Strain Name: |
C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ |
|---|---|
Stock Number: |
005431 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(Ins2-TFRC/OV)296Wehi/WehiJ (Changed: 01-FEB-07
) |
| Genes & Alleles | Ins2; OVA; TFRC; Tg(Ins2-TFRC/OVA)296Wehi; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator William Heath, The Walter and Eliza Hall Institute H2 Haplotype b Generation N20+N1F4 (05-JAN-08) Appearance
black
Related Genotype: a/aStrain Description
Ins2-TFRC/OVA (commonly referred to as RIP-mOVA) line 296-1B hemizygote transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis detects strong expression in pancreatic beta cells and kidney proximal tubular cells and weak expression in testes.When C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ is mated to C57BL/6-Tg(TcraTcrb)1100Mjb/J (commonly referred to as OT-1 and recognizes OVA specific T-cells, Stock No. 003831) thymic deletion of OT-1 cells is observed in double transgenic mice, suggesting very low levels of thymic expression. OVA specific CD-8+ T-cells activated by cross presentation infiltrate the pancreas causing beta cell destruction resulting in diabetes. However, these cells do not infiltrate the kidney.
Proliferating OT-1 T-cells appeared specifically in the lymph nodes draining the pancreas (PLN's) and kidney (RLN's) on day 3 after adoptive transfer of naive OT-1 cells into adult C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ hosts. In contrast, no proliferating cells were detected in the PLN's after introduction into 10-d-old recipients, although they were observed in significant numbers in the RLNs of these animals.
This transgenic model has been used to study ectopic expression in the thymus and for assessing the requirements for peripheral deletion.
Strain Development
The fusion transgene TFRC/OVA is under the control of the rat insulin promoter (Ins2) and encodes the first 118 residues of the human transferrin receptor (TFRC), which includes the cytoplasmic tail and signal/anchor domain, fused to mature chicken ovalbumin (OVA), residues 139-38, resulting in a membrane bound form of ovalbumin. This transgenic construct was injected into C57BL/6J oocytes and founder, 296-1B, was backcrossed to C57BL/6J for 20 generations (C Kurts, H Kosaka et al, 1996). In 2005, transgenic line 296-1B arrived at The Jackson Laboratory, was backcrossed to C57BL/6J one generation and is maintained Tg/? x Tg/?.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-TFRC/OVA)296Wehi | ||
|---|---|---|---|
| Allele Name | transgene insertion 296, Walter and Eliza Hall Institute of Medical Research | ||
| Common Name(s) | RIP-mOVA; | ||
| Mutation Made By | William Heath, The Walter and Eliza Hall Institute | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | TFRC, transferrin receptor (p90, CD71), human | ||
| Expressed Gene | OVA, ovalbumin, chicken | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Ovalbumin (OV) is present at high levels in pancreatic beta cells and in kidney proximal tubule cells and at very low levels in testes of transgenic mice. No OV has been detected immunohistologically in any of multiple other tissues examined. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an OV-specific T cell receptor expressed on CD8+ T lymphocytes, exhibit thymic deletion of OT-1 T cells, indicating presence of OV at very low levels in the thymus. Western blot analysis reproducibly measured the pancreatic islet OV content as 2.2 ng/ug of protein. | ||
| Molecular Note | The transgene encodes a fusion protein comprising the carboxy-terminal 118 amino acids of the human transferrin receptor, which include the cytoplasmic tail, membrane-targeting signal and anchor domain of the protein, joined to amino acids 139-385 of mature ovalbumin. Its expression is directed by the rat insulin 2 promoter. [MGI Ref ID J:98589] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005432 C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ 005433 C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
Animal Health Reports
Room Number FGB29
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)
References
Selected Reference(s)
Additional ReferencesKurts C; Heath WR; Carbone FR; Allison J; Miller JF; Kosaka H. 1996. Constitutive class I-restricted exogenous presentation of self antigens in vivo. J Exp Med 184(3):923-30. [PubMed: 9064352] [MGI Ref ID J:98589]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ |
| Stock Number: | 005431 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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