Strain Name:

A.B6 Tyr+-Cybanmf333/J

Stock Number:

005445

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Common Names: NMF333;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Coisogenic; Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN4F1+N1pF2p
Generation Definitions

Description
These mutants were detected through routine inspections for overt abnormal behavior; head- and body tilt can be observed at approximately 4 weeks of age (average onset 4.6 +/- 0.6 weeks; n=15). Standard pathology work-up and a whole-mount ear exam on one mutant (56 days of age) revealed no abnormalities. Male or female mutants have been produced and the colony is maintained through regular breeding.

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Development
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tyr+ allele
000090   129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
005012   A.B6 Tyr+-Myo5ad-l31J/J
002565   A.B6-Tyr+/J
000822   B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J
000899   C.B6-Tyr+ Hbbs/J
000494   J.Cg-Oca2+ Tyr+ Lystbg/J
002281   NFS.C58-Tyr+/J
004304   NOD.CBALs-Tyr+/LtJ
View Strains carrying   Tyr+     (8 strains)

Strains carrying other alleles of Tyr
000091   129T1/Sv-Oca2+ Tyrc-ch Dnd1Ter/J
001017   AKXD10/TyJ
000765   AKXD13/TyJ
000954   AKXD15/TyJ
000958   AKXD16/TyJ
001093   AKXD18/TyJ
001062   AKXD21/TyJ
000947   AKXD22/TyJ
000969   AKXD24/TyJ
000777   AKXD6/TyJ
000763   AKXD9/TyJ
000409   B10.129P-H1b Hbbd Tyrc Ea7a/(5M)oSnJ
000418   B10.129P-H1b Tyrc Hbbd/(5M)nSnJ
000432   B10.C-H1b Hbbd Tyrc/(41N)SnJ
000580   B10.D2/nSn-Tyrc-4J/J
000578   B6 x STOCK Tyrc-ch Bmp5se +/+ Myo6sv/J
017614   B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J
000058   B6(Cg)-Tyrc-2J/J
008647   B6.129P2(Cg)-Trpa1tm1.1Kykw Tyrc-2J/J
000383   B6.C-Tyrc H1b Hbbd/ByJ
013590   B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ
003819   B6.Cg-Per2tm1Brd Tyrc-Brd/J
023429   B6.Cg-Tyrc-2J Cdkn1atm1Hpw/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
000035   B6.Cg-Tyrc-J/J
000104   B6.Cg-Tyrc-h/J
005349   B6.Cg-awag Tyrc-2J/GrsrJ
012328   B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J
000054   B6.D2-Tyrc-p/J
023428   B6;129X1-Tyrc-2J Cdkn1atm2Hpw/J
000339   C3H/HeJ-Tyrc-9J/J
001294   C3H/HeJ-Tyrc-a/J
001002   C57BL/10SnJ-Tyrc-11J/J
012257   CB6-Tg(Tyr-TAg)BJjw/Mmjax
001006   CBA/J-Tyrc-10J/J
000657   CE/J
000619   FS/EiJ
004624   FVB.129P2-Pde6b+ Tyrc-ch Fmr1tm1Cgr/J
004828   FVB.129P2-Pde6b+ Tyrc-ch/AntJ
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
000271   SH1/LeJ
001759   STOCK A Tyrc Sha/J
000306   STOCK Dll3pu + Tyrc-ch/+ Oca2p Tyrc-ch/J
018129   STOCK Fah1R Tyrc/RJ
000006   STOCK Hk Tyrc/J
014173   STOCK Omptm1.1(COP4*/EYFP)Tboz/J
000206   STOCK a/a Tyrc-h/J
View Strains carrying other alleles of Tyr     (47 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cytochrome B(-245), Alpha Subunit; CYBA   (CYBA)
Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Negative   (CYBA)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cybanmf333/Cybanmf333

        A.B6 Tyr+-Cybanmf333/J
  • behavior/neurological phenotype
  • head tilt   (MGI Ref ID J:132359)
    • head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks   (MGI Ref ID J:82238)
  • impaired balance
    • mice exhibit a body tilt   (MGI Ref ID J:132359)
  • impaired swimming
    • mutant mice are unable to remain at the surface of the water in a forced swim test whereas control mice are good swimmers   (MGI Ref ID J:132359)
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype
    • hearing is unaffected in mutant mice as assessed by ABR analysis   (MGI Ref ID J:132359)
    • absent linear vestibular evoked potential
      • encephalographs indicate that mutant mice are nonresponsive even at maximum accelerations   (MGI Ref ID J:132359)
    • absent otoliths
      • otoconia are absent in the utricle and saccule of newborn mutant mice   (MGI Ref ID J:132359)
  • immune system phenotype
  • abnormal neutrophil physiology
    • mutant neutrophils do not produce superoxide in either the presence or absence of PMA, in contrast to controls   (MGI Ref ID J:132359)
    • Nox activity is completely absent from mutant neutrophils   (MGI Ref ID J:132359)
  • increased inflammatory response
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, spleen derived from mutant mice contain necrotic areas and increased numbers of polymorphonuclear cells within the marginal zone and the red pulp   (MGI Ref ID J:132359)
    • lung inflammation
      • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris   (MGI Ref ID J:132359)
  • increased susceptibility to bacterial infection
    • mutant animals innoculated intratrachially with 106 CFU of Burkholderia cepacia succumb to the infection within 3 days of infection with animals dying within 24 hours of the first signs of respiratory distress   (MGI Ref ID J:132359)
    • defective bacterial clearance in mutant mice is shown in lung homogenates; at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung homogenates contain more than 108 CFU of bacteria, while lung homogenates from control littermates do not contain any bacteria   (MGI Ref ID J:132359)
  • sepsis
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant spleen contains large numbers of Burkholderia cepacia, whereas controls do not   (MGI Ref ID J:132359)
  • respiratory system phenotype
  • lung inflammation
    • at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris   (MGI Ref ID J:132359)
  • hematopoietic system phenotype
  • abnormal neutrophil physiology
    • mutant neutrophils do not produce superoxide in either the presence or absence of PMA, in contrast to controls   (MGI Ref ID J:132359)
    • Nox activity is completely absent from mutant neutrophils   (MGI Ref ID J:132359)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cybanmf333 related

