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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N6+F4 (08-JAN-08) Donating Investigator Stefano Piccolo, UNIVERSITY OF PADUA Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These "BAT-GAL" transgenic mice are a reporter strain that express beta-galactosidase in the presence of activated beta-catenin. The transgene expresses the lacZ gene under the control of a regulatory sequence consisting of seven consensus LEF/TCF-binding motifs upstream of the Xenopus siamois gene minimal promoter. Transgenic mice display beta-galactosidase activity beginning at embryonic day 6.0 in the posterior side of the proximal epiblast. Beta-galactosidase expression is detectable in the posterior primitive streak and node at gastrulation, and progresses to the paraxial mesoderm and notochord. Beta-galactosidase activity in developing and adult nervous tissue mimics the pattern of Wnt signaling. When bred to other mutant strains, this reporter strain may be useful for identifying mutations that affect the Wnt-signalling pathway, and in the identification of Wnt-responsive cell populations in development and disease.Development
A transgenic construct containing the lacZ gene under the control of a promoter consisting of seven consensus LEF-/TCF-binding motifs upstream of the 0.13 kb minimal promoter of the Xenopus siamois gene was injected into fertilized B6D2F2 mouse eggs. Founder animals were bred to CD-1 outbred mice. After this, transgenic mice were bred to C57BL/6J mice for 5 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(BAT-lacZ)3Picc/Tg(BAT-lacZ)3Picc
involves: C57BL/6 * DBA/2
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:82727)
- homozygotes are viable, fertile and display no gross physical or behavioral abnormalities
- transgene gives a read-out of beta-catenin activity by expression of beta-galactosidase; expression is initially detectable at E6 in posterior side of proximal epiblast and is observed in the posterior primitive streak and node at gastrulation, progressing to the paraxial mesoderm and notochord
- expression in developing and adult tissues mimics the pattern of Wnt signaling
This mouse can be used to support research in many areas including:
lacZ relatedCell Biology Research
Signal Transduction
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Developmental Biology Research
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(BAT-lacZ)3Picc | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Stefano Piccolo | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | BAT-gal; BATnlacZ; beta-catenin activated transgene driving expression of nuclear beta-galactosidase reporter; | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Site of Expression | beta-galactosidase activity in developing and adult nervous tissue mimics the pattern of Wnt signaling | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | siamois, siamois gene minimal promoter, Xenopus , | ||
| General Note | This is one of three "BAT-gal" transgenic lines generated, which the authors state exhibitied "qualitatively identical expression patterns, although quantitative differences could be observed." | ||
| Molecular Note | Mice with this transgene express nuclear beta-galactosidase from the E. coli lacZ coding sequence under control of the beta-catenin-activated transgene (BAT) promoter. This promoter consists of a DNA fragment extending 0.13 kb upstream from the translation start site of the novel protein containing homeodomain (siamois) gene of Xenopus laevis, which contains the (beta-catenin non-responsive) minimal promoter of this natively beta-catenin activated gene, downstream of seven synthetic lymphoid enhancer factor/T-cell factor- (LEF/TCF-) binding sites that effectively restore the promoter's beta-catenin responsiveness. [MGI Ref ID J:82727] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, MCA, vers. 2
Generic LacZ QPCR, QPCR, vers. 1
Generic LacZ, STD PCR, vers. 1
Tg(0.13sia-lacZ)3Picc, , vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Maretto S; Cordenonsi M; Dupont S; Braghetta P; Broccoli V; Hassan AB; Volpin D; Bressan GM; Piccolo S. 2003. Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors. Proc Natl Acad Sci U S A 100(6):3299-304. [PubMed: 12626757] [MGI Ref ID J:82727]
Additional References
Tg(BAT-lacZ)3Picc relatedAulehla A; Wiegraebe W; Baubet V; Wahl MB; Deng C; Taketo M; Lewandoski M; Pourquie O. 2008. A beta-catenin gradient links the clock and wavefront systems in mouse embryo segmentation. Nat Cell Biol 10(2):186-93. [PubMed: 18157121] [MGI Ref ID J:132361]
Bridgewater D; Cox B; Cain J; Lau A; Athaide V; Gill PS; Kuure S; Sainio K; Rosenblum ND. 2008. Canonical WNT/beta-catenin signaling is required for ureteric branching. Dev Biol 317(1):83-94. [PubMed: 18358465] [MGI Ref ID J:136068]
