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| These CCR3 (chemokine (C-C motif) receptor 3) mutant mice may be useful in studies of atopic dermatitis, allergic asthma, increased airway responsiveness/airway hyperresponsiveness (AHR). | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation ?+F2PN1 (29-APR-08) Donating Investigator Craig Gerard, Childrens' Hospital Boston, Harvard MS Description
Mice homozygous for this CCR3 (chemokine (C-C motif) receptor 3) targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RNA protection assay analysis of thymus, spleen and lung tissues. Mice homozygous for the mutation have impaired trafficking of eosinophils. A 7-fold decrease in the number of eosinophils in the small intestine and a 6-fold increase in the spleen is observed. Aerosol allergen challenge does not cause the expected infiltration of eosinophils to the lung tissue and airway lumens (50-70% reduction), although abundant eosinophils are found in the airway blood vessels. Concurrent increases in eosinophils in the spleen and intraepithelial mast cells in the spleen occur after allergen challenge. Homozygotes exhibit increased bronchoconstriction with allergen-induced airway hyperresponsiveness. There is no increase in eosinophils or eosinophil product major basic protein (MBP) in the skin after epicutaneous allergen treatment. Homozygous mice that have been epicutaneously sensitized do not develop increased airway responsiveness, or AHR, following inhalation challenge. These CCR3 mutant mice may be useful in studies of atopic dermatitis, allergic asthma, increased airway responsiveness/airway hyperresponsiveness (AHR).Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes driven by the phosphoglucokinase promoter was used to disrupt a 3kb region containing 39 bp of the region encoding the N-terminal, the start codon and a 5' untranslated region. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to the same for 13 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ccr3tm1Cge/Ccr3tm1Cge
involves: 129S4/SvJae * BALB/c
- immune system phenotype
- abnormal eosinophil physiology (MGI Ref ID J:74509)
- impaired trafficking of eosinophils to the lungs and small intestine
- There is no increase in eosinophils or eosinophil product major basic protein (MBP) in the skin after epicutaneous allergen treatment.
- abnormal mast cell physiology (MGI Ref ID J:75409)
- increased eosinophils in intraepithelial lung tissue after allergen challenge
- respiratory system phenotype
- abnormal airway responsiveness (MGI Ref ID J:75347)
- Epicutaneously sensitized mice do not develop increased airway responsiveness following inhalation challenge.
- increased airway responsiveness (MGI Ref ID J:74509)
- increased bronchoconstriction
This mouse can be used to support research in many areas including:
Dermatology Research
Other
Hematological Research
Immunological Defects
Immunology and Inflammation Research
Autoimmunity
Immunodeficiency (Asthma)
Immunodeficiency (Inflammatory bowel disease)
Inflammation (Asthma)
Inflammation (Inflammatory bowel disease)
Lymphoid Tissue Defects
Research Tools
Dermatology Research
Immunology and Inflammation Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ccr3tm1Cge | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Craig Gerard | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | CCR3 KO; CCR3-; | ||
| Mutation Made By | Craig Gerard, Childrens' Hospital Boston, Harvard MS | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Ccr3, chemokine (C-C motif) receptor 3 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | CC-CKR-3; CC-CKR3; CD193; CKR3; CMKBR3; Cmkbr3; MGC102841; MIP-1 alphaRL2; | ||
| Molecular Note | The gene was disrupted by replacement of a 3 kb region containing the start codon, 39 bp of the N-terminal coding region, and the 5' untranslated region with a PGK-neo cassette via homologous recombination. Absence of gene expression was confirmed by RT-PCR analysis of bone marrow mRNA from homozygous mutant animals. [MGI Ref ID J:74509] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ccr3tm1Cge, MCA, vers. 2
Ccr3tm1Cge, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Humbles AA; Lu B; Friend DS; Okinaga S; Lora J; Al-Garawi A; Martin TR; Gerard NP; Gerard C. 2002. The murine CCR3 receptor regulates both the role of eosinophils and mast cells in allergen-induced airway inflammation and hyperresponsiveness. Proc Natl Acad Sci U S A 99(3):1479-84. [PubMed: 11830666] [MGI Ref ID J:74509]
Additional References
Ccr3tm1Cge relatedAbonia JP; Austen KF; Rollins BJ; Joshi SK; Flavell RA; Kuziel WA; Koni PA; Gurish MF. 2005. Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2. Blood 105(11):4308-13. [PubMed: 15705791] [MGI Ref ID J:98967]
Fischer FR; Luo Y; Luo M; Santambrogio L; Dorf ME. 2001. RANTES-induced chemokine cascade in dendritic cells. J Immunol 167(3):1637-43. [PubMed: 11466387] [MGI Ref ID J:120465]
Fulkerson PC; Fischetti CA; McBride ML; Hassman LM; Hogan SP; Rothenberg ME. 2006. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc Natl Acad Sci U S A 103(44):16418-23. [PubMed: 17060636] [MGI Ref ID J:115273]
Fulkerson PC; Fischetti CA; Rothenberg ME. 2006. Eosinophils and CCR3 regulate interleukin-13 transgene-induced pulmonary remodeling. Am J Pathol 169(6):2117-26. [PubMed: 17148674] [MGI Ref ID J:116219]
Fulkerson PC; Zhu H; Williams DA; Zimmermann N; Rothenberg ME. 2005. CXCL9 inhibits eosinophil responses by a CCR3- and Rac2-dependent mechanism. Blood 106(2):436-43. [PubMed: 15802529] [MGI Ref ID J:107462]
Hallgren J; Jones TG; Abonia JP; Xing W; Humbles A; Austen KF; Gurish MF. 2007. Pulmonary CXCR2 regulates VCAM-1 and antigen-induced recruitment of mast cell progenitors. Proc Natl Acad Sci U S A 104(51):20478-83. [PubMed: 18077323] [MGI Ref ID J:130586]
Ma W; Bryce PJ; Humbles AA; Laouini D; Yalcindag A; Alenius H; Friend DS; Oettgen HC; Gerard C; Geha RS. 2002. CCR3 is essential for skin eosinophilia and airway hyperresponsiveness in a murine model of allergic skin inflammation. J Clin Invest 109(5):621-8. [PubMed: 11877470] [MGI Ref ID J:75347]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Ccr3tm1Cge *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Ccr3tm1Cge x Heterozygous for Ccr3tm1Cge
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Ccr3tm1Cge *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Ccr3tm1Cge x Heterozygous for Ccr3tm1Cge
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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