z; Luo B; Hsu HC; Mountz JD. 2013. Cutting Edge: Defective Follicular Exclusion of Apoptotic Antigens Due to Marginal Zone Macrophage Defects in Autoimmune BXD2 Mice. J Immunol 190(9):4465-9. [PubMed: 23543760]  [MGI Ref ID J:195504]

Matheu MP; Su Y; Greenberg ML; Blanc CA; Parker I; Scott DW; Cahalan MD. 2012. Toll-like receptor 4-activated B cells out-compete Toll-like receptor 9-activated B cells to establish peripheral immunological tolerance. Proc Natl Acad Sci U S A 109(20):E1258-66. [PubMed: 22511718]  [MGI Ref ID J:184681]

Mayerova D; Parke EA; Bursch LS; Odumade OA; Hogquist KA. 2004. Langerhans cells activate naive self-antigen-specific CD8 T cells in the steady state. Immunity 21(3):391-400. [PubMed: 15357950]  [MGI Ref ID J:93786]

Moldenhauer LM; Keenihan SN; Hayball JD; Robertson SA. 2010. GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice. J Immunol 185(11):7085-96. [PubMed: 20974989]  [MGI Ref ID J:167366]

Pulko V; Liu X; Krco CJ; Harris KJ; Frigola X; Kwon ED; Dong H. 2009. TLR3-stimulated dendritic cells up-regulate B7-H1 expression and influence the magnitude of CD8 T cell responses to tumor vaccination. J Immunol 183(6):3634-41. [PubMed: 19710456]  [MGI Ref ID J:152305]

Reboulet RA; Hennies CM; Garcia Z; Nierkens S; Janssen EM. 2010. Prolonged antigen storage endows merocytic dendritic cells with enhanced capacity to prime anti-tumor responses in tumor-bearing mice. J Immunol 185(6):3337-47. [PubMed: 20720209]  [MGI Ref ID J:163539]

Seillet C; Jackson JT; Markey KA; Brady HJ; Hill GR; Macdonald KP; Nutt SL; Belz GT. 2013. CD8alpha+ DCs can be induced in the absence of transcription factors Id2, Nfil3, and Batf3. Blood 121(9):1574-83. [PubMed: 23297132]  [MGI Ref ID J:194760]

Wang X; Li H; Matte-Martone C; Cui W; Li N; Tan HS; Roopenian D; Shlomchik WD. 2011. Mechanisms of antigen presentation to T cells in murine graft-versus-host disease: cross-presentation and the appearance of cross-presentation. Blood 118(24):6426-37. [PubMed: 21963602]  [MGI Ref ID J:179099]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	As preliminary attempts to make a homozygous colony at The Jackson Laboratory yielded smaller than normal homozygous males (September 2006), this strain is maintained by breeding hemizygotes to wildtype siblings or C57BL/6J mice.
Mating System	Hemizygote x +/+ sibling         (Female x Male)   07-JUN-08
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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