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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N10+N1F7 (27-APR-08) Donating Investigator RolfK Kemler, Max Planck Institute for Immunobiology Description
These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted into intron 10 of the targeted gene, and another loxP site was inserted into intron 5. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and then backcrossed to the same for 10 generations before being made homozygous.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cdh1
002473 B6;129S2-Cdh1tm1Kem/J View Strains carrying other alleles of Cdh1 (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cdh1tm2Kem relatedResearch Tools
Cre-lox System (loxP-flanked Sequences)
Cell Biology Research
Defects in Cell Adhesion Molecules
Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Cdh1tm2Kem | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rolf Kemler | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | E-cadlox; E-cadherinflox; E-cadherinfloxed; Ecadfl; | ||
| Mutation Made By | Wilhelmine de Vries, The Jackson Laboratory | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Cdh1, cadherin 1 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | AA960649; Arc-1; CD324; CDHE; E-cadherin; ECAD; Ecad; L-CAM; LCAM; UM; UVO; Um; expressed sequence AA960649; uvomorulin; | ||
| Molecular Note | Single loxP sites flanked a region encompassing exons 6 through 10 was after a floxed selection cassette was excised via in vitro cre mediated recombination. [MGI Ref ID J:77170] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cdh1tm2Kem, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Boussadia O; Kutsch S; Hierholzer A; Delmas V; Kemler R. 2002. E-cadherin is a survival factor for the lactating mouse mammary gland. Mech Dev 115(1-2):53-62. [PubMed: 12049767] [MGI Ref ID J:77170]
Additional References
Cdh1tm2Kem relatedBattle MA; Konopka G; Parviz F; Gaggl AL; Yang C; Sladek FM; Duncan SA. 2006. Hepatocyte nuclear factor 4alpha orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver. Proc Natl Acad Sci U S A 103(22):8419-24. [PubMed: 16714383] [MGI Ref ID J:110205]
Cali G; Zannini M; Rubini P; Tacchetti C; D'Andrea B; Affuso A; Wintermantel T; Boussadia O; Terracciano D; Silberschmidt D; Amendola E; De Felice M; Schutz G; Kemler R; Di Lauro R; Nitsch L. 2007. Conditional inactivation of the E-cadherin gene in thyroid follicular cells affects gland development but does not impair junction formation. Endocrinology 148(6):2737-46. [PubMed: 17347311] [MGI Ref ID J:122323]
Ceteci F; Ceteci S; Karreman C; Kramer BW; Asan E; Gotz R; Rapp UR. 2007. Disruption of Tumor Cell Adhesion Promotes Angiogenic Switch and Progression to Micrometastasis in RAF-Driven Murine Lung Cancer. Cancer Cell 12(2):145-159. [PubMed: 17692806] [MGI Ref ID J:124321]
Kan NG; Stemmler MP; Junghans D; Kanzler B; de Vries WN; Dominis M; Kemler R. 2007. Gene replacement reveals a specific role for E-cadherin in the formation of a functional trophectoderm. Development 134(1):31-41. [PubMed: 17138661] [MGI Ref ID J:117056]
Tinkle CL; Lechler T; Pasolli HA; Fuchs E. 2004. Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses. Proc Natl Acad Sci U S A 101(2):552-7. [PubMed: 14704278] [MGI Ref ID J:87432]
Tunggal JA; Helfrich I; Schmitz A; Schwarz H; Gunzel D; Fromm M; Kemler R; Krieg T; Niessen CM. 2005. E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions. EMBO J 24(6):1146-56. [PubMed: 15775979] [MGI Ref ID J:97223]
Young P; Boussadia O; Berger P; Leone DP; Charnay P; Kemler R; Suter U. 2002. E-cadherin is required for the correct formation of autotypic adherens junctions of the outer mesaxon but not for the integrity of myelinated fibers of peripheral nerves. Mol Cell Neurosci 21(2):341-51. [PubMed: 12401452] [MGI Ref ID J:80099]
Young P; Boussadia O; Halfter H; Grose R; Berger P; Leone DP; Robenek H; Charnay P; Kemler R; Suter U. 2003. E-cadherin controls adherens junctions in the epidermis and the renewal of hair follicles. EMBO J 22(21):5723-33. [PubMed: 14592971] [MGI Ref ID J:86510]
de Vries WN; Evsikov AV; Haac BE; Fancher KS; Holbrook AE; Kemler R; Solter D; Knowles BB. 2004. Maternal beta-catenin and E-cadherin in mouse development. Development 131(18):4435-45. [PubMed: 15306566] [MGI Ref ID J:96042]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is maintained as a homozygote. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Homozygous for Cdh1tm2Kem *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.20 Homozygous for Cdh1tm2Kem x Homozygous for Cdh1tm2Kem
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Homozygous for Cdh1tm2Kem *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.60 Homozygous for Cdh1tm2Kem x Homozygous for Cdh1tm2Kem
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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