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| This strain is one of a set of overlapping congenic strains called genome-tagged mice (GTM). Each member of the panel carries an average of a 23 cM segment (range eight to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.D2-Chr3M/LusJ (Changed: 12-JUL-05 ) Type Congenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain DBA/2J H2 Haplotype b Generation N?+F5 (18-DEC-07) Appearance
black
Related Genotype: a/aDescription
This C57BL/6J congenic carries the middle portion of Chromosome 3 from DBA/2J. The Mit or SNP (rs) markers in the strain name define the congenic interval.Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range eight to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. (Iakoubova et al. 2001) GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits.
The Jackson Laboratory is working with Dr. Lusis' group to import and make available additional strains in the panel.
Development
This congenic panel was initiated by a cross of B6D2F1/J (Stock #100006) females to C57BL/6J males. Male progeny at each generation were selected by marker assisted genome scan and were backcrossed to C57BL/6J females to produce the N3-N6 generations. All animals in the panel carry a C57BL/6J Y chromosome. Sibling pairs at the N6 generation were intercrossed to produce homozygotes. Iakoubova OA, et. al. provides a more detailed explanation of the chromosome intervals. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Other Related Strains
005188 B6.D2-(D13Mit198-D13Mit314)/LusJ 005187 B6.D2-(D13Mit79-D13Mit198)/LusJ 005206 B6.D2-(D18Mit33-D18Mit144)/LusJ 005152 B6.D2-(D2Mit1-rs3681655)/LusJ 005154 B6.D2-(D2Mit405-D2Mit457)/LusJ 005155 B6.D2-(D3Mit149-rs3726528)/LusJ 005167 B6.D2-(D6Mit149-D6Mit15)/LusJ 005166 B6.D2-(D6Mit86-D6Mit198)/LusJ 005173 B6.D2-(D8Mit256-D8Mit42)/LusJ 005174 B6.D2-(D8Mit318-D8Mit56)/LusJ 005175 B6.D2-(D9Mit329-D9Mit115)/LusJ 005205 B6.D2-(rs3696933-rs3672215)/LusJ View Other Related Strains (12 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research (Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis)
Genes & Alleles
Gene & Allele Information
Currently there are no related genes or alleles for this strain.Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Iakoubova OA; Olsson CL; Dains KM; Ross DA; Andalibi A; Lau K; Choi J; Kalcheva I; Cunanan M; Louie J; Nimon V; Machrus M; Bentley LG; Beauheim C; Silvey S; Cavalcoli J; Lusis AJ; West DB. 2001. Genome-tagged mice (GTM): two sets of genome-wide congenic strains. Genomics 74(1):89-104. [PubMed: 11374905] [MGI Ref ID J:69881]
Additional References
Davis RC; Schadt EE; Smith DJ; Hsieh EW; Cervino AC; van Nas A; Rosales M; Doss S; Meng H; Allayee H; Lusis AJ. 2005. A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background. Genomics 86(3):259-70. [PubMed: 16039824] [MGI Ref ID J:107521]
Liu D; Singh RP; Khan AH; Bhavsar K; Lusis AJ; Davis RC; Smith DJ. 2003. Identifying loci for behavioral traits using genome-tagged mice. J Neurosci Res 74(4):562-9. [PubMed: 14598300] [MGI Ref ID J:109357]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Cryopreservation Repository.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Cryopreservation Repository |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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