Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Jeffrey Chiang,   NCI/NIH

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of activated spleen lymphocytes. No telomerase activity is detected by telomeric repeat amplification (TRAP) assay analysis of testis cells. Mice homozygous for the targeted mutation lack telomerase activity and exhibit telomere shortening. Homozygous intercrossing for multiple generations causes telomere instability (shortening) and infertility. This mutant mouse strain may be useful in studies of telomerase function.

Development
A targeting vector containing Green Fluorescent Protein and neomycin resistance genes was used to disrupt exon 1. The construct was electroporated into 129S1/Sv-p⁺ Tyr⁺ Kitl^Sl-J derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 18 generations.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tert^tm1Yjc/Tert⁺

        involves: C57BL/6 * SPRET/Ei

• cellular phenotype
• *normal* cellular phenotype (MGI Ref ID J:92243)
  • telomere length is unaffected

Tert^tm1Yjc/Tert^tm1Yjc

        involves: C57BL/6 * SPRET/Ei

• cellular phenotype
• abnormal telomere length (MGI Ref ID J:92243)
  • telomeres are not elongated in Tert-deficient x SPRET/Ei mice as they are in Tert-sufficient x SPRET/Ei mice

Tert^tm1Yjc/Tert^tm1Yjc

        involves: 129/Sv * C57BL/6 * SJL * SPRET/Ei

• cellular phenotype
• abnormal telomere length (MGI Ref ID J:92243)
  • telomeres are not elongated in Terc-deficient x SPRET/Ei mice as they are in Terc-sufficient x SPRET/Ei mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
DNA Damage Response

Reproductive Biology Research
Fertility Defects

Research Tools
Cell Biology Research
Genetics Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Terttm1Yjc
Allele Name		targeted mutation 1, Y Jeffrey Chiang
Allele Type		Targeted (knock-out)
Common Name(s)		TERT-; mTERT-;
Mutation Made By		Jeffrey Chiang,   NCI/NIH
Gene Symbol and Name		Tert, telomerase reverse transcriptase
Chromosome		13
Gene Common Name(s)		EST2; TCS1; TP2; TR; TRT; hEST2;
Molecular Note		A GFPneo was inserted into the 5' UTR of exon 1. Southern blot and PCR confirmed recombination in mutant mice. RT-PCR showed a lack of transcript in mutants and a telomerase repeat amplification (TRAP) assay indicated there was no telomerase activity in testis cell extracts from mutants. [MGI Ref ID J:92243]

Genotyping

Genotyping Information

Genotyping Protocols

Tert^tm1Yjc, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chiang YJ; Hemann MT; Hathcock KS; Tessarollo L; Feigenbaum L; Hahn WC; Hodes RJ. 2004. Expression of telomerase RNA template, but not telomerase reverse transcriptase, is limiting for telomere length maintenance in vivo. Mol Cell Biol 24(16):7024-31. [PubMed: 15282303]  [MGI Ref ID J:92243]

Additional References

Tert^tm1Yjc related

Cheng A; Shin-ya K; Wan R; Tang SC; Miura T; Tang H; Khatri R; Gleichman M; Ouyang X; Liu D; Park HR; Chiang JY; Mattson MP. 2007. Telomere protection mechanisms change during neurogenesis and neuronal maturation: newly generated neurons are hypersensitive to telomere and DNA damage. J Neurosci 27(14):3722-33. [PubMed: 17409236]  [MGI Ref ID J:121221]

Chiang YJ; Kim SH; Tessarollo L; Campisi J; Hodes RJ. 2004. Telomere-associated protein TIN2 is essential for early embryonic development through a telomerase-independent pathway. Mol Cell Biol 24(15):6631-4. [PubMed: 15254230]  [MGI Ref ID J:92239]

Horikawa I; Chiang YJ; Patterson T; Feigenbaum L; Leem SH; Michishita E; Larionov V; Hodes RJ; Barrett JC. 2005. Differential cis-regulation of human versus mouse TERT gene expression in vivo: identification of a human-specific repressive element. Proc Natl Acad Sci U S A 102(51):18437-42. [PubMed: 16344462]  [MGI Ref ID J:117922]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes. Homozygous intercrossing for multiple generations causes infertility due to telomere shortening.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.