Strain Name: |
C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ |
|---|---|
Stock Number: |
005433 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(Ins2-OV)59Wehi/WehiJ (Changed: 01-FEB-07
) |
| Genes & Alleles | Ins2; OVA; Tg(Ins2-OVA)59Wehi; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator William Heath, The Walter and Eliza Hall Institute H2 Haplotype b Generation N4F15+F9 (10-DEC-07) Appearance
black
Related Genotype: a/aStrain Description
Transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The pancreatic islets exhibit weak ovalbumin- (OV-) specific immunohistologic staining and OV is detectable in the islets by Western blot analysis. These mice develop diabetes following adoptive transfer of activated, but not naive, CD8+ T lymphocytes from mice bearing Tg(TcraTcrb)1100Mjb (OT-1). Both CD8+ T-cells and CD4+ T cells from these transgenic animals are tolerant to ovalbumin.This model provides a tool for assessing the requirements of peripheral T-cell deletion.
Strain Development
C57Bl/6-Tg(Ins2-OV)59Wehi/WehiJ expresses expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2bm1 oocytes. Founder L59-2 was backcrossed to C57BL/6 for 4 generations prior to intercrossing. The Jackson Laboratory received C57Bl/6-Tg(Ins2-OV)59Wehi/WehiJ at generation N4F15.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-OVA)59Wehi | ||
|---|---|---|---|
| Allele Name | transgene insertion 59, Walter and Eliza Hall Institute of Medical Research | ||
| Common Name(s) | RIP-OVAhi; | ||
| Mutation Made By | William Heath, The Walter and Eliza Hall Institute | ||
| Strain of Origin | B6.C-H2bm1/ByJWehi | ||
| Expressed Gene | OVA, ovalbumin, chicken | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | The pancreatic islets of transgenic mice exhibit weak ovalbumin- (OV-) specific immunohistologic staining. Western blot analysis measures the islet OV content as 1.0 +/- 0.4 ng/'ug of protein. These mice develop diabetes following adoptive transfer of activated, but not naive, CD8+ T lymphocytes from mice bearing Tg(TcraTcrb)1100Mjb (OT-1). | ||
| Molecular Note | Mice bearing this transgene express a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. [MGI Ref ID J:99451] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-OVA)
Tg(Ins2-OVA)59Wehi QPCR
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005432 C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ 005431 C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)
References
Selected Reference(s)
Additional ReferencesKurts C; Miller JF; Subramaniam RM; Carbone FR; Heath WR. 1998. Major histocompatibility complex class I-restricted cross-presentation is biased towards high dose antigens and those released during cellular destruction. J Exp Med 188(2):409-14. [PubMed: 9670054] [MGI Ref ID J:99560]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ |
| Stock Number: | 005433 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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