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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (01-MAY-05)
Generation DefinitionsDonating Investigator David D. Ginty, Johns Hopkins University Description
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.Development
A FRT site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. loxP sites were placed flanking exon 2 and the neomycin resistance/ thymidine kinase genes. This construct was electroporated into 129.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were crossed to C57BL/6 mice and then subsequently crossed to an FLP recombinase expressing strain (B6;SJL-Tg(ACTFLPe)9205Dym/J Stock No. 003800) to remove the selection vector. Mice carrying the floxed allele were backcrossed to C57BL/6 for 10 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Nrp1
005245 B6.129(C)-Nrp1tm1Ddg/J View Strains carrying other alleles of Nrp1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Developmental Biology Research
Neurodevelopmental Defects
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Neurodevelopmental Defects
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Nrp1tm2Ddg | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, David D Ginty | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Npn-1c; Nrp-1flox; Nrp1fl; | ||
| Mutation Made By | Dori (Dorothy) Reimert, Johns Hopkins University | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | GK129 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Nrp1, neuropilin 1 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | BDCA4; CD304; NP-1; NP1; NPN-1; NRP; Neuropilin-1; Npn1; VEGF165R; | ||
| Molecular Note | An FRT-flanked neo cassette was inserted upstream of exon 2 and loxP sites were inserted flanking exon 2 via homologous recombination. The neo cassette was removed by mating with mice expressing germline FlpE recombinase. [MGI Ref ID J:93799] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Nrp1tm2Ddg, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851] [MGI Ref ID J:93799]
Additional References
Nrp1tm2Ddg relatedAcevedo LM; Barillas S; Weis SM; Gothert JR; Cheresh DA. 2008. Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood 111(5):2674-80. [PubMed: 18180379] [MGI Ref ID J:131536]
Cao S; Yaqoob U; Das A; Shergill U; Jagavelu K; Huebert RC; Routray C; Abdelmoneim S; Vasdev M; Leof E; Charlton M; Watts RJ; Mukhopadhyay D; Shah VH. 2010. Neuropilin-1 promotes cirrhosis of the rodent and human liver by enhancing PDGF/TGF-beta signaling in hepatic stellate cells. J Clin Invest 120(7):2379-94. [PubMed: 20577048] [MGI Ref ID J:163782]
Cariboni A; Davidson K; Dozio E; Memi F; Schwarz Q; Stossi F; Parnavelas JG; Ruhrberg C. 2011. VEGF signalling controls GnRH neuron survival via NRP1 independently of KDR and blood vessels. Development 138(17):3723-33. [PubMed: 21828096] [MGI Ref ID J:175836]
Choudhary B; Zhou J; Li P; Thomas S; Kaartinen V; Sucov HM. 2009. Absence of TGFbeta signaling in embryonic vascular smooth muscle leads to reduced lysyl oxidase expression, impaired elastogenesis, and aneurysm. Genesis 47(2):115-21. [PubMed: 19165826] [MGI Ref ID J:147389]
Erskine L; Reijntjes S; Pratt T; Denti L; Schwarz Q; Vieira JM; Alakakone B; Shewan D; Ruhrberg C. 2011. VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron 70(5):951-65. [PubMed: 21658587] [MGI Ref ID J:174966]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Fantin A; Vieira JM; Plein A; Denti L; Fruttiger M; Pollard JW; Ruhrberg C. 2013. NRP1 acts cell autonomously in endothelium to promote tip cell function during sprouting angiogenesis. Blood 121(12):2352-62. [PubMed: 23315162] [MGI Ref ID J:195430]
