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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N10+2F7 (31-DEC-08) Donating Investigator George Cotsarelis, University of Pennsylvania Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse keratin complex 1, acidic, gene 15 promoter. The high levels of specific transgene expression observed in 'bulge cells' (hair follicle stem cells) allow these cells to be isolated with FACS techniques. This mutant mouse strain may be useful in studies of epithelial stem cells.Development
A transgenic construct containing an Enhanced Green Fluorescent Protein (EGFP) gene under the control of the mouse keratin complex 1, acidic, gene 15 promoter was injected into fertilized B6SJLF1/J mouse eggs. Founder animals were bred to C57BL/6/J mice and backcrossed to the same for a total of ten generations. (Dec 2004).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005249 B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDermatology Research
Skin and Hair Texture Defects
Research Tools
Dermatology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Krt1-15-EGFP)2Cot | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, George Cotsarelis | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | K15-EGFP; | ||
| Mutation Made By | Yaping Liu, University of Pennsylvania | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | Hair follicle stem cells ("bulge cells") | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Krt15, keratin 15, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Krt1-15-EGFP)2Cot, transgene insertion 2, George Cotsarelis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | K15-EGFP; | ||
| General Note | This transgenic line is derived from one of eight EGFP-expressing founder mice. Skin of line 3 mice exhibited the strongest fluorescence under ultraviolet illumination, lines 2, 14, 27, 41, 42 and 44 exhibited less fluorescence, and line 13 an intermediate level. Cells from mice of four lines were screened by fluorescence activated cell sorting (FACS); as line 2 high EGFP-expressing cells separated well from other cells in the sample, this line was used for further FACS analyses. | ||
| Molecular Note | The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP). The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta). [MGI Ref ID J:94076] [MGI Ref ID J:94082] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Morris RJ; Liu Y; Marles L; Yang Z; Trempus C; Li S; Lin JS; Sawicki JA; Cotsarelis G. 2004. Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22(4):411-7. [PubMed: 15024388] [MGI Ref ID J:94076]
Additional References
Tg(Krt1-15-EGFP)2Cot relatedFlores I; Canela A; Vera E; Tejera A; Cotsarelis G; Blasco MA. 2008. The longest telomeres: a general signature of adult stem cell compartments. Genes Dev 22(5):654-67. [PubMed: 18283121] [MGI Ref ID J:131719]
Liu Y; Lyle S; Yang Z; Cotsarelis G. 2003. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. J Invest Dermatol 121(5):963-8. [PubMed: 14708593] [MGI Ref ID J:94082]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is hom by hom Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $171.20 Female or Male Homozygous for Tg(Krt1-15-EGFP)2Cot
Pairs /Price (US dollars $) Pair Genotype $342.40 Homozygous for Tg(Krt1-15-EGFP)2Cot x Homozygous for Tg(Krt1-15-EGFP)2Cot
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $222.60 Female or Male Homozygous for Tg(Krt1-15-EGFP)2Cot
Pairs /Price (US dollars $) Pair Genotype $445.20 Homozygous for Tg(Krt1-15-EGFP)2Cot x Homozygous for Tg(Krt1-15-EGFP)2Cot
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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