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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ (Changed: 01-FEB-07 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse H2 Haplotype b Generation N7+N1F0+N1p (20-NOV-05)
Generation DefinitionsDonating Investigator William Heath, The Walter and Eliza Hall Institute Appearance
black
Related Genotype: a/aDescription
Immunohistochemistry does not detect ovalbumin in the islet beta cells of the pancreas. The double transgenic resulting from a cross of this transgenic stock with C57BL/6-Tg(Tcra Tcrb)1100MjbJ (OT-1) exhibit early onset of spontaneous diabetes with islet infiltration; implying that the beta cells express OVA. One of 60 irradiated Tg(Ins2-OV)307 mice receiving a 1:4 mixture (OT-1 transgenic mice:C57BL/6-Thy1.1 congenic) of bone marrow become diabetic when followed for 10 weeks post transfer. Histolocigal evaluation indicates 22% of the islets are mildly infiltrated 15-21 weeks post transfer. This stock only exhibits antigen presentation in the draining lymph node when the pancreatic islets have been damaged.This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.
Development
C57BL/6-Tg(Ins2-OV)59Wehi/WehiJ expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2bm1 oocytes. Resulting progeny from founder 307-3 were backcrossed to C57BL/6 for 5 generations prior to intercrossing. The Jackson Laboratory received C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ at generation N7.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (46 strains)
005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005433 C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ 005431 C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Ins2-OVA)307Wehi/?
involves: BALB/c * C57BL/6
- immune system phenotype
- increased susceptibility to autoimmune diabetes
- mice die from diabetes within 12 days of transfer of OVA-specific CD8 T cells (MGI Ref ID J:99560)
- endocrine/exocrine gland phenotype
- abnormal pancreatic beta cell physiology
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Type 1 Diabetes
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research
T Cell Receptor Transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins2-OVA)307Wehi | ||
|---|---|---|---|
| Allele Name | transgene insertion 307, Walter and Eliza Hall Institute of Medical Research | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | RIP-OVA; RIP-OVAlo; | ||
| Mutation Made By | William Heath, The Walter and Eliza Hall Institute | ||
| Strain of Origin | B6.C-H2 | ||
| Expressed Gene | OVA, ovalbumin, chicken | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets. | ||
| Molecular Note | This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. [MGI Ref ID J:99451] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ins2-OVA), QPCR
Tg(Ins2-OVA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Blanas E; Carbone FR; Allison J; Miller JF; Heath WR. 1996. Induction of autoimmune diabetes by oral administration of autoantigen. Science 274(5293):1707-9. [PubMed: 8939860] [MGI Ref ID J:99451]
Additional References
Tg(Ins2-OVA)307Wehi relatedBelz GT; Vremec D; Febbraio M; Corcoran L; Shortman K; Carbone FR; Heath WR. 2002. CD36 is differentially expressed by CD8+ splenic dendritic cells but is not required for cross-presentation in vivo. J Immunol 168(12):6066-70. [PubMed: 12055215] [MGI Ref ID J:123014]
Flatz L; Hegazy AN; Bergthaler A; Verschoor A; Claus C; Fernandez M; Gattinoni L; Johnson S; Kreppel F; Kochanek S; Broek M; Radbruch A; Levy F; Lambert PH; Siegrist CA; Restifo NP; Lohning M; Ochsenbein AF; Nabel GJ; Pinschewer DD. 2010. Development of replication-defective lymphocytic choriomeningitis virus vectors for the induction of potent CD8+ T cell immunity. Nat Med 16(3):339-45. [PubMed: 20139992] [MGI Ref ID J:158626]
Hanninen A; Braakhuis A; Heath WR; Harrison LC. 2001. Mucosal antigen primes diabetogenic cytotoxic T-lymphocytes regardless of dose or delivery route. Diabetes 50(4):771-5. [PubMed: 11289041] [MGI Ref ID J:99557]
Hanninen A; Martinez NR; Davey GM; Heath WR; Harrison LC. 2002. Transient blockade of CD40 ligand dissociates pathogenic from protective mucosal immunity. J Clin Invest 109(2):261-7. [PubMed: 11805138] [MGI Ref ID J:74333]
Hanninen A; Nurmela R; Maksimow M; Heino J; Jalkanen S; Kurts C. 2007. Islet beta-cell-specific T cells can use different homing mechanisms to infiltrate and destroy pancreatic islets. Am J Pathol 170(1):240-50. [PubMed: 17200197] [MGI Ref ID J:117052]
Kenna TJ; Waldie T; McNally A; Thomson M; Yagita H; Thomas R; Steptoe RJ. 2010. Targeting antigen to diverse APCs inactivates memory CD8+ T cells without eliciting tissue-destructive effector function. J Immunol 184(2):598-606. [PubMed: 19995901] [MGI Ref ID J:159425]
Klages K; Mayer CT; Lahl K; Loddenkemper C; Teng MW; Ngiow SF; Smyth MJ; Hamann A; Huehn J; Sparwasser T. 2010. Selective depletion of Foxp3+ regulatory T cells improves effective therapeutic vaccination against established melanoma. Cancer Res 70(20):7788-99. [PubMed: 20924102] [MGI Ref ID J:165566]
Kurts C; Miller JF; Subramaniam RM; Carbone FR; Heath WR. 1998. Major histocompatibility complex class I-restricted cross-presentation is biased towards high dose antigens and those released during cellular destruction. J Exp Med 188(2):409-14. [PubMed: 9670054] [MGI Ref ID J:99560]
Kurts C; Sutherland RM; Davey G; Li M; Lew AM; Blanas E; Carbone FR; Miller JF; Heath WR. 1999. CD8 T cell ignorance or tolerance to islet antigens depends on antigen dose. Proc Natl Acad Sci U S A 96(22):12703-7. [PubMed: 10535986] [MGI Ref ID J:99559]
Nopora A; Brocker T. 2002. Bcl-2 controls dendritic cell longevity in vivo. J Immunol 169(6):3006-14. [PubMed: 12218115] [MGI Ref ID J:132008]
Sabarth N; Chamberlain L; Brett S; Tite J; Craigen J. 2010. Induction of homologous rather than heterologous antigen-specific CD4 T cell responses is critical for functional CD8 T cell responses in mice transgenic for a foreign antigen. J Immunol 185(8):4590-601. [PubMed: 20861346] [MGI Ref ID J:164726]
Sutherland AP; Joller N; Michaud M; Liu SM; Kuchroo VK; Grusby MJ. 2013. IL-21 Promotes CD8+ CTL Activity via the Transcription Factor T-bet. J Immunol 190(8):3977-84. [PubMed: 23479229] [MGI Ref ID J:194899]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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