Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Generation N10+N2F2 (06-DEC-11) Generation Definitions   Donating Investigator Prof. Dr. RolfK Kemler,   Max Planck Institute for Immunobiology

Description
These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted into intron 10 of the targeted gene, and another loxP site was inserted into intron 5. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and then backcrossed to the same for 10 generations before being made homozygous.

Control Information

	Control
	See control note:	C57BL/6J mice (Stock No. 000664) may be used as controls.
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cdh1

016933	B6.129P2(Cg)-Cdh1tm1Cle/J
002473	B6;129S2-Cdh1tm1Kem/J

View Strains carrying other alleles of Cdh1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Breast Cancer   (CDH1) Endometrial Cancer   (CDH1) Gastric Cancer, Hereditary Diffuse; HDGC   (CDH1) Ovarian Cancer   (CDH1) Prostate Cancer   (CDH1)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Cdh1^tm2Kem related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cdh1tm2Kem
Allele Name		targeted mutation 2, Rolf Kemler
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		E-cadlox; E-cadherinflox; E-cadherinfloxed; Ecadfl;
Mutation Made By		Wilhelmine de Vries,   The Jackson Laboratory
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Cdh1, cadherin 1
Chromosome		8
Gene Common Name(s)		AA960649; Arc-1; CD324; CDHE; E-cad; E-cadherin; ECAD; Ecad; L-CAM; LCAM; UM; UVO; Um; expressed sequence AA960649; uvomorulin;
Molecular Note		Single loxP sites flanked a region encompassing exons 6 through 10 followed by a floxed selection cassette was excised via in vitro cre mediated recombination. [MGI Ref ID J:77170]

Genotyping

Genotyping Information

Genotyping Protocols

Cdh1^tm2Kem, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Boussadia O; Kutsch S; Hierholzer A; Delmas V; Kemler R. 2002. E-cadherin is a survival factor for the lactating mouse mammary gland. Mech Dev 115(1-2):53-62. [PubMed: 12049767]  [MGI Ref ID J:77170]

Additional References

Cdh1^tm2Kem related

Battle MA; Konopka G; Parviz F; Gaggl AL; Yang C; Sladek FM; Duncan SA. 2006. Hepatocyte nuclear factor 4alpha orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver. Proc Natl Acad Sci U S A 103(22):8419-24. [PubMed: 16714383]  [MGI Ref ID J:110205]

Cali G; Zannini M; Rubini P; Tacchetti C; D'Andrea B; Affuso A; Wintermantel T; Boussadia O; Terracciano D; Silberschmidt D; Amendola E; De Felice M; Schutz G; Kemler R; Di Lauro R; Nitsch L. 2007. Conditional inactivation of the E-cadherin gene in thyroid follicular cells affects gland development but does not impair junction formation. Endocrinology 148(6):2737-46. [PubMed: 17347311]  [MGI Ref ID J:122323]

Ceteci F; Ceteci S; Karreman C; Kramer BW; Asan E; Gotz R; Rapp UR. 2007. Disruption of Tumor Cell Adhesion Promotes Angiogenic Switch and Progression to Micrometastasis in RAF-Driven Murine Lung Cancer. Cancer Cell 12(2):145-159. [PubMed: 17692806]  [MGI Ref ID J:124321]

Chauhan BK; Disanza A; Choi SY; Faber SC; Lou M; Beggs HE; Scita G; Zheng Y; Lang RA. 2009. Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination. Development 136(21):3657-67. [PubMed: 19820184]  [MGI Ref ID J:153957]

Damsky WE; Curley DP; Santhanakrishnan M; Rosenbaum LE; Platt JT; Gould Rothberg BE; Taketo MM; Dankort D; Rimm DL; McMahon M; Bosenberg M. 2011. beta-Catenin Signaling Controls Metastasis in Braf-Activated Pten-Deficient Melanomas. Cancer Cell 20(6):741-54. [PubMed: 22172720]  [MGI Ref ID J:178598]

Fiederling A; Ewert R; Andreyeva A; Jungling K; Gottmann K. 2011. E-cadherin is required at GABAergic synapses in cultured cortical neurons. Neurosci Lett 501(3):167-72. [PubMed: 21782891]  [MGI Ref ID J:175782]

Hofmann M; Pircher H. 2011. E-cadherin promotes accumulation of a unique memory CD8 T-cell population in murine salivary glands. Proc Natl Acad Sci U S A 108(40):16741-6. [PubMed: 21930933]  [MGI Ref ID J:177361]

Hwang S; Zimmerman NP; Agle KA; Turner JR; Kumar SN; Dwinell MB. 2012. E-cadherin is critical for collective sheet migration and is regulated by the chemokine CXCL12 protein during restitution. J Biol Chem 287(26):22227-40. [PubMed: 22549778]  [MGI Ref ID J:187535]

