Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N10+N2F2 (06-DEC-11)
Generation DefinitionsDonating Investigator Prof. Dr. RolfK Kemler, Max Planck Institute for Immunobiology Description
These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted into intron 10 of the targeted gene, and another loxP site was inserted into intron 5. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and then backcrossed to the same for 10 generations before being made homozygous.
| Control | ||
|---|---|---|
| See control note: | C57BL/6J mice (Stock No. 000664) may be used as controls. | |
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Cdh1
016933 B6.129P2(Cg)-Cdh1tm1Cle/J 002473 B6;129S2-Cdh1tm1Kem/J View Strains carrying other alleles of Cdh1 (2 strains)
Introduction to Cre-lox technology
View Related Disease (OMIM) Terms
Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Breast Cancer (CDH1)
Endometrial Cancer (CDH1)
Gastric Cancer, Hereditary Diffuse; HDGC (CDH1)
Ovarian Cancer (CDH1)
Prostate Cancer (CDH1)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Cdh1tm2Kem relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
Cell Biology Research
Defects in Cell Adhesion Molecules
Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
| Allele Symbol | Cdh1tm2Kem | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rolf Kemler | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | E-cadlox; E-cadherinflox; E-cadherinfloxed; Ecadfl; | ||
| Mutation Made By | Wilhelmine de Vries, The Jackson Laboratory | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Cdh1, cadherin 1 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | AA960649; Arc-1; CD324; CDHE; E-cad; E-cadherin; ECAD; Ecad; L-CAM; LCAM; UM; UVO; Um; expressed sequence AA960649; uvomorulin; | ||
| Molecular Note | Single loxP sites flanked a region encompassing exons 6 through 10 followed by a floxed selection cassette was excised via in vitro cre mediated recombination. [MGI Ref ID J:77170] | ||
Genotyping Protocols
Cdh1tm2Kem, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Boussadia O; Kutsch S; Hierholzer A; Delmas V; Kemler R. 2002. E-cadherin is a survival factor for the lactating mouse mammary gland. Mech Dev 115(1-2):53-62. [PubMed: 12049767] [MGI Ref ID J:77170]
Cdh1tm2Kem relatedBattle MA; Konopka G; Parviz F; Gaggl AL; Yang C; Sladek FM; Duncan SA. 2006. Hepatocyte nuclear factor 4alpha orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver. Proc Natl Acad Sci U S A 103(22):8419-24. [PubMed: 16714383] [MGI Ref ID J:110205]
Cali G; Zannini M; Rubini P; Tacchetti C; D'Andrea B; Affuso A; Wintermantel T; Boussadia O; Terracciano D; Silberschmidt D; Amendola E; De Felice M; Schutz G; Kemler R; Di Lauro R; Nitsch L. 2007. Conditional inactivation of the E-cadherin gene in thyroid follicular cells affects gland development but does not impair junction formation. Endocrinology 148(6):2737-46. [PubMed: 17347311] [MGI Ref ID J:122323]
Ceteci F; Ceteci S; Karreman C; Kramer BW; Asan E; Gotz R; Rapp UR. 2007. Disruption of Tumor Cell Adhesion Promotes Angiogenic Switch and Progression to Micrometastasis in RAF-Driven Murine Lung Cancer. Cancer Cell 12(2):145-159. [PubMed: 17692806] [MGI Ref ID J:124321]
Chauhan BK; Disanza A; Choi SY; Faber SC; Lou M; Beggs HE; Scita G; Zheng Y; Lang RA. 2009. Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination. Development 136(21):3657-67. [PubMed: 19820184] [MGI Ref ID J:153957]
Damsky WE; Curley DP; Santhanakrishnan M; Rosenbaum LE; Platt JT; Gould Rothberg BE; Taketo MM; Dankort D; Rimm DL; McMahon M; Bosenberg M. 2011. beta-Catenin Signaling Controls Metastasis in Braf-Activated Pten-Deficient Melanomas. Cancer Cell 20(6):741-54. [PubMed: 22172720] [MGI Ref ID J:178598]
Fiederling A; Ewert R; Andreyeva A; Jungling K; Gottmann K. 2011. E-cadherin is required at GABAergic synapses in cultured cortical neurons. Neurosci Lett 501(3):167-72. [PubMed: 21782891] [MGI Ref ID J:175782]
Hofmann M; Pircher H. 2011. E-cadherin promotes accumulation of a unique memory CD8 T-cell population in murine salivary glands. Proc Natl Acad Sci U S A 108(40):16741-6. [PubMed: 21930933] [MGI Ref ID J:177361]
Hwang S; Zimmerman NP; Agle KA; Turner JR; Kumar SN; Dwinell MB. 2012. E-cadherin is critical for collective sheet migration and is regulated by the chemokine CXCL12 protein during restitution. J Biol Chem 287(26):22227-40. [PubMed: 22549778] [MGI Ref ID J:187535]
