Description

Strain Information

Former Names C57BL/6J-Clcn1nmf310/J    (Changed: 19-MAY-06 ) NMF310    (Changed: 29-MAR-05 ) Type Chemically Induced Mutation; Coisogenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype b

Description
View strain phenotype and additional information on the Neuroscience Mutagenesis Facility web page for Clcn1^adr-mto6J entry.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Clcn1

003817	B6;129S-Fcgr2btm1Ttk Clcn1adr-mto5J/J
003922	BALB/cByJ-Clcn1adr-mto2J jgl/J
001265	BALB/cByJ-Clcn1adr-mto2J/J
005465	C57BL/6J-Clcn1adr-mto7J/J
008253	C57BL/6J-Clcn1adr-mto9J/J
000939	SWR/J-Clcn1adr-mto/J

View Strains carrying other alleles of Clcn1     (6 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Clcn1^adr-mto6J/Clcn1^adr-mto6J

        C57BL/6J-Clcn1^adr-mto6J

• reproductive system phenotype
• infertility (MGI Ref ID J:87349)
  • mice do not breed
• behavior/neurological phenotype
• forelimb paralysis (MGI Ref ID J:87349)
  • front limbs appear to be affected to a lesser degree than hindlimbs with temporary loss of function
• hindlimb paralysis (MGI Ref ID J:87349)
  • hind limbs of these mutants appear to lose function intermittently, i.e. both or one or the other hind leg may lag behind, or may be stretched to the side or front during movement
  • episodes usually last less than a minute, and the mice then move around quite normally
  • when the mutants are lifted up by their tail, the hind legs come together and point down- or backwards

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Clcn1^adr-mto6J related

Cell Biology Research
Channel and Transporter Defects (chloride)

Neurobiology Research
Channel and Transporter Defects (chloride)
Neuroscience Mutagenesis Facility Strain

Genes & Alleles

Gene & Allele Information

Allele Symbol		Clcn1adr-mto6J
Allele Name		myotonia 6 Jackson
Allele Type		Chemically induced (ENU)
Common Name(s)		Clcn1nmf310; NMF310; neuroscience mutagenesis facility 310;
Strain of Origin		C57BL/6J
Gene Symbol and Name		Clcn1, chloride channel 1
Chromosome		6
Gene Common Name(s)		CLC1; Clc-1; Clc1; MGC138361; MGC142055; NMF355; SMCC; SMCC1; adr; arrested development of righting response; mto; myotonia; neuroscience mutagenesis facility, 355; nmf310; nmf355;
General Note		The hind limbs of these mutants appear to lose function intermittently, i.e. both or one or the other hind leg may lag behind, or may be stretched to the side or front during movement. These episodes usually last less than a minute, and the mice then move around quite normally. Front limbs appear to be affected to a lesser degree. When the mutants are lifted up by their tail, the hind legs come together and point down- or backwards. The mutants do not breed and the colony has to be maintained through ovarian transplants.
Molecular Note		Complementation tests between NMF310 and Clcn1adr-mto were conducted. The results of heterozygous x heterozygous matings (NMF310 x BALB/cByj-Clcn1adr-mto2J), i.e. 6 affected mice in a total of 29 progeny, suggest that NMF310 represents an allele of Clcn1.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Clcn1^adr-mto6J related

JAX Neuroscience Mutagenesis Facility URL: http://www.jax.org/nmf. 2004. Heritable mouse mutants from JAX NMF ENU Mutagenesis Program MGI Direct Data Submission :.  [MGI Ref ID J:87349]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Neuroscience Mutagenesis Facility (NMF).

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.