Strain Name:

B6.129(SJL)-Nrp1tm2Ddg/J

Stock Number:

005247

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Availability:

Repository- Live

Other products are available, see Purchasing Information for Cryopreserved Embryos

Use Restrictions Apply, see Terms of Use
These mice possess loxP sites flanking exon 2 of the neuropilin 1 (Nrp1) gene and have applications in studies related to cardiovascular and neural system development.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   23-AUG-13
Specieslaboratory mouse
Generation?+pN1pN1 (26-NOV-13)
Generation Definitions
 
Donating Investigator David D. Ginty,   Johns Hopkins University

Description
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to B6.Cg-Tg(Tek-cre)1Ywa/J (Stock No. 008863) endothelial cell specific Cre expression results in mice exhibiting abnormal cardiac development and perinatal lethality.

Development
A FRT site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. loxP sites were placed flanking exon 2 and the neomycin resistance/ thymidine kinase genes. This construct was electroporated into 129.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were crossed to C57BL/6 mice and then subsequently crossed to an FLP recombinase expressing strain (B6;SJL-Tg(ACTFLPe)9205Dym/J Stock No. 003800) to remove the selection vector. Mice carrying the floxed allele were backcrossed to C57BL/6 for 10 generations (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Nrp1
005245   B6.129(C)-Nrp1tm1Ddg/J
View Strains carrying other alleles of Nrp1     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Nrp1tm2Ddg/Nrp1tm2Ddg Tg(Tek-cre)1Ywa/0

        involves: 129 * C57BL/6   (conditional)
  • mortality/aging
  • complete perinatal lethality
    • 25/25 mutant mice died perinatally   (MGI Ref ID J:93799)
  • cardiovascular system phenotype
  • abnormal cardiovascular system morphology
    • exhibit multiple cardiac defects   (MGI Ref ID J:93799)
    • abnormal coronary vessel morphology
      • misplacement of coronary arteries   (MGI Ref ID J:93799)
    • persistent truncus arteriosis   (MGI Ref ID J:93799)
    • ventricular septal defect   (MGI Ref ID J:93799)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences
Neurodevelopmental Defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nrp1tm2Ddg
Allele Name targeted mutation 2, David D Ginty
Allele Type Targeted (Floxed/Frt)
Common Name(s) Npn-1c; Nrp-1flox; Nrp1fl;
Mutation Made By Dori (Dorothy) Reimert,   Johns Hopkins University
Strain of Origin129P2/OlaHsd
ES Cell Line NameGK129
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Nrp1, neuropilin 1
Chromosome 8
Gene Common Name(s) BDCA4; CD304; NP-1; NP1; NPN-1; NRP; Neuropilin-1; Npn1; VEGF165R;
Molecular Note An FRT-flanked neo cassette was inserted upstream of exon 2 and loxP sites were inserted flanking exon 2 via homologous recombination. The neo cassette was removed by mating with mice expressing germline FlpE recombinase. [MGI Ref ID J:93799]

Genotyping

Genotyping Information

Genotyping Protocols

Nrp1tm2Ddg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851]  [MGI Ref ID J:93799]

Additional References

Nrp1tm2Ddg related

Acevedo LM; Barillas S; Weis SM; Gothert JR; Cheresh DA. 2008. Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood 111(5):2674-80. [PubMed: 18180379]  [MGI Ref ID J:131536]

Cao S; Yaqoob U; Das A; Shergill U; Jagavelu K; Huebert RC; Routray C; Abdelmoneim S; Vasdev M; Leof E; Charlton M; Watts RJ; Mukhopadhyay D; Shah VH. 2010. Neuropilin-1 promotes cirrhosis of the rodent and human liver by enhancing PDGF/TGF-beta signaling in hepatic stellate cells. J Clin Invest 120(7):2379-94. [PubMed: 20577048]  [MGI Ref ID J:163782]

Cariboni A; Davidson K; Dozio E; Memi F; Schwarz Q; Stossi F; Parnavelas JG; Ruhrberg C. 2011. VEGF signalling controls GnRH neuron survival via NRP1 independently of KDR and blood vessels. Development 138(17):3723-33. [PubMed: 21828096]  [MGI Ref ID J:175836]

Cerani A; Tetreault N; Menard C; Lapalme E; Patel C; Sitaras N; Beaudoin F; Leboeuf D; De Guire V; Binet F; Dejda A; Rezende FA; Miloudi K; Sapieha P. 2013. Neuron-derived semaphorin 3A is an early inducer of vascular permeability in diabetic retinopathy via neuropilin-1. Cell Metab 18(4):505-18. [PubMed: 24093675]  [MGI Ref ID J:206005]

Choudhary B; Zhou J; Li P; Thomas S; Kaartinen V; Sucov HM. 2009. Absence of TGFbeta signaling in embryonic vascular smooth muscle leads to reduced lysyl oxidase expression, impaired elastogenesis, and aneurysm. Genesis 47(2):115-21. [PubMed: 19165826]  [MGI Ref ID J:147389]

Erskine L; Reijntjes S; Pratt T; Denti L; Schwarz Q; Vieira JM; Alakakone B; Shewan D; Ruhrberg C. 2011. VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron 70(5):951-65. [PubMed: 21658587]  [MGI Ref ID J:174966]

Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698]  [MGI Ref ID J:147285]

