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- Description
- Phenotype
- Genes & alleles
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Description
Strain Information
Former Names C.CByJ-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Changed: 27-APR-06 ) C.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Changed: 10-APR-06 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Background Strain BALB/c Donor Strain (C57BL/6J x BALB/c)F1 H2 Haplotype d Generation N11F?+F10 (14-AUG-08) Donating Investigator Linda Sherman, The Scripps Research Institute Appearance
pink-eyed albino
Related Genotype: A/? Tyrc/TyrcDescription
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I moleculeH2-Kd. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-Kd. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days.This mouse is further modified with the Thy1.1 allele, rather than the alternate allele present in C57BL/10, DBA/2, and BALB/c mice. Thus, cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. The presence of Thy1a serves as a marker for following donor CD8+ T cells in vitro.
This transgenic model is useful in the study of T-cell activation, cross presentation of antigens, process of thymic selection, peripheral tolerance and the immune response to influenza.
Development
The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc1 H2d H2-T18c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to BALB/c for 10 generations, prior to crossing to BALB/c-Thy1a congenic for two generations to make Thy1a homozygote. In 2005, The Jackson Laboratory imported the transgenic stock at generation N11F?. A genome scan performed at The Jackson Laboratory in 2005 confirms the strain background to be BALB/cByJ and FACS analysis confirms Thy1a allele is homozygous.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Thy1a allele
005895 B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J 001317 B6.Cg-Igha Thy1a Gpi1a/J 005023 B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J 000406 B6.PL-Thy1a/CyJ 000983 B6.PL/(84NS)CyJ 007687 BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J 007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J 005922 CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J 005443 CBy.PL(B6)-Thy1a/ScrJ 005686 NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 004483 NOD.NON-Thy1a/1LtJ 002721 NOD.NON-Thy1a/J 005651 SJL.AK-Thy1a/TseJ 003961 SJL.Cg Thy1a-Noxo1hslt/J View Strains carrying Thy1a (14 strains)
005308 B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 005686 NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 006554 B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 008230 FVB(Cg)-Tg(Thy1-SOD1*G93A)T3Hgrd/J 006143 FVB/N-Tg(Thy1-cre)1Vln/J
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TcraCl4,TcrbCl4)1Shrm/?
B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm
- immune system phenotype
- abnormal CD8-positive T cell differentiation (MGI Ref ID J:99756)
- the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-Kd
- 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-Kd
- most thymocytes also express the transgenic TCR
- abnormal T cell subpopulation ratio (MGI Ref ID J:99756)
- skewing toward the development of single-positive CD8 T cells is evident in the thymus
- there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes
- hematopoietic system phenotype
- abnormal CD8-positive T cell differentiation (MGI Ref ID J:99756)
- the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-Kd
- 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-Kd
- most thymocytes also express the transgenic TCR
- abnormal T cell subpopulation ratio (MGI Ref ID J:99756)
- skewing toward the development of single-positive CD8 T cells is evident in the thymus
- there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes
This mouse can be used to support research in many areas including:
Tcra relatedImmunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes
Research Tools
Cancer Research (tumor immunology)
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Thy1a relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcraCl4,TcrbCl4)1Shrm | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Linda Sherman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | (HA)-TCR; CL4; CL4-TCR; Clone 4; Clone-4 TCR; clone 4 TCR; | ||
| Mutation Made By | Linda Sherman, The Scripps Research Institute | ||
| Strain of Origin | (C57BL/6 x BALB/c)F1 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| Molecular Note | Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc1 H2d H2-t18c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer EH, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, EH, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. [MGI Ref ID J:90982] [MGI Ref ID J:99756] | ||
| Allele Symbol | Thy1a | ||
| Allele Name | a variant | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | Thy-1.1; Thy1.1; Thy1a; | ||
| Gene Symbol and Name | Thy1, thymus cell antigen 1, theta | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | CD7; CD90; FLJ33325; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; | ||
| General Note |
The Thy1 locus determines an antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experimentsdesigned to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900). | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcraCl4,TcrbCl4)1Shrm, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Morgan DJ; Kurts C; Kreuwel HT; Holst KL; Heath WR; Sherman LA. 1999. Ontogeny of T cell tolerance to peripherally expressed antigens. Proc Natl Acad Sci U S A 96(7):3854-8. [PubMed: 10097127] [MGI Ref ID J:109899]
Additional References
Thy1a relatedTg(TcraCl4,TcrbCl4)1Shrm relatedBeck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036] [MGI Ref ID J:128325]
Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866] [MGI Ref ID J:107466]
D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183] [MGI Ref ID J:8111]
Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063] [MGI Ref ID J:113109]
Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38. [MGI Ref ID J:13675]
Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin. [MGI Ref ID J:110093]
Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918] [MGI Ref ID J:128250]
Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058] [MGI Ref ID J:128303]
Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198] [MGI Ref ID J:133382]
Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634] [MGI Ref ID J:119110]
Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566] [MGI Ref ID J:126126]
Zaleski M; Klein J. 1974. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol 113(4):1170-7. [PubMed: 4606643] [MGI Ref ID J:5487]
Zaleski MB. 1975. Immune response of mice to the Thy-1.1 antigen: effect of the Ir-5 alleles studies in 129/J and B10.129(6M) mice Immunogenetics 2:241-8. [MGI Ref ID J:30773]
Bercovici N; Heurtier A; Vizler C; Pardigon N; Cambouris C; Desreumaux P; Liblau R. 2000. Systemic administration of agonist peptide blocks the progression of spontaneous CD8-mediated autoimmune diabetes in transgenic mice without bystander damage. J Immunol 165(1):202-10. [PubMed: 10861053] [MGI Ref ID J:62874]
Brown DM; Dilzer AM; Meents DL; Swain SL. 2006. CD4 T cell-mediated protection from lethal influenza: perforin and antibody-mediated mechanisms give a one-two punch. J Immunol 177(5):2888-98. [PubMed: 16920924] [MGI Ref ID J:139502]
Bunt SK; Yang L; Sinha P; Clements VK; Leips J; Ostrand-Rosenberg S. 2007. Reduced inflammation in the tumor microenvironment delays the accumulation of myeloid-derived suppressor cells and limits tumor progression. Cancer Res 67(20):10019-26. [PubMed: 17942936] [MGI Ref ID J:126013]
