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Strain Name: |
STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J |
Stock Number: |
005438 |
Availability:
| Repository-Cryopreserved |
| Former Name |
STOCK Tg(ACTB-Bgeo,-DsRed*MST)1Nagy/J (Changed: 08-MAY-08
)
|
| Genes & Alleles |
ACTB;
Bgeo;
DsRed;
Tg(CAG-Bgeo,-DsRed*MST)1Nagy;
|
Product Information
Strain Details
| Type |
JAX® GEMM® Strain -
Mutant Stock |
| Additional information on
JAX® GEMM® Strains. |
| Type |
JAX® GEMM® Strain -
Mutant Strain |
| Type |
JAX® GEMM® Strain -
Transgenic |
| Species | laboratory mouse |
| Donating Investigator | Andras Nagy, Mount Sinai Hospital |
|
|
Strain Description
While mice hemizygous for the transgenic insert are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past 5 weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
Strain Development
A transgenic construct containing a Red Fluorescent Protein variant (DsRed.MST) gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. ES cell clone containing one copy of the transgene and expressing LacZ was aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross, that were hemizygous for the transgene, were bred to ICR outbred mice. While at The Jackson Laboratory, hemizygous mice are bred together, and may also be bred to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability.
Gene & Allele Details
| Allele Symbol |
Tg(CAG-Bgeo,-DsRed*MST)1Nagy |
| Allele Name |
transgene insertion 1, Andras Nagy |
| Common Name(s) |
11/C1;
Tg(ACTB-Bgeo,-DsRed*MST)1Nagy;
Z/RED;
|
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI |
| Strain of Origin | (129S6/SvEvTac x C57BL/6)F1 |
| ES Cell Line Name | Other (see notes) |
| Site of Expression | lacZ is expressed in embryonic and adult tissues; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with Red Fluorescent Protein variant (DsRed.MST) expression |
| Expressed Gene |
DsRed, red fluorescent protein, |
| Expressed Gene |
Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes
, E. coli |
| Promoter |
ACTB, actin, beta, human |
| General Note |
The transgenic ES cell clone 11/C1, created by electroporation of (129S6/SvEvTac x C57BL/6)F1-derived G4 ES cells, was used to generate transgenic mice. The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218). |
| Molecular Note |
The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. [J:109837]
|
Control Information
Genotyping Protocols
ACTB-DsRed.MST
Bgeo
Colony Maintenance
| Breeding & Husbandry | It has been our experience at The Jackson Laboratory that hemizygotes maintained on a mixed genetic background are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Additionally, smaller mice or mice with improper tooth development may benefit from adding pulverized (and/or increased fat) chow to the cage floor prior to and after weaning to promote the survival of the transgenic pups. Although homozygous animals are born, animals have not survived past 5 weeks of age. Given this, as well as the possibility that fluorescent protein polymer formation may result in DsRed-expressing mice, hemizygous mice are bred to wildtype siblings or to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability. |
| Diet Information |
LabDiet® 5K52/5K67
|