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      Neutrophil Defects

Neurobiology Research
Neuroscience Mutagenesis Facility Strain
Vestibular Defects

Sensorineural Research
Vestibular Defects

Tyr+ related

Dermatology Research
Color and White Spotting Defects
      oculocutaneous albinism, type I

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cybanmf333
Allele Name neuroscience mutagenesis facility 333
Allele Type Chemically induced (ENU)
Common Name(s) p22(phox)-; p22phox-;
Strain of OriginA.B6-Tyr+/J
Gene Symbol and Name Cyba, cytochrome b-245, alpha polypeptide
Chromosome 8
Gene Common Name(s) b558; cytochrome beta-558; neuroscience mutagenesis facility 333; nmf333; p22 phox; p22-PHOX;
Molecular Note This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein.The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in theneutrophils of control littermates. [MGI Ref ID J:132359]
 
Allele Symbol Tyr+
Allele Name wild type
Allele Type Not Applicable
Gene Symbol and Name Tyr, tyrosinase
Chromosome 7
Gene Common Name(s) ATN; C; CMM8; OCA1; OCA1A; OCAIA; SHEP3; albino; c; skc35; skin/coat color 35;

Genotyping

Genotyping Information

Genotyping Protocols

Cyba, Pyrosequencing


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Cybanmf333 related

Bauernfeind F; Bartok E; Rieger A; Franchi L; Nunez G; Hornung V. 2011. Cutting edge: reactive oxygen species inhibitors block priming, but not activation, of the NLRP3 inflammasome. J Immunol 187(2):613-7. [PubMed: 21677136]  [MGI Ref ID J:178032]

Bulua AC; Simon A; Maddipati R; Pelletier M; Park H; Kim KY; Sack MN; Kastner DL; Siegel RM. 2011. Mitochondrial reactive oxygen species promote production of proinflammatory cytokines and are elevated in TNFR1-associated periodic syndrome (TRAPS). J Exp Med 208(3):519-33. [PubMed: 21282379]  [MGI Ref ID J:176847]

JAX Neuroscience Mutagenesis Facility. 2003. Heritable mouse mutants from JAX NMF ENU Mutagenesis Program MGI Direct Data Submission :.  [MGI Ref ID J:82238]

Nakano Y; Longo-Guess CM; Bergstrom DE; Nauseef WM; Jones SM; Banfi B. 2008. Mutation of the Cyba gene encoding p22 causes vestibular and immune defects in mice. J Clin Invest 118(3):1176-1185. [PubMed: 18292807]  [MGI Ref ID J:132359]

Patel KK; Miyoshi H; Beatty WL; Head RD; Malvin NP; Cadwell K; Guan JL; Saitoh T; Akira S; Seglen PO; Dinauer MC; Virgin HW; Stappenbeck TS. 2013. Autophagy proteins control goblet cell function by potentiating reactive oxygen species production. EMBO J 32(24):3130-44. [PubMed: 24185898]  [MGI Ref ID J:206181]

Spencer NY; Yan Z; Boudreau RL; Zhang Y; Luo M; Li Q; Tian X; Shah AM; Davisson RL; Davidson B; Banfi B; Engelhardt JF. 2011. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 286(11):8977-87. [PubMed: 21212270]  [MGI Ref ID J:170529]

Tyr+ related

Erickson RP; Gluecksohn-Waelsch S; Cori CF. 1968. Glucose-6-phosphatase deficiency caused by radiation-induced alleles at the albino locus in the mouse. Proc Natl Acad Sci U S A 59(2):437-44. [PubMed: 4296364]  [MGI Ref ID J:5063]

Lossie AC; Nakamura H; Thomas SE; Justice MJ. 2005. Mutation of l7Rn3 shows that Odz4 is required for mouse gastrulation. Genetics 169(1):285-99. [PubMed: 15489520]  [MGI Ref ID J:96673]

Miller DA; Dev VG; Tantravahi R; Miller OJ; Schiffman MB; Yates RA; Gluecksohn-Waelsch S. 1974. Cytological detection of the c-25H deletion involving the albino (c) locus on chromosome 7 in the mouse. Genetics 78(3):905-10. [PubMed: 4141683]  [MGI Ref ID J:5520]

Oak Ridge National Laboratory. 2005. Information obtained from the Oak Ridge National Laboratory Mutant Mouse Database (ORNL), Oak Ridge, TN Unpublished :.  [MGI Ref ID J:100221]

Sweet HO. 1987. Acromelanic (c<a>) Mouse News Lett 78:56.  [MGI Ref ID J:14994]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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