Strain Name: |
B6.Cg-Tg(Krt1-15-EGFP)2Cot/J |
|---|---|
Stock Number: |
005244 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Krt15; Tg(Krt1-15-EGFP)2Cot; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator George Cotsarelis, University of Pennsylvania Generation N10+2F5 (31-DEC-07) Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse keratin complex 1, acidic, gene 15 promoter. The high levels of specific transgene expression observed in 'bulge cells' (hair follicle stem cells) allow these cells to be isolated with FACS techniques. This mutant mouse strain may be useful in studies of epithelial stem cells.Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein (EGFP) gene under the control of the mouse keratin complex 1, acidic, gene 15 promoter was injected into fertilized B6SJLF1/J mouse eggs. Founder animals were bred to C57BL/6/J mice and backcrossed to the same for a total of ten generations. (Dec 2004).
Gene & Allele Details
| Allele Symbol | Tg(Krt1-15-EGFP)2Cot | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, George Cotsarelis | ||
| Common Name(s) | K15-EGFP; | ||
| Mutation Made By | Yaping Liu, University of Pennsylvania | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | Hair follicle stem cells ("bulge cells") | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Krt15, keratin 15, mouse, laboratory | ||
| General Note | This transgenic line is derived from one of eight EGFP-expressing founder mice. Skin of line 3 mice exhibited the strongest fluorescence under ultraviolet illumination, lines 2, 14, 27, 41, 42 and 44 exhibited less fluorescence, and line 13 an intermediate level. Cells from mice of four lines were screened by fluorescence activated cell sorting (FACS); as line 2 high EGFP-expressing cells separated well from other cells in the sample, this line was used for further FACS analyses. | ||
| Molecular Note | The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP). The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta). [J:94076] [J:94082] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | This strain is hom by hom |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
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005249 B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedDermatology Research
Skin and Hair Texture Defects
Research Tools
Dermatology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesMorris RJ; Liu Y; Marles L; Yang Z; Trempus C; Li S; Lin JS; Sawicki JA; Cotsarelis G. 2004. Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22(4):411-7. [PubMed: 15024388] [J:94076]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Krt1-15-EGFP)2Cot/J |
| Stock Number: | 005244 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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- Use of these MICE by for-profit entities requires a license from the Trustees of the University of Pennsylvania (INSTITUTION).
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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