Description

Strain Information

Former Names RIII/NhsJ    (Changed: 15-DEC-04 ) RIII/Sa    (Changed: 15-DEC-04 ) Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Mating System Sibling x Sibling         (Female x Male)   01-MAR-06 Species laboratory mouse H2 Haplotype g Generation F?+F10 (18-DEC-09) Generation Definitions

Appearance
albino
Related Genotype: A/A Tyr^c/ Tyr ^c

Description
The original RIII strain was known for its high incidence of hormone-dependent mammary tumors transmissible by milk-born MMTV. The RIII/ImrNhsJ substrain is reported to show a pattern of virus expression and tumor incidence similar to the RIII strain (Sarkar, et al. 2004). RIII/ImrNhsJ mice carry the following endogenous MMTV loci: Mtv-6, 8, 14, 17 and possibly 21 (Popken-Harris, et al. 2001). Exogenous MMTV infection leads to the deletion of CD4⁺ Vbeta-2 and Vbeta-8 peripheral and central T cells (Uz-Zaman, et al. 2003). It is possible to modulate mammary tumor incidence using high and low calorie diets (Li, et al. 1994). RIII/ImrNhsJ mice infected with exogenous MMTV may be useful for studies of human breast cancer activated by insertional mutagenesis.

NOTE: Mice distributed by The Jackson Laboratory are not infected with MMTV. Regrettably, we do not have a source for the virus.

Development
The original RIII line was inbred at the Institut du Radium, Paris by Dobrovolskia-Zavadskaia in 1928 (Hilkens, et al. 1981). This substrain was transferred to Dr. Moore at the Institute of Medical Research (Imr) in Camden, New Jersey in 1950, then to Dr. Nural H. Sarkar (Nhs) at the Medical College of Georgia in 1974. Dr. Sarker donated this strain to The Jackson Laboratory in 2004.

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Festing Inbred Strain Characteristics: RIII

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors

Genes & Alleles

Gene & Allele Information

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Sarkar NH; Golovkina T; Uz-Zaman T. 2004. RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus. (Erratum v 78(7):3803) J Virol 78(2):1055-62. [PubMed: 14694140]  [MGI Ref ID J:87504]

Uz-Zaman T; Ignatowicz L; Sarkar NH. 2003. Mouse mammary tumor viruses expressed by RIII/Sa mice with a high incidence of mammary tumors interact with the V beta-2- and V beta-8-specific T cells during viral infection. Virology 314(1):294-304. [PubMed: 14517082]  [MGI Ref ID J:85844]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	To maintain viral infectivity and high viral titer, the donating investigator recommends maintaining the strain by sibling matings of the third gestation or higher. Virgin mice fed a standard diet develop tumors at an incidence of 60-70% by 10-12 months, breeding mice develop tumors at an incidence of 90-100% (Li, et al 1994). Virgin mice on a high calorie (16 kcal/day) low fat (5% corn oil) diet have an increased tumor incidence of 73% (Li, et al 1994).
Mating System	Sibling x Sibling         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and six weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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General inquiries

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