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| These Z/RED transgenic mice express beta-galactosidase (lacZ) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-DsRed*MST)1Nagy/J (Changed: 08-MAY-08 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x F1 (Female x Male) 08-NOV-08 Species laboratory mouse Generation F1 (26-SEP-08)
Generation DefinitionsDonating Investigator Andras Nagy, Mount Sinai Hospital Description
While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.Development
A transgenic construct containing a Red Fluorescent Protein variant (DsRed.MST) gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. ES cell clone containing one copy of the transgene and expressing LacZ was aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross, that were hemizygous for the transgene, were bred to ICR outbred mice. While at The Jackson Laboratory, hemizygous mice are bred together, and may also be bred to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
lacZ Expression
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: Cre-lox System
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Bgeo,-DsRed*MST)1Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | 11/C1; Tg(ACTB-Bgeo,-DsRed*MST)1Nagy; Tg(ACTB-Bgeo,-DsRed.MST)1Nagy; Tg(CAG-Bgeo,-DsRed*MST)1Nagy; Z/RED; | ||
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| Site of Expression | lacZ is expressed in embryonic and adult tissues; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with Red Fluorescent Protein variant (DsRed.MST) expression | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli | ||
| Promoter | ACTB, actin, beta, human | ||
| General Note |
The transgenic ES cell clone 11/C1 was used to generate transgenic mice. The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218). see MMRRC Nagy ES cell lines: ES cell line G4 with DsRed.MST transgene (http://www.mmrrc.org/strains/11988/011988.html)andES cell line G4 with Z/red transgene (http://www.mmrrc.org/strains/11987/011987.html) - dlb | ||
| Molecular Note | The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. [MGI Ref ID J:109837] | ||
| Gene Symbol and Name | Tg(CAG-Bgeo,-DsRed*MST)1Nagy, transgene insertion 1, Andras Nagy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 11/C1; Tg(ACTB-Bgeo,-DsRed*MST)1Nagy; Tg(ACTB-Bgeo,-DsRed.MST)1Nagy; Z/RED; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Bgeo), Standard PCR
Tg(CAG-Bgeo,-DsRed*MST)1Nagy, Melt Curve Analysis
Tg(DsRed), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Additional References
Tg(CAG-Bgeo,-DsRed*MST)1Nagy relatedHumphreys BD; Valerius MT; Kobayashi A; Mugford JW; Soeung S; Duffield JS; McMahon AP; Bonventre JV. 2008. Intrinsic epithelial cells repair the kidney after injury. Cell Stem Cell 2(3):284-91. [PubMed: 18371453] [MGI Ref ID J:148456]
Qyang Y; Martin-Puig S; Chiravuri M; Chen S; Xu H; Bu L; Jiang X; Lin L; Granger A; Moretti A; Caron L; Wu X; Clarke J; Taketo MM; Laugwitz KL; Moon RT; Gruber P; Evans SM; Ding S; Chien KR. 2007. The renewal and differentiation of Isl1+ cardiovascular progenitors are controlled by a Wnt/beta-catenin pathway. Cell Stem Cell 1(2):165-79. [PubMed: 18371348] [MGI Ref ID J:149713]
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026] [MGI Ref ID J:137426]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry It has been our experience at The Jackson Laboratory that hemizygotes maintained on a mixed genetic background are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Additionally, smaller mice or mice with improper tooth development may benefit from adding pulverized (and/or increased fat) chow to the cage floor prior to and after weaning to promote the survival of the transgenic pups. Although homozygous animals are born, animals have not survived past 5 weeks of age. Given this, as well as the possibility that fluorescent protein polymer formation may result in DsRed-expressing mice, hemizygous mice are bred to wildtype siblings or to B6129SF1/J (Stock No. 101043) every few generations to assist in strain viability. Mating System Hemizygote x F1 (Female x Male) 08-NOV-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(CAG-Bgeo,-DsRed*MST)1Nagy
Pairs /Price (US dollars $) Pair Genotype $261.55 Hemizygous for Tg(CAG-Bgeo,-DsRed*MST)1Nagy x B6129SF1/J (101043) Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(CAG-Bgeo,-DsRed*MST)1Nagy
Pairs /Price (US dollars $) Pair Genotype $340.10 Hemizygous for Tg(CAG-Bgeo,-DsRed*MST)1Nagy x B6129SF1/J (101043) Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
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Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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