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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Satvir Tevethia, Penn State Univ. College of Medicine Description
This transgenic strain carries a rearranged T cell receptor transgene specific for the H2-Db-restricted SV40 large tumor antigen epitope I (residues 206-215). Flow cytometric analysis of lymphocytes detects approximately 94% of CD8+ T cells are specific for the tumor antigen. Mice that are homozygous for the transgenic insert are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of tumor immunology and peripheral T cell tolerance.Development
Transgenic constructs containing the alpha-chain and beta-chain of the T-cell receptor from the SV40 large tumor antigen epitope I-specific cytoxic T lymphocyte clone Y-1 were coinjected into fertilized B6D2F1/J mouse eggs. Founder line 416 animals were backcrossed to C57BL/6 mice for 13 generations.
Control Information
| Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (35 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TcraY1,TcrbY1)416Tev/0
involves: C57BL/6J * DBA/2J
- immune system phenotype
- abnormal immune tolerance (MGI Ref ID J:84288)
- transgenic mice do not develop epitope I-specific T cells after immunization with T antigen epitope I
This mouse can be used to support research in many areas including:
Tcra relatedImmunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes (class I restricted)
Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcraY1,TcrbY1)416Tev | ||
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| Allele Name | transgene insertion 416, Satvir S Tevethia | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TCR-I; line 416 TCR-I; | ||
| Strain of Origin | (C57BL/6J x DBA/2J)F1/J | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| General Note | Transgenic mouse lines were derived from 2 transgene-expressing founder mice and designated lines 416 and 422. The Tg insert of line 416 was backcrossed onto C57BL/6 to create a congenic strain for use in experiments. | ||
| Molecular Note | Single-cell embryos were coinjected with T-cell receptor alpha and T-cell receptor beta expression vectors containing the rearranged genomic Tcra and Tcrb VJ/V(D)J segments from the cytotoxic T-lymphocyte clone Y-1, which expresses a T cellreceptor specific for the H2-Db-restricted SV40 large tumor antigen epitope I (amino acids 206-215). [MGI Ref ID J:84288] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcraY1,TcrbY1)416Tev, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Staveley-O'Carroll K; Schell TD; Jimenez M; Mylin LM; Tevethia MJ; Schoenberger SP; Tevethia SS. 2003. In vivo ligation of CD40 enhances priming against the endogenous tumor antigen and promotes CD8+ T cell effector function in SV40 T antigen transgenic mice. J Immunol 171(2):697-707. [PubMed: 12847236] [MGI Ref ID J:84288]
Additional References
Tg(TcraY1,TcrbY1)416Tev relatedOtahal P; Knowles BB; Tevethia SS; Schell TD. 2007. Anti-CD40 conditioning enhances the T(CD8) response to a highly tolerogenic epitope and subsequent immunotherapy of simian virus 40 T antigen-induced pancreatic tumors. J Immunol 179(10):6686-95. [PubMed: 17982058] [MGI Ref ID J:131746]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When held in a live colony, the strain is maintained as a hemizygote.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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