Description

Strain Information

Type Mutant Stock; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Ovarian Transplant-Cross-Intercross         (Female x Male) homozygous ovarian transplant host x +/? sibling then intercross of the heterozygous offspring Species laboratory mouse Generation F30 (06-DEC-07)

Important Note
This strain is homozygous for the Pemt^tm1J targeted mutation

Related Strains

Strains carrying   Pemt^tm1J allele

003187

B6;129P2-Pemttm1J/J

View Strains carrying   Pemt^tm1J     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Pemt^tm1J/Pemt^tm1J tnyw/tnyw

        B6;129P2 Pemt^tm1J-tnyw/J

• nervous system phenotype
• brain vacuoles (MGI Ref ID J:125079)
  • tiny wasting homozygotes assessed at 16 days of age to 3.5 weeks of age display vacuoles in the brain
• growth/size phenotype
• decreased body size (MGI Ref ID J:125079)
  • all tiny wasting homozygotes are smaller than heterozygous controls and can be identified by 2 weeks of age by their smaller wasting bodies
• behavior/neurological phenotype
• abnormal locomotor coordination (MGI Ref ID J:125079)
  • some tiny wasting homozygotes display abnormal locomotion
• impaired limb coordination (MGI Ref ID J:125079)
• impaired righting response (MGI Ref ID J:125079)
  • some tiny wasting homozygotes are unable to right themselves
• lethality-postnatal
• postnatal lethality (MGI Ref ID J:125079)
  • most tiny wasting homozygotes die by 3 weeks of age
• life span-post-weaning/aging
• premature death (MGI Ref ID J:125079)
• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:125079)
  • ABR assessment on 2 tiny wasting homozygotes failed to detect any abnormality
• endocrine/exocrine gland phenotype
• *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:125079)
  • histology of pituitary glands, thyroid glands, and somatic organs reveals no abnormalities

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Pemt^tm1J related

Cancer Research
Genes Regulating Growth and Proliferation

Cardiovascular Research
Hypocholesterolemia
Hypotriglyceridemia

Metabolism Research

tnyw related

Developmental Biology Research
Growth Defects
Postnatal Mortality

Genes & Alleles

Gene & Allele Information

Allele Symbol		Pemttm1J
Allele Name		targeted mutation 1, Jackson
Allele Type		Targeted (knock-out)
Common Name(s)		Pempt2-; Pemt-;
Allele Symbol		tnyw
Allele Name		tiny wasting
Allele Type		Spontaneous
Strain of Origin		B6;129P2-Pemttm1J/J

Genotyping

Genotyping Information

Genotyping Protocols

Pemt^tm1J, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

tnyw related

Marden C; Ward-Bailey PF; Donahue LR; Bronson RT; Johnson KR. 2007. Tiny wasting (tnyw); a new autosomal recessive mutation on Chromosome 3 MGI Direct Data Submission :.  [MGI Ref ID J:125079]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Mating System	Ovarian Transplant-Cross-Intercross         (Female x Male)
	homozygous ovarian transplant host x +/? sibling then intercross of the heterozygous offspring

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection.
Important Note	This strain is homozygous for the Pemttm1J targeted mutation

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.