Strain Name:

B6.129S7-Itgaltm1Bll/J

Stock Number:

005257

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Homozygotes exhibit peripheral leukocytosis due to an increased number of neutrophils with defective adhesion properties. This mutant mouse strain may be useful in studies of leukocyte adhesion deficiency type I (LADI), and neutrophil adhesion and extravasation.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Generation[N13p]F12 (24-MAR-11)
Generation Definitions
 
Donating Investigator Christie M. Ballantyne,   Baylor College of Medicine

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by flow cytometry analysis of isolated neutrophils. Homozygotes exhibit peripheral leukocytosis due to an increased number of neutrophils. Isolated neutrophils do not exhibit increased adhesion to purified ICAM-1 or to endothelial cells. Neutrophil extravasation in response to TNF-alpha is diminished in mutant mice. Isolated neutrophils show decreased attachment strength to endothelial cells as revealed by shear stress detachment tests. This mutant mouse strain may be useful in studies of leukocyte adhesion deficiency type I (LADI), and neutrophil adhesion and extravasation.

Development
A targeting vector containing neomycin resistance gene driven by the mouse RNA polymerase II promoter was used to disrupt a 2.1 kb region containing exons 1 and 2. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2 derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The donating investigator stated that the resulting LFA-1 mutant mice (also called Cd11a or Itgal mutant mice) were subsequently backcrossed to C57BL/6 (from Harlan Sprague Dawley) (see SNP note below) for 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, males were bred with C57BL/6J to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Itgaltm1Bll allele
016898   NOD.129S7(B6)-Itgaltm1Bll/CgkJ
View Strains carrying   Itgaltm1Bll     (1 strain)

Strains carrying other alleles of Itgal
014148   C57BL/6-Itgaltm1.1Mshi/J
View Strains carrying other alleles of Itgal     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Itgaltm1Bll/Itgaltm1Bll

        B6.129S7-Itgaltm1Bll
  • immune system phenotype
  • abnormal leukocyte adhesion
    • leukocyte adhesion efficiency in inflamed vessels is significantly reduced compared to wildtype, homozygous Icam1tm1Alb, and homozygous Itgamtm1Bll mice   (MGI Ref ID J:100242)
    • 32% of leukocytes adhere to endothelium compared to 95% in wildtype, with adherence decreasing to 5% in the presence of Icam1 antibodies   (MGI Ref ID J:100242)
  • abnormal leukocyte tethering or rolling
    • treatment with Icam1 antibodies increases leukocyte rolling velocity after TNF-alpha stimulation in venules   (MGI Ref ID J:100242)
  • decreased T cell proliferation
    • reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes   (MGI Ref ID J:90954)
  • impaired natural killer cell mediated cytotoxicity
    • NK cell cytotoxicity was reduced   (MGI Ref ID J:90954)
  • increased leukocyte cell number
    • total leukocyte numbers, polymophonuclear cell numbers and mononuclear cell numbers are increased   (MGI Ref ID J:100242)
    • increased neutrophil cell number
      • 4-fold increase in the number of circulating neutrophils   (MGI Ref ID J:100242)
  • hematopoietic system phenotype
  • abnormal leukocyte adhesion
    • leukocyte adhesion efficiency in inflamed vessels is significantly reduced compared to wildtype, homozygous Icam1tm1Alb, and homozygous Itgamtm1Bll mice   (MGI Ref ID J:100242)
    • 32% of leukocytes adhere to endothelium compared to 95% in wildtype, with adherence decreasing to 5% in the presence of Icam1 antibodies   (MGI Ref ID J:100242)
  • abnormal leukocyte tethering or rolling
    • treatment with Icam1 antibodies increases leukocyte rolling velocity after TNF-alpha stimulation in venules   (MGI Ref ID J:100242)
  • decreased T cell proliferation
    • reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes   (MGI Ref ID J:90954)
  • impaired natural killer cell mediated cytotoxicity
    • NK cell cytotoxicity was reduced   (MGI Ref ID J:90954)
  • increased leukocyte cell number
    • total leukocyte numbers, polymophonuclear cell numbers and mononuclear cell numbers are increased   (MGI Ref ID J:100242)
    • increased neutrophil cell number
      • 4-fold increase in the number of circulating neutrophils   (MGI Ref ID J:100242)
  • cellular phenotype
  • abnormal leukocyte adhesion
    • leukocyte adhesion efficiency in inflamed vessels is significantly reduced compared to wildtype, homozygous Icam1tm1Alb, and homozygous Itgamtm1Bll mice   (MGI Ref ID J:100242)
    • 32% of leukocytes adhere to endothelium compared to 95% in wildtype, with adherence decreasing to 5% in the presence of Icam1 antibodies   (MGI Ref ID J:100242)
  • abnormal leukocyte tethering or rolling
    • treatment with Icam1 antibodies increases leukocyte rolling velocity after TNF-alpha stimulation in venules   (MGI Ref ID J:100242)

