Strain Name: |
B6.Cg-Tg(Camk2a-cre)T29-1Stl/J |
|---|---|
Stock Number: |
005359 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Camk2a; cre; Tg(Camk2a-cre)T29-1Stl; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Generation N10F?+N1F5 (28-APR-08) Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer.Strain Development
A transgenic construct containing a 8.5 Kb sequence encoding the mouse calcium/calmodulin-dependent protein kinase II alpha promoter, sequence encoding Cre recombinase, and SV40 polyadenylation site sequence was injected into fertilized BCF1 eggs. The resulting male transgenic mice were bred to C57BL/6 mice, and then backcrossed to C57BL/6 mice for 10 generations.
Gene & Allele Details
| Allele Symbol | Tg(Camk2a-cre)T29-1Stl | ||
|---|---|---|---|
| Allele Name | transgene insertion T29-1, Susumu Tonegawa | ||
| Common Name(s) | CaMKIIalpha-cre; Cre; | ||
| Mutation Made By | Susumu Tonegawa, Massachusetts Institute of Technology | ||
| Strain of Origin | (C57BL x BALB/c)F1 | ||
| Site of Expression | forebrain; pyramidal cell layer | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| Molecular Note | This transgene directs the expression of Cre recombinase under the control of the alpha subunit of the calcium/calmodulin-dependent protein kinase II promoter. Cre recombinase activity was detected in the CA1 region of the hippocampus. [J:66344] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Camk2a
002362 B6.129P2-Camk2atm1Sva/J 007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005855 B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005087 C57BL/6-Tg(Camk2a-IDE)1Selk/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J View Strains carrying other alleles of Camk2a (10 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Internal/Organ Defects (brain)
Neurobiology Research
Channel and Transporter Defects
Cortical Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Cre-lox-System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox system)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Neurobiology Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesTsien JZ; Chen DF; Gerber D; Tom C; Mercer EH; Anderson DJ; Mayford M; Kandel ER; Tonegawa S. 1996. Subregion- and cell type-restricted gene knockout in mouse brain [see comments] Cell 87(7):1317-26. [PubMed: 8980237] [J:66344]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J |
| Stock Number: | 005359 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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