Cohen ED; Wang Z; Lepore JJ; Lu MM; Taketo MM; Epstein DJ; Morrisey EE. 2007. Wnt/beta-catenin signaling promotes expansion of Isl-1-positive cardiac progenitor cells through regulation of FGF signaling. J Clin Invest 117(7):1794-804. [PubMed: 17607356] [MGI Ref ID J:124189]
Corbit KC; Shyer AE; Dowdle WE; Gaulden J; Singla V; Reiter JF. 2008. Kif3a constrains beta-catenin-dependent Wnt signalling through dual ciliary and non-ciliary mechanisms. Nat Cell Biol 10(1):70-6. [PubMed: 18084282] [MGI Ref ID J:132369]
Goux D; Coudert JD; Maurice D; Scarpellino L; Jeannet G; Piccolo S; Weston K; Huelsken J; Held W. 2005. Cooperating pre-T-cell receptor and TCF-1-dependent signals ensure thymocyte survival. Blood 106(5):1726-33. [PubMed: 15890681] [MGI Ref ID J:118496]
Hammerschmidt B; Schlake T. 2007. Localization of Shh expression by Wnt and Eda affects axial polarity and shape of hairs. Dev Biol 305(1):246-61. [PubMed: 17376426] [MGI Ref ID J:121316]
Kemler R; Hierholzer A; Kanzler B; Kuppig S; Hansen K; Taketo MM; de Vries WN; Knowles BB; Solter D. 2004. Stabilization of {beta}-catenin in the mouse zygote leads to premature epithelial-mesenchymal transition in the epiblast. Development 131(23):5817-5824. [PubMed: 15525667] [MGI Ref ID J:94395]
Kreslova J; Machon O; Ruzickova J; Lachova J; Wawrousek EF; Kemler R; Krauss S; Piatigorsky J; Kozmik Z. 2007. Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function. Genesis 45(4):157-68. [PubMed: 17410548] [MGI Ref ID J:121794]
Lewis SL; Khoo PL; De Young RA; Steiner K; Wilcock C; Mukhopadhyay M; Westphal H; Jamieson RV; Robb L; Tam PP. 2008. Dkk1 and Wnt3 interact to control head morphogenesis in the mouse. Development 135(10):1791-801. [PubMed: 18403408] [MGI Ref ID J:134688]
Li B; Rheaume C; Teng A; Bilanchone V; Munguia JE; Hu M; Jessen S; Piccolo S; Waterman ML; Dai X. 2007. Developmental phenotypes and reduced Wnt signaling in mice deficient for pygopus 2. Genesis 45(5):318-25. [PubMed: 17458864] [MGI Ref ID J:121776]
Liu F; Chu EY; Watt B; Zhang Y; Gallant NM; Andl T; Yang SH; Lu MM; Piccolo S; Schmidt-Ullrich R; Taketo MM; Morrisey EE; Atit R; Dlugosz AA; Millar SE. 2008. Wnt/beta-catenin signaling directs multiple stages of tooth morphogenesis. Dev Biol 313(1):210-24. [PubMed: 18022614] [MGI Ref ID J:130228]
Lorenz K; Grashoff C; Torka R; Sakai T; Langbein L; Bloch W; Aumailley M; Fassler R. 2007. Integrin-linked kinase is required for epidermal and hair follicle morphogenesis. J Cell Biol 177(3):501-13. [PubMed: 17485490] [MGI Ref ID J:134724]
Lu P; Yu Y; Perdue Y; Werb Z. 2008. The apical ectodermal ridge is a timer for generating distal limb progenitors. Development 135(8):1395-405. [PubMed: 18359901] [MGI Ref ID J:136057]
Machon O; Backman M; Machonova O; Kozmik Z; Vacik T; Andersen L; Krauss S. 2007. A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus. Dev Biol 311(1):223-37. [PubMed: 17916349] [MGI Ref ID J:126323]
Narhi K; Jarvinen E; Birchmeier W; Taketo MM; Mikkola ML; Thesleff I. 2008. Sustained epithelial {beta}-catenin activity induces precocious hair development but disrupts hair follicle down-growth and hair shaft formation. Development 135(6):1019-28. [PubMed: 18256193] [MGI Ref ID J:131961]
Qian D; Jones C; Rzadzinska A; Mark S; Zhang X; Steel KP; Dai X; Chen P. 2007. Wnt5a functions in planar cell polarity regulation in mice. Dev Biol 306(1):121-33. [PubMed: 17433286] [MGI Ref ID J:122585]
Schwab KR; Patterson LT; Hartman HA; Song N; Lang RA; Lin X; Potter SS. 2007. Pygo1 and Pygo2 roles in Wnt signaling in mammalian kidney development. BMC Biol 5:15. [PubMed: 17425782] [MGI Ref ID J:121918]
Solberg N; Machon O; Krauss S. 2008. Effect of canonical Wnt inhibition in the neurogenic cortex, hippocampus, and premigratory dentate gyrus progenitor pool. Dev Dyn 237(7):1799-811. [PubMed: 18521945] [MGI Ref ID J:137035]
Zaghetto AA; Paina S; Mantero S; Platonova N; Peretto P; Bovetti S; Puche A; Piccolo S; Merlo GR. 2007. Activation of the Wnt-beta catenin pathway in a cell population on the surface of the forebrain is essential for the establishment of olfactory axon connections. J Neurosci 27(36):9757-68. [PubMed: 17804636] [MGI Ref ID J:124891]
Zhang Y; Goss AM; Cohen ED; Kadzik R; Lepore JJ; Muthukumaraswamy K; Yang J; DeMayo FJ; Whitsett JA; Parmacek MS; Morrisey EE. 2008. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Nat Genet 40(7):862-70. [PubMed: 18536717] [MGI Ref ID J:138407]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $203.80 Female or Male Homozygous for Tg(BAT-lacZ)3Picc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $407.60 Homozygous for Tg(BAT-lacZ)3Picc x Homozygous for Tg(BAT-lacZ)3Picc
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $265.00 Female or Male Homozygous for Tg(BAT-lacZ)3Picc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $529.90 Homozygous for Tg(BAT-lacZ)3Picc x Homozygous for Tg(BAT-lacZ)3Picc
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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