Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD/ShiLt Donor Strain 129S4/SvJae and 129P2/OlaHsd (via C57BL/6) H2 Haplotype g7 Generation N1F5p

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c

Development
This triple mutant was created by intercrossing NOD.129S4(B6)-Art2a^tm1Fkn Art2b^tm1Fkn/Lt and NOD.129P2(B6)-Cd38^tm1Lnd/LtJ (Stock No. 004311). F1 mice were backcrossed to NOD.129S4(B6)-Art2a^tm1Fkn Art2b^tm1Fkn/Lt to generate mice homozygous for the Art2a and Art2b alleles and heterozygous for Cd38^tm1Lnd. Subsequent matings generated the triple homozygous mutant.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Art2a^tm1Fkn allele

004307	NOD.129S4(B6)-Art2atm1Fkn Art2btm1Fkn/Lt
008252	NOD.129S4(B6)-Art2atm1Fkn Art2btm1Fkn/LtJ

View Strains carrying   Art2a^tm1Fkn     (2 strains)

Strains carrying   Art2b^tm1Fkn allele

004307	NOD.129S4(B6)-Art2atm1Fkn Art2btm1Fkn/Lt
008252	NOD.129S4(B6)-Art2atm1Fkn Art2btm1Fkn/LtJ

View Strains carrying   Art2b^tm1Fkn     (2 strains)

Strains carrying   Cd38^tm1Lnd allele

003727	B6.129P2-Cd38tm1Lnd/J
004311	NOD.129P2(B6)-Cd38tm1Lnd/LtJ
005345	NOD.Cg-Cd38tm1Lnd Prkdcscid/LtJ

View Strains carrying   Cd38^tm1Lnd     (3 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Art2a^tm1Fkn/Art2a^tm1Fkn Art2b^tm1Fkn/Art2b^tm1Fkn Cd38^tm1Lnd/Cd38^tm1Lnd

        NOD.129(B6)-Cd38^tm1Lnd Art2a^tm1Fkn Art2b^tm1Fkn/Lt

• immune system phenotype
• decreased susceptibility to autoimmune diabetes (MGI Ref ID J:108097)
  • double knockout NOD mice exhibit strong protection from diabetes in both sexes; female knockouts acquire diabetes at a frequency of 25% by 28 weeks compared to 100% in Cd38-null NOD females; double knockout NOD males are completely protected at 28 weeks compared to 100% incidence in Cd38-null NOD males

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Cd38^tm1Lnd related

Cancer Research
Genes Regulating Growth and Proliferation

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Art2atm1Fkn
Allele Name		targeted mutation 1, Friedrich Koch-Noltet
Allele Type		Targeted (knock-out)
Common Name(s)		ART2.1-;
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Art2a, ADP-ribosyltransferase 2a
Chromosome		7
Gene Common Name(s)		ART1P; Ly92a; RT6; Rt-6; Rt6; Rt6-1; rat homolog Rt6, locus 1; rat homolog of transplantable antigen gene RT6;
General Note		Successfully targeted ES cells were subsequently targeted with the construct for the Art2b allele.
Molecular Note		The coding region was disrupted by a neomycin selection cassette inserted by homologous recombination. ES cell were then transfected with the Art2btm1Fkn construct. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes. [MGI Ref ID J:79607]
Allele Symbol		Art2btm1Fkn
Allele Name		targeted mutation 1, Friedrich Koch-Nolte
Allele Type		Targeted (knock-out)
Common Name(s)		ART2.2-;
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Art2b, ADP-ribosyltransferase 2b
Chromosome		7
Gene Common Name(s)		ART2.2; Ag-F; Art2; Art2a; LY2; Ly-2; Ly92b; MGC116041; PtaA; RT6.1; RT6.2; Rt-6; Rt6; Rt6-2; homolog of rat transplantable antigen gene RT6; rat homolog Rt6, locus 2;
General Note		The targeting vector was electroporated into J1 ES cells carrying Art2atm1Fkn.
Molecular Note		The coding region was disrupted by a hygromycin selection cassette inserted by homologous recombination. This construct was used to transfect mice in which the Art2atm1Fkn allele had already been created Transcript was undetected in homozygousmutant mice by RT-PCR analysis of T cells isolated from lymph nodes. FACS analysis of thymocytes and lymphocytes indicated a lack of protein expression on the cell surface. [MGI Ref ID J:79607]
Allele Symbol		Cd38tm1Lnd
Allele Name		targeted mutation 1, Frances E Lund
Allele Type		Targeted (knock-out)
Common Name(s)		CD38-; CD38null;
Mutation Made By		Debra Cockayne,   Roche Bioscience
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14.1
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Cd38, CD38 antigen
Chromosome		5
Gene Common Name(s)		CD38 antigen, related sequence 1; Cd38-rs1; T10;
Molecular Note		A neomycin selection cassette replaced a genomic fragment containing exons 2 and 3, which encode the putative active site of the encoded protein. Homozygous mice lacked transcripts derived from this allele (data not shown). Flow cytometry analysis on splenocytes derived from homozygous mice confirmed that no detectable protein was expressed on the cell surface. [MGI Ref ID J:49170]