Fantin A; Vieira JM; Plein A; Denti L; Fruttiger M; Pollard JW; Ruhrberg C. 2013. NRP1 acts cell autonomously in endothelium to promote tip cell function during sprouting angiogenesis. Blood 121(12):2352-62. [PubMed: 23315162]  [MGI Ref ID J:195430]

Hansen W; Hutzler M; Abel S; Alter C; Stockmann C; Kliche S; Albert J; Sparwasser T; Sakaguchi S; Westendorf AM; Schadendorf D; Buer J; Helfrich I. 2012. Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J Exp Med 209(11):2001-16. [PubMed: 23045606]  [MGI Ref ID J:190913]

Huber AB; Kania A; Tran TS; Gu C; De Marco Garcia N; Lieberam I; Johnson D; Jessell TM; Ginty DD; Kolodkin AL. 2005. Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron 48(6):949-64. [PubMed: 16364899]  [MGI Ref ID J:107594]

Huettl RE; Huber AB. 2011. Cranial nerve fasciculation and Schwann cell migration are impaired after loss of Npn-1. Dev Biol 359(2):230-41. [PubMed: 21925156]  [MGI Ref ID J:178497]

Huettl RE; Soellner H; Bianchi E; Novitch BG; Huber AB. 2011. Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb. PLoS Biol 9(2):e1001020. [PubMed: 21364975]  [MGI Ref ID J:170679]

Imai T; Yamazaki T; Kobayakawa R; Kobayakawa K; Abe T; Suzuki M; Sakano H. 2009. Pre-target axon sorting establishes the neural map topography. Science 325(5940):585-90. [PubMed: 19589963]  [MGI Ref ID J:151078]

Le A; Zielinski R; He C; Crow MT; Biswal S; Tuder RM; Becker PM. 2009. Pulmonary epithelial neuropilin-1 deletion enhances development of cigarette smoke-induced emphysema. Am J Respir Crit Care Med 180(5):396-406. [PubMed: 19520907]  [MGI Ref ID J:167963]

Maden CH; Gomes J; Schwarz Q; Davidson K; Tinker A; Ruhrberg C. 2012. NRP1 and NRP2 cooperate to regulate gangliogenesis, axon guidance and target innervation in the sympathetic nervous system. Dev Biol 369(2):277-85. [PubMed: 22790009]  [MGI Ref ID J:187616]

Matsuo T; Rossier DA; Kan C; Rodriguez I. 2012. The wiring of Grueneberg ganglion axons is dependent on neuropilin 1. Development 139(15):2783-91. [PubMed: 22745317]  [MGI Ref ID J:185650]

Mukouyama YS; Gerber HP; Ferrara N; Gu C; Anderson DJ. 2005. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 132(5):941-952. [PubMed: 15673567]  [MGI Ref ID J:96924]

Riese A; Eilert Y; Meyer Y; Arin M; Baron JM; Eming S; Krieg T; Kurschat P. 2012. Epidermal expression of neuropilin 1 protects murine keratinocytes from UVB-induced apoptosis. PLoS One 7(12):e50944. [PubMed: 23251405]  [MGI Ref ID J:195670]

Schwarz Q; Vieira JM; Howard B; Eickholt BJ; Ruhrberg C. 2008. Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells. Development 135(9):1605-13. [PubMed: 18356247]  [MGI Ref ID J:134486]

Solomon BD; Mueller C; Chae WJ; Alabanza LM; Bynoe MS. 2011. Neuropilin-1 attenuates autoreactivity in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A 108(5):2040-5. [PubMed: 21245328]  [MGI Ref ID J:169116]

Sun LO; Jiang Z; Rivlin-Etzion M; Hand R; Brady CM; Matsuoka RL; Yau KW; Feller MB; Kolodkin AL. 2013. On and off retinal circuit assembly by divergent molecular mechanisms. Science 342(6158):1241974. [PubMed: 24179230]  [MGI Ref ID J:202884]

Torre ER; Gutekunst CA; Gross RE. 2010. Expression by midbrain dopamine neurons of Sema3A and 3F receptors is associated with chemorepulsion in vitro but a mild in vivo phenotype. Mol Cell Neurosci 44(2):135-53. [PubMed: 20298787]  [MGI Ref ID J:164137]

Yaqoob U; Cao S; Shergill U; Jagavelu K; Geng Z; Yin M; de Assuncao TM; Cao Y; Szabolcs A; Thorgeirsson S; Schwartz M; Yang JD; Ehman R; Roberts L; Mukhopadhyay D; Shah VH. 2012. Neuropilin-1 stimulates tumor growth by increasing fibronectin fibril assembly in the tumor microenvironment. Cancer Res 72(16):4047-59. [PubMed: 22738912]  [MGI Ref ID J:191030]

Zhou J; Pashmforoush M; Sucov HM. 2012. Endothelial neuropilin disruption in mice causes DiGeorge syndrome-like malformations via mechanisms distinct to those caused by loss of Tbx1. PLoS One 7(3):e32429. [PubMed: 22396765]  [MGI Ref ID J:186857]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   23-AUG-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Nrp1tm2Ddg  
$232.00Female or MaleHomozygous for Nrp1tm2Ddg  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Nrp1tm2Ddg x Heterozygous for Nrp1tm2Ddg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Cryopreserved

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Nrp1tm2Ddg  
$301.60Female or MaleHomozygous for Nrp1tm2Ddg  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Nrp1tm2Ddg x Heterozygous for Nrp1tm2Ddg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Cryopreserved

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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