Description

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System heterozygote x homozygote or homozygote x heterozygote Species laboratory mouse Generation F48+40+F8 (07-DEC-07)

Appearance
albino, ataxic
Related Genotype: a/a Tyr^c-2J/Tyr^c-2J awag/awag

albino, unaffected
Related Genotype: a/a Tyr^c-2J/Tyr^c-2J awag/+

Description
awag homozygotes have a distinct tremor when walking. When lifted by the tail these mutant mice do not splay their hind legs out as normal mice do, but instead hold their legs in a bowlegged umbrella manner and arch their backs. Both sexes breed and live a normal life span.

Development
The awag mutation arose spontaneously in 1998 on the C57BL/6J-Tyr^c-2J/J background (Stock No. 000058)in the production facility of The Jackson Laboratory where it was discovered by Jay Wellington. This mutant subline has been maintained by sibling mating ever since and this strain is still homozygous for Tyr^c-2J although maintained segregating for awag.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

awag/awag

        C57BL/6J Tyr^c-2J-awag/J

• behavior/neurological phenotype
• limb grasping (MGI Ref ID J:94744)
  • when lifted by the tail, mice do not splay hind legs out, but instead they hold their legs in a bowlegged umbrella like way and arch their backs
• tremors (MGI Ref ID J:94744)
  • distinct tremor when walking that first appears around 2 months of age
• hearing/vestibular/ear phenotype
• decreased brainstem auditory evoked potential (MGI Ref ID J:94744)
  • variable penetrance; ABR assesment at 3 months indicated that two mutants had hearing loss; other mice at 6 months had normal or nearly normal hearing
• vision/eye phenotype
• abnormal cornea morphology (MGI Ref ID J:94744)
  • corneal holes were observed, but is probably due to background strain and not the awag mutation
• cataracts (MGI Ref ID J:94744)
  • cataracts were observed, but is probably due to background strain and not the awag mutation
• nervous system phenotype
• hydroencephaly (MGI Ref ID J:94744)
  • mild hydrocephalus observed in three animals, assumed to be a strain background occurence

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

awag related

Neurobiology Research
Ataxia (Movement) Defects
Tremor Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		awag
Allele Name		ages with abnormal gait
Allele Type		Spontaneous
Strain of Origin		B6(Cg)-Tyrc-2J/J
Molecular Note		This spontaneous mutation was discovered in 1998 at The Jackson Laboratory.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Harris B; Ward-Bailey P; Johnson K; Bronson R; Davisson M. 2004. A new mouse mutation with an abnormal gait as it ages is mapped to mouse Chromosome 2 MGI Direct Data Submission :.  [MGI Ref ID J:94744]

Original article from The Mouse Mutant Resource

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Mating System	heterozygote x homozygote or homozygote x heterozygote

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

This strain cannot be phenotyped before eight weeks of age. Mice will be shipped at eight weeks of age or older. This strain is included in the Mouse Mutant Resource collection.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.