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Strain Name:

A.B6 Tyr+-Cybanmf333/J

Stock Number:

005445

Availability:

Repository-Cryopreserved

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General Terms and Conditions

Strain Common Names      NMF333;
Genes & Alleles   Cyba;   Cybanmf333;   Tyr;   Tyr+;

Product Information

Strain Details

Type JAX® GEMM® Strain - Chemically Induced Mutation
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Coisogenic
Type JAX® GEMM® Strain - Congenic
Type JAX® GEMM® Strain - Mutant Strain
Specieslaboratory mouse

Strain Description
View strain phenotype and additional information on the Neuroscience Mutagenesis Facility web page for nmf333 entry.

Mammalian Phenotype Terms assigned by genotype

Cybanmf333/Cybanmf333

        A.B6-Tyr+/J
  • behavior/neurological phenotype
  • impaired balance (MGI Ref ID J:132359)
    • mice exhibit a body tilt
    • head tilt (MGI Ref ID J:132359)
      • head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks
  • impaired swimming (MGI Ref ID J:132359)
    • mutant mice are unable to remain at the surface of the water in a forced swim test whereas control mice are good swimmers
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:132359)
    • hearing is unaffected in mutant mice as assessed by ABR analysis
    • absent linear vestibular evoked potential (MGI Ref ID J:132359)
      • encephalographs indicate that mutant mice are nonresponsive even at maximum accelerations
    • absent otoliths (MGI Ref ID J:132359)
      • otoconia are absent in the utricle and saccule of newborn mutant mice
    • head tilt (MGI Ref ID J:132359)
      • head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks
  • immune system phenotype
  • abnormal neutrophil physiology (MGI Ref ID J:132359)
    • mutant neutrophils do not produce superoxide in either the presence or absence of PMA, in contrast to controls
    • Nox activity is completely absent from mutant neutrophils
  • increased inflammatory response (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, spleen derived from mutant mice contain necrotic areas and increased numbers of polymorphonuclear cells within the marginal zone and the red pulp
    • lung inflammation (MGI Ref ID J:132359)
      • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris
  • increased susceptibility to bacterial infection (MGI Ref ID J:132359)
    • mutant animals innoculated intratrachially with 106 CFU of Burkholderia cepacia succumb to the infection within 3 days of infection with animals dying within 24 hours of the first signs of respiratory distress
    • defective bacterial clearance in mutant mice is shown in lung homogenates; at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung homogenates contain more than 108 CFU of bacteria, while lung homogenates from control littermates do not contain any bacteria
  • sepsis (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant spleen contains large numbers of Burkholderia cepacia, whereas controls do not
  • respiratory system phenotype
  • lung inflammation (MGI Ref ID J:132359)
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris

Gene & Allele Details

Allele Symbol Cybanmf333
Allele Name neuroscience mutagenesis facility 333
Strain of OriginA.B6-Tyr+/J
Gene Symbol and Name Cyba, cytochrome b-245, alpha polypeptide
Chromosome 8
Gene Common Name(s) b558; cytochrome beta-558; neuroscience mutagenesis facility 333; nmf333; p22 phox; p22-PHOX;
Molecular Note This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein.The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in theneutrophils of control littermates. [MGI Ref ID J:132359]
 
Allele Symbol Tyr+
Allele Name wild type
Gene Symbol and Name Tyr, tyrosinase
Chromosome 7
Gene Common Name(s) C; OCA1A; OCAIA; SHEP3; albino; c; skc35; skin/coat color 35;

Control Information

  Allele   Control
 Cybanmf333  +/? untyped from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tyr+ allele
000090   129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
005012   A.B6 Tyr+-Myo5ad-l31J/J
002565   A.B6-Tyr+/J
000822   B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J
000899   C.B6-Tyr+ Hbbs/J
000494   J.Cg-Oca2+ Tyr+ Lystbg/J
002281   NFS.C58-Tyr+/J
004304   NOD.CBALs-Tyr+/LtJ
View Strains carrying   Tyr+     (8 strains)

View Strains carrying other alleles of Tyr     (38 strains)

Additional Web Information

Genetic Quality Control Annual Report

Research Applications

This mouse can be used to support research in many areas including:

Cybanmf333 related

Neurobiology Research
Neuroscience Mutagenesis Facility Strain

Sensorineural Research
Vestibular and Hearing Defects

Tyr+ related

Dermatology Research
Color and White Spotting Defects (oculocutaneous albinism, type I)

Mouse/Human Gene Homologs
albinism, tyrosine negative (wildtype)

References

Additional References

Price and Supply Information

Strain Name: A.B6 Tyr+-Cybanmf333/J
Stock Number: 005445

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Neuroscience Mutagenesis Facility (NMF).
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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