Strain Name:

C57BL/6-Tg(CAG-OVA)916Jen/J

Stock Number:

005145

Order this mouse

Availability:

Repository- Live

Ovalbumin expression in mice of this strain is detected immunohistochemically on the surfaces of cells of all organs and functions in skin-graft experiments as a minor histocompatibility antigen that triggers graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Description

Strain Information

Former Names C57BL/6-Tg(ACTB-OVA)916Jen/J    (Changed: 08-MAY-08 )
C57BL/6-Tg(Actb-OVA)916Jen/J    (Changed: 29-MAR-05 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHemizygote x +/+ sibling         (Female x Male)   07-JUN-08
Specieslaboratory mouse
GenerationN?+N15 (17-JUL-13)
Generation Definitions
 
Donating Investigator Marc Jenkins,   University of Minnesota

Description
Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-Kb complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-Ab complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Development
A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2Kb and the transmembrane region of the H-2Db gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of ACTB     (15 strains)

View Strains carrying other alleles of OVA     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(CAG-OVA)916Jen/?

        C57BL/6-Tg(CAG-OVA)916Jen
  • cellular phenotype
  • abnormal cell morphology
    • most cells of the body express a membrane bound form of chicken ovalbumin on their cell surface   (MGI Ref ID J:91742)
  • immune system phenotype
  • abnormal dendritic cell antigen presentation
    • dendritic cells in the skin present an epitope of chicken ovalbumin in complex with the MHC allele Kb   (MGI Ref ID J:91742)
    • splenocytes are able to activate OT-I and OT-II transgenic T cells when cultured in vitro   (MGI Ref ID J:91742)
  • decreased length of allograft survival
    • full thickness tail skin grafted from transgenic mice to C57BL/6 mice have a mean survival time of 33.9 days compared a mean survival time of over 200 days for non-transgenic donors   (MGI Ref ID J:91742)
    • recipient mice show accelerated rejection (18 days) when grafted with transgenic tail skin a second time   (MGI Ref ID J:91742)
    • recipient mice also show accelerated rejection when adoptively transferred with OT-I or OT-II T cells (OT-I = Tg(TcraTcrb)1100Mjb, OT-II = Tg(TcraTcrb)425Cbn)   (MGI Ref ID J:91742)
    • when recipient mice lack T cells (Tcratm1Mom homozygotes), rejection of the transgenic skin grafts fail to occur   (MGI Ref ID J:91742)
    • when recipient mice lack CD8 T cells (Cd8atm1Mak homozygotes), transgenic skin grafts shrink rapidly to 10-30% of their original size but then survive indefinitely   (MGI Ref ID J:91742)
    • when recipient mice lack CD4 T cells (H2-Ab1tm1Gru homozygotes), transgenic skin grafts remain healthy for the first 40 days after which they start progressively contracting with 25% of the grafts being lost by 115 days   (MGI Ref ID J:91742)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: multiple
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(CAG-OVA)916Jen
Allele Name transgene insertion 916, Marc K Jenkins
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Act-mOVA; ActmOVA; Tg(ACTB-OVA)916Jen; Tg(CAG-OVA)916Jen; actOVA; actin-OVA;
Mutation Made By Ben Ehst,   University of Minnesota
Strain of OriginC57BL/6
Expressed Gene OVA, ovalbumin, chicken
Promoter ACTB, actin, beta, human
Molecular Note The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-Kb gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-Db gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. [MGI Ref ID J:91742]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAG-OVA)916Jen-alternate1,

MELT


Tg(CAG-OVA)916Jen-alternate1, Separated PCR
Tg(CAG-OVA)916Jen-alternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ehst BD; Ingulli E; Jenkins MK. 2003. Development of a novel transgenic mouse for the study of interactions between CD4 and CD8 T cells during graft rejection. Am J Transplant 3(11):1355-62. [PubMed: 14525595]  [MGI Ref ID J:91742]

Additional References

Tg(CAG-OVA)916Jen related

Baban B; Chandler PR; Sharma MD; Pihkala J; Koni PA; Munn DH; Mellor AL. 2009. IDO activates regulatory T cells and blocks their conversion into Th17-like T cells. J Immunol 183(4):2475-83. [PubMed: 19635913]  [MGI Ref ID J:151475]

Barnes MJ; Aksoylar H; Krebs P; Bourdeau T; Arnold CN; Xia Y; Khovananth K; Engel I; Sovath S; Lampe K; Laws E; Saunders A; Butcher GW; Kronenberg M; Steinbrecher K; Hildeman D; Grimes HL; Beutler B; Hoebe K. 2010. Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice. J Immunol 184(7):3743-54. [PubMed: 20190135]  [MGI Ref ID J:160090]

Coleman MA; Bridge JA; Lane SW; Dixon CM; Hill GR; Wells JW; Thomas R; Steptoe RJ. 2013. Tolerance induction with gene-modified stem cells and immune-preserving conditioning in primed mice: restricting antigen to differentiated antigen-presenting cells permits efficacy. Blood 121(6):1049-58. [PubMed: 23233664]  [MGI Ref ID J:194614]