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| 005076 | NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ |
| 005082 | NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ |
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View Fluorescent Protein Strains (138 strains)
lacZ Expression Strains
| 002484 | 129-Alpltm1Sor/J |
| 002292 | 129-Gt(ROSA)26Sor/J |
| 006050 | 129-Sirt6tm1Fwa/J |
| 003451 | 129-Smad3tm1Par/J |
| 003310 | 129S-Gt(ROSA)26Sortm1Sor/J |
| 003383 | 129S-Nogtm1Amc/J |
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| 005091 | 129S-Pnpla6tm1Blw/J |
| 007199 | 129S-Sgpl1Gt(ROSA)78Sor/J |
| 003082 | 129S1/SvImJ-Bcl2tm1Mpin/J |
| 004478 | B6.129-Foxd1tm1Lai/J |
| 006939 | B6.129-Fut1tm1Sdo/J |
| 005768 | B6.129-Htr5atm1Dgen/J |
| 002938 | B6.129-Kdrtm1Jrt/J |
| 004158 | B6.129-Maftm1Gsb/J |
| 006497 | B6.129-Skiltm2Spw/J |
| 005772 | B6.129P2-Acvrl1tm1Dgen/J |
| 006431 | B6.129P2-Adam21tm1Dgen/J |
| 005770 | B6.129P2-Adamts4tm1Dgen/J |
| 005771 | B6.129P2-Adamts5tm1Dgen/J |
| 005773 | B6.129P2-Adcy3tm1Dgen/J |
| 005774 | B6.129P2-Adcy7tm1Dgen/J |
| 005775 | B6.129P2-Adipor2tm1Dgen/J |
| 005776 | B6.129P2-Avpr1atm1Dgen/J |
| 005777 | B6.129P2-Axltm1Dgen/J |
| 005783 | B6.129P2-Cacna1ctm1Dgen/J |
| 005780 | B6.129P2-Cacna2d3tm1Dgen/J |
| 005781 | B6.129P2-Cacng3tm1Dgen/J |
| 005782 | B6.129P2-Cacng4tm1Dgen/J |
| 005784 | B6.129P2-Capn5tm1Dgen/J |
| 005785 | B6.129P2-Capn7tm1Dgen/J |
| 005792 | B6.129P2-Ccr1l1tm1Dgen/J |
| 005793 | B6.129P2-Ccr6tm1Dgen/J |
| 005794 | B6.129P2-Ccr7tm1Dgen/J |
| 005779 | B6.129P2-Celsr2tm1Dgen/J |
| 005797 | B6.129P2-Chrna2tm1Dgen/J |
| 005787 | B6.129P2-Ctsctm1Dgen/J |
| 005796 | B6.129P2-Cxcr3tm1Dgen/J |
| 005798 | B6.129P2-Drd5tm1Dgen/J |
| 005800 | B6.129P2-Efemp2tm1Dgen/J |
| 005801 | B6.129P2-Esrratm1Dgen/J |
| 005802 | B6.129P2-Faim2tm1Dgen/J |
| 006262 | B6.129P2-Fut2tm1Sdo/J |
| 005803 | B6.129P2-Fzd1tm1Dgen/J |
| 005804 | B6.129P2-Fzd8tm1Dgen/J |
| 005811 | B6.129P2-Gabra3tm1Dgen/J |
| 005812 | B6.129P2-Gabra4tm1Dgen/J |
| 005810 | B6.129P2-Gabrptm1Dgen/J |
| 005809 | B6.129P2-Galr1tm1Dgen/J |
| 005816 | B6.129P2-Glra3tm1Dgen/J |
| 005805 | B6.129P2-Gpr151tm1Dgen/J |
| 005806 | B6.129P2-Gpr37tm1Dgen/J |
| 005807 | B6.129P2-Gpr6tm1Dgen/J |
| 005813 | B6.129P2-Grik5tm1Dgen/J |
| 005808 | B6.129P2-Grk5tm1Dgen/J |
| 005814 | B6.129P2-Grm1tm1Dgen/J |
| 005815 | B6.129P2-Grm3tm1Dgen/J |
| 005817 | B6.129P2-Gsk3btm1Dgen/J |
| 005818 | B6.129P2-Hcrtr1tm1Dgen/J |
| 005767 | B6.129P2-Htr4tm1Dgen/J |
| 005769 | B6.129P2-Htr7tm1Dgen/J |
| 005830 | B6.129P2-Kcnq2tm1Dgen/J |
| 005821 | B6.129P2-Lats2tm1Dgen/J |
| 005822 | B6.129P2-Lmbr1tm1Dgen/J |
| 005850 | B6.129P2-Mapkapk2tm1Dgen/J |
| 005824 | B6.129P2-Mmp17tm1Dgen/J |
| 005825 | B6.129P2-Mtmr1tm1Dgen/J |
| 005778 | B6.129P2-Naip1tm1Dgen/J |
| 005826 | B6.129P2-Ntsr1tm1Dgen/J |
| 005829 | B6.129P2-Pkd2l2tm1Dgen/J |
| 005828 | B6.129P2-Ppardtm1Dgen/J |
| 005831 | B6.129P2-Ppm1ftm1Dgen/J |
| 005827 | B6.129P2-Ptch2tm1Dgen/J |
| 005832 | B6.129P2-Ptprotm1Dgen/J |
| 005799 | B6.129P2-S1pr4tm1Dgen/J |
| 005837 | B6.129P2-Scn11atm1Dgen/J |
| 005836 | B6.129P2-Scn9atm1Dgen/J |
| 005834 | B6.129P2-Sema5atm1Dgen/J |
| 005835 | B6.129P2-Sema6ctm1Dgen/J |
| 006432 | B6.129P2-Slc18a1tm1Dgen/J |
