Description

Strain Information

Former Names C57BL/6NCrJ    (Changed: 26-APR-05 ) Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Mating System Inbred x Inbred         (Female x Male)   01-MAR-06 Breeding Considerations This strain is a good breeder. Species laboratory mouse Generation F5pF13 (17-SEP-12) Generation Definitions

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Appearance
black
Related Genotype: a/a

Important Note
This strain is homozygous for Crb1^rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.

Description
This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. 5 SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M (rs3709624) is C; 11-004367508-M (rs3659787) is A; 13-041017317-M (rs3722313) is C; 15-057561875-M (rs3702158) is G; 19-049914266-M (rs3724876) is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles 2005.) This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664).

Development
In 1951 C57BL/6J, then at generation F32, were sent from The Jackson Laboratory to The National Institute of Health where they were maintained via sibling mating for decades. In 1980 this subline reached F126 and in 1984 embryos were cryopreserved at the NIH cryopreservation facility. In approximately 1994 some of these embryos were thawed and maintained via sibling mating and this subline was referred to as B6(e84) to specify that it derived from the embryos frozen in 1984. In September and October of 1997 a new set of embryos were cryopreserved from this thawed line by backcrossing +F6 females to their +F5 fathers. In 2005 some of these embryos frozen in 1997 were sent to The Jackson Laboratory where they were thawed and gave rise to C57BL/6NJ. Thus, C57BL/6NJ is devoid of mutations that may have arisen in any C57BL/6N sublines that remained on the shelf after the 1984 freeze. The embryos received by The Jackson Laboratory were thawed, the colony expanded, and re-frozen immediately. This strain is maintained with frequent replenishment from this frozen stock in order to arrest genetic drift.

Related Strains

C57BL Strains

000665	C57BL/10J
003752	C57BL/10ScNJ
000476	C57BL/10ScSnJ
000666	C57BL/10SnJ
001822	C57BL/10SxJ
001197	C57BL/10WtRkJ
000663	C57BL/6By
001139	C57BL/6ByJ
009123	C57BL/6HaJ
000664	C57BL/6J
000924	C57BL/6JEiJ

View C57BL Strains     (11 strains)

Strains carrying   Crb1^rd8 allele

005711	B6.129P2-Prkcqtm1Litt/J
004852	B6;129-Crb1rd8/J
003392	STOCK Crb1rd8/J

View Strains carrying   Crb1^rd8     (3 strains)

Additional Web Information

JAX^® NOTES, Fall 2008; 511. Influence of Nnt alleles on DIO in C57BL/6 JAX^® Mice.
JAX^® NOTES, Winter 2008; 512. New resource illustrates divergence of C57BL/6 laboratory mouse substrains.
Sequence data is available from the Mouse Genomes Project at the Wellcome Trust Sanger Institute

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Body Weight Information - JAX^® Mice Strain C57BL/6NJ (005304)
(This chart reflects the typical correlation between body weight and age for mice maintained in production colonies at The Jackson Laboratory.)

Mouse Phenome Database
Festing Inbred Strain Characteristics: C57BL

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Leber Congenital Amaurosis 8; LCA8   (CRB1) Pigmented Paravenous Chorioretinal Atrophy; PPCRA   (CRB1) Retinitis Pigmentosa 12; RP12   (CRB1)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
General Purpose

Crb1^rd8 related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Eye Defects

Mouse/Human Gene Homologs
Retinitis pigmentosa 12; Leber congenital amaurosis (CLA)

Sensorineural Research
Retinal Degeneration

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Crb1rd8
Allele Name		retinal degeneration 8
Allele Type		Spontaneous
Common Name(s)		nmf144;
Strain of Origin		C57BL/6J
Gene Symbol and Name		Crb1, crumbs homolog 1 (Drosophila)
Chromosome		1
Gene Common Name(s)		7530426H14Rik; A930008G09Rik; LCA8; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; RP12;
Molecular Note		The mutation in the rd8 mouse has been identified as a single base deletion in the Crb1 gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein. [MGI Ref ID J:85459]

Genotyping

Genotyping Information

Genotyping Protocols

Nnt,

Separated MCA

Nnt, Separated PCR

Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. Nature 477(7364):289-94. [PubMed: 21921910]  [MGI Ref ID J:177037]

