Strain Name: |
STOCK Tg(CAG-DsRed*MST)1Nagy/J |
|---|---|
Stock Number: |
005441 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-DsRed*MST)1Nagy/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; DsRed; Tg(CAG-DsRed*MST)1Nagy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Andras Nagy, Mount Sinai Hospital Generation ?+N1F8 (06-DEC-07) Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.Strain Development
This strain is a derivative of Tg(ACTB-bgeo/DsRed.MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. ES cells containing the (ACTB-DsRed.MST) construct were aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross that were hemizygous for the transgene were bred to ICR outbred mice. This strain is now a mixture of C57BL/6J (Stock No. 000664), 129S6 and random bred ICR strains. Of note, this "DsRed.MST" protein is erroneously designated "DsRed.T3" in the original publication.
Gene & Allele Details
| Allele Symbol | Tg(CAG-DsRed*MST)1Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andras Nagy | ||
| Common Name(s) | Actb-DsRed.T3; DsRed.T3; Tg(ACTB-DsRed*MST)1Nagy; Tg(ACTB-DsRed.MST)1Nagy; | ||
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6)F1 | ||
| Site of Expression | Widespread expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | This strain is a derivative of Tg(ACTB-Bgeo,-DsRed*MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. [MGI Ref ID J:109837] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| This strain is a mixture of C57BL/6J (Stock No. 000664), 129S6 and random bred ICR strains. When breeding hemizygotes, wildtype littermates would be an appropriate control. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(CAG-DsRed*MST)1Nagy QPCR
Tg(DsRed)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygotes or hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
006051 B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
DsRed relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-DsRed*MST)1Nagy/J |
| Stock Number: | 005441 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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