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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain NOD/ShiLt H2 Haplotype g7 Generation N1F15pN1
Generation DefinitionsDonating Investigator Edward Leiter, The Jackson Laboratory Appearance
albino
Related Genotype: A/A Tyrc/TyrcDescription
The donating investigator reports that hemizygous transgenic mice are viable, fertile and normal in size. FACS analysis of splenic lymphocytes shows transgenic expression in 36% of CD4+ and 6% of CD8+ T cells and minimal expression (background levels) in B cells and macrophages. When compared to wild-type NOD/ShiLt mice, the diabetes incidence is lower (50%) and diabetes onset is delayed. Adoptive transfer experiments with splenocytes and bone marrow from hemizygous CD4-DsRed transgenic mice does not confer diabetes protection on NOD inbred mice, suggesting that the agent of protection is not dependent on hematopoietic cells. No homozygous transgenic mice have been identified in litters produced from hemizygote intercrosses, suggesting that homozygotes are not viable.This strain is useful for tracking resting and activated T cells in vivo and in vitro.
Development
A transgenic construct containing the Red Florescent Protein, DsRed, cDNA under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 4 was mated to NOD/ShiLt prior to sibling mating. In 2007, the T1DR received this transgenic strain at N1F15 and mated to Stock No. 001976-NOD/ShiLtJ once prior to sibling mating.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
002447 B10.129S2(B6)-Cd4tm1Litt/J 010514 B10.Cg-H2g Tg(Cd4-Klra1)6295Dl/J 002663 B6.129S2-Cd4tm1Mak/J 002269 B6.129S6-Cd4tm1Knw/J 002664 B6;129S-Cd4tm1Mak Cd8atm1Mak/J 002268 B6;129S6-Cd4tm1Knw/J 012895 C57BL/6-Tg(Cd4-Il17rb)5Cdon/J 014639 C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J 014644 C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J 006483 CBy.129S2(B6)-Cd4tm1Mak/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Cd4-DsRed)4Lt/0
NOD/ShiLt-Tg(Cd4-DsRed)4Lt
- immune system phenotype
- decreased susceptibility to autoimmune diabetes
- diabetes incidence (50%) is lower than that displayed by control NOD/ShiLt animals; diabetes onset is delayed relative to control NOD/ShiLt (MGI Ref ID J:127785)
Tg(Cd4-DsRed)4Lt/Tg(Cd4-DsRed)4Lt
NOD/ShiLt-Tg(Cd4-DsRed)4Lt
- mortality/aging
- complete preweaning lethality
- live homozygous animals have not been identified (MGI Ref ID J:127785)
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD Transgenics
Immunology and Inflammation Research
Autoimmunity
Type 1 Diabetes
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cd4-DsRed)4Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Edward Leiter | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | CD4-DsRed Line 4; Cd4-RFP; | ||
| Mutation Made By | Edward Leiter, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Site of Expression | FACs analysis has detected expression of the transgene in 36% of CD4 and 6% of CD8 T-cells with background levels in splenic B cells and macrophages. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | Cd4, CD4 antigen, mouse, laboratory | ||
| Molecular Note | The construct has the DsRed gene under control of the Cd4 antigen promoter. FACS analysis of splenic lymphocytes shows fluorescence in 36% of CD4 and 6% of CD8 T-cells with minimal expression (only background levels) in splenic B cells or macrophages. [MGI Ref ID J:127785] | ||
| Gene Symbol and Name | Tg(Cd4-DsRed)4Lt, transgene insertion 4, Edward Leiter | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CD4-DsRed Line 4; Cd4-RFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic DsRed, Melt Curve Analysis
Generic DsRed, QPCR
Generic DsRed, Separated PCR
Generic DsRed, Standard PCR
Tg(DsRed), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Leiter E. 2007. Flourescent tracking of NOD derived T cells in vivo and in vitro MGI Direct Data Submission :. [MGI Ref ID J:127785]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
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Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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