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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names NOD.B6-(D11Nds1-D11Mit325)/DelJ (Changed: 22-JAN-07 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/Wehi Donor Strain C57BL/6J H2 Haplotype g7 Generation N3F18+F5p
Generation DefinitionsDonating Investigator Terry Delovitch, John P, Robarts Research Institute Appearance
albino, pink-eyed
Related Genotype: A/? Tyrc/TyrcImportant Note
Genetic Quality Control of this Chromosome 11, Idd4 congenic stock indicates Chromosome 1 markers in the Idd5 region are of C57BL/6 origin.Description
Surprisingly, Genetic Quality Control completed at the Jackson Laboratory indicates Chromosome 1 markers in the Idd5 region are of C57BL/6 origin and may be viewed at QC of Idd4 congenic stocks.
The donating investigator reports diabetes onset of female NOD.B6-(D1Mit18-D1Mit445) (D11Nds1-D11Mit41)/DelJ (commonly called NOD.B6-Idd4A, Chromosome 11, 43.8-49cM) is delayed, with 15% of the females and 5% of the males becoming diabetic by 30 weeks of age. In the same colony, diabetes rates in NOD controls are 75% in females and 45% in males by 30 weeks of age. Histological examination of 12-week-old NOD.B6-Idd4A mice indicates 70% of the islets are normal compared with 50% in NOD controls. At 25-30 weeks of age, 10% of NOD.B6-Idd4A islets are infiltrated compared to 80% in NOD controls. Until 25-30 weeks of age, NOD.B6-Idd4A mice only develop periductal infiltrates with mild non-destructive insulitis. Additionally, immunohistochemical assays indicate normal amounts of stored insulin. Purified splenic T-cells from 12-week-old, non-diabetic NOD.B6-Idd4A females alone are able to induce diabetes in NOD.Prkdcscid female recipients. Diabetes onset is slightly delayed in recipients of NOD.B6-Idd4A T-cells only, with 50% becoming diabetic by 70 days post transfer compared to 80% diabetic in recipients receiving either diabetic NOD T-cells alone or recipients co-transferred with diabetic NOD/ non-diabetic NOD.B6-Idd4A T-cells.TCR stimulated T-cell proliferative responses or anti-CD3 stimulated splenic T-cells express CD69, CD25, and produce IL2 at similar levels to NOD controls, while producing more IL4 and less interferon gamma than NOD controls (Grattan et al. 2002).The reported conclusion of diabetes resistance has been re-evaluated since the C57BL/6 contaminating genome on Chromosome 1 has been eliminated in Stock No. 006809. Diabetes incidence studies preformed at The Jackson Laboratory show an accelerated onset and incidence of diabetes in Stock No. 006809 females when compared with NOD/ShiLtJ controls and the original Stock No. 005311, while the diabetes onset and incidence in males is accelerated compared to the original Stock No. 005311 and is slightly less compared to NOD/ShiLtJ controls. 006809 incidence study and fine mapping data.
This strain may be useful for identifying genes involved with diabetes resistance located within the Idd4 region of Chromosome 11.Development
The three Idd4 subloci map to Chr. 11. Chr. 11 genomic segments from C57BL/6 were transferred to NOD by outcross to an NODxC57BL/6J F2 mouse followed by 3 backcross cycles to NOD/Del with selection for B6 markers on Chr 11 prior to inbreeding. T.L. Delovitch established three congenic strains based on their congenic intervals, D11Nds1-D11Mit325 (43.8-49cM), D11Mit38-D11Mit325 (49cM) and D11Mit38 (49cM). The 5.2cM region located between D11Nds1 - D11Mit325 of Chr 11 is known to contain Nos2 (45.6cM) Mpmv4 (48cM), Xmv42 (53cM), Mpo, (49cM, D11Mit38) and the beta-chemokine gene family, including, Ccl1 (Tcr3, 47cM), Ccl2(Mcp1, 46.5cM), Ccl3 (Mip1a, 47.59cM), Ccl4 (Mip1b, 47.6cM) (Gill et al, 1995 and Grattan et al, 2002). Additional microsatellite analysis confirm all other known Idd's are NOD in origin (Grattan, et al., 2002). In 2005, The Jackson Laboratory received NOD.B6-(D11Nds1-D11Mit325)/DelJ (NOD.Idd4A) at generation N3F18.Genetic Quality Control data completed at the Jackson Laboratory indicates the following Chromosome 11 markers are of B6 origin: D11Nds1 (43.8cM), D11Mit245 (44.8cM), D11Mit325 (49cM), D11Mit38 (49cM) and D11Mit41 (49cM), while D11Mit339 (31.7cM) and D11Mit358 (52.5cM) are of NOD origin. Surprisingly, Chromosome 1 markers (Idd5 region) of C57BL/6 origin include: D1Mit18 (29.7cM), D1Mit180 (41cM), D1Jmp12 (47-51.4cM), D1Jmp23 (54.6cM) and D1Mit445 (70cM), while D1Mit411 (18.5cM), D1Mit143 (86.6cM) and D1Mit26 (62.1cM) are of NOD origin. Data may be viewed at QC of Idd4 congenic stocks
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Idd4C57BL/6 allele
005313 NOD.B6-(D1Mit180-D1Mit143)(D11Mit38-D11Mit41)/DelJ 005312 NOD.B6-(D1Mit18-D1Jmp12)(D11Mit325-D11Mit41)/DelJ View Strains carrying Idd4C57BL/6 (2 strains)
006809 NOD.B6-(D11Nds1-D11Mit41)/J 005313 NOD.B6-(D1Mit180-D1Mit143)(D11Mit38-D11Mit41)/DelJ 005312 NOD.B6-(D1Mit18-D1Jmp12)(D11Mit325-D11Mit41)/DelJ
006809 NOD.B6-(D11Nds1-D11Mit41)/J
005312 NOD.B6-(D1Mit18-D1Jmp12)(D11Mit325-D11Mit41)/DelJ
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD/ShiLtJ MHC Congenics
Immunology and Inflammation Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation
Genes & Alleles
Gene & Allele Information provided by MGI
| Gene Symbol and Name | D1Mit445, DNA Segment, Chr 1, Massachusetts Institute of Technology 445 | ||
|---|---|---|---|
| Chromosome | 1 | ||
| Gene Symbol and Name | D11Nds1, DNA segment, Chr 11, Nuffield Department of Surgery 1 | ||
| Chromosome | 11 | ||
| Gene Symbol and Name | D1Mit18, DNA segment, Chr 1, Massachusetts Institute of Technology 18 | ||
| Chromosome | 1 | ||
| Gene Symbol and Name | D11Mit41, DNA segment, Chr 11, Massachusetts Institute of Technology 41 | ||
| Chromosome | 11 | ||
| Allele Symbol | Idd4C57BL/6 | ||
| Allele Name | C57BL/6 | ||
| Allele Type | QTL | ||
| Strain of Origin | C57BL/6 | ||
| Gene Symbol and Name | Idd4, insulin dependent diabetes susceptibility 4 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | Idd-4; | ||
References
References provided by MGI
Selected Reference(s)
Gill BM; Jaramillo A; Ma L; Laupland KB; Delovitch TL. 1995. Genetic linkage of thymic T-cell proliferative unresponsiveness to mouse chromosome 11 in NOD mice. A possible role for chemokine genes. Diabetes 44(6):614-9. [PubMed: 7789623] [MGI Ref ID J:25969]
Grattan M; Mi QS; Meagher C; Delovitch TL. 2002. Congenic mapping of the diabetogenic locus Idd4 to a 5.2-cM region of chromosome 11 in NOD mice: identification of two potential candidate subloci. Diabetes 51(1):215-23. [PubMed: 11756344] [MGI Ref ID J:73763]
Additional References
Idd4C57BL/6 relatedMatsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418] [MGI Ref ID J:85823]
Yui MA; Muralidharan K; Moreno-Altamirano B; Perrin G; Chestnut K; Wakeland EK. 1996. Production of congenic mouse strains carrying NOD-derived diabetogenic genetic intervals: an approach for the genetic dissection of complex traits. Mamm Genome 7(5):331-4. [PubMed: 8661724] [MGI Ref ID J:33172]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Important Note
Genetic Quality Control of this Chromosome 11, Idd4 congenic stock indicates Chromosome 1 markers in the Idd5 region are of C57BL/6 origin.
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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