Toye AA; Lippiat JD; Proks P; Shimomura K; Bentley L; Hugill A; Mijat V; Goldsworthy M; Moir L; Haynes A; Quarterman J; Freeman HC; Ashcroft FM; Cox RD. 2005. A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice. Diabetologia 48(4):675-86. [PubMed: 15729571]  [MGI Ref ID J:106692]

Additional References

Crb1^rd8 related

Aleman TS; Cideciyan AV; Aguirre GK; Huang WC; Mullins CL; Roman AJ; Sumaroka A; Olivares MB; Tsai FF; Schwartz SB; Vandenberghe LH; Limberis MP; Stone EM; Bell P; Wilson JM; Jacobson SG. 2011. Human CRB1-associated retinal degeneration: comparison with the rd8 Crb1-mutant mouse model. Invest Ophthalmol Vis Sci 52(9):6898-910. [PubMed: 21757580]  [MGI Ref ID J:181396]

Barber AC; Hippert C; Duran Y; West EL; Bainbridge JW; Warre-Cornish K; Luhmann UF; Lakowski J; Sowden JC; Ali RR; Pearson RA. 2013. Repair of the degenerate retina by photoreceptor transplantation. Proc Natl Acad Sci U S A 110(1):354-9. [PubMed: 23248312]  [MGI Ref ID J:192521]

Chang B; Hawes NL; Hurd RE; Davisson MT; Nusinowitz S; Heckenlively JR. 2002. Retinal degeneration mutants in the mouse. Vision Res 42(4):517-25. [PubMed: 11853768]  [MGI Ref ID J:75095]

Chang B; Hawes NL; Hurd RE; Wang J; Howell D; Davisson MT; Roderick TH; Nusinowitz S; Heckenlively JR. 2005. Mouse models of ocular diseases. Vis Neurosci 22(5):587-93. [PubMed: 16332269]  [MGI Ref ID J:156373]

Lakowski J; Baron M; Bainbridge J; Barber AC; Pearson RA; Ali RR; Sowden JC. 2010. Cone and rod photoreceptor transplantation in models of the childhood retinopathy Leber congenital amaurosis using flow-sorted Crx-positive donor cells. Hum Mol Genet :. [PubMed: 20858907]  [MGI Ref ID J:165574]

Mattapallil MJ; Wawrousek EF; Chan CC; Zhao H; Roychoudhury J; Ferguson TA; Caspi RR. 2012. The rd8 mutation of the Crb1 gene is present in vendor lines of C57BL/6N mice and embryonic stem cells, and confounds ocular induced mutant phenotypes. Invest Ophthalmol Vis Sci :. [PubMed: 22447858]  [MGI Ref ID J:182353]

Mehalow AK; Kameya S; Smith RS; Hawes NL; Denegre JM; Young JA; Bechtold L; Haider NB; Tepass U; Heckenlively JR; Chang B; Naggert JK; Nishina PM. 2003. CRB1 is essential for external limiting membrane integrity and photoreceptor morphogenesis in the mammalian retina. Hum Mol Genet 12(17):2179-89. [PubMed: 12915475]  [MGI Ref ID J:85459]

Perkins GA; Scott R; Perez A; Ellisman MH; Johnson JE; Fox DA. 2012. Bcl-xL-mediated remodeling of rod and cone synaptic mitochondria after postnatal lead exposure: electron microscopy, tomography and oxygen consumption. Mol Vis 18:3029-48. [PubMed: 23288995]  [MGI Ref ID J:192587]

Won J; Shi LY; Hicks W; Wang J; Hurd R; Naggert JK; Chang B; Nishina PM. 2011. Mouse model resources for vision research. J Ophthalmol 2011:391384. [PubMed: 21052544]  [MGI Ref ID J:166679]

Zhang N; Kolesnikov AV; Jastrzebska B; Mustafi D; Sawada O; Maeda T; Genoud C; Engel A; Kefalov VJ; Palczewski K. 2013. Autosomal recessive retinitis pigmentosa E150K opsin mice exhibit photoreceptor disorganization. J Clin Invest 123(1):121-37. [PubMed: 23221340]  [MGI Ref ID J:194158]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX6
Room Number           RB07

Colony Maintenance

Mating System	Inbred x Inbred         (Female x Male)   01-MAR-06
Breeding Considerations	This strain is a good breeder.
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Important Note

This strain is homozygous for Crb1^rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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