Strain Name: |
STOCK Tg(HIST1H2BB/EGFP)1Pa/J |
|---|---|
Stock Number: |
005418 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | ACTB; GFP; HIST1H2BB; Tg(HIST1H2BB/EGFP)1Pa; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Virginia Papaioannou, Columbia University Generation ?+N1F6 (30-APR-08) Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) under the control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. Nucleosomes and chromatin in all cells fluoresce. Fluorescence is detectable during all phases of mitosis. The donating investigator reports occasional silencing of the transgene when transmitted through the female germ line. This mutant mouse strain may be useful for in vivo subcellular high-resolution and 3-dimensional studies of dividing cells.Strain Development
A transgenic construct containing an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) was placed under the control of a chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer promoter. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. H2B-EGFP expressing ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to 129/Tac mice for germline transmission. Resulting founder animals were bred with outbred ICR mice prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(HIST1H2BB/EGFP)1Pa | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Virginia E Papaioannou | ||
| Common Name(s) | CAG::H2B-EGFP; H2B-EGFP; HBb-EGFP; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Nucleosomes and chromatin | ||
| Expressed Gene | HIST1H2BB, histone cluster 1, H2bb, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The coding sequence of EGFP was placed C-terminally to the coding sequence of the human histone H2B gene. When placed under control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer/promoter, a fluorescent fusion protein (H2B-EGFPis produced. [MGI Ref ID J:109836] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice are bred to wildtype siblings. Homozygotes, however, are fertile according to the donating investigator. The donating investigator also reports occasional silencing of the transgene when transmitted through the female germ line and recommends identifying mutant offspring by the fluorescent phenotype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
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006069 B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Transcriptional Regulation
Research Tools
Cell Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Hadjantonakis AK; Papaioannou VE. 2004. Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice. BMC Biotechnol 4:33. [PubMed: 15619330] [MGI Ref ID J:109836]
Price and Supply Information
| Strain Name: | STOCK Tg(HIST1H2BB/EGFP)1Pa/J |
| Stock Number: | 005418 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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