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| When hemizygous mice of this strain are crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating RU 486-induced, Cre-mediated targeted deletions. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study epithelial and hair follicle stem cells lineage. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-09 Species laboratory mouse Generation N?+N2F1 (24-SEP-12)
Generation DefinitionsDonating Investigator George Cotsarelis, University of Pennsylvania Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice have a synthetic steroid RU 486 inducible Cre-mediated recombination system driven by the mouse keratin complex 1, acidic, gene 15 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human progesterone receptor. The mutant human progesterone receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, RU 486. Restricted to the cytoplasm, the Cre/PGR protein can only gain access to the nuclear compartment after exposure to RU 486. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating RU 486-induced, Cre-mediated targeted deletions. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study epithelial and hair follicle stem cells lineage.Development
A transgenic construct containing sequence encoding cre/PGR under the control of the mouse keratin complex 1, acidic, gene 15 promoter, was introduced into B6SJLF1 donor eggs.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Krt15
005244 B6.Cg-Tg(Krt1-15-EGFP)2Cot/J View Strains carrying other alleles of Krt15 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Progesterone Resistance (PGR)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Krt1-15-cre/PGR)22Cot | ||
|---|---|---|---|
| Allele Name | transgene insertion 22, George Cotsarelis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | K15-CrePR1; K15CrePR; Krt15-cre/PGR)22Cot; | ||
| Mutation Made By | Yaping Liu, University of Pennsylvania | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | when crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating RU 486-induced, cre-mediated targeted deletions | ||
| Expressed Gene | PGR, progesterone receptor, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Krt15, keratin 15, mouse, laboratory | ||
| Driver Note | Krt15 | ||
| General Note | This transgenic line is one of seven founder lines expressing the transgene, as assessed by RT-PCR. Mice of each line were crossed to mice expressing a beta-galactosidase Cre reporter transgene. Following topical application of RU486, bitransgenic mice of lines 22, 42 and 49 were shown to exhibit beta-galactosidase activity in their skin. Line 22, which showed the least enzyme activity in untreated skin, was selected for further analysis. | ||
| Inducible Note | induced by RU486 | ||
| Molecular Note | The transgene comprises the entire 5.0-kb mouse Krt1-15 promoter region driving expression of a fusion protein composed of an amino-terminal nuclear localization signal followed by Cre recombinase joined to the carboxyl-terminal hormone binding domain (HBD) of a mutant human progesterone receptor. This mutant HBD, whose carboxyl terminal is truncated by 42 amino acids, fails to bind endogenous progestins but binds and, paradoxically, is activated by progesterone receptor antagonists such as RU486. Constitutive expression of the fusion protein is directed by the Krt1-15 promoter to epithelial stem cells in the bulge region of the hair follicle. It remains inactive in the cytoplasm until bound to RU486, whereupon it translocates to the nucleus and can catalyze loxP-mediated recombination. [MGI Ref ID J:84610] [MGI Ref ID J:94076] [MGI Ref ID J:94082] | ||
| Gene Symbol and Name | Tg(Krt1-15-cre/PGR)22Cot, transgene insertion 22, George Cotsarelis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | K15-CrePR1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Morris RJ; Liu Y; Marles L; Yang Z; Trempus C; Li S; Lin JS; Sawicki JA; Cotsarelis G. 2004. Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22(4):411-7. [PubMed: 15024388] [MGI Ref ID J:94076]
Additional References
Kellendonk C; Tronche F; Monaghan AP; Angrand PO; Stewart F; Schutz G. 1996. Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acids Res 24(8):1404-11. [PubMed: 8628671] [MGI Ref ID J:84610]
Tg(Krt1-15-cre/PGR)22Cot relatedBornstein S; White R; Malkoski S; Oka M; Han G; Cleaver T; Reh D; Andersen P; Gross N; Olson S; Deng C; Lu SL; Wang XJ. 2009. Smad4 loss in mice causes spontaneous head and neck cancer with increased genomic instability and inflammation. J Clin Invest 119(11):3408-19. [PubMed: 19841536] [MGI Ref ID J:154600]
Chen YT; Tsai MS; Yang TL; Ku AT; Huang KH; Huang CY; Chou FJ; Fan HH; Hong JB; Yen ST; Wang WL; Lin CC; Hsu YC; Su KY; Su IC; Jang CW; Behringer RR; Favaro R; Nicolis SK; Chien CL; Lin SW; Yu IS. 2012. R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. PLoS One 7(9):e46171. [PubMed: 23049968] [MGI Ref ID J:191943]
Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676] [MGI Ref ID J:146637]
Grachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145] [MGI Ref ID J:173930]
Ito M; Liu Y; Yang Z; Nguyen J; Liang F; Morris RJ; Cotsarelis G. 2005. Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis. Nat Med 11(12):1351-4. [PubMed: 16288281] [MGI Ref ID J:146740]
Kandyba E; Leung Y; Chen YB; Widelitz R; Chuong CM; Kobielak K. 2013. Competitive balance of intrabulge BMP/Wnt signaling reveals a robust gene network ruling stem cell homeostasis and cyclic activation. Proc Natl Acad Sci U S A 110(4):1351-6. [PubMed: 23292934] [MGI Ref ID J:193718]
Karnik P; Tekeste Z; McCormick TS; Gilliam AC; Price VH; Cooper KD; Mirmirani P. 2009. Hair follicle stem cell-specific PPARgamma deletion causes scarring alopecia. J Invest Dermatol 129(5):1243-57. [PubMed: 19052558] [MGI Ref ID J:150977]
Kellendonk C; Tronche F; Monaghan AP; Angrand PO; Stewart F; Schutz G. 1996. Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acids Res 24(8):1404-11. [PubMed: 8628671] [MGI Ref ID J:84610]
Kim DJ; Kataoka K; Rao D; Kiguchi K; Cotsarelis G; Digiovanni J. 2009. Targeted disruption of stat3 reveals a major role for follicular stem cells in skin tumor initiation. Cancer Res 69(19):7587-94. [PubMed: 19738054] [MGI Ref ID J:153631]
Lapouge G; Youssef KK; Vokaer B; Achouri Y; Michaux C; Sotiropoulou PA; Blanpain C. 2011. Identifying the cellular origin of squamous skin tumors. Proc Natl Acad Sci U S A 108(18):7431-6. [PubMed: 21502497] [MGI Ref ID J:172048]
Lengner CJ; Camargo FD; Hochedlinger K; Welstead GG; Zaidi S; Gokhale S; Scholer HR; Tomilin A; Jaenisch R. 2007. Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1(4):403-415. [PubMed: 18159219] [MGI Ref ID J:130248]
Liu B; Xia X; Zhu F; Park E; Carbajal S; Kiguchi K; DiGiovanni J; Fischer SM; Hu Y. 2008. IKKalpha is required to maintain skin homeostasis and prevent skin cancer. Cancer Cell 14(3):212-25. [PubMed: 18772111] [MGI Ref ID J:141162]
Liu Y; Lyle S; Yang Z; Cotsarelis G. 2003. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. J Invest Dermatol 121(5):963-8. [PubMed: 14708593] [MGI Ref ID J:94082]
Nakrieko KA; Rudkouskaya A; Irvine TS; D'Souza SJ; Dagnino L. 2011. TARGETED INACTIVATION OF INTEGRIN-LINKED KINASE IN HAIR FOLLICLE STEM CELLS REVEALS AN IMPORTANT MODULATORY ROLE IN SKIN REPAIR AFTER INJURY. Mol Biol Cell :. [PubMed: 21593206] [MGI Ref ID J:172933]
Rabbani P; Takeo M; Chou W; Myung P; Bosenberg M; Chin L; Taketo MM; Ito M. 2011. Coordinated activation of wnt in epithelial and melanocyte stem cells initiates pigmented hair regeneration. Cell 145(6):941-55. [PubMed: 21663796] [MGI Ref ID J:173080]
Sachs N; Secades P; van Hulst L; Kreft M; Song JY; Sonnenberg A. 2012. Loss of integrin alpha3 prevents skin tumor formation by promoting epidermal turnover and depletion of slow-cycling cells. Proc Natl Acad Sci U S A 109(52):21468-73. [PubMed: 23236172] [MGI Ref ID J:193173]
Teta M; Choi YS; Okegbe T; Wong G; Tam OH; Chong MM; Seykora JT; Nagy A; Littman DR; Andl T; Millar SE. 2012. Inducible deletion of epidermal Dicer and Drosha reveals multiple functions for miRNAs in postnatal skin. Development 139(8):1405-16. [PubMed: 22434867] [MGI Ref ID J:183487]
Van Keymeulen A; Mascre G; Youseff KK; Harel I; Michaux C; De Geest N; Szpalski C; Achouri Y; Bloch W; Hassan BA; Blanpain C. 2009. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J Cell Biol 187(1):91-100. [PubMed: 19786578] [MGI Ref ID J:163216]
Wang GY; Wang J; Mancianti ML; Epstein EH Jr. 2011. Basal cell carcinomas arise from hair follicle stem cells in Ptch1(+/-) mice. Cancer Cell 19(1):114-24. [PubMed: 21215705] [MGI Ref ID J:168256]
White AC; Tran K; Khuu J; Dang C; Cui Y; Binder SW; Lowry WE. 2011. Defining the origins of Ras/p53-mediated squamous cell carcinoma. Proc Natl Acad Sci U S A 108(18):7425-30. [PubMed: 21502519] [MGI Ref ID J:172216]
Wong SY; Reiter JF. 2011. From the Cover: Wounding mobilizes hair follicle stem cells to form tumors. Proc Natl Acad Sci U S A 108(10):4093-8. [PubMed: 21321207] [MGI Ref ID J:170482]
Youssef KK; Van Keymeulen A; Lapouge G; Beck B; Michaux C; Achouri Y; Sotiropoulou PA; Blanpain C. 2010. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol 12(3):299-305. [PubMed: 20154679] [MGI Ref ID J:195334]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred with noncarrier (wildtype) siblings. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Krt1-15-cre/PGR)22Cot
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Krt1-15-cre/PGR)22Cot x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Krt1-15-cre/PGR)22Cot Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Krt1-15-cre/PGR)22Cot
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Krt1-15-cre/PGR)22Cot x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Krt1-15-cre/PGR)22Cot Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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