Strain Name: |
NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J |
|---|---|
Stock Number: |
005328 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Cd4; DsRed; Tg(Cd4-DsRed)4Lt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain NOD/ShiLt Donating Investigator Edward Leiter, The Jackson Laboratory H2 Haplotype g7 Generation N1F15+N1 (06-MAR-08) Appearance
albino
Related Genotype: A/A Tyrc/TyrcStrain Description
The donating investigator reports that hemizygous transgenic mice are viable, fertile and normal in size. FACS analysis of splenic lymphocytes shows transgenic expression in 36% of CD4+ and 6% of CD8+ T-cells and minimal expression (background levels) in B cells and macrophages. When compared to wild-type NOD/ShiLt mice, the diabetes incidence is lower (50%) and diabetes onset is delayed. Adoptive transfer experiments with splenocytes and bone marrow from hemizygous CD4-DsRed transgenic mice does not confer diabetes protection on NOD inbred mice, suggesting that the agent of protection is not dependent on hematopoietic cells. No homozygous transgenic mice have been identified in litters produced from hemizygote intercrosses, suggesting that homozygotes are not viable.This strain is useful for tracking resting and activated T cells in vivo and in vitro.
Strain Development
A transgenic construct containing the Red Florescent Protein, DsRed, cDNA under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 4 was mated to NOD/ShiLt prior to sibling mating. In 2007, the T1DR received this transgenic strain at N1F15 and mated to Stock No. 001976-NOD/ShiLtJ once prior to sibling mating.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Cd4-DsRed)4Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Edward Leiter | ||
| Common Name(s) | CD4-DsRed Line 4; Cd4-RFP; | ||
| Mutation Made By | Edward Leiter, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Site of Expression | FACs analysis has detected expression of the transgene in 36% of CD4 and 6% of CD8 T-cells with background levels in splenic B cells and macrophages. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | Cd4, CD4 antigen, mouse, laboratory | ||
| Molecular Note | The construct has the DsRed gene under control of the Cd4 antigen promoter. FACS analysis of splenic lymphocytes shows fluorescence in 36% of CD4 and 6% of CD8 T-cells with minimal expression (only background levels) in splenic B cells or macrophages. [MGI Ref ID J:127785] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Tg(DsRed)
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
002447 B10.129S2(B6)-Cd4tm1Litt/J 002663 B6.129S2-Cd4tm1Mak/J 002269 B6.129S6-Cd4tm1Knw/J 002664 B6;129S-Cd4tm1Mak Cd8atm1Mak/J 002268 B6;129S6-Cd4tm1Knw/J 006483 CBy.129S2(B6)-Cd4tm1Mak/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
DsRed relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Leiter E. 2007. Flourescent tracking of NOD derived T cells in vivo and in vitro MGI Direct Data Submission :. [MGI Ref ID J:127785]
Price and Supply Information
| Strain Name: | NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J |
| Stock Number: | 005328 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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