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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ (Changed: 01-FEB-07 ) Type Mutant Strain; Transgenic; Mating System Tg/? x Tg/? (Female x Male) Species laboratory mouse H2 Haplotype b Generation N7+N1F9 (12-MAY-08) Donating Investigator William Heath, The Walter and Eliza Hall Institute Appearance
black
Related Genotype: a/aDescription
Immunohistochemistry does not detect ovalbumin in the islet beta cells of the pancreas. The double transgenic resulting from a cross of this transgenic stock with C57BL/6-Tg(Tcra Tcrb)1100MjbJ (OT-1) exhibit early onset of spontaneous diabetes with islet infiltration; implying that the beta cells express OVA. One of 60 irradiated Tg(Ins2-OV)307 mice receiving a 1:4 mixture (OT-1 transgenic mice:C57BL/6-Thy1.1 congenic) of bone marrow become diabetic when followed for 10 weeks post transfer. Histolocigal evaluation indicates 22% of the islets are mildly infiltrated 15-21 weeks post transfer. This stock only exhibits antigen presentation in the draining lymph node when the pancreatic islets have been damaged.This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.
Development
C57BL/6-Tg(Ins2-OV)59Wehi/WehiJ expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2bm1 oocytes. Resulting progeny from founder 307-3 were backcrossed to C57BL/6 for 5 generations prior to intercrossing. The Jackson Laboratory received C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ at generation N7.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005433 C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ 005431 C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Ins2-OVA)307Wehi/?
involves: BALB/c * C57BL/6
- immune system phenotype
- increased susceptibility to autoimmune diabetes (MGI Ref ID J:99560)
- mice die from diabetes within 12 days of transfer of OVA-specific CD8 T cells
- digestive/alimentary phenotype
- abnormal pancreatic beta cell physiology (MGI Ref ID J:99560)
- beta cells secrete very low amounts of a soluble form of chicken ovalbumin (OVA)
- secretion is inferred by the infiltration of ovalbumin specific T cells
- endocrine/exocrine gland phenotype
- abnormal pancreatic beta cell physiology (MGI Ref ID J:99560)
- beta cells secrete very low amounts of a soluble form of chicken ovalbumin (OVA)
- secretion is inferred by the infiltration of ovalbumin specific T cells
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins2-OVA)307Wehi | ||
|---|---|---|---|
| Allele Name | transgene insertion 307, Walter and Eliza Hall Institute of Medical Research | ||
| Common Name(s) | RIP-OVA; RIP-OVAlo; | ||
| Mutation Made By | William Heath, The Walter and Eliza Hall Institute | ||
| Strain of Origin | B6.C-H2 | ||
| Expressed Gene | OVA, ovalbumin, chicken | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets. | ||
| Molecular Note | This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. [MGI Ref ID J:99451] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ins2-OVA), STD PCR, vers. 1
Tg(Ins2-OVA), STD PCR, vers. 1
Tg(Ins2-OVA), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Blanas E; Carbone FR; Allison J; Miller JF; Heath WR. 1996. Induction of autoimmune diabetes by oral administration of autoantigen. Science 274(5293):1707-9. [PubMed: 8939860] [MGI Ref ID J:99451]
Additional References
Tg(Ins2-OVA)307Wehi related
Belz GT; Vremec D; Febbraio M; Corcoran L; Shortman K; Carbone FR; Heath WR. 2002. CD36 is differentially expressed by CD8+ splenic dendritic cells but is not required for cross-presentation in vivo. J Immunol 168(12):6066-70. [PubMed: 12055215] [MGI Ref ID J:123014]Hanninen A; Braakhuis A; Heath WR; Harrison LC. 2001. Mucosal antigen primes diabetogenic cytotoxic T-lymphocytes regardless of dose or delivery route. Diabetes 50(4):771-5. [PubMed: 11289041] [MGI Ref ID J:99557]
Hanninen A; Martinez NR; Davey GM; Heath WR; Harrison LC. 2002. Transient blockade of CD40 ligand dissociates pathogenic from protective mucosal immunity. J Clin Invest 109(2):261-7. [PubMed: 11805138] [MGI Ref ID J:74333]
Hanninen A; Nurmela R; Maksimow M; Heino J; Jalkanen S; Kurts C. 2007. Islet beta-cell-specific T cells can use different homing mechanisms to infiltrate and destroy pancreatic islets. Am J Pathol 170(1):240-50. [PubMed: 17200197] [MGI Ref ID J:117052]
Kurts C; Miller JF; Subramaniam RM; Carbone FR; Heath WR. 1998. Major histocompatibility complex class I-restricted cross-presentation is biased towards high dose antigens and those released during cellular destruction. J Exp Med 188(2):409-14. [PubMed: 9670054] [MGI Ref ID J:99560]
Kurts C; Sutherland RM; Davey G; Li M; Lew AM; Blanas E; Carbone FR; Miller JF; Heath WR. 1999. CD8 T cell ignorance or tolerance to islet antigens depends on antigen dose. Proc Natl Acad Sci U S A 96(22):12703-7. [PubMed: 10535986] [MGI Ref ID J:99559]
Nopora A; Brocker T. 2002. Bcl-2 controls dendritic cell longevity in vivo. J Immunol 169(6):3006-14. [PubMed: 12218115] [MGI Ref ID J:132008]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $54.00 Female or Male Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/? *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $108.00 Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/? x Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/?
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $70.20 Female or Male Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/? *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $140.40 Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/? x Hemizygous or Homozygous for Tg(Ins2-OVA)307Wehi/WehiJ - Tg/?
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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