Hansen W; Hutzler M; Abel S; Alter C; Stockmann C; Kliche S; Albert J; Sparwasser T; Sakaguchi S; Westendorf AM; Schadendorf D; Buer J; Helfrich I. 2012. Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J Exp Med 209(11):2001-16. [PubMed: 23045606] [MGI Ref ID J:190913]
Huber AB; Kania A; Tran TS; Gu C; De Marco Garcia N; Lieberam I; Johnson D; Jessell TM; Ginty DD; Kolodkin AL. 2005. Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron 48(6):949-64. [PubMed: 16364899] [MGI Ref ID J:107594]
Huettl RE; Huber AB. 2011. Cranial nerve fasciculation and Schwann cell migration are impaired after loss of Npn-1. Dev Biol 359(2):230-41. [PubMed: 21925156] [MGI Ref ID J:178497]
Huettl RE; Soellner H; Bianchi E; Novitch BG; Huber AB. 2011. Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb. PLoS Biol 9(2):e1001020. [PubMed: 21364975] [MGI Ref ID J:170679]
Imai T; Yamazaki T; Kobayakawa R; Kobayakawa K; Abe T; Suzuki M; Sakano H. 2009. Pre-target axon sorting establishes the neural map topography. Science 325(5940):585-90. [PubMed: 19589963] [MGI Ref ID J:151078]
Le A; Zielinski R; He C; Crow MT; Biswal S; Tuder RM; Becker PM. 2009. Pulmonary epithelial neuropilin-1 deletion enhances development of cigarette smoke-induced emphysema. Am J Respir Crit Care Med 180(5):396-406. [PubMed: 19520907] [MGI Ref ID J:167963]
Maden CH; Gomes J; Schwarz Q; Davidson K; Tinker A; Ruhrberg C. 2012. NRP1 and NRP2 cooperate to regulate gangliogenesis, axon guidance and target innervation in the sympathetic nervous system. Dev Biol 369(2):277-85. [PubMed: 22790009] [MGI Ref ID J:187616]
Matsuo T; Rossier DA; Kan C; Rodriguez I. 2012. The wiring of Grueneberg ganglion axons is dependent on neuropilin 1. Development 139(15):2783-91. [PubMed: 22745317] [MGI Ref ID J:185650]
Mukouyama YS; Gerber HP; Ferrara N; Gu C; Anderson DJ. 2005. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 132(5):941-952. [PubMed: 15673567] [MGI Ref ID J:96924]
Riese A; Eilert Y; Meyer Y; Arin M; Baron JM; Eming S; Krieg T; Kurschat P. 2012. Epidermal expression of neuropilin 1 protects murine keratinocytes from UVB-induced apoptosis. PLoS One 7(12):e50944. [PubMed: 23251405] [MGI Ref ID J:195670]
Schwarz Q; Vieira JM; Howard B; Eickholt BJ; Ruhrberg C. 2008. Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells. Development 135(9):1605-13. [PubMed: 18356247] [MGI Ref ID J:134486]
Solomon BD; Mueller C; Chae WJ; Alabanza LM; Bynoe MS. 2011. Neuropilin-1 attenuates autoreactivity in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A 108(5):2040-5. [PubMed: 21245328] [MGI Ref ID J:169116]
Torre ER; Gutekunst CA; Gross RE. 2010. Expression by midbrain dopamine neurons of Sema3A and 3F receptors is associated with chemorepulsion in vitro but a mild in vivo phenotype. Mol Cell Neurosci 44(2):135-53. [PubMed: 20298787] [MGI Ref ID J:164137]
Yaqoob U; Cao S; Shergill U; Jagavelu K; Geng Z; Yin M; de Assuncao TM; Cao Y; Szabolcs A; Thorgeirsson S; Schwartz M; Yang JD; Ehman R; Roberts L; Mukhopadhyay D; Shah VH. 2012. Neuropilin-1 stimulates tumor growth by increasing fibronectin fibril assembly in the tumor microenvironment. Cancer Res 72(16):4047-59. [PubMed: 22738912] [MGI Ref ID J:191030]
Zhou J; Pashmforoush M; Sucov HM. 2012. Endothelial neuropilin disruption in mice causes DiGeorge syndrome-like malformations via mechanisms distinct to those caused by loss of Tbx1. PLoS One 7(3):e32429. [PubMed: 22396765] [MGI Ref ID J:186857]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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