Strain Name: |
B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J |
|---|---|
Stock Number: |
005491 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6.Cg-Mapttm1(GFP)Klt Tg(MAPT)8cPdav/J (Changed: 14-APR-06
) |
| Strain Common Names | htau; |
| Genes & Alleles | MAPT; Mapt; Mapttm1(EGFP)Klt; Tg(MAPT)8cPdav; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Karen Duff and Peter Davies, Nathan Kline Inst & Albert Einstein Coll Generation N9+1F5 (31-DEC-07) Strain Description
Mice that are homozygous for the targeted allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by 3 months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as 2 months of age. These mutant mice may be useful in studies examining the relationship between human MAPT and Alzheimer's disease pathogenesisIn an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
Double mutant mice were generated by crossing transgenic mice (mouse line 8C) with Mapt targeted mutant mice. The targeted mutant allele was created by inserting EGFP coding sequence into the first Mapt exon, disrupting expression of the Mapt gene and producing a cytoplasmic EGFP protein fused to the first 31 MAPT amino acids (see Stock Number 004779). The transgenic allele consists of a PAC insert of 200-250 kb that includes the coding sequence, intronic regions and regulatory elements of the human MAPT gene. The targeted allele was created in 129S4/SvJae-derived J1 embryonic stem cells which were subsequently injected into C57BL/6 blastocysts. The transgenic allele was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males. Mice containing both alleles were crossed to C57BL/6 mice for nine generations by the donating investigator. Upon arrival at The Jackson Laboratory, the mice were crossed once more to C57BL/6 (N10). Offspring were mated to generate mice that are homozygous at the targeted mutant allele.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Mapttm1(EGFP)Klt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kerry Lee Tucker | ||
| Common Name(s) | Mapttm1(GFP)Klt; tau ko; | ||
| Mutation Made By | Kerry Tucker, Ruprecht Karls University of Heidelberg | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MGC156663; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette encoding EGFP and neo. Fusion protein including GFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene. [J:66561] | ||
| Allele Symbol | Tg(MAPT)8cPdav | ||
| Allele Name | transgene insertion 8c, Peter Davies | ||
| Common Name(s) | 8c; | ||
| Mutation Made By | Peter Davies, Albert Einstein College of Medicine | ||
| Strain of Origin | Swiss Webster x (C57BL/6 x DBA)F1 | ||
| Site of Expression | Neurons: EGFP signal observed at 9.0 dpc (days post-coitum) in the trilgeminal ganglion. At 10.75 dpc. EGFP was seen throughout the developing nervous system. | ||
| Expressed Gene | MAPT, microtubule-associated protein tau, human | ||
| Promoter | MAPT, microtubule-associated protein tau, human | ||
| General Note | Mice that are homozygous for the Mapttm1(GFP)Klt allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by 3 months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as 2months of age. | ||
| Molecular Note | The transgene contains the coding sequence, intronic regions, and regulatory elements of the human microtubule-associated protein tau (MAPT) gene. [J:85952] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Mice from the colony that are homozygous for the targeted mutation and noncarrier for the transgene should be considered for use as controls. The most appropriate control animal will depend on the nature of the experiment. | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Mapttm1GFPklt
Tg(MAPT)8cPdav
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, mice that are homozygous for the targeted mutation and hemizygous for the transgene are bred with mice homozygous for the targeted mutation and wildtype for the transgene. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004779 STOCK Mapttm1(EGFP)Klt/J
004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
008169 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J 003741 B6D2-Tg(Prnp-MAPT)43Vle/J
007251 B6.129-Mapttm1Hnd/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
MAPT relatedNeurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Mapttm1(EGFP)Klt relatedNeurobiology Research
Alzheimer's Disease
Parkinson's Disease
Tg(MAPT)8cPdav relatedNeurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)
Alzheimer's Disease (Tau (Mapt) mutants)
References
Selected Reference(s)
Additional ReferencesAndorfer C; Kress Y; Espinoza M; de Silva R; Tucker KL; Barde YA; Duff K; Davies P. 2003. Hyperphosphorylation and aggregation of tau in mice expressing normal human tau isoforms. J Neurochem 86(3):582-90. [PubMed: 12859672] [J:84638]
Price and Supply Information
| Strain Name: | B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J |
| Stock Number: | 005491 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $298.70 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav | |||
| Individual Mouse Price | $298.70 | Homozygous for Mapttm1(EGFP)Klt, Noncarrier | |||
| Pair | $597.30 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav x Homozygous for Mapttm1(EGFP)Klt, Noncarrier | |||
Pricing for aged mice
| Price(s) in US dollars ($) | Genotype(s) Provided | |||
|---|---|---|---|---|
| Base Price 6 months (never bred) | $426.10 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav | ||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. For Aged Mice: Mice aged six months and older and retired breeders are available in small quantities, please contact JAX® Services for current availability and to place an order. Please note that the standard supply level listed above does not apply to the aged mice. Orders for aged mice will be processed solely through JAX® Services. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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