Genotyping

Genotyping Information

Genotyping Protocols

Cd38^tm1Ldn, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chen J; Chen YG; Reifsnyder PC; Schott WH; Lee CH; Osborne M; Scheuplein F; Haag F; Koch-Nolte F; Serreze DV; Leiter EH. 2006. Targeted disruption of CD38 accelerates autoimmune diabetes in NOD/Lt mice by enhancing autoimmunity in an ADP-ribosyltransferase 2-dependent fashion. J Immunol 176(8):4590-9. [PubMed: 16585549]  [MGI Ref ID J:108097]

Chen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929]  [MGI Ref ID J:112615]

Additional References

Art2a^tm1Fkn related

Kawamura H; Aswad F; Minagawa M; Govindarajan S; Dennert G. 2006. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol 176(4):2152-60. [PubMed: 16455971]  [MGI Ref ID J:129123]

Krebs C; Adriouch S; Braasch F; Koestner W; Leiter EH; Seman M; Lund FE; Oppenheimer N; Haag F; Koch-Nolte F. 2005. CD38 controls ADP-ribosyltransferase-2-catalyzed ADP-ribosylation of T cell surface proteins. J Immunol 174(6):3298-305. [PubMed: 15749861]  [MGI Ref ID J:97699]

Ohlrogge W; Haag F; Lohler J; Seman M; Littman DR; Killeen N; Koch-Nolte F. 2002. Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol 22(21):7535-42. [PubMed: 12370300]  [MGI Ref ID J:79607]

Art2b^tm1Fkn related

Heiss K; Janner N; Mahnss B; Schumacher V; Koch-Nolte F; Haag F; Mittrucker HW. 2008. High sensitivity of intestinal CD8+ T cells to nucleotides indicates P2X7 as a regulator for intestinal T cell responses. J Immunol 181(6):3861-9. [PubMed: 18768840]  [MGI Ref ID J:139109]

Kawamura H; Aswad F; Minagawa M; Govindarajan S; Dennert G. 2006. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol 176(4):2152-60. [PubMed: 16455971]  [MGI Ref ID J:129123]

Krebs C; Adriouch S; Braasch F; Koestner W; Leiter EH; Seman M; Lund FE; Oppenheimer N; Haag F; Koch-Nolte F. 2005. CD38 controls ADP-ribosyltransferase-2-catalyzed ADP-ribosylation of T cell surface proteins. J Immunol 174(6):3298-305. [PubMed: 15749861]  [MGI Ref ID J:97699]

Ohlrogge W; Haag F; Lohler J; Seman M; Littman DR; Killeen N; Koch-Nolte F. 2002. Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol 22(21):7535-42. [PubMed: 12370300]  [MGI Ref ID J:79607]

Cd38^tm1Lnd related

Aksoy P; White TA; Thompson M; Chini EN. 2006. Regulation of intracellular levels of NAD: A novel role for CD38. Biochem Biophys Res Commun 345(4):1386-92. [PubMed: 16730329]  [MGI Ref ID J:109655]

Barbosa MT; Soares SM; Novak CM; Sinclair D; Levine JA; Aksoy P; Chini EN. 2007. The enzyme CD38 (a NAD glycohydrolase, EC 3.2.2.5) is necessary for the development of diet-induced obesity. FASEB J 21(13):3629-39. [PubMed: 17585054]  [MGI Ref ID J:134922]

Bergthorsdottir S; Gallagher A; Jainandunsing S; Cockayne D; Sutton J; Leanderson T; Gray D. 2001. Signals that initiate somatic hypermutation of B cells in vitro. J Immunol 166(4):2228-34. [PubMed: 11160276]  [MGI Ref ID J:127146]

Ceni C; Pochon N; Villaz M; Muller-Steffner H; Schuber F; Baratier J; De Waard M; Ronjat M; Moutin MJ. 2006. The CD38-independent ADP-ribosyl cyclase from mouse brain synaptosomes: a comparative study of neonate and adult brain. Biochem J 395(2):417-26. [PubMed: 16411897]  [MGI Ref ID J:115899]

Cockayne DA; Muchamuel T; Grimaldi JC; Muller-Steffner H; Randall TD; Lund FE; Murray R; Schuber F; Howard MC. 1998. Mice deficient for the ecto-nicotinamide adenine dinucleotide Blood 92(4):1324-33. [PubMed: 9694721]  [MGI Ref ID J:49170]