Kan NG; Stemmler MP; Junghans D; Kanzler B; de Vries WN; Dominis M; Kemler R. 2007. Gene replacement reveals a specific role for E-cadherin in the formation of a functional trophectoderm. Development 134(1):31-41. [PubMed: 17138661] [MGI Ref ID J:117056]
Kanatsu-Shinohara M; Takehashi M; Takashima S; Lee J; Morimoto H; Chuma S; Raducanu A; Nakatsuji N; Fassler R; Shinohara T. 2008. Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. Cell Stem Cell 3(5):533-42. [PubMed: 18983968] [MGI Ref ID J:149789]
Karpowicz P; Willaime-Morawek S; Balenci L; DeVeale B; Inoue T; van der Kooy D. 2009. E-Cadherin regulates neural stem cell self-renewal. J Neurosci 29(12):3885-96. [PubMed: 19321785] [MGI Ref ID J:147274]
Li CY; Cha W; Luder HU; Charles RP; McMahon M; Mitsiadis TA; Klein OD. 2012. E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor. Dev Biol 366(2):357-66. [PubMed: 22537490] [MGI Ref ID J:185423]
Libusova L; Stemmler MP; Hierholzer A; Schwarz H; Kemler R. 2010. N-cadherin can structurally substitute for E-cadherin during intestinal development but leads to polyp formation. Development 137(14):2297-305. [PubMed: 20534673] [MGI Ref ID J:161852]
Pontoriero GF; Smith AN; Miller LA; Radice GL; West-Mays JA; Lang RA. 2009. Co-operative roles for E-cadherin and N-cadherin during lens vesicle separation and lens epithelial cell survival. Dev Biol 326(2):403-17. [PubMed: 18996109] [MGI Ref ID J:145191]
Reardon SN; King ML; MacLean JA 2nd; Mann JL; DeMayo FJ; Lydon JP; Hayashi K. 2012. CDH1 is essential for endometrial differentiation, gland development, and adult function in the mouse uterus. Biol Reprod 86(5):141, 1-10. [PubMed: 22378759] [MGI Ref ID J:185830]
Schneider MR; Dahlhoff M; Horst D; Hirschi B; Trulzsch K; Muller-Hocker J; Vogelmann R; Allgauer M; Gerhard M; Steininger S; Wolf E; Kolligs FT. 2010. A key role for E-cadherin in intestinal homeostasis and Paneth cell maturation. PLoS One 5(12):e14325. [PubMed: 21179475] [MGI Ref ID J:168992]
Stemmler MP; Bedzhov I. 2010. A Cdh1(HA) knock-in allele rescues the Cdh1(-/-) phenotype but shows essential Cdh1 function during placentation. Dev Dyn :. [PubMed: 20652949] [MGI Ref ID J:163273]
Stephenson RO; Yamanaka Y; Rossant J. 2010. Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin. Development 137(20):3383-91. [PubMed: 20826529] [MGI Ref ID J:165812]
Tinkle CL; Lechler T; Pasolli HA; Fuchs E. 2004. Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses. Proc Natl Acad Sci U S A 101(2):552-7. [PubMed: 14704278] [MGI Ref ID J:87432]
Tinkle CL; Pasolli HA; Stokes N; Fuchs E. 2008. New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc Natl Acad Sci U S A 105(40):15405-10. [PubMed: 18809908] [MGI Ref ID J:144091]
Tunggal JA; Helfrich I; Schmitz A; Schwarz H; Gunzel D; Fromm M; Kemler R; Krieg T; Niessen CM. 2005. E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions. EMBO J 24(6):1146-56. [PubMed: 15775979] [MGI Ref ID J:97223]
Young P; Boussadia O; Berger P; Leone DP; Charnay P; Kemler R; Suter U. 2002. E-cadherin is required for the correct formation of autotypic adherens junctions of the outer mesaxon but not for the integrity of myelinated fibers of peripheral nerves. Mol Cell Neurosci 21(2):341-51. [PubMed: 12401452] [MGI Ref ID J:80099]
Young P; Boussadia O; Halfter H; Grose R; Berger P; Leone DP; Robenek H; Charnay P; Kemler R; Suter U. 2003. E-cadherin controls adherens junctions in the epidermis and the renewal of hair follicles. EMBO J 22(21):5723-33. [PubMed: 14592971] [MGI Ref ID J:86510]
de Vries WN; Evsikov AV; Haac BE; Fancher KS; Holbrook AE; Kemler R; Solter D; Knowles BB. 2004. Maternal beta-catenin and E-cadherin in mouse development. Development 131(18):4435-45. [PubMed: 15306566] [MGI Ref ID J:96042]
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is maintained as a homozygote. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
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Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Cdh1tm2Kem
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Cdh1tm2Kem x Homozygous for Cdh1tm2Kem Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
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Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Cdh1tm2Kem
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Cdh1tm2Kem x Homozygous for Cdh1tm2Kem Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Control | ||
|---|---|---|
| See control note: | C57BL/6J mice (Stock No. 000664) may be used as controls. | |
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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