Caminschi I; Ahmet F; Heger K; Brady J; Nutt SL; Vremec D; Pietersz S; Lahoud MH; Schofield L; Hansen DS; O'Keeffe M; Smyth MJ; Bedoui S; Davey GM; Villadangos JA; Heath WR; Shortman K. 2007. Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells. J Exp Med 204(11):2579-90. [PubMed: 17923506] [MGI Ref ID J:126110]
Cornet A; Savidge TC; Cabarrocas J; Deng WL; Colombel JF; Lassmann H; Desreumaux P; Liblau RS. 2001. Enterocolitis induced by autoimmune targeting of enteric glial cells: a possible mechanism in Crohn's disease? Proc Natl Acad Sci U S A 98(23):13306-11. [PubMed: 11687633] [MGI Ref ID J:131274]
Gallina G; Dolcetti L; Serafini P; De Santo C; Marigo I; Colombo MP; Basso G; Brombacher F; Borrello I; Zanovello P; Bicciato S; Bronte V. 2006. Tumors induce a subset of inflammatory monocytes with immunosuppressive activity on CD8+ T cells. J Clin Invest 116(10):2777-90. [PubMed: 17016559] [MGI Ref ID J:114986]
Griseri T; Beaudoin L; Novak J; Mars LT; Lepault F; Liblau R; Lehuen A. 2005. Invariant NKT cells exacerbate type 1 diabetes induced by CD8 T cells. J Immunol 175(4):2091-101. [PubMed: 16081775] [MGI Ref ID J:107517]
Grosso JF; Kelleher CC; Harris TJ; Maris CH; Hipkiss EL; De Marzo A; Anders R; Netto G; Getnet D; Bruno TC; Goldberg MV; Pardoll DM; Drake CG. 2007. LAG-3 regulates CD8+ T cell accumulation and effector function in murine self- and tumor-tolerance systems. J Clin Invest 117(11):3383-92. [PubMed: 17932562] [MGI Ref ID J:127434]
Hernandez J; Aung S; Redmond WL; Sherman LA. 2001. Phenotypic and functional analysis of CD8(+) T cells undergoing peripheral deletion in response to cross-presentation of self-antigen. J Exp Med 194(6):707-17. [PubMed: 11560988] [MGI Ref ID J:100011]
Huang X; Yang Y. 2006. The fate of effector CD8 T cells in vivo is controlled by the duration of antigen stimulation. Immunology 118(3):361-71. [PubMed: 16827897] [MGI Ref ID J:111931]
Janicki CN; Jenkinson SR; Williams NA; Morgan DJ. 2008. Loss of CTL function among high-avidity tumor-specific CD8+ T cells following tumor infiltration. Cancer Res 68(8):2993-3000. [PubMed: 18413769] [MGI Ref ID J:133958]
Kousis PC; Henderson BW; Maier PG; Gollnick SO. 2007. Photodynamic therapy enhancement of antitumor immunity is regulated by neutrophils. Cancer Res 67(21):10501-10. [PubMed: 17974994] [MGI Ref ID J:127142]
Kreuwel HT; Morgan DJ; Krahl T; Ko A; Sarvetnick N; Sherman LA. 1999. Comparing the relative role of perforin/granzyme versus Fas/Fas ligand cytotoxic pathways in CD8+ T cell-mediated insulin-dependent diabetes mellitus. J Immunol 163(8):4335-41. [PubMed: 10510373] [MGI Ref ID J:100013]
Lin KL; Suzuki Y; Nakano H; Ramsburg E; Gunn MD. 2008. CCR2+ monocyte-derived dendritic cells and exudate macrophages produce influenza-induced pulmonary immune pathology and mortality. J Immunol 180(4):2562-72. [PubMed: 18250467] [MGI Ref ID J:131979]
Lu Z; Yuan L; Zhou X; Sotomayor E; Levitsky HI; Pardoll DM. 2000. CD40-independent pathways of T cell help for priming of CD8(+) cytotoxic T lymphocytes. J Exp Med 191(3):541-50. [PubMed: 10662799] [MGI Ref ID J:115118]
Lyman MA; Aung S; Biggs JA; Sherman LA. 2004. A spontaneously arising pancreatic tumor does not promote the differentiation of naive CD8+ T lymphocytes into effector CTL. J Immunol 172(11):6558-67. [PubMed: 15153470] [MGI Ref ID J:90532]
Magnusson FC; Liblau RS; von Boehmer H; Pittet MJ; Lee JW; Turley SJ; Khazaie K. 2008. Direct presentation of antigen by lymph node stromal cells protects against CD8 T-cell-mediated intestinal autoimmunity. Gastroenterology 134(4):1028-37. [PubMed: 18395084] [MGI Ref ID J:136124]
Martinez X; Kreuwel HT; Redmond WL; Trenney R; Hunter K; Rosen H; Sarvetnick N; Wicker LS; Sherman LA. 2005. CD8+ T Cell Tolerance in Nonobese Diabetic Mice Is Restored by Insulin-Dependent Diabetes Resistance Alleles. J Immunol 175(3):1677-85. [PubMed: 16034108] [MGI Ref ID J:100008]
Matsumura S; Wang B; Kawashima N; Braunstein S; Badura M; Cameron TO; Babb JS; Schneider RJ; Formenti SC; Dustin ML; Demaria S. 2008. Radiation-induced CXCL16 release by breast cancer cells attracts effector T cells. J Immunol 181(5):3099-107. [PubMed: 18713980] [MGI Ref ID J:138960]