Itgaltm1Bll/Itgaltm1Bll

        B6.129S7-Itgaltm1Bll/J
  • immune system phenotype
  • abnormal inflammatory response
    • following exposure to LPS then challenge with TNF at the same site to initiate a local Shwartman response, mice fail to exhibit 20% less hemorrhage and 12% less accumulation of neutrophils compared to the thrombohemorrhagic vasculitis observed in wild type mice   (MGI Ref ID J:113463)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Itgaltm1Bll/Itgaltm1Bll

        involves: 129S7/SvEvBrd * C57BL/6J
  • immune system phenotype
  • abnormal leukocyte migration
    • leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 67%   (MGI Ref ID J:83226)
    • abnormal cellular extravasation
      • neutrophil extravasation in response to TNF-alpha is dramatically decreased   (MGI Ref ID J:83226)
    • abnormal leukocyte adhesion
      • adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils   (MGI Ref ID J:83226)
      • however, normal adhesion of neutrophils to fibrinogen and KLH   (MGI Ref ID J:83226)
      • activated neutrophils show a substantial decrease in adhesion in response to shear stress   (MGI Ref ID J:83226)
  • increased leukocyte cell number
    • leukocytosis but do not develop spontaneous infections   (MGI Ref ID J:83226)
    • increased eosinophil cell number   (MGI Ref ID J:83226)
    • increased monocyte cell number   (MGI Ref ID J:83226)
    • increased neutrophil cell number   (MGI Ref ID J:83226)
  • hematopoietic system phenotype
  • abnormal leukocyte migration
    • leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 67%   (MGI Ref ID J:83226)
    • abnormal cellular extravasation
      • neutrophil extravasation in response to TNF-alpha is dramatically decreased   (MGI Ref ID J:83226)
    • abnormal leukocyte adhesion
      • adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils   (MGI Ref ID J:83226)
      • however, normal adhesion of neutrophils to fibrinogen and KLH   (MGI Ref ID J:83226)
      • activated neutrophils show a substantial decrease in adhesion in response to shear stress   (MGI Ref ID J:83226)
  • increased leukocyte cell number
    • leukocytosis but do not develop spontaneous infections   (MGI Ref ID J:83226)
    • increased eosinophil cell number   (MGI Ref ID J:83226)
    • increased monocyte cell number   (MGI Ref ID J:83226)
    • increased neutrophil cell number   (MGI Ref ID J:83226)
  • cellular phenotype
  • abnormal leukocyte migration
    • leukocyte influx in a subcutaneous air pouch in response to TNF-alpha is reduced by 67%   (MGI Ref ID J:83226)
    • abnormal cellular extravasation
      • neutrophil extravasation in response to TNF-alpha is dramatically decreased   (MGI Ref ID J:83226)
    • abnormal leukocyte adhesion
      • adhesion of neutrophils to ICAM-1 or endothelial cells is not increased with Zas stimulation as observed with wild-type neutrophils   (MGI Ref ID J:83226)
      • however, normal adhesion of neutrophils to fibrinogen and KLH   (MGI Ref ID J:83226)
      • activated neutrophils show a substantial decrease in adhesion in response to shear stress   (MGI Ref ID J:83226)

