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Strain Name:

B6.129S4-Grin1tm2Stl/J

Stock Number:

005246

Availability:

Repository- Live

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Genes & Alleles   Grin1;   Grin1tm2Stl;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Susumu Tonegawa,   Massachusetts Institute of Technology
GenerationN9+F4 (30-APR-08)

Strain Description
These mice possess loxP sites flanking approximately 12kb of sequence of the targeted gene that encodes the entire transmembrane domain and C-terminal region. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the CA3 region of the hippocampus (see Stock No. 006474 for example), this mutant mouse strain may be useful in studies of associative memory recall.

When bred to a strain expressing Cre recombinase in the CA1 region of the hippocampus (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of nonspacial memory.

Strain Development
A targeting vector containing the gene sequence, neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. A loxP site was inserted between exons 10 and 11. Another loxP site and a neomycin resistance gene were inserted at the 3' end of the gene, 3kb downstream of the last exon. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 8 generations.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Grin1tm2Stl/Grin1tm2Stl

        involves: 129S4/SvJae * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:37457)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Grin1tm2Stl/Grin1tm2Stl Tg(Camk2a-cre)T29-1Stl/0

        involves: 129S4/SvJae * BALB/c * C57BL/6   (conditional)
  • behavior/neurological phenotype
  • abnormal contextual conditioning (MGI Ref ID J:60730)
    • impaired hippocampus-dependent, but not hippocampus-independent, fear memory that can be rescued with enriched training
  • abnormal object recognition memory (MGI Ref ID J:60730)
    • deficit in novel object recognition memory that can be partially rescued with enriched training
  • abnormal olfactory -discrimination memory (MGI Ref ID J:60730)
    • impaired olfactory-discrimination memory that can be rescued with enriched training
  • abnormal spatial learning (MGI Ref ID J:37457)
    • impaired spatial memory in the hidden-platform Morris water maze test, with some progressive improvement in escape latencies but unimpaired nonspatial learning
  • impaired social transmission of food preference (MGI Ref ID J:60730)
  • nervous system phenotype
  • abnormal excitatory postsynaptic currents (MGI Ref ID J:37457)
    • lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors
  • absence of NMDA -mediated synaptic currents (MGI Ref ID J:37457)
    • lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors, however postsynaptic AMPA receptors as well as presynaptic terminals operate normally
  • reduced long term potentiation (MGI Ref ID J:37457)
    • lack long term potentiation in the CA1 synapses

Grin1tm2Stl/Grin1tm2Stl Tg(Grik4-cre)G32-4Stl/0

        involves: C57BL/6   (conditional)
  • behavior/neurological phenotype
  • abnormal spatial learning (MGI Ref ID J:77718)
    • impaired spatial memory recall after partial cue removal but not under full cue conditions in the Morris water maze and mutants exhibited normal motivation, motor coordination and sensory functions
  • nervous system phenotype
  • abnormal NMDA-mediated synaptic currents (MGI Ref ID J:77718)
    • the 6-cyano-7-dinitroquinoxalline-2,3-dione (DNQX)-insensitive component of the NMDA current was significantly reduced at the recurrent commissural/associational synapse relative to controls
  • abnormal single cell response (MGI Ref ID J:77718)
    • CA1 pyramidal cells showed a significant decrease in complex spike bursting properties (complex spike index and burst spike frequency) when mutants were engaged in open-field foraging
    • CA1 cells showed significant reductions in burst spike frequency, place field size and integrated firing rate after partial cue removal, however showed no significant changes when mutants were returned to an environment with all the distal cues present
    • decreased firing rate of putative CA1 interneurons
  • reduced long term potentiation (MGI Ref ID J:77718)
    • long term potentiation was nearly absent at the commissural/associational (CA)-CA3 synapses in 9/11 mutants

Gene & Allele Details

Allele Symbol Grin1tm2Stl
Allele Name targeted mutation 2, Susumu Tonegawa
Common Name(s) NR1flox; fNR1;
Mutation Made By Susumu Tonegawa,   Massachusetts Institute of Technology
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Grin1, glutamate receptor, ionotropic, NMDA1 (zeta 1)
Chromosome 2
Gene Common Name(s) NMDA1; NMDAR1; NR1; Nmdar;
Molecular Note Insertion of loxP sites flanking the entire transmembrane region (exons 11-21 through the 3' end of the gene). [MGI Ref ID J:37457]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Grin1tm2Stl

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature
Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Receptor Defects (glutamate receptor: NMDA)

Research Tools
Cre-lox System (loxP-flanked Sequences)

Grin1tm2Stl related

Neurobiology Research
Behavioral and Learning Defects

References

Selected Reference(s)

Tsien JZ; Chen DF; Gerber D; Tom C; Mercer EH; Anderson DJ; Mayford M; Kandel ER; Tonegawa S. 1996. Subregion- and cell type-restricted gene knockout in mouse brain [see comments] Cell 87(7):1317-26. [PubMed: 8980237]  [MGI Ref ID J:66344]

Additional References

Price and Supply Information

Strain Name: B6.129S4-Grin1tm2Stl/J
Stock Number: 005246

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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