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Strain Name:

B6.129S2-Tg(APP)8.9Btla/J

Stock Number:

005301

Availability:

Repository- Live

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Genes & Alleles   APP;   Tg(APP)8.9Btla;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Bruce Lamb,   The Cleveland Clinic Foundation
GenerationN11+N1F2 (20-DEC-07)

Strain Description
These transgenic mice express all mRNA and protein isoforms of the wild-type human amyloid beta (A4) precursor protein, APP. The transgene expression level is equivalent to the level of endogenous mouse amyloid beta (A4) precursor protein (in the homozygous state). The expression pattern of the various protein isoforms of human APP mimics endogenous mouse gene expression patterns. These mice serve as a model for dosage imbalance for APP that occurs in Down syndrome and also provide a unique model to examine the regulation of APP isoforms, APP processing and amyloid beta metabolism and regulation.

Strain Development
A 650 kb YAC transgene containing entire human APP gene (and approximately 350 kb flanking sequence) was transfected into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Founder animals were backcrossed to C57BL/6J for 11 generations.

Related Disease (OMIM) Terms

Alzheimer Disease; AD
Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(APP)8.9Btla/Tg(APP)8.9Btla

        involves: 129S2/SvPas
  • behavior/neurological phenotype
  • abnormal locomotor activation (MGI Ref ID J:96742)
    • transgenic mice display more spontaneous locomotor activity than controls; activity is higher than in Tg(APP)8.9Btla or Tg(SOD1)51Yg mice
  • abnormal short-term object recognition memory (MGI Ref ID J:96742)
    • at 5.5 months of age, mice do not appear to recall platform position from the previous day of training and show a lower learning rate over subsequent trials
    • impairment observed at 10 months of age
  • abnormal spatial learning (MGI Ref ID J:96742)
    • at 9 months, a delay of learning process in Morris water maze is observed compared to controls
  • decreased anxiety-related response (MGI Ref ID J:96742)
    • mice spend less time per entry into dark arms than controlsmice spend less time per entry into dark arms than controls
    • mice exit dark side of dark-light box more often than controls
  • increased exploration in new environment (MGI Ref ID J:96742)
    • exploratory behavior is increased for both familiar and novel objects
  • other phenotype
  • *normal* other phenotype (MGI Ref ID J:96742)
    • no Abeta peptides are detected in 5 month old mice
  • vision/eye phenotype
  • *normal* vision/eye phenotype (MGI Ref ID J:80534)
    • little or no perturbation of the retinas structure or cornea is observed
    • abnormal lens morphology (MGI Ref ID J:80534)
      • numerous bodies with circular profiles are present in all lens sections examined
      • circles are present in lenses from mice >6 months of age; circles are 1-5 um in diameter
      • lenses from 6 and 16 month-old mice contain swollen cortical fiber cells and lens plaques
      • fiber cell nuclei are disorganized in the outer cortical region compared to wild-type
      • lens degeneration is variable in severity and onset; in some cases, one lens is more severely affected than the other side
      • abnormal lens fiber morphology (MGI Ref ID J:80534)
        • individual fiber cells are irregularly shaped and fiber cell arrays are misaligned
        • lens fiber cell membranes are morphologically abnormal
        • mice display age-related fiber cell degeneration and cortical plaque formation

Gene & Allele Details

Allele Symbol Tg(APP)8.9Btla
Allele Name transgene insertion 8.9, Bruce Lamb
Common Name(s) APPYAC; Py8.9; Tg(APP)C9.24Jgh; hABetaPP;
Mutation Made By Bruce Lamb,   The Cleveland Clinic Foundation
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Expressed Gene APP, amyloid beta (A4) precursor protein, human
Promoter APP, amyloid beta (A4) precursor protein, human
Molecular Note A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intacttransgene. Quantitative RT-PCR analysis of transgenic and wild type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. [MGI Ref ID J:80947]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(APP) QPCR

Colony Maintenance

Breeding & HusbandryThe strain is maintained as a homozygote.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of APP     (15 strains)

Additional Web Information

Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

APP related

Mouse/Human Gene Homologs
Alzheimer's

Neurobiology Research
Alzheimer's Disease
Neurodegeneration

References

Selected Reference(s)

Lamb BT; Sisodia SS; Lawler AM; Slunt HH; Kitt CA; Kearns WG; Pearson PL; Price DL; Gearhart JD. 1993. Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected] Nat Genet 5(1):22-30. [PubMed: 8220418]  [MGI Ref ID J:80947]

Additional References

Price and Supply Information

Strain Name: B6.129S2-Tg(APP)8.9Btla/J
Stock Number: 005301

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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