Itgaltm1Bll/Itgaltm1Bll

        involves: 129S7/SvEvBrd
  • immune system phenotype
  • abnormal cellular extravasation
    • mice have lower accumulation of neutrophils in the bronchoalveolar lavage fluid after LPS administration compared to wild-type mice   (MGI Ref ID J:141063)
  • abnormal leukocyte adhesion
    • leukocytes from these mutant mice fail to have enhanced adhesion to endothelial cells lacking Edil3 (Del-1) expression   (MGI Ref ID J:141063)
  • abnormal neutrophil physiology
    • mice have lower accumulation of neutrophils in the bronchoalveolar lavage fluid after LPS administration compared to wild-type mice   (MGI Ref ID J:141063)
  • cellular phenotype
  • abnormal cellular extravasation
    • mice have lower accumulation of neutrophils in the bronchoalveolar lavage fluid after LPS administration compared to wild-type mice   (MGI Ref ID J:141063)
  • abnormal leukocyte adhesion
    • leukocytes from these mutant mice fail to have enhanced adhesion to endothelial cells lacking Edil3 (Del-1) expression   (MGI Ref ID J:141063)
  • hematopoietic system phenotype
  • abnormal cellular extravasation
    • mice have lower accumulation of neutrophils in the bronchoalveolar lavage fluid after LPS administration compared to wild-type mice   (MGI Ref ID J:141063)
  • abnormal leukocyte adhesion
    • leukocytes from these mutant mice fail to have enhanced adhesion to endothelial cells lacking Edil3 (Del-1) expression   (MGI Ref ID J:141063)
  • abnormal neutrophil physiology
    • mice have lower accumulation of neutrophils in the bronchoalveolar lavage fluid after LPS administration compared to wild-type mice   (MGI Ref ID J:141063)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Itgaltm1Bll related

Cell Biology Research
Defects in Cell Adhesion Molecules

Hematological Research
Neutrophil Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Itgaltm1Bll
Allele Name targeted mutation 1, Christie M Ballantyne
Allele Type Targeted (knock-out)
Common Name(s) CD11alpha KO; Cd11a(-); LFA-1-;
Mutation Made By Huaizhu Wu,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.1
ES Cell Line Strain129S7/SvEvBrd-Hprt
Gene Symbol and Name Itgal, integrin alpha L
Chromosome 7
Gene Common Name(s) CD11A; CD11a antigen; Cd11a; LFA-1; LFA1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte antigen 21;
Molecular Note Exons 1 and 2 were replaced with a neomycin selection casssette inserted by homologous recombination. [MGI Ref ID J:83226]

Genotyping

Genotyping Information

Genotyping Protocols

Itgaltm1Bll, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ding ZM; Babensee JE; Simon SI; Lu H; Perrard JL; Bullard DC; Dai XY; Bromley SK; Dustin ML; Entman ML; Smith CW; Ballantyne CM. 1999. Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration. J Immunol 163(9):5029-38. [PubMed: 10528208]  [MGI Ref ID J:83226]

Wu H; Rodgers JR; Perrard XY; Perrard JL; Prince JE; Abe Y; Davis BK; Dietsch G; Smith CW; Ballantyne CM. 2004. Deficiency of CD11b or CD11d results in reduced staphylococcal enterotoxin-induced T cell response and T cell phenotypic changes. J Immunol 173(1):297-306. [PubMed: 15210787]  [MGI Ref ID J:90954]

Additional References

Itgaltm1Bll related

Arumugam TV; Salter JW; Chidlow JH; Ballantyne CM; Kevil CG; Granger DN. 2004. Contributions of LFA-1 and Mac-1 to brain injury and microvascular dysfunction induced by transient middle cerebral artery occlusion. Am J Physiol Heart Circ Physiol 287(6):H2555-60. [PubMed: 15308480]  [MGI Ref ID J:95752]

