Strain Name:

B6.129S-Gpamtm1Rcol/J

Stock Number:

005429

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?+N1p (20-NOV-05)
Generation Definitions
 
Donating Investigator Rosalind Coleman,   University of North Carolina

Description
Mice that are homozygous for the targeted mutation are viable, fertile, and do not display any gross behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis. Enzyme activity levels in liver tissue are negligible. Residual activity is due to the inactivated microsomal isoform. Homozygotes exhibit reduced body weight. Female homozygotes weigh 20% less than wildtype controls at age 10 months. Male homozygotes do not exhibit as significant a weight reduction. Gonadal fat pad mass is reduced. Liver triacylglycerol and plasma lipid levels are reduced by 37% and 15% respectively. Very low density lipoprotein (VLDL) triacylglycerol level and secretion are decreased. Hepatic triacylglycerol fatty acid and phospholipid fatty acid compositions are abnormal with diminished palmitate content. F2 mice on a 50% C57BL/6J and 50% 129SvEv genetic background were used in all of the experiments described in the primary reference. This mutant mouse strain may be useful in studies of fatty acid metabolism, lipid homeostasis and triacylglycerol and phospholipid synthesis regulation.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 0.5kb of sequence encoding 62 amino acids in homology regions II and III. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to C57BL/6J female mice, and then backcrossed to the same for 6 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Gpamtm1Rcol/Gpamtm1Rcol

        involves: 129S6/SvEvTac * C57BL/6J
  • growth/size/body phenotype
  • decreased body weight
    • female homozygotes weigh 6% and 20% less than wild-type controls at 5 and 10 months, respectively   (MGI Ref ID J:84541)
    • although weights of male homozygotes do not differ significantly by genotype, pooled weight data indicates a significant effect of genotype, with mutant mice weighing less than wild-type controls   (MGI Ref ID J:84541)
    • slow postnatal weight gain
      • when fed a diet containing 14% kcal from fat, homozygotes gain less weight than control mice, as determined by body weights at 2, 5, 8, and 10 months of age   (MGI Ref ID J:84541)
  • decreased percent body fat
    • at 12 months of age, homozygotes display less fat than wild-type controls as a percentage of total body weight   (MGI Ref ID J:84541)
  • adipose tissue phenotype
  • decreased gonadal fat pad weight
    • at 12 months of age, homozygotes display reduced gonadal fat pad weight relative to wild-type controls   (MGI Ref ID J:84541)
  • decreased percent body fat
    • at 12 months of age, homozygotes display less fat than wild-type controls as a percentage of total body weight   (MGI Ref ID J:84541)
  • homeostasis/metabolism phenotype
  • abnormal lipid homeostasis
    • in mutant liver, the palmitate content is lower in triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine   (MGI Ref ID J:84541)
    • in mutant liver, phosphatidylethanolamine and phosphatidylcholine contains about 21% less palmitate in the sn-1 position and 36 and 40%, respectively, more arachidonate in the sn-2 position   (MGI Ref ID J:84541)
    • abnormal fatty acid level
      • homozygotes display changes in fatty acid content of triacylglycerol and cholesterol esters, and altered positioning of specific fatty acids, particularly palmitate and arachidonate, in phospholipids   (MGI Ref ID J:84541)
    • decreased circulating cholesterol level
      • at 2 months of age, homozygotes display significantly lower total plasma cholesterol levels relative to control mice   (MGI Ref ID J:84541)
      • decreased circulating HDL cholesterol level
        • at 2 months of age, female homozygotes display reduced plasma HDL cholesterol levels relative to control mice   (MGI Ref ID J:84541)
    • decreased circulating triglyceride level
      • at 2 months of age, female homozygotes show a 15% reduction in plasma triacylglycerol levels relative to control mice   (MGI Ref ID J:84541)
      • reduced plasma triacylglycerol levels are due to a 30% decrease in VLDL secretion rate   (MGI Ref ID J:84541)
      • decreased circulating VLDL triglyceride level
        • at 2 months of age, female homozygotes display reduced VLDL triacylglycerol levels relative to control mice   (MGI Ref ID J:84541)
    • decreased liver triglyceride level
      • at 2 months of age, female homozygotes show a 37% reduction in liver triacylglycerol content relative to control mice   (MGI Ref ID J:84541)
      • in contrast, hepatic cholesterol and phospholipid contents remain normal   (MGI Ref ID J:84541)
  • decreased circulating insulin level
    • at 2 months of age, homozygotes display a trend toward reduced plasma insulin levels, suggesting increased insulin sensitivity   (MGI Ref ID J:84541)
  • liver/biliary system phenotype
  • decreased liver triglyceride level
    • at 2 months of age, female homozygotes show a 37% reduction in liver triacylglycerol content relative to control mice   (MGI Ref ID J:84541)
    • in contrast, hepatic cholesterol and phospholipid contents remain normal   (MGI Ref ID J:84541)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Other
      altered lipoprotein profile

