Description

Strain Information

Former Names B6.D2-Chr3P/LusJ    (Changed: 12-JUL-05 ) Type Congenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain DBA/2J H2 Haplotype b Generation N?+F4p

Appearance
black
Related Genotype: a/a

Description
This C57BL/6J congenic carries the proximal portion of Chromosome 3 from DBA/2J. The Mit or SNP (rs) markers in the strain name define the congenic interval.

Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range eight to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. (Iakoubova et al. 2001) GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits.

The Jackson Laboratory is working with Dr. Lusis' group to import and make available additional strains in the panel.

Development
This congenic panel was initiated by a cross of B6D2F1/J (Stock #100006) females to C57BL/6J males. Male progeny at each generation were selected by marker assisted genome scan and were backcrossed to C57BL/6J females to produce the N3-N6 generations. All animals in the panel carry a C57BL/6J Y chromosome. Sibling pairs at the N6 generation were intercrossed to produce homozygotes. Iakoubova OA, et. al. provides a more detailed explanation of the chromosome intervals. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Other Related Strains

	005188	B6.D2-(D13Mit198-D13Mit314)/LusJ
	005187	B6.D2-(D13Mit79-D13Mit198)/LusJ
	005206	B6.D2-(D18Mit33-D18Mit144)/LusJ
	005152	B6.D2-(D2Mit1-rs3681655)/LusJ
	005154	B6.D2-(D2Mit405-D2Mit457)/LusJ
	005167	B6.D2-(D6Mit149-D6Mit15)/LusJ
	005166	B6.D2-(D6Mit86-D6Mit198)/LusJ
	005173	B6.D2-(D8Mit256-D8Mit42)/LusJ
	005174	B6.D2-(D8Mit318-D8Mit56)/LusJ
	005175	B6.D2-(D9Mit329-D9Mit115)/LusJ
	005156	B6.D2-(rs3653796-rs3022971)/LusJ
	005205	B6.D2-(rs3696933-rs3672215)/LusJ

View Other Related Strains     (12 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Genetics Research (Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis)

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		D3Mit149, DNA segment, Chr 3, Massachusetts Institute of Technology 149
Chromosome		3

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Iakoubova OA; Olsson CL; Dains KM; Ross DA; Andalibi A; Lau K; Choi J; Kalcheva I; Cunanan M; Louie J; Nimon V; Machrus M; Bentley LG; Beauheim C; Silvey S; Cavalcoli J; Lusis AJ; West DB. 2001. Genome-tagged mice (GTM): two sets of genome-wide congenic strains. Genomics 74(1):89-104. [PubMed: 11374905]  [MGI Ref ID J:69881]

Additional References

Davis RC; Schadt EE; Smith DJ; Hsieh EW; Cervino AC; van Nas A; Rosales M; Doss S; Meng H; Allayee H; Lusis AJ. 2005. A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background. Genomics 86(3):259-70. [PubMed: 16039824]  [MGI Ref ID J:107521]

Liu D; Singh RP; Khan AH; Bhavsar K; Lusis AJ; Davis RC; Smith DJ. 2003. Identifying loci for behavioral traits using genome-tagged mice. J Neurosci Res 74(4):562-9. [PubMed: 14598300]  [MGI Ref ID J:109357]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Mouse Mutant Resource collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.