Beinke S; Phee H; Clingan JM; Schlessinger J; Matloubian M; Weiss A. 2010. Proline-rich tyrosine kinase-2 is critical for CD8 T-cell short-lived effector fate. Proc Natl Acad Sci U S A 107(37):16234-9. [PubMed: 20805505]  [MGI Ref ID J:164360]

Bose TO; Pham QM; Jellison ER; Mouries J; Ballantyne CM; Lefrancois L. 2013. CD11a Regulates Effector CD8 T Cell Differentiation and Central Memory Development in Response to Infection with Listeria monocytogenes. Infect Immun 81(4):1140-51. [PubMed: 23357382]  [MGI Ref ID J:194055]

Byeseda SE; Burns AR; Dieffenbaugher S; Rumbaut RE; Smith CW; Li Z. 2009. ICAM-1 is necessary for epithelial recruitment of gammadelta T cells and efficient corneal wound healing. Am J Pathol 175(2):571-9. [PubMed: 19608878]  [MGI Ref ID J:150953]

Choi EY; Chavakis E; Czabanka MA; Langer HF; Fraemohs L; Economopoulou M; Kundu RK; Orlandi A; Zheng YY; Prieto DA; Ballantyne CM; Constant SL; Aird WC; Papayannopoulou T; Gahmberg CG; Udey MC; Vajkoczy P; Quertermous T; Dimmeler S; Weber C; Chavakis T. 2008. Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. Science 322(5904):1101-4. [PubMed: 19008446]  [MGI Ref ID J:141063]

Choi EY; Orlova VV; Fagerholm SC; Nurmi SM; Zhang L; Ballantyne CM; Gahmberg CG; Chavakis T. 2008. Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins. Blood 111(7):3607-14. [PubMed: 18239087]  [MGI Ref ID J:133529]

Dunne JL; Collins RG; Beaudet AL; Ballantyne CM; Ley K. 2003. Mac-1, but not LFA-1, uses intercellular adhesion molecule-1 to mediate slow leukocyte rolling in TNF-alpha-induced inflammation. J Immunol 171(11):6105-11. [PubMed: 14634125]  [MGI Ref ID J:100242]

Eskan MA; Jotwani R; Abe T; Chmelar J; Lim JH; Liang S; Ciero PA; Krauss JL; Li F; Rauner M; Hofbauer LC; Choi EY; Chung KJ; Hashim A; Curtis MA; Chavakis T; Hajishengallis G. 2012. The leukocyte integrin antagonist Del-1 inhibits IL-17-mediated inflammatory bone loss. Nat Immunol 13(5):465-73. [PubMed: 22447028]  [MGI Ref ID J:185373]

Frommhold D; Tschada J; Braach N; Buschmann K; Doerner A; Pflaum J; Stahl MS; Wang H; Koch L; Sperandio M; Bierhaus A; Isermann B; Poeschl J. 2011. Protein C Concentrate Controls Leukocyte Recruitment during Inflammation and Improves Survival during Endotoxemia after Efficient in Vivo Activation. Am J Pathol 179(5):2637-50. [PubMed: 21907691]  [MGI Ref ID J:177384]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Glawe JD; Patrick DR; Huang M; Sharp CD; Barlow SC; Kevil CG. 2009. Genetic deficiency of Itgb2 or ItgaL prevents autoimmune diabetes through distinctly different mechanisms in NOD/LtJ mice. Diabetes 58(6):1292-301. [PubMed: 19223596]  [MGI Ref ID J:154351]

Guerau-de-Arellano M; Alroy J; Bullard D; Huber BT. 2005. Aggravated Lyme carditis in CD11a-/- and CD11c-/- mice. Infect Immun 73(11):7637-43. [PubMed: 16239568]  [MGI Ref ID J:104290]

