Strain Name: |
129-Tg(CAG-EYFP)7AC5Nagy/J |
|---|---|
Stock Number: |
005483 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
129-Tg(ACTB-EYFP)7AC5Nagy/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; YFP; Tg(CAG-EYFP)7AC5Nagy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Andras Nagy, Mount Sinai Hospital Strain Description
Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the identical pCX::EYFP vector. Although the transgene integrated independently in the two founder lines, the donating investigators report that mice derived from lines YC5/EYFP and 7AC5/EYFP are "phenotypically indistinguishable."
Strain Development
A transgenic construct (pCX::EYFP) containing an Enhanced Yellow Fluorescent Protein gene (ClonTech) under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Many different ES cell clones were identified containing the construct (including line YC5/EYFP and 7AC5/EYFP). For these mice, 7AC5/EYFP clones were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females to test for germline transmission. Chimeric mice found to be capable of germline transmission were themselves bred to 129S1 females. The transgenic offspring were intercrossed to generate homozygotes. These mice, derived from founder line 7AC5/EYFP ("phenotypically indistinguishable" from the originally published YC5/EYFP founder line), were sent by the Dr. Andras Nagy research group at Mount Sinai Hospital to The Jackson Laboratory in 2005.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(CAG-EYFP)7AC5Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 7AC5, Andras Nagy | ||
| Common Name(s) | 7AC5/EYFP; Tg(ACTB-EYFP)7AC5Nagy; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Widespread expression with the noted exception of adipocytes and erythrocytes. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The transgene construct is composed of an enhanced yellow fluorescent protein (EYFP) under control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. [J:64336] | ||
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
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Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesHadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497] [J:93696]
Price and Supply Information
| Strain Name: | 129-Tg(CAG-EYFP)7AC5Nagy/J |
| Stock Number: | 005483 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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