| 005839 | B6.129P2-Slc22a12tm1Dgen/J |
| 005838 | B6.129P2-Slc22a6tm1Dgen/J |
| 005840 | B6.129P2-Slc40a1tm1Dgen/J |
| 005841 | B6.129P2-Slc6a9tm1Dgen/J |
| 005842 | B6.129P2-Slc7a8tm1Dgen/J |
| 005843 | B6.129P2-Slc9a6tm1Dgen/J |
| 005844 | B6.129P2-Sstr1tm1Dgen/J |
| 005847 | B6.129P2-Tgfbr1tm1Dgen/J |
| 005845 | B6.129P2-Thbs4tm1Dgen/J |
| 005790 | B6.129P2-Tpp1tm1Dgen/J |
| 005848 | B6.129P2-Trpm5tm1Dgen/J |
| 005791 | B6.129P2-Xcr1tm1Dgen/J |
| 003474 | B6.129S4-Gt(ROSA)26Sortm1Sor/J |
| 005901 | B6.129S4-Ppardtm2Rev/J |
| 006142 | B6.129S4-Ppargtm1Rev/J |
| 003754 | B6.129S4-Shroom3Gt(ROSA)53Sor/J |
| 005119 | B6.129S6-Npas2tm1Slm/J |
| 002741 | B6.129S7-Alpltm1Sor/J |
| 005970 | B6.129S7-Atoh1tm2Hzo/J |
| 006039 | B6.129S7-Efnb2tm1And/J |
| 002192 | B6.129S7-Gt(ROSA)26Sor/J |
| 005981 | B6.129S7-Rai1tm1Jrl/J |
| 005039 | B6.129X1-Adra1atm1Pcs/J |
| 005085 | B6.Cg-Cd44tm1Hbg/J |
| 007745 | B6.Cg-Mirn155tm1.1Rsky/J |
| 005317 | B6.Cg-Tg(BAT-lacZ)3Picc/J |
| 006055 | B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J |
| 004178 | B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J |
| 006477 | B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J |
| 003139 | B6.Cg-Tg(DBHn-lacZ)8Rpk/J |
| 006229 | B6.Cg-Tg(DRE-lacZ)2Gswz/J |
| 002982 | B6.Cg-Tg(xstpx-lacZ)32And/J |
| 003504 | B6;129-Gt(ROSA)26Sortm1Sho/J |
| 005064 | B6;129-Slc30a3tm1Rpa/J |
| 005788 | B6;129P2-Cd97tm1Dgen/J |
| 005833 | B6;129P2-Rgs4tm1Dgen/J |
| 002073 | B6;129S-Gt(ROSA)26Sor/J |
| 006470 | B6;129S-Hopxtm1Eno/J |
| 004153 | B6;129S-Mtap7Gt(ROSABetageo)1Sor/J |
| 006958 | B6;129S-Nkd1tm1Kwha/J |
| 006960 | B6;129S-Nkd2tm1Kwha/J |
| 007204 | B6;129S4-2610005L07RikGt(ROSA)73Sor/J |
| 003309 | B6;129S4-Gt(ROSA)26Sortm1Sor/J |
| 004365 | B6;129S6-Srebf1tm1Mbr/J |
| 002317 | B6;129S7-Alpltm1Sor/J |
| 003266 | B6;129S7-Epas1tm1Rus/J |
| 006044 | B6;129S7-Ephb4tm1And/J |
| 003471 | B6;C3H-Tg(CNP-GEO)1Ldh/J |
| 006465 | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J |
| 006680 | B6;CBA-Tg(Olfr16*,taulacZ)19Mom/MomJ |
| 006671 | B6;CBA-Tg(Olfr16*,taulacZ)5Mom/MomJ |
| 006672 | B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ |
| 006673 | B6;CBA-Tg(Olfr16,taulacZ)sn2Mom/MomJ |
| 004141 | B6;CBA-Tg(UAS-lacZ)65Rth/J |
| 002369 | B6;SJL-Tg(c177-lacZ)226Bri/J |
| 002372 | B6;SJL-Tg(c177-lacZ)227Bri/J |
| 002621 | B6;SJL-Tg(tetop-lacZ)2Mam/J |
| 003299 | B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J |
| 002865 | B6CBA-Tg(Wnt1-lacZ)206Amc/J |
| 002955 | C.129S7-Gt(ROSA)26Sor/J |
| 002754 | C57BL/6-Tg(LacZpl)60Vij/J |
| 002193 | C57BL/6J-Tg(MTn-lacZ)204Bri/J |
| 002981 | DBA/2-Tg(xstpx-lacZ)36And/J |
| 004127 | FVB-Tg(Nes-rtTA)306Rvs/J |
| 007225 | FVB.129(B6)-Usp18tm1Dzh/J |
| 008203 | FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)63Ahmb Tg(SMN2)89Ahmb/J |
| 008209 | FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)69Ahmb Tg(SMN2)89Ahmb/J |
| 006214 | FVB.Cg-Smn1tm1Msd/J |
| 005024 | FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd/J |
| 005026 | FVB.Cg-Tg(SMN2)89Ahmb Tg(SMN1*A2G)2023Ahmb Smn1tm1Msd/J |
| 005025 | FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J |
| 003140 | FVB/N-Tg(PAI1-lacZ)1Jjb/J |
| 002856 | FVB/N-Tg(TIE2-lacZ)182Sato/J |