Desch AN; Randolph GJ; Murphy K; Gautier EL; Kedl RM; Lahoud MH; Caminschi I; Shortman K; Henson PM; Jakubzick CV. 2011. CD103+ pulmonary dendritic cells preferentially acquire and present apoptotic cell-associated antigen. J Exp Med 208(9):1789-97. [PubMed: 21859845]  [MGI Ref ID J:177583]

Feau S; Arens R; Togher S; Schoenberger SP. 2011. Autocrine IL-2 is required for secondary population expansion of CD8(+) memory T cells. Nat Immunol 12(9):908-13. [PubMed: 21804558]  [MGI Ref ID J:176468]

Gerbitz A; Sukumar M; Helm F; Wilke A; Friese C; Fahrenwaldt C; Lehmann FM; Loddenkemper C; Kammertoens T; Mautner J; Schmitt CA; Blankenstein T; Bornkamm GW. 2012. Stromal interferon-gamma signaling and cross-presentation are required to eliminate antigen-loss variants of B cell lymphomas in mice. PLoS One 7(3):e34552. [PubMed: 22479645]  [MGI Ref ID J:187120]

Griffith TS; Brincks EL; Gurung P; Kucaba TA; Ferguson TA. 2011. Systemic immunological tolerance to ocular antigens is mediated by TRAIL-expressing CD8+ T cells. J Immunol 186(2):791-8. [PubMed: 21169546]  [MGI Ref ID J:168759]

Jacobs JP; Wu HJ; Benoist C; Mathis D. 2009. IL-17-producing T cells can augment autoantibody-induced arthritis. Proc Natl Acad Sci U S A :. [PubMed: 19955422]  [MGI Ref ID J:155519]

Janssen EM; Droin NM; Lemmens EE; Pinkoski MJ; Bensinger SJ; Ehst BD; Griffith TS; Green DR; Schoenberger SP. 2005. CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death. Nature 434(7029):88-93. [PubMed: 15744305]  [MGI Ref ID J:96586]

Kang J; Huddleston SJ; Fraser JM; Khoruts A. 2008. De novo induction of antigen-specific CD4+CD25+Foxp3+ regulatory T cells in vivo following systemic antigen administration accompanied by blockade of mTOR. J Leukoc Biol 83(5):1230-9. [PubMed: 18270248]  [MGI Ref ID J:134458]

Khanolkar A; Fulton RB; Epping LL; Pham NL; Tifrea D; Varga SM; Harty JT. 2010. T cell epitope specificity and pathogenesis of mouse hepatitis virus-1-induced disease in susceptible and resistant hosts. J Immunol 185(2):1132-41. [PubMed: 20554960]  [MGI Ref ID J:161935]

Kincaid EZ; Che JW; York I; Escobar H; Reyes-Vargas E; Delgado JC; Welsh RM; Karow ML; Murphy AJ; Valenzuela DM; Yancopoulos GD; Rock KL. 2012. Mice completely lacking immunoproteasomes show major changes in antigen presentation. Nat Immunol 13(2):129-35. [PubMed: 22197977]  [MGI Ref ID J:180765]

Krebs P; Barnes MJ; Lampe K; Whitley K; Bahjat KS; Beutler B; Janssen E; Hoebe K. 2009. NK cell-mediated killing of target cells triggers robust antigen-specific T cell-mediated and humoral responses. Blood 113(26):6593-602. [PubMed: 19406986]  [MGI Ref ID J:150150]

Li H; Wu Q; Li J; Yang P; Zhu Z; Luo B; Hsu HC; Mountz JD. 2013. Cutting Edge: Defective Follicular Exclusion of Apoptotic Antigens Due to Marginal Zone Macrophage Defects in Autoimmune BXD2 Mice. J Immunol 190(9):4465-9. [PubMed: 23543760]  [MGI Ref ID J:195504]

Liu D; Ferrer IR; Konomos M; Ford ML. 2013. Inhibition of CD8+ T cell-derived CD40 signals is necessary but not sufficient for Foxp3+ induced regulatory T cell generation in vivo. J Immunol 191(4):1957-64. [PubMed: 23858029]  [MGI Ref ID J:205687]

Liu D; Krummey SM; Badell IR; Wagener M; Schneeweis LA; Stetsko DK; Suchard SJ; Nadler SG; Ford ML. 2014. 2B4 (CD244) induced by selective CD28 blockade functionally regulates allograft-specific CD8+ T cell responses. J Exp Med 211(2):297-311. [PubMed: 24493803]  [MGI Ref ID J:208437]

Matheu MP; Su Y; Greenberg ML; Blanc CA; Parker I; Scott DW; Cahalan MD. 2012. Toll-like receptor 4-activated B cells out-compete Toll-like receptor 9-activated B cells to establish peripheral immunological tolerance. Proc Natl Acad Sci U S A 109(20):E1258-66. [PubMed: 22511718]  [MGI Ref ID J:184681]

