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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129-Tg(ACTB-EYFP)7AC5Nagy/J (Changed: 08-MAY-08 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Andras Nagy, Mount Sinai Hospital Description
Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the identical pCX::EYFP vector. Although the transgene integrated independently in the two founder lines, the donating investigators report that mice derived from lines YC5/EYFP and 7AC5/EYFP are "phenotypically indistinguishable."
Development
A transgenic construct (pCX::EYFP) containing an Enhanced Yellow Fluorescent Protein gene (ClonTech) under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Many different ES cell clones were identified containing the construct (including line YC5/EYFP and 7AC5/EYFP). For these mice, 7AC5/EYFP clones were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females to test for germline transmission. Chimeric mice found to be capable of germline transmission were themselves bred to 129S1 females. The transgenic offspring were intercrossed to generate homozygotes. These mice, derived from founder line 7AC5/EYFP ("phenotypically indistinguishable" from the originally published YC5/EYFP founder line), were sent by the Dr. Andras Nagy research group at Mount Sinai Hospital to The Jackson Laboratory in 2005.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(CAG-EYFP)7AC5Nagy/Tg(CAG-EYFP)7AC5Nagy
involves: 129S1/Sv * 129X1/SvJThis mouse can be used to support research in many areas including:
YFP relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-EYFP)7AC5Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 7AC5, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | 7AC5/EYFP; Tg(ACTB-EYFP)7AC5Nagy; Tg(CAG-EYFP)7AC5Nagy; | ||
| Mutation Made By | Dr. Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Widespread expression with the noted exception of adipocytes and erythrocytes. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The transgene construct is composed of an enhanced yellow fluorescent protein (EYFP) under control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. [MGI Ref ID J:64336] | ||
| Gene Symbol and Name | Tg(CAG-EYFP)7AC5Nagy, transgene insertion 7AC5, Andras Nagy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 7AC5/EYFP; Tg(ACTB-EYFP)7AC5Nagy; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497] [MGI Ref ID J:93696]
Additional References
Hadjantonakis AK; Nagy A. 2000. FACS for the isolation of individual cells from transgenic mice harboring a fluorescent protein reporter Genesis 27(3):95-8. [PubMed: 10951501] [MGI Ref ID J:64336]
Tg(CAG-EYFP)7AC5Nagy relatedBianco C; Cotten C; Lonardo E; Strizzi L; Baraty C; Mancino M; Gonzales M; Watanabe K; Nagaoka T; Berry C; Arai AE; Minchiotti G; Salomon DS. 2009. Cripto-1 is required for hypoxia to induce cardiac differentiation of mouse embryonic stem cells. Am J Pathol 175(5):2146-58. [PubMed: 19834060] [MGI Ref ID J:154688]
Chiu LL; Montgomery M; Liang Y; Liu H; Radisic M. 2012. Perfusable branching microvessel bed for vascularization of engineered tissues. Proc Natl Acad Sci U S A 109(50):E3414-23. [PubMed: 23184971] [MGI Ref ID J:193134]
Cox BJ; Vollmer M; Tamplin O; Lu M; Biechele S; Gertsenstein M; van Campenhout C; Floss T; Kuhn R; Wurst W; Lickert H; Rossant J. 2010. Phenotypic annotation of the mouse X chromosome. Genome Res 20(8):1154-64. [PubMed: 20548051] [MGI Ref ID J:163340]
Hadjantonakis AK; Nagy A. 2000. FACS for the isolation of individual cells from transgenic mice harboring a fluorescent protein reporter Genesis 27(3):95-8. [PubMed: 10951501] [MGI Ref ID J:64336]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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