Gultner S; Kuhlmann T; Hesse A; Weber JP; Riemer C; Baier M; Hutloff A. 2010. Reduced Treg frequency in LFA-1-deficient mice allows enhanced T effector differentiation and pathology in EAE. Eur J Immunol 40(12):3403-12. [PubMed: 21108463]  [MGI Ref ID J:174580]

Hirahashi J; Mekala D; Van Ziffle J; Xiao L; Saffaripour S; Wagner DD; Shapiro SD; Lowell C; Mayadas TN. 2006. Mac-1 signaling via Src-family and Syk kinases results in elastase-dependent thrombohemorrhagic vasculopathy. Immunity 25(2):271-83. [PubMed: 16872848]  [MGI Ref ID J:113463]

Hyun YM; Sumagin R; Sarangi PP; Lomakina E; Overstreet MG; Baker CM; Fowell DJ; Waugh RE; Sarelius IH; Kim M. 2012. Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels. J Exp Med :. [PubMed: 22711877]  [MGI Ref ID J:184820]

Keum S; Lee HK; Chu PL; Kan MJ; Huang MN; Gallione CJ; Gunn MD; Lo DC; Marchuk DA. 2013. Natural genetic variation of integrin alpha L (Itgal) modulates ischemic brain injury in stroke. PLoS Genet 9(10):e1003807. [PubMed: 24130503]  [MGI Ref ID J:202928]

Kevil CG; Hicks MJ; He X; Zhang J; Ballantyne CM; Raman C; Schoeb TR; Bullard DC. 2004. Loss of LFA-1, but not Mac-1, protects MRL/MpJ-Fas(lpr) mice from autoimmune disease. Am J Pathol 165(2):609-16. [PubMed: 15277234]  [MGI Ref ID J:91523]

Lange-Sperandio B; Schimpgen K; Rodenbeck B; Chavakis T; Bierhaus A; Nawroth P; Thornhill B; Schaefer F; Chevalier RL. 2006. Distinct roles of Mac-1 and its counter-receptors in neonatal obstructive nephropathy. Kidney Int 69(1):81-8. [PubMed: 16374427]  [MGI Ref ID J:136514]

Lee SH; Prince JE; Rais M; Kheradmand F; Ballantyne CM; Weitz-Schmidt G; Smith CW; Corry DB. 2008. Developmental control of integrin expression regulates th2 effector homing. J Immunol 180(7):4656-67. [PubMed: 18354189]  [MGI Ref ID J:133389]

Li L; Kim JS; Boussiotis VA. 2010. Rap1A regulates generation of T regulatory cells via LFA-1-dependent and LFA-1-independent mechanisms. Cell Immunol 266(1):7-13. [PubMed: 20864093]  [MGI Ref ID J:165410]

Li Z; Burns AR; Rumbaut RE; Smith CW. 2007. {gamma}{delta} T Cells Are Necessary for Platelet and Neutrophil Accumulation in Limbal Vessels and Efficient Epithelial Repair after Corneal Abrasion. Am J Pathol 171(3):838-845. [PubMed: 17675580]  [MGI Ref ID J:124350]

Li Z; Burns AR; Smith CW. 2006. Lymphocyte function-associated antigen-1-dependent inhibition of corneal wound healing. Am J Pathol 169(5):1590-600. [PubMed: 17071583]  [MGI Ref ID J:114562]

Link A; Vogt TK; Favre S; Britschgi MR; Acha-Orbea H; Hinz B; Cyster JG; Luther SA. 2007. Fibroblastic reticular cells in lymph nodes regulate the homeostasis of naive T cells. Nat Immunol 8(11):1255-65. [PubMed: 17893676]  [MGI Ref ID J:126345]

Liu Q; Smith CW; Zhang W; Burns AR; Li Z. 2012. NK cells modulate the inflammatory response to corneal epithelial abrasion and thereby support wound healing. Am J Pathol 181(2):452-62. [PubMed: 22728064]  [MGI Ref ID J:186416]

McDonald B; Pittman K; Menezes GB; Hirota SA; Slaba I; Waterhouse CC; Beck PL; Muruve DA; Kubes P. 2010. Intravascular danger signals guide neutrophils to sites of sterile inflammation. Science 330(6002):362-6. [PubMed: 20947763]  [MGI Ref ID J:164871]

Onishi Y; Fehervari Z; Yamaguchi T; Sakaguchi S. 2008. Foxp3+ natural regulatory T cells preferentially form aggregates on dendritic cells in vitro and actively inhibit their maturation. Proc Natl Acad Sci U S A 105(29):10113-8. [PubMed: 18635688]  [MGI Ref ID J:138325]

Phillipson M; Heit B; Colarusso P; Liu L; Ballantyne CM; Kubes P. 2006. Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade. J Exp Med 203(12):2569-75. [PubMed: 17116736]  [MGI Ref ID J:124585]

Prince JE; Brayton CF; Fossett MC; Durand JA; Kaplan SL; Smith CW; Ballantyne CM. 2001. The differential roles of LFA-1 and Mac-1 in host defense against systemic infection with Streptococcus pneumoniae. J Immunol 166(12):7362-9. [PubMed: 11390487]  [MGI Ref ID J:100254]

Ren B; McCrory MA; Pass C; Bullard DC; Ballantyne CM; Xu Y; Briles DE; Szalai AJ. 2004. The virulence function of Streptococcus pneumoniae surface protein A involves inhibition of complement activation and impairment of complement receptor-mediated protection. J Immunol 173(12):7506-12. [PubMed: 15585877]  [MGI Ref ID J:94855]

Stark MA; Huo Y; Burcin TL; Morris MA; Olson TS; Ley K. 2005. Phagocytosis of Apoptotic Neutrophils Regulates Granulopoiesis via IL-23 and IL-17. Immunity 22(3):285-94. [PubMed: 15780986]  [MGI Ref ID J:97026]

Sundd P; Gutierrez E; Koltsova EK; Kuwano Y; Fukuda S; Pospieszalska MK; Groisman A; Ley K. 2012. 'Slings enable neutrophil rolling at high shear. Nature 488(7411):399-403. [PubMed: 22763437]  [MGI Ref ID J:186701]

Tai X; Van Laethem F; Sharpe AH; Singer A. 2007. Induction of autoimmune disease in CTLA-4 / mice depends on a specific CD28 motif that is required for in vivo costimulation. Proc Natl Acad Sci U S A 104(34):13756-61. [PubMed: 17702861]  [MGI Ref ID J:124096]

Watts GM; Beurskens FJ; Martin-Padura I; Ballantyne CM; Klickstein LB; Brenner MB; Lee DM. 2005. Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol 174(6):3668-75. [PubMed: 15749905]  [MGI Ref ID J:97703]

Wohler J; Bullard D; Schoeb T; Barnum S. 2009. LFA-1 is critical for regulatory T cell homeostasis and function. Mol Immunol 46(11-12):2424-8. [PubMed: 19428111]  [MGI Ref ID J:149546]

Wohler JE; Smith SS; Zinn KR; Bullard DC; Barnum SR. 2009. Gammadelta T cells in EAE: early trafficking events and cytokine requirements. Eur J Immunol 39(6):1516-26. [PubMed: 19384874]  [MGI Ref ID J:149481]

Yee NK; Hamerman JA. 2013. beta2 integrins inhibit TLR responses by regulating NF-kappaB pathway and p38 MAPK activation. Eur J Immunol 43(3):779-92. [PubMed: 23310953]  [MGI Ref ID J:193834]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for Itgaltm1Bll  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for Itgaltm1Bll x Homozygous for Itgaltm1Bll  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for Itgaltm1Bll  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for Itgaltm1Bll x Homozygous for Itgaltm1Bll  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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