Kan NG; Stemmler MP; Junghans D; Kanzler B; de Vries WN; Dominis M; Kemler R. 2007. Gene replacement reveals a specific role for E-cadherin in the formation of a functional trophectoderm. Development 134(1):31-41. [PubMed: 17138661]  [MGI Ref ID J:117056]

Kanatsu-Shinohara M; Takehashi M; Takashima S; Lee J; Morimoto H; Chuma S; Raducanu A; Nakatsuji N; Fassler R; Shinohara T. 2008. Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. Cell Stem Cell 3(5):533-42. [PubMed: 18983968]  [MGI Ref ID J:149789]

Karpowicz P; Willaime-Morawek S; Balenci L; DeVeale B; Inoue T; van der Kooy D. 2009. E-Cadherin regulates neural stem cell self-renewal. J Neurosci 29(12):3885-96. [PubMed: 19321785]  [MGI Ref ID J:147274]

Li CY; Cha W; Luder HU; Charles RP; McMahon M; Mitsiadis TA; Klein OD. 2012. E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor. Dev Biol 366(2):357-66. [PubMed: 22537490]  [MGI Ref ID J:185423]

Libusova L; Stemmler MP; Hierholzer A; Schwarz H; Kemler R. 2010. N-cadherin can structurally substitute for E-cadherin during intestinal development but leads to polyp formation. Development 137(14):2297-305. [PubMed: 20534673]  [MGI Ref ID J:161852]

Pontoriero GF; Smith AN; Miller LA; Radice GL; West-Mays JA; Lang RA. 2009. Co-operative roles for E-cadherin and N-cadherin during lens vesicle separation and lens epithelial cell survival. Dev Biol 326(2):403-17. [PubMed: 18996109]  [MGI Ref ID J:145191]

Reardon SN; King ML; MacLean JA 2nd; Mann JL; DeMayo FJ; Lydon JP; Hayashi K. 2012. CDH1 is essential for endometrial differentiation, gland development, and adult function in the mouse uterus. Biol Reprod 86(5):141, 1-10. [PubMed: 22378759]  [MGI Ref ID J:185830]

Schneider MR; Dahlhoff M; Horst D; Hirschi B; Trulzsch K; Muller-Hocker J; Vogelmann R; Allgauer M; Gerhard M; Steininger S; Wolf E; Kolligs FT. 2010. A key role for E-cadherin in intestinal homeostasis and Paneth cell maturation. PLoS One 5(12):e14325. [PubMed: 21179475]  [MGI Ref ID J:168992]

Stemmler MP; Bedzhov I. 2010. A Cdh1(HA) knock-in allele rescues the Cdh1(-/-) phenotype but shows essential Cdh1 function during placentation. Dev Dyn :. [PubMed: 20652949]  [MGI Ref ID J:163273]

Stephenson RO; Yamanaka Y; Rossant J. 2010. Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin. Development 137(20):3383-91. [PubMed: 20826529]  [MGI Ref ID J:165812]

Tinkle CL; Lechler T; Pasolli HA; Fuchs E. 2004. Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses. Proc Natl Acad Sci U S A 101(2):552-7. [PubMed: 14704278]  [MGI Ref ID J:87432]

Tinkle CL; Pasolli HA; Stokes N; Fuchs E. 2008. New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc Natl Acad Sci U S A 105(40):15405-10. [PubMed: 18809908]  [MGI Ref ID J:144091]

Tunggal JA; Helfrich I; Schmitz A; Schwarz H; Gunzel D; Fromm M; Kemler R; Krieg T; Niessen CM. 2005. E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions. EMBO J 24(6):1146-56. [PubMed: 15775979]  [MGI Ref ID J:97223]

Young P; Boussadia O; Berger P; Leone DP; Charnay P; Kemler R; Suter U. 2002. E-cadherin is required for the correct formation of autotypic adherens junctions of the outer mesaxon but not for the integrity of myelinated fibers of peripheral nerves. Mol Cell Neurosci 21(2):341-51. [PubMed: 12401452]  [MGI Ref ID J:80099]

Young P; Boussadia O; Halfter H; Grose R; Berger P; Leone DP; Robenek H; Charnay P; Kemler R; Suter U. 2003. E-cadherin controls adherens junctions in the epidermis and the renewal of hair follicles. EMBO J 22(21):5723-33. [PubMed: 14592971]  [MGI Ref ID J:86510]

de Vries WN; Evsikov AV; Haac BE; Fancher KS; Holbrook AE; Kemler R; Solter D; Knowles BB. 2004. Maternal beta-catenin and E-cadherin in mouse development. Development 131(18):4435-45. [PubMed: 15306566]  [MGI Ref ID J:96042]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain is maintained as a homozygote.
Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	See control note:	C57BL/6J mice (Stock No. 000664) may be used as controls.
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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