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Strain Name:

B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ

Stock Number:

005308

Availability:

Repository-Cryopreserved

Price and Supply Information

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Additional Licensing and Use Restrictions

Former Name      B10.Cg Hc1 H2d H2-T18c-Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ    (Changed: 06-SEP-05 )
      B10.Cg Hc1 H2d H2-T18c-Tg(TcraCl4,TcrbCl4)1Scr/ScrJ    (Changed: 01-SEP-05 )
Genes & Alleles   H2-T18;   H2;   H2d;   Hc;   Hc1;   Tcra;   Tcrb;   Tg(TcraCl4,TcrbCl4)1Shrm;   ;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Major Histocompatibility Congenic
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Background Strain B10.D2-Hc1 H2d H2-T18c/nSnJ
Donor Strain (C57BL/6J x BALB/c)F1
Donating Investigator Linda Sherman,   The Scripps Research Institute
H2 Haplotyped

Appearance
black
Related Genotype: a/a

Strain Description
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-Kd. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-Kd. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days.

This transgenic model is useful in the study of T-cell activation, cross presentation of antigens, process of thymic selection, peripheral tolerance and the immune response to influenza.

Strain Development
The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc1 H2d H2-T18c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to B10.D2-Hc1H2dH2-T18cfor 20 generations. In 2005, the Jackson Laboratory received B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Clone4 TCR) at generation N20 and backcrossed it to B10.D2-Hc1H2dH2-T18c/nSnJ (STOCK# 000463) one generation prior to maintaining by brother-sister matings.

Mammalian Phenotype Terms assigned by genotype

Tg(TcraCl4,TcrbCl4)1Shrm/?

        B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm
  • immune system phenotype
  • abnormal CD8-positive T cell differentiation (MGI Ref ID J:99756)
    • the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-Kd
    • 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-Kd
    • most thymocytes also express the transgenic TCR
  • abnormal T cell subpopulation ratio (MGI Ref ID J:99756)
    • skewing toward the development of single-positive CD8 T cells is evident in the thymus
    • there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes
  • hematopoietic system phenotype
  • abnormal CD8-positive T cell differentiation (MGI Ref ID J:99756)
    • the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-Kd
    • 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-Kd
    • most thymocytes also express the transgenic TCR
  • abnormal T cell subpopulation ratio (MGI Ref ID J:99756)
    • skewing toward the development of single-positive CD8 T cells is evident in the thymus
    • there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

Gene & Allele Details

Allele Symbol H2d
Allele Name d variant
Gene Symbol and Name H2, histocompatibility-2, MHC
Chromosome 17
Gene Common Name(s) H-2; MHC-II;
General Note The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB
 
Allele Symbol Hc1
Allele Name sufficient
Common Name(s) C5 sufficient; C5-s; C5-suf;
Gene Symbol and Name Hc, hemolytic complement
Chromosome 2
Gene Common Name(s) C5; C5a; CPAMD4; FLJ17816; FLJ17822; He; MGC142298; RGD1561905;
General Note

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The C3H/HeJ allele does not have the 2 bp deletion and is associated with resistance to allergen-induced bronchial hyperresponsiveness. (J:108211)

Molecular Note This allele does not contain the 2 base "TA" deletion found in the Hc0 allele and is found in the following mouse strains BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. This is equivalent to the wild-type allele. [MGI Ref ID J:23983]
 
Allele Symbol Tg(TcraCl4,TcrbCl4)1Shrm
Allele Name transgene insertion 1, Linda Sherman
Common Name(s) (HA)-TCR; CL4; CL4-TCR; Clone 4; Clone-4 TCR; clone 4 TCR;
Mutation Made By Linda Sherman,   The Scripps Research Institute
Strain of Origin(C57BL/6 x BALB/c)F1
Expressed Gene Tcrb, T-cell receptor beta chain, mouse, laboratory
Expressed Gene Tcra, T-cell receptor alpha chain, mouse, laboratory
Molecular Note Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc1 H2d H2-t18c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer EH, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, EH, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. [MGI Ref ID J:90982] [MGI Ref ID J:99756]
Gene Symbol and Name H2-T18, histocompatibility 2, T region locus 18
Chromosome 17
Gene Common Name(s) H-2T18; TL Ag; Tla; thymus leukemia antigen;

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Tg(TcraCl4,TcrbCl4)1Shrm

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   H2d     (13 strains)

Strains carrying   Hc1 allele
000470   AK.M-H2m H2-T18a/nSnJ
000463   B10.D2-Hc1 H2d H2-T18c/nSnJ
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
004306   NOD.CBALs-Hc1/LtJ
View Strains carrying   Hc1     (4 strains)

Strains carrying   Tg(TcraCl4,TcrbCl4)1Shrm allele
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
View Strains carrying   Tg(TcraCl4,TcrbCl4)1Shrm     (2 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes

Research Tools
Cancer Research (tumor immunology)
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Tcrb related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

H2d related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Hc1 related
Immunodeficiency (specific complement deficiency) (control)

Research Tools
Immunology and Inflammation Research (specific complement deficiency) (C5 complement) (control)

References

Selected Reference(s)

Morgan DJ; Liblau R; Scott B; Fleck S; McDevitt HO; Sarvetnick N; Lo D; Sherman LA. 1996. CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol 157(3):978-83. [PubMed: 8757600]  [MGI Ref ID J:99756]

Additional References

Price and Supply Information

Strain Name: B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
Stock Number: 005308

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)
Cryorecovery Fee $1900.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Type 1 Diabetes Repository collection.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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