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| These Z/RED transgenic mice express beta-galactosidase (lacZ) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | |||||||||||||||||||
- Description
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-DsRed*MST)1Nagy/J (Changed: 08-MAY-08 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1p (24-JUL-05)
Generation DefinitionsDonating Investigator Dr. Andras Nagy, Mount Sinai Hospital Description
While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.Development
A transgenic construct containing a Red Fluorescent Protein variant (DsRed.MST) gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. ES cell clone containing one copy of the transgene and expressing LacZ was aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross, that were hemizygous for the transgene, were bred to ICR outbred mice. While at The Jackson Laboratory, hemizygous mice are bred together, and may also be bred to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
006051 B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J 008705 B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J 012476 B6;129-Pax7tm2.1(cre/ERT2)Fan/J 006713 B6;129P2-Olfr545tm2Mom/MomJ 008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J 011086 C57BL/6-Tg(Cck-cre)CKres/J 009655 C57BL/6J-Tg(Dcx-DsRed)14Qlu/J 017620 NOD.Cg-Prkdcscid Tg(CAG-DsRed*MST)1Nagy/KupwJ 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J 008241 STOCK Tg(Cspg4-DsRed.T1)1Akik/J 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J 012345 STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Research Tools
lacZ Expression
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-Bgeo,-DsRed*MST)1Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | 11/C1; Tg(ACTB-Bgeo,-DsRed*MST)1Nagy; Tg(ACTB-Bgeo,-DsRed.MST)1Nagy; Tg(CAG-Bgeo,-DsRed*MST)1Nagy; Z/RED; | ||
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| Site of Expression | lacZ is expressed in embryonic and adult tissues; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with Red Fluorescent Protein variant (DsRed.MST) expression | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | ACTB, actin, beta, human | ||
| General Note |
The transgenic ES cell clone 11/C1 was used to generate transgenic mice. The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218). see MMRRC Nagy ES cell lines: ES cell line G4 with DsRed.MST transgene (http://www.mmrrc.org/strains/11988/011988.html)andES cell line G4 with Z/red transgene (http://www.mmrrc.org/strains/11987/011987.html) - dlb | ||
| Molecular Note | The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. [MGI Ref ID J:109837] | ||
| Gene Symbol and Name | Tg(CAG-Bgeo,-DsRed*MST)1Nagy, transgene insertion 1, Andras Nagy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 11/C1; Tg(ACTB-Bgeo,-DsRed*MST)1Nagy; Tg(ACTB-Bgeo,-DsRed.MST)1Nagy; Z/RED; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Bgeo), Standard PCR
Tg(CAG-Bgeo,-DsRed*MST)1Nagy, Melt Curve Analysis
Tg(DsRed), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Additional References
Tg(CAG-Bgeo,-DsRed*MST)1Nagy relatedBoutet SC; Biressi S; Iori K; Natu V; Rando TA. 2010. Taf1 regulates Pax3 protein by monoubiquitination in skeletal muscle progenitors. Mol Cell 40(5):749-61. [PubMed: 21145483] [MGI Ref ID J:168102]
Chai R; Kuo B; Wang T; Liaw EJ; Xia A; Jan TA; Liu Z; Taketo MM; Oghalai JS; Nusse R; Zuo J; Cheng AG. 2012. Wnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea. Proc Natl Acad Sci U S A 109(21):8167-72. [PubMed: 22562792] [MGI Ref ID J:184776]
Humphreys BD; Lin SL; Kobayashi A; Hudson TE; Nowlin BT; Bonventre JV; Valerius MT; McMahon AP; Duffield JS. 2010. Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol 176(1):85-97. [PubMed: 20008127] [MGI Ref ID J:158051]
Humphreys BD; Valerius MT; Kobayashi A; Mugford JW; Soeung S; Duffield JS; McMahon AP; Bonventre JV. 2008. Intrinsic epithelial cells repair the kidney after injury. Cell Stem Cell 2(3):284-91. [PubMed: 18371453] [MGI Ref ID J:148456]
Kataru RP; Kim H; Jang C; Choi DK; Koh BI; Kim M; Gollamudi S; Kim YK; Lee SH; Koh GY. 2011. T lymphocytes negatively regulate lymph node lymphatic vessel formation. Immunity 34(1):96-107. [PubMed: 21256057] [MGI Ref ID J:168062]
Nishijo K; Hosoyama T; Bjornson CR; Schaffer BS; Prajapati SI; Bahadur AN; Hansen MS; Blandford MC; McCleish AT; Rubin BP; Epstein JA; Rando TA; Capecchi MR; Keller C. 2009. Biomarker system for studying muscle, stem cells, and cancer in vivo. FASEB J 23(8):2681-90. [PubMed: 19332644] [MGI Ref ID J:157754]
Phua YL; Martel N; Pennisi DJ; Little MH; Wilkinson L. 2013. Distinct sites of renal fibrosis in Crim1 mutant mice arise from multiple cellular origins. J Pathol 229(5):685-96. [PubMed: 23224993] [MGI Ref ID J:194415]
Qyang Y; Martin-Puig S; Chiravuri M; Chen S; Xu H; Bu L; Jiang X; Lin L; Granger A; Moretti A; Caron L; Wu X; Clarke J; Taketo MM; Laugwitz KL; Moon RT; Gruber P; Evans SM; Ding S; Chien KR. 2007. The renewal and differentiation of Isl1+ cardiovascular progenitors are controlled by a Wnt/beta-catenin pathway. Cell Stem Cell 1(2):165-79. [PubMed: 18371348] [MGI Ref ID J:149713]
Turunen HT; Sipila P; Krutskikh A; Toivanen J; Mankonen H; Hamalainen V; Bjorkgren I; Huhtaniemi I; Poutanen M. 2012. Loss of cysteine-rich secretory protein 4 (Crisp4) leads to deficiency in sperm-zona pellucida interaction in mice. Biol Reprod 86(1):1-8. [PubMed: 21865554] [MGI Ref ID J:185787]
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026] [MGI Ref ID J:137426]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry It has been our experience at The Jackson Laboratory that hemizygotes maintained on a mixed genetic background are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Additionally, smaller mice or mice with improper tooth development may benefit from adding pulverized (and/or increased fat) chow to the cage floor prior to and after weaning to promote the survival of the transgenic pups. Although homozygous animals are born, animals have not survived past 5 weeks of age. Given this, as well as the possibility that fluorescent protein polymer formation may result in DsRed-expressing mice, hemizygous mice are bred to wildtype siblings or to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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