Strain Name:

A.B6 Tyr+-Cybanmf333/J

Stock Number:

005445

Availability:

Cryopreserved - Ready for recovery

Common Names: NMF333;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Coisogenic; Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN4F1+N1p (26-FEB-06)

Description
View strain phenotype and additional information on the Neuroscience Mutagenesis Facility web page for nmf333 entry.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tyr+ allele
000090   129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
005012   A.B6 Tyr+-Myo5ad-l31J/J
002565   A.B6-Tyr+/J
000822   B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J
000899   C.B6-Tyr+ Hbbs/J
000494   J.Cg-Oca2+ Tyr+ Lystbg/J
002281   NFS.C58-Tyr+/J
004304   NOD.CBALs-Tyr+/LtJ
View Strains carrying   Tyr+     (8 strains)

View Strains carrying other alleles of Tyr     (38 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Cybanmf333/Cybanmf333

        A.B6-Tyr+/J
  • behavior/neurological phenotype
  • impaired balance (MGI Ref ID J:132359)
    • mice exhibit a body tilt
    • head tilt (MGI Ref ID J:132359)
      • head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks
  • impaired swimming (MGI Ref ID J:132359)
    • mutant mice are unable to remain at the surface of the water in a forced swim test whereas control mice are good swimmers
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:132359)
    • hearing is unaffected in mutant mice as assessed by ABR analysis
    • absent linear vestibular evoked potential (MGI Ref ID J:132359)
      • encephalographs indicate that mutant mice are nonresponsive even at maximum accelerations
    • absent otoliths (MGI Ref ID J:132359)
      • otoconia are absent in the utricle and saccule of newborn mutant mice
    • head tilt (MGI Ref ID J:132359)
      • head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks
  • immune system phenotype
  • abnormal neutrophil physiology (MGI Ref ID J:132359)
    • mutant neutrophils do not produce superoxide in either the presence or absence of PMA, in contrast to controls
    • Nox activity is completely absent from mutant neutrophils
  • increased inflammatory response (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, spleen derived from mutant mice contain necrotic areas and increased numbers of polymorphonuclear cells within the marginal zone and the red pulp
    • lung inflammation (MGI Ref ID J:132359)
      • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris
  • increased susceptibility to bacterial infection (MGI Ref ID J:132359)
    • mutant animals innoculated intratrachially with 106 CFU of Burkholderia cepacia succumb to the infection within 3 days of infection with animals dying within 24 hours of the first signs of respiratory distress
    • defective bacterial clearance in mutant mice is shown in lung homogenates; at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung homogenates contain more than 108 CFU of bacteria, while lung homogenates from control littermates do not contain any bacteria
  • sepsis (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant spleen contains large numbers of Burkholderia cepacia, whereas controls do not
  • respiratory system phenotype
  • lung inflammation (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cybanmf333 related

Neurobiology Research
Neuroscience Mutagenesis Facility Strain

Sensorineural Research
Vestibular and Hearing Defects

Tyr+ related

Dermatology Research
Color and White Spotting Defects
      oculocutaneous albinism, type I

Mouse/Human Gene Homologs
albinism, tyrosine negative
      wildtype

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Cybanmf333
Allele Name neuroscience mutagenesis facility 333
Allele Type Chemically induced (ENU)
Strain of OriginA.B6-Tyr+/J
Gene Symbol and Name Cyba, cytochrome b-245, alpha polypeptide
Chromosome 8
Gene Common Name(s) b558; cytochrome beta-558; neuroscience mutagenesis facility 333; nmf333; p22 phox; p22-PHOX;
Molecular Note This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein.The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in theneutrophils of control littermates. [MGI Ref ID J:132359]
 
Allele Symbol Tyr+
Allele Name wild type
Allele Type Not Applicable
Gene Symbol and Name Tyr, tyrosinase
Chromosome 7
Gene Common Name(s) C; OCA1A; OCAIA; SHEP3; albino; c; skc35; skin/coat color 35;

Genotyping

Genotyping Information

Genotyping Protocols

Cyba, Pyrosequencing

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Cybanmf333 related

JAX Neuroscience Mutagenesis Facility URL: http://www.jax.org/nmf. 2003. Heritable mouse mutants from JAX NMF ENU Mutagenesis Program MGI Direct Data Submission :.  [MGI Ref ID J:82238]

Nakano Y; Longo-Guess CM; Bergstrom DE; Nauseef WM; Jones SM; Banfi B. 2008. Mutation of the Cyba gene encoding p22 causes vestibular and immune defects in mice. J Clin Invest 118(3):1176-1185. [PubMed: 18292807]  [MGI Ref ID J:132359]

Tyr+ related

Lossie AC; Nakamura H; Thomas SE; Justice MJ. 2005. Mutation of l7Rn3 shows that Odz4 is required for mouse gastrulation. Genetics 169(1):285-99. [PubMed: 15489520]  [MGI Ref ID J:96673]

Miller DA; Dev VG; Tantravahi R; Miller OJ; Schiffman MB; Yates RA; Gluecksohn-Waelsch S. 1974. Cytological detection of the c-25H deletion involving the albino (c) locus on chromosome 7 in the mouse. Genetics 78(3):905-10. [PubMed: 4141683]  [MGI Ref ID J:5520]

Sweet HO. 1987. Acromelanic (c<a>) Mouse News Lett 78:56.  [MGI Ref ID J:14994]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Neuroscience Mutagenesis Facility (NMF).
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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