Strain Name: |
C.129S4-Ccr3tm1Cge/J |
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Stock Number: |
005440 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Ccr3; Ccr3tm1Cge; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Craig Gerard, Childrens' Hospital Boston, Harvard MS Generation ?+F2PN1 (29-APR-08) Strain Description
Mice homozygous for this CCR3 (chemokine (C-C motif) receptor 3) targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RNA protection assay analysis of thymus, spleen and lung tissues. Mice homozygous for the mutation have impaired trafficking of eosinophils. A 7-fold decrease in the number of eosinophils in the small intestine and a 6-fold increase in the spleen is observed. Aerosol allergen challenge does not cause the expected infiltration of eosinophils to the lung tissue and airway lumens (50-70% reduction), although abundant eosinophils are found in the airway blood vessels. Concurrent increases in eosinophils in the spleen and intraepithelial mast cells in the spleen occur after allergen challenge. Homozygotes exhibit increased bronchoconstriction with allergen-induced airway hyperresponsiveness. There is no increase in eosinophils or eosinophil product major basic protein (MBP) in the skin after epicutaneous allergen treatment. Homozygous mice that have been epicutaneously sensitized do not develop increased airway responsiveness, or AHR, following inhalation challenge. These CCR3 mutant mice may be useful in studies of atopic dermatitis, allergic asthma, increased airway responsiveness/airway hyperresponsiveness (AHR).Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes driven by the phosphoglucokinase promoter was used to disrupt a 3kb region containing 39 bp of the region encoding the N-terminal, the start codon and a 5' untranslated region. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to the same for 13 generations.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Ccr3tm1Cge | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Craig Gerard | ||
| Common Name(s) | CCR3 KO; CCR3-; | ||
| Mutation Made By | Craig Gerard, Childrens' Hospital Boston, Harvard MS | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Ccr3, chemokine (C-C motif) receptor 3 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | CC-CKR-3; CC-CKR3; CD193; CKR3; CMKBR3; Cmkbr3; MGC102841; MIP-1 alphaRL2; | ||
| Molecular Note | The gene was disrupted by replacement of a 3 kb region containing the start codon, 39 bp of the N-terminal coding region, and the 5' untranslated region with a PGK-neo cassette via homologous recombination. Absence of gene expression was confirmed by RT-PCR analysis of bone marrow mRNA from homozygous mutant animals. [MGI Ref ID J:74509] | ||
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Ccr3tm1Cge
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Room Number AX11
Dermatology Research
Other
Hematological Research
Immunological Defects
Immunology and Inflammation Research
Autoimmunity
Immunodeficiency (Asthma)
Immunodeficiency (Inflammatory bowel disease)
Inflammation (Asthma)
Inflammation (Inflammatory bowel disease)
Lymphoid Tissue Defects
Research Tools
Dermatology Research
Immunology and Inflammation Research
Selected Reference(s)
Additional ReferencesHumbles AA; Lu B; Friend DS; Okinaga S; Lora J; Al-Garawi A; Martin TR; Gerard NP; Gerard C. 2002. The murine CCR3 receptor regulates both the role of eosinophils and mast cells in allergen-induced airway inflammation and hyperresponsiveness. Proc Natl Acad Sci U S A 99(3):1479-84. [PubMed: 11830666] [MGI Ref ID J:74509]
| Strain Name: | C.129S4-Ccr3tm1Cge/J |
| Stock Number: | 005440 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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