Deshpande DA; White TA; Guedes AG; Milla C; Walseth TF; Lund FE; Kannan MS. 2005. Altered airway responsiveness in CD38-deficient mice. Am J Respir Cell Mol Biol 32(2):149-56. [PubMed: 15557017]  [MGI Ref ID J:107616]

Gul R; Kim SY; Park KH; Kim BJ; Kim SJ; Im MJ; Kim UH. 2008. A novel signaling pathway of ADP-ribosyl cyclase activation by angiotensin II in adult rat cardiomyocytes. Am J Physiol Heart Circ Physiol 295(1):H77-88. [PubMed: 18456728]  [MGI Ref ID J:138213]

Iqbal J; Zaidi M. 2006. TNF regulates cellular NAD+ metabolism in primary macrophages. Biochem Biophys Res Commun 342(4):1312-8. [PubMed: 16516847]  [MGI Ref ID J:107073]

Johnson JD; Ford EL; Bernal-Mizrachi E; Kusser KL; Luciani DS; Han Z; Tran H; Randall TD; Lund FE; Polonsky KS. 2006. Suppressed insulin signaling and increased apoptosis in CD38-null islets. Diabetes 55(10):2737-46. [PubMed: 17003338]  [MGI Ref ID J:116576]

Krebs C; Adriouch S; Braasch F; Koestner W; Leiter EH; Seman M; Lund FE; Oppenheimer N; Haag F; Koch-Nolte F. 2005. CD38 controls ADP-ribosyltransferase-2-catalyzed ADP-ribosylation of T cell surface proteins. J Immunol 174(6):3298-305. [PubMed: 15749861]  [MGI Ref ID J:97699]

Manjarrez-Orduno N; Moreno-Garcia ME; Fink K; Santos-Argumedo L. 2007. CD38 cross-linking enhances TLR-induced B cell proliferation but decreases IgM plasma cell differentiation. Eur J Immunol 37(2):358-67. [PubMed: 17274001]  [MGI Ref ID J:117900]

Mayo L; Jacob-Hirsch J; Amariglio N; Rechavi G; Moutin MJ; Lund FE; Stein R. 2008. Dual role of CD38 in microglial activation and activation-induced cell death. J Immunol 181(1):92-103. [PubMed: 18566373]  [MGI Ref ID J:137179]

Partida-Sanchez S; Cockayne DA; Monard S; Jacobson EL; Oppenheimer N; Garvy B; Kusser K; Goodrich S; Howard M; Harmsen A; Randall TD; Lund FE. 2001. Cyclic ADP-ribose production by CD38 regulates intracellular calcium release, extracellular calcium influx and chemotaxis in neutrophils and is required for bacterial clearance in vivo. Nat Med 7(11):1209-16. [PubMed: 11689885]  [MGI Ref ID J:126192]

Partida-Sanchez S; Goodrich S; Kusser K; Oppenheimer N; Randall TD; Lund FE. 2004. Regulation of dendritic cell trafficking by the ADP-ribosyl cyclase CD38: impact on the development of humoral immunity. Immunity 20(3):279-91. [PubMed: 15030772]  [MGI Ref ID J:89773]

Rodriguez-Alba JC; Moreno-Garcia ME; Sandoval-Montes C; Rosales-Garcia VH; Santos-Argumedo L. 2008. CD38 induces differentiation of immature transitional 2 B lymphocytes in the spleen. Blood 111(7):3644-52. [PubMed: 18223169]  [MGI Ref ID J:133536]

Shi G; Partida-Sanchez S; Misra RS; Tighe M; Borchers MT; Lee JJ; Simon MI; Lund FE. 2007. Identification of an alternative G{alpha}q-dependent chemokine receptor signal transduction pathway in dendritic cells and granulocytes. J Exp Med 204(11):2705-18. [PubMed: 17938235]  [MGI Ref ID J:126146]

Sun L; Iqbal J; Dolgilevich S; Yuen T; Wu XB; Moonga BS; Adebanjo OA; Bevis PJ; Lund F; Huang CL; Blair HC; Abe E; Zaidi M. 2003. Disordered osteoclast formation and function in a CD38 (ADP-ribosyl cyclase)-deficient mouse establishes an essential role for CD38 in bone resorption. FASEB J 17(3):369-75. [PubMed: 12631576]  [MGI Ref ID J:82191]

Young GS; Choleris E; Lund FE; Kirkland JB. 2006. Decreased cADPR and increased NAD+ in the Cd38-/- mouse. Biochem Biophys Res Commun 346(1):188-92. [PubMed: 16750163]  [MGI Ref ID J:110441]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.