Mayerova D; Parke EA; Bursch LS; Odumade OA; Hogquist KA. 2004. Langerhans cells activate naive self-antigen-specific CD8 T cells in the steady state. Immunity 21(3):391-400. [PubMed: 15357950]  [MGI Ref ID J:93786]

Moldenhauer LM; Keenihan SN; Hayball JD; Robertson SA. 2010. GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice. J Immunol 185(11):7085-96. [PubMed: 20974989]  [MGI Ref ID J:167366]

Perchellet AL; Jasti S; Petroff MG. 2013. Maternal CD4+ and CD8+ T cell tolerance towards a fetal minor histocompatibility antigen in T cell receptor transgenic mice. Biol Reprod 89(4):102. [PubMed: 24025737]  [MGI Ref ID J:203913]

Pino-Lagos K; Guo Y; Brown C; Alexander MP; Elgueta R; Bennett KA; De Vries V; Nowak E; Blomhoff R; Sockanathan S; Chandraratna RA; Dmitrovsky E; Noelle RJ. 2011. A retinoic acid-dependent checkpoint in the development of CD4+ T cell-mediated immunity. J Exp Med 208(9):1767-75. [PubMed: 21859847]  [MGI Ref ID J:205647]

Pulko V; Liu X; Krco CJ; Harris KJ; Frigola X; Kwon ED; Dong H. 2009. TLR3-stimulated dendritic cells up-regulate B7-H1 expression and influence the magnitude of CD8 T cell responses to tumor vaccination. J Immunol 183(6):3634-41. [PubMed: 19710456]  [MGI Ref ID J:152305]

Ramakrishnan R; Tyurin VA; Veglia F; Condamine T; Amoscato A; Mohammadyani D; Johnson JJ; Zhang LM; Klein-Seetharaman J; Celis E; Kagan VE; Gabrilovich DI. 2014. Oxidized lipids block antigen cross-presentation by dendritic cells in cancer. J Immunol 192(6):2920-31. [PubMed: 24554775]  [MGI Ref ID J:209897]

Reboulet RA; Hennies CM; Garcia Z; Nierkens S; Janssen EM. 2010. Prolonged antigen storage endows merocytic dendritic cells with enhanced capacity to prime anti-tumor responses in tumor-bearing mice. J Immunol 185(6):3337-47. [PubMed: 20720209]  [MGI Ref ID J:163539]

Seillet C; Jackson JT; Markey KA; Brady HJ; Hill GR; Macdonald KP; Nutt SL; Belz GT. 2013. CD8alpha+ DCs can be induced in the absence of transcription factors Id2, Nfil3, and Batf3. Blood 121(9):1574-83. [PubMed: 23297132]  [MGI Ref ID J:194760]

Tay CS; Tagliani E; Collins MK; Erlebacher A. 2013. Cis-acting pathways selectively enforce the non-immunogenicity of shed placental antigen for maternal CD8 T cells. PLoS One 8(12):e84064. [PubMed: 24391885]  [MGI Ref ID J:211114]

Thangavelu G; Gill RG; Boon L; Ellestad KK; Anderson CC. 2013. Control of in vivo collateral damage generated by T cell immunity. J Immunol 191(4):1686-91. [PubMed: 23851694]  [MGI Ref ID J:205689]

Wang X; Li H; Matte-Martone C; Cui W; Li N; Tan HS; Roopenian D; Shlomchik WD. 2011. Mechanisms of antigen presentation to T cells in murine graft-versus-host disease: cross-presentation and the appearance of cross-presentation. Blood 118(24):6426-37. [PubMed: 21963602]  [MGI Ref ID J:179099]

Yamazaki C; Sugiyama M; Ohta T; Hemmi H; Hamada E; Sasaki I; Fukuda Y; Yano T; Nobuoka M; Hirashima T; Iizuka A; Sato K; Tanaka T; Hoshino K; Kaisho T. 2013. Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1. J Immunol 190(12):6071-82. [PubMed: 23670193]  [MGI Ref ID J:204851]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryAs preliminary attempts to make a homozygous colony at The Jackson Laboratory yielded smaller than normal homozygous males (September 2006), this strain is maintained by breeding hemizygotes to wildtype siblings or C57BL/6J mice.
Mating SystemHemizygote x +/+ sibling         (Female x Male)   07-JUN-08
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(CAG-OVA)916Jen  
Price per Pair (US dollars $)Pair Genotype
$311.00Hemizygous for Tg(CAG-OVA)916Jen x Noncarrier  
$311.00Noncarrier x Hemizygous for Tg(CAG-OVA)916Jen  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(CAG-OVA)916Jen  
Price per Pair (US dollars $)Pair Genotype
$404.30Hemizygous for Tg(CAG-OVA)916Jen x Noncarrier  
$404.30Noncarrier x Hemizygous for Tg(CAG-OVA)916Jen  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)