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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Chemically Induced Mutation; Coisogenic; Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N4F1+N1pF2p
Generation DefinitionsDescription
View strain phenotype and additional information on the Neuroscience Mutagenesis Facility web page for nmf333 entry.
Control Information
| Control | ||
|---|---|---|
| +/? untyped from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tyr+ allele
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 005012 A.B6 Tyr+-Myo5ad-l31J/J 002565 A.B6-Tyr+/J 000822 B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J 000899 C.B6-Tyr+ Hbbs/J 000494 J.Cg-Oca2+ Tyr+ Lystbg/J 002281 NFS.C58-Tyr+/J 004304 NOD.CBALs-Tyr+/LtJ View Strains carrying Tyr+ (8 strains)
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
Cybanmf333/Cybanmf333
A.B6 Tyr+-Cybanmf333/J
- behavior/neurological phenotype
- head tilt (MGI Ref ID J:132359)
- head- and body tilt can be observed at approximately 4 weeks of age with average onset 4.6 +/- 0.6 weeks (MGI Ref ID J:82238)
- impaired balance
- mice exhibit a body tilt (MGI Ref ID J:132359)
- impaired swimming
- mutant mice are unable to remain at the surface of the water in a forced swim test whereas control mice are good swimmers (MGI Ref ID J:132359)
- hearing/vestibular/ear phenotype
- *normal* hearing/vestibular/ear phenotype
- hearing is unaffected in mutant mice as assessed by ABR analysis (MGI Ref ID J:132359)
- immune system phenotype
- abnormal neutrophil physiology
- increased inflammatory response
- at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, spleen derived from mutant mice contain necrotic areas and increased numbers of polymorphonuclear cells within the marginal zone and the red pulp (MGI Ref ID J:132359)
- lung inflammation
- at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris (MGI Ref ID J:132359)
- increased susceptibility to bacterial infection
- mutant animals innoculated intratrachially with 106 CFU of Burkholderia cepacia succumb to the infection within 3 days of infection with animals dying within 24 hours of the first signs of respiratory distress (MGI Ref ID J:132359)
- defective bacterial clearance in mutant mice is shown in lung homogenates; at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung homogenates contain more than 108 CFU of bacteria, while lung homogenates from control littermates do not contain any bacteria (MGI Ref ID J:132359)
- sepsis
- at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant spleen contains large numbers of Burkholderia cepacia, whereas controls do not (MGI Ref ID J:132359)
- respiratory system phenotype
- lung inflammation
- at 3.5 days post-infection with 104 CFU of Burkholderia cepacia, mutant lung tissue is damaged by necrotizing pneumonia and alveolar spaces are filled with granulocytes, macrophages and cell debris (MGI Ref ID J:132359)
This mouse can be used to support research in many areas including:Cybanmf333 related
Tyr+ relatedImmunology and Inflammation Research
Immunodeficiency
Neutrophil Defects
Neurobiology Research
Neuroscience Mutagenesis Facility Strain
Vestibular Defects
Sensorineural Research
Vestibular Defects
Dermatology Research
Color and White Spotting Defects
oculocutaneous albinism, type I
Mouse/Human Gene Homologs
albinism, tyrosine negative
wildtype
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cybanmf333 | ||
|---|---|---|---|
| Allele Name | neuroscience mutagenesis facility 333 | ||
| Allele Type | Chemically induced (ENU) | ||
| Strain of Origin | A.B6-Tyr+/J | ||
| Gene Symbol and Name | Cyba, cytochrome b-245, alpha polypeptide | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | b558; cytochrome beta-558; neuroscience mutagenesis facility 333; nmf333; p22 phox; p22-PHOX; | ||
| Molecular Note | This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein.The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in theneutrophils of control littermates. [MGI Ref ID J:132359] | ||
| Allele Symbol | Tyr+ | ||
| Allele Name | wild type | ||
| Allele Type | Not Applicable | ||
| Gene Symbol and Name | Tyr, tyrosinase | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | C; CMM8; OCA1A; OCAIA; SHEP3; albino; c; skc35; skin/coat color 35; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cyba, Pyrosequencing
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Cybanmf333 relatedTyr+ relatedBauernfeind F; Bartok E; Rieger A; Franchi L; Nunez G; Hornung V. 2011. Cutting edge: reactive oxygen species inhibitors block priming, but not activation, of the NLRP3 inflammasome. J Immunol 187(2):613-7. [PubMed: 21677136] [MGI Ref ID J:178032]
Bulua AC; Simon A; Maddipati R; Pelletier M; Park H; Kim KY; Sack MN; Kastner DL; Siegel RM. 2011. Mitochondrial reactive oxygen species promote production of proinflammatory cytokines and are elevated in TNFR1-associated periodic syndrome (TRAPS). J Exp Med 208(3):519-33. [PubMed: 21282379] [MGI Ref ID J:176847]
JAX Neuroscience Mutagenesis Facility. 2003. Heritable mouse mutants from JAX NMF ENU Mutagenesis Program MGI Direct Data Submission :. [MGI Ref ID J:82238]
Nakano Y; Longo-Guess CM; Bergstrom DE; Nauseef WM; Jones SM; Banfi B. 2008. Mutation of the Cyba gene encoding p22 causes vestibular and immune defects in mice. J Clin Invest 118(3):1176-1185. [PubMed: 18292807] [MGI Ref ID J:132359]
Spencer NY; Yan Z; Boudreau RL; Zhang Y; Luo M; Li Q; Tian X; Shah AM; Davisson RL; Davidson B; Banfi B; Engelhardt JF. 2011. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 286(11):8977-87. [PubMed: 21212270] [MGI Ref ID J:170529]
Erickson RP; Gluecksohn-Waelsch S; Cori CF. 1968. Glucose-6-phosphatase deficiency caused by radiation-induced alleles at the albino locus in the mouse. Proc Natl Acad Sci U S A 59(2):437-44. [PubMed: 4296364] [MGI Ref ID J:5063]
Lossie AC; Nakamura H; Thomas SE; Justice MJ. 2005. Mutation of l7Rn3 shows that Odz4 is required for mouse gastrulation. Genetics 169(1):285-99. [PubMed: 15489520] [MGI Ref ID J:96673]
Miller DA; Dev VG; Tantravahi R; Miller OJ; Schiffman MB; Yates RA; Gluecksohn-Waelsch S. 1974. Cytological detection of the c-25H deletion involving the albino (c) locus on chromosome 7 in the mouse. Genetics 78(3):905-10. [PubMed: 4141683] [MGI Ref ID J:5520]
Oak Ridge National Laboratory. 2005. Information obtained from the Oak Ridge National Laboratory Mutant Mouse Database (ORNL), Oak Ridge, TN Unpublished :. [MGI Ref ID J:100221]
Sweet HO. 1987. Acromelanic (c<a>) Mouse News Lett 78:56. [MGI Ref ID J:14994]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Neuroscience Mutagenesis Facility (NMF).
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| +/? untyped from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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