| 005941 | FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J |
| 003315 | FVB/N-Tg(tetORo1-lacZ)3Conk/J |
| 003487 | FVB/NJ-Tg(XGFAP-lacZ)3Mes/J |
| 005878 | NOD.Cg-Cd44tm1Hbg/J |
| 003899 | STOCK Cd44tm1Hbg/J |
| 006241 | STOCK Hhiptm1Amc/J |
| 006578 | STOCK Myoz2tm1Eno/J |
| 005707 | STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J |
| 008212 | STOCK Smn1tm1Msd Tg(Prnp-SMN)92Ahmb Tg(SMN2)89Ahmb/J |
| 006882 | STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J |
| 006850 | STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J |
| 006876 | STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J |
| 006613 | STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J |
| 003919 | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J |
| 003920 | STOCK Tg(CAG-Bgeo/GFP)21Lbe/J |
| 004623 | STOCK Tg(Fos-lacZ)34Efu/J |
| 006674 | STOCK Tg(Olfr16,taulacZ)2030Mom/MomJ |
| 005493 | STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J |
| 002395 | STOCK Tg(Zfy1-lacZ)218Bri/J |
| 003274 | STOCK Tg(tetNZL)2Bjd/J |
| 005728 | STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J |
View lacZ Expression Strains (174 strains)
Strains carrying Tg(CAG-Bgeo,-DsRed*MST)1Nagy allele
View Strains carrying Tg(CAG-Bgeo,-DsRed*MST)1Nagy (1 strain)
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (7 strains)
Strains carrying other alleles of Bgeo
View Strains carrying other alleles of Bgeo (9 strains)
Strains carrying other alleles of DsRed
View Strains carrying other alleles of DsRed (5 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox-System
(loxP-flanked Sequences: Test/Reporter)
Developmental Biology Research
(Cre-lox system)
Fluorescent Proteins
Genetics Research
(Tissue/Cell Markers: Cre-lox system)
lacZ Expression
Bgeo related
Research Tools
lacZ Expression
DsRed related
Fluorescent Proteins
References
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis
40(4):241-6.
[PubMed: 15593332]
[J:109837]
Price and Supply Information
| Strain Name: |
STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J |
| Stock Number: |
005438 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination.
To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page.
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
|
| Licensing | See General Terms and Conditions below
for Licensing and Use Restrictions
|
| Control Information | View Control Information in Strain Details.
|
|---|
General Terms and Conditions
View
JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene
genotyping programs to ensure the genetic background of JAX
® Mice strains as
well as the genotypes of strains with identified molecular mutations.
JAX
® Mice strains are only made available to researchers after meeting our
standards. However, the phenotype of each strain may not be fully
characterized and/or captured in the strain data sheets.
Therefore, we
cannot guarantee a strain's phenotype will meet all expectations. To
ensure that JAX
® Mice will meet the needs of individual research projects
or when requesting a strain that is new to your research, we suggest ordering
and performing tests on a small number of mice to determine suitability for
your particular project.
Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Go to JAX® Mice Query Form