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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator James N Topper, Frazier Healthcare Ventures Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice harbor co-injected transgenic constructs. The reverse tetracycline-controlled transactivator (rtTA) protein is expressed under the direction of the endothelial-specific receptor tyrosine kinase enhancer/promoter (Tek). The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene (lacZ) under the control of a tetracycline-responsive element (TRE). In the presence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of lacZ is observed in the nuclei of vascular endothelium in a wide variety of tissues (aorta, heart, brain, lung, kidney, liver, spleen, uterus, prostate, stomach, skeletal muscle, large and small intestine). Expression is observed as early as embryonic day 9.5. In the absence of tetracycline, some lacZ expression occurs in the smaller branches of the aorta. Additionally, the donating investigators report a trend towards diminished lacZ expression when the alleles are crossed onto a C57BL/6 background. This strain provides a tool that allows the inducible expression of genes in the vascular endothelium during early developmental stages and in the adult.This strain is on a genetic background different from that on which the allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
To generate this transgenic line, two transgenic constructs were co-injected into B6CBA fertilized oocytes. The first construct included a 2.1 kb endothelial-specific receptor tyrosine kinase (Tek) promoter fragment, the Tet-on transactivator (tTA), SV40 polyadenylation sequence and a 1.7 kb enhancer sequence derived from Tek intron 1. The other construct consisted of a tetracycline-responsive element, nuclear-localizing beta-galactosidase (lacZ) gene and a SV40 polyadenylation sequence. Although the transgenic constructs are closely linked in this transgenic line (1425), the donating investigators report that no definitive evidence exits indicating that they have cointegrated. The mice were later crossed to Apoe mutant mice (that may have been congenic on the C57BL/6 background). During the development of the colony at The Jackson Laboratory, mice were selected for the (Tek-rtTA,TRE-lacZ) transgene and against the Apoe mutant allele.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
lacZ Expression
Cancer Research
Tetop Tet System
endothelial cell marker for neovascularization
Cardiovascular Research
Tetop Tet System
endothelial cell marker (lacZ) for neovascularization
endothelial cell marker for neovascularization
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: endothelial cell markers for neovascularization
Tissue/Cell Markers: endothelial cells
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tek-rtTA,TRE-lacZ)1425Tpr | ||
|---|---|---|---|
| Allele Name | transgene insertion 1425, James N Topper | ||
| Allele Type | Transgenic (Reporter) | ||
| Mutation Made By | Peter Teng, Millennium Pharmaceuticals | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | Expresses rtTA in vascular endothelial cells. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Tek, TEK tyrosine kinase, endothelial, rat | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the presence of tetracycline or a tetracycline analog such as doxycycline, selective expression of beta-galactosidase isobserved in the nuclei of vascular endothelium in a wide variety of tissues: aorta, heart, brain, lung, kidney, liver, spleen, uterus, prostate, stomach, skeletal muscle, large and small intestine. Expression is observed as early as embryonic day 9.5. Inthe absence of tetracycline, some beta-galactosidase expression occurs in the smaller branches of the aorta. A trend towards diminished beta-galactosidase expression when the alleles are crossed onto a C57BL/6 background has been reported. | ||
| Molecular Note | Two transgenic constructs were co-injected into B6CBA fertilized oocytes. The first construct included a 2.1 kb endothelial-specific receptor tyrosine kinase (Tek) promoter fragment, the Tet-on transactivator, SV40 polyadenylation sequence and a 1.7 kb enhancer sequence derived from Tek intron 1. The other construct consisted of a tetracycline-responsive element, nuclear-localizing beta-galactosidase gene and a SV40 polyadenylation sequence. Although the transgenic constructs are closely linked in transgenic line 1425, no definitive evidence exits indicating that they have cointegrated. [MGI Ref ID J:83046] | ||
| Gene Symbol and Name | Tg(Tek-rtTA,TRE-lacZ)1425Tpr, transgene insertion 1425, James N Topper | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ, Standard PCR
Tg(tTA), Melt Curve Analysis
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Teng PI; Dichiara MR