Morgan DJ; Kreuwel HT; Fleck S; Levitsky HI; Pardoll DM; Sherman LA. 1998. Activation of low avidity CTL specific for a self epitope results in tumor rejection but not autoimmunity. J Immunol 160(2):643-51. [PubMed: 9551898] [MGI Ref ID J:45169]
Morgan DJ; Liblau R; Scott B; Fleck S; McDevitt HO; Sarvetnick N; Lo D; Sherman LA. 1996. CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol 157(3):978-83. [PubMed: 8757600] [MGI Ref ID J:99756]
Morgan DJ; Nugent CT; Raveney BJ; Sherman LA. 2004. In a transgenic model of spontaneous autoimmune diabetes, expression of a protective class II MHC molecule results in thymic deletion of diabetogenic CD8+ T cells. J Immunol 172(2):1000-8. [PubMed: 14707073] [MGI Ref ID J:100010]
Patten PA; Rock EP; Sonoda T; Fazekas de St Groth B; Jorgensen JL; Davis MM. 1993. Transfer of putative complementarity-determining region loops of T cell receptor V domains confers toxin reactivity but not peptide/MHC specificity. J Immunol 150(6):2281-94. [PubMed: 7680688] [MGI Ref ID J:90982]
Quigley M; Huang X; Yang Y. 2008. STAT1 signaling in CD8 T cells is required for their clonal expansion and memory formation following viral infection in vivo. J Immunol 180(4):2158-64. [PubMed: 18250422] [MGI Ref ID J:131918]
Redmond WL; Marincek BC; Sherman LA. 2005. Distinct requirements for deletion versus anergy during CD8 T cell peripheral tolerance in vivo. J Immunol 174(4):2046-53. [PubMed: 15699134] [MGI Ref ID J:100009]
Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708] [MGI Ref ID J:134242]
Saxena A; Bauer J; Scheikl T; Zappulla J; Audebert M; Desbois S; Waisman A; Lassmann H; Liblau RS; Mars LT. 2008. Cutting edge: Multiple sclerosis-like lesions induced by effector CD8 T cells recognizing a sequestered antigen on oligodendrocytes. J Immunol 181(3):1617-21. [PubMed: 18641296] [MGI Ref ID J:137838]
Sepulveda H; Cerwenka A; Morgan T; Dutton RW. 1999. CD28, IL-2-independent costimulatory pathways for CD8 T lymphocyte activation. J Immunol 163(3):1133-42. [PubMed: 10415007] [MGI Ref ID J:118773]
Sinha P; Clements VK; Fulton AM; Ostrand-Rosenberg S. 2007. Prostaglandin E2 promotes tumor progression by inducing myeloid-derived suppressor cells. Cancer Res 67(9):4507-13. [PubMed: 17483367] [MGI Ref ID J:121303]
VanOosten RL; Griffith TS. 2007. Activation of tumor-specific CD8+ T Cells after intratumoral Ad5-TRAIL/CpG oligodeoxynucleotide combination therapy. Cancer Res 67(24):11980-90. [PubMed: 18089829] [MGI Ref ID J:129272]
Wallet MA; Sen P; Flores RR; Wang Y; Yi Z; Huang Y; Mathews CE; Earp HS; Matsushima G; Wang B; Tisch R. 2008. MerTK is required for apoptotic cell-induced T cell tolerance. J Exp Med 205(1):219-32. [PubMed: 18195070] [MGI Ref ID J:131291]
Winau F; Hegasy G; Weiskirchen R; Weber S; Cassan C; Sieling PA; Modlin RL; Liblau RS; Gressner AM; Kaufmann SH. 2007. Ito cells are liver-resident antigen-presenting cells for activating T cell responses. Immunity 26(1):117-29. [PubMed: 17239632] [MGI Ref ID J:118321]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $54.00 Female or Male Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $108.00 Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm x Noncarrier $108.00 Noncarrier x Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm $108.00 Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm x Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $70.20 Female or Male Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $140.40 Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm x Noncarrier $140.40 Noncarrier x Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm $140.40 Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm x Hemizygous for Tg(TcraCl4,TcrbCl4)1Shrm
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
No Liability
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.