Internal/Organ Research
Adipose Defects

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gpamtm1Rcol
Allele Name targeted mutation 1, Rosalind Coleman
Allele Type Targeted (Null/Knockout)
Common Name(s) Gpat1-; mtGPAT-;
Mutation Made By Rosalind Coleman,   University of North Carolina
Strain of Origin129S6/SvEvTac
ES Cell Line NameTC1/TC-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Gpam, glycerol-3-phosphate acyltransferase, mitochondrial
Chromosome 19
Gene Common Name(s) GPAT; GPAT1;
Molecular Note A 0.5 kb genomic fragment encoding 62 amino acids including those that make up homology regions II and III was replaced with a neo cassette inserted by homologous recombination. Transcript was undetected by Northern blot analysis of homozygous mutant liver tissue. Activity assays indicated a near comlpete ablation of endogenous activity and showed that the activity of the microsomal isoform was unperturbed. [MGI Ref ID J:84541]

Genotyping

Genotyping Information

Genotyping Protocols

Gpamtm1Rcol, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hammond LE; Gallagher PA; Wang S; Hiller S; Kluckman KD; Posey-Marcos EL; Maeda N; Coleman RA. 2002. Mitochondrial glycerol-3-phosphate acyltransferase-deficient mice have reduced weight and liver triacylglycerol content and altered glycerolipid fatty acid composition. Mol Cell Biol 22(23):8204-14. [PubMed: 12417724]  [MGI Ref ID J:84541]

Additional References

Gpamtm1Rcol related

Collison LW; Murphy EJ; Jolly CA. 2008. Glycerol-3-phosphate acyltransferase-1 regulates murine T-lymphocyte proliferation and cytokine production. Am J Physiol Cell Physiol 295(6):C1543-9. [PubMed: 18971390]  [MGI Ref ID J:143259]

Gonzalez-Baro MR; Lewin TM; Coleman RA. 2007. Regulation of Triglyceride Metabolism. II. Function of mitochondrial GPAT1 in the regulation of triacylglycerol biosynthesis and insulin action. Am J Physiol Gastrointest Liver Physiol 292(5):G1195-9. [PubMed: 17158253]  [MGI Ref ID J:122621]

Hammond LE; Neschen S; Romanelli AJ; Cline GW; Ilkayeva OR; Shulman GI; Muoio DM; Coleman RA. 2005. Mitochondrial glycerol-3-phosphate acyltransferase-1 is essential in liver for the metabolism of excess acyl-CoAs. J Biol Chem 280(27):25629-36. [PubMed: 15878874]  [MGI Ref ID J:106957]

Karlsson EA; Wang S; Shi Q; Coleman RA; Beck MA. 2009. Glycerol-3-phosphate acyltransferase 1 is essential for the immune response to infection with coxsackievirus B3 in mice. J Nutr 139(4):779-83. [PubMed: 19193813]  [MGI Ref ID J:146502]

Lewin TM; Schwerbrock NM; Lee DP; Coleman RA. 2004. Identification of a new glycerol-3-phosphate acyltransferase isoenzyme, mtGPAT2, in mitochondria. J Biol Chem 279(14):13488-95. [PubMed: 14724270]  [MGI Ref ID J:89170]

Lewin TM; de Jong H; Schwerbrock NJ; Hammond LE; Watkins SM; Combs TP; Coleman RA. 2008. Mice deficient in mitochondrial glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition. Biochim Biophys Acta 1781(6-7):352-8. [PubMed: 18522808]  [MGI Ref ID J:137056]

Neschen S; Morino K; Hammond LE; Zhang D; Liu ZX; Romanelli AJ; Cline GW; Pongratz RL; Zhang XM; Choi CS; Coleman RA; Shulman GI. 2005. Prevention of hepatic steatosis and hepatic insulin resistance in mitochondrial acyl-CoA:glycerol-sn-3-phosphate acyltransferase 1 knockout mice. Cell Metab 2(1):55-65. [PubMed: 16054099]  [MGI Ref ID J:129839]

Park YK; Park B; Lee S; Choi K; Moon Y; Park H. 2013. Hypoxia-inducible factor-2alpha-dependent hypoxic induction of Wnt10b expression in adipogenic cells. J Biol Chem 288(36):26311-22. [PubMed: 23900840]  [MGI Ref ID J:203532]

Wendel AA; Li LO; Li Y; Cline GW; Shulman GI; Coleman RA. 2010. Glycerol-3-phosphate acyltransferase 1 deficiency in ob/ob mice diminishes hepatic steatosis but does not protect against insulin resistance or obesity. Diabetes 59(6):1321-9. [PubMed: 20200319]  [MGI Ref ID J:169655]

Zhang C; Wendel AA; Keogh MR; Harris TE; Chen J; Coleman RA. 2012. Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. Proc Natl Acad Sci U S A 109(5):1667-72. [PubMed: 22307628]  [MGI Ref ID J:182033]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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