Strain Name: |
NOD.Cg-Il10tm1Cgn Casp1tm1Sesh/LtJ |
|---|---|
Stock Number: |
005346 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | NOD.IL10,Casp1; |
| Genes & Alleles | Casp1; Casp1tm1Sesh; Il10; Il10tm1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain NOD/ShiLt Donor Strain 129 Donating Investigator Edward Leiter, The Jackson Laboratory H2 Haplotype g7 Appearance
albino, pink-eyed
Related Genotype: A/? Tyrc/TyrcStrain Description
Mice homozygous for the Il10 and Casp1 targeted mutations are viable and fertile when housed under SPF conditions. NOD/Lt mice deficient for both of these genes develop type 1 diabetes at a rate equivalent to the parental strains. The Il10 targeted mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when maintained under standard housing conditions.Strain Development
For details related to the construction of each allele, please refer to the entries for strains 004947 and 004266. NOD.Il10 homozygous mice were crossed to NOD.Casp1 homozygous mice prior to making homozygous for both alleles. In 2006, the T1DR received double homozygous mice at generation F16.
Gene & Allele Details
| Allele Symbol | Casp1tm1Sesh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tara Seshadri | ||
| Common Name(s) | Casp1 -; Caspase-1-; ICE -; casp-1-; | ||
| Mutation Made By | Edward Leiter, The Jackson Laboratory | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Casp1, caspase 1 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | Caspase-1; ICE; IL1BC; Il1bc; P45; interleukin 1 beta convertase; interleukin 1 beta-converting enzyme; | ||
| Molecular Note | A neomycin expression cassette was inserted into exon 6, deleting 31 bp of sequence encoding the region of the active site and rendering the sequence out of frame after the insertion. Northern blot analysis on spleen RNA demonstrated an absence of the normal transcript in homozygous mice, and western blot analysis showed that the protein was not expressed in peritoneal macrophages of homozygous mice. [MGI Ref ID J:22964] | ||
| Allele Symbol | Il10tm1Cgn | ||
| Allele Name | targeted mutation 1, University of Cologne | ||
| Common Name(s) | IL-10 KO; IL-10-; IL-10KO; IL-10KO; Il10-; | ||
| Mutation Made By | Ralf Kuhn, University of Cologne | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14.1 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Il10, interleukin 10 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CSIF; IL-10; IL10A; IL10X; Il-10; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; | ||
| Molecular Note | A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable in ELISA assays in supernatants of in vitro splenic T cells derived from homozygous mice. | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Casp1tm1Sesh
Il10tm1Cgn
Related Strains
Strains carrying Casp1tm1Sesh allele
004947 NOD.129S2(B6)-Casp1tm1Sesh/LtJ View Strains carrying Casp1tm1Sesh (1 strain)
004368 129(B6)-Il10tm1Cgn/J 002250 B10.129P2(B6)-Il10tm1Cgn/J 002251 B6.129P2-Il10tm1Cgn/J 005912 B6.Cg-Il10tm1Cgn (D3Mit49-D3Mit348)/Lt 004333 C.129P2(B6)-Il10tm1Cgn/J 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 004228 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/Dvs 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ 004266 NOD.Cg-Il10tm1Cgn/DvsJ
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Casp1tm1Sesh relatedCancer Research
Growth Factors/Receptors/Cytokines
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (Congenics with mutations affecting cytokine production by autoreactive T cells)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Cytokine Production by Autoreactive T Cells)
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines (control strain)
Growth Factors/Receptors/Cytokines
Internal/Organ Research
Gastrointestinal Defects (colitis)
Il10tm1Cgn relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM)
Immunology and Inflammation Research
Immunodeficiency (B cell defects)
Research Tools
Diabetes and Obesity Research
Cancer Research
Growth Factors/Receptors/Cytokines
Hematological Research
Anemia, Iron Deficiency and Transport Defects (hemolytic)
Immunological Defects
Immunology and Inflammation Research
Autoimmunity
Growth Factors/Receptors/Cytokines
Immunodeficiency
Inflammation (Inflammatory bowel disease)
References
Additional ReferencesPrice and Supply Information
| Strain Name: | NOD.Cg-Il10tm1Cgn Casp1tm1Sesh/LtJ |
| Stock Number: | 005346 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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