Strain Name: |
B6.129S2-Tg(APP)8.9Btla/J |
|---|---|
Stock Number: |
005301 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | APP; Tg(APP)8.9Btla; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Bruce Lamb, The Cleveland Clinic Foundation Generation N11+N1F2 (20-DEC-07) Strain Description
These transgenic mice express all mRNA and protein isoforms of the wild-type human amyloid beta (A4) precursor protein, APP. The transgene expression level is equivalent to the level of endogenous mouse amyloid beta (A4) precursor protein (in the homozygous state). The expression pattern of the various protein isoforms of human APP mimics endogenous mouse gene expression patterns. These mice serve as a model for dosage imbalance for APP that occurs in Down syndrome and also provide a unique model to examine the regulation of APP isoforms, APP processing and amyloid beta metabolism and regulation.Strain Development
A 650 kb YAC transgene containing entire human APP gene (and approximately 350 kb flanking sequence) was transfected into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Founder animals were backcrossed to C57BL/6J for 11 generations.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(APP)8.9Btla | ||
|---|---|---|---|
| Allele Name | transgene insertion 8.9, Bruce Lamb | ||
| Common Name(s) | APPYAC; Py8.9; Tg(APP)C9.24Jgh; hABetaPP; | ||
| Mutation Made By | Bruce Lamb, The Cleveland Clinic Foundation | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | APP, amyloid beta (A4) precursor protein, human | ||
| Molecular Note | A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intacttransgene. Quantitative RT-PCR analysis of transgenic and wild type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. [MGI Ref ID J:80947] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Tg(APP) QPCR
| Breeding & Husbandry | The strain is maintained as a homozygote. |
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| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying other alleles of APP
006409 129S1.Cg-Tg(APPSw)40Btla/J 006555 A.129(B6)-Tg(APPSw)40Btla/J 005300 B6.129-Tg(APPSw)40Btla/J 006406 B6.129S4-Tg(APPSwLon)96Btla/J 005864 B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/J 004662 B6.Cg-Tg(PDGFB-APP)5Lms/J 004661 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J 006293 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J 006006 B6.Cg-Tg(Prnp-APP)A-2Dbo/J 006005 B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/J 004462 B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 006472 D2.129(B6)-Tg(APPSw)40Btla/J View Strains carrying other alleles of APP (15 strains)
Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Room Number AX11
APP related
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Selected Reference(s)
Additional ReferencesLamb BT; Sisodia SS; Lawler AM; Slunt HH; Kitt CA; Kearns WG; Pearson PL; Price DL; Gearhart JD. 1993. Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected] Nat Genet 5(1):22-30. [PubMed: 8220418] [MGI Ref ID J:80947]
| Strain Name: | B6.129S2-Tg(APP)8.9Btla/J |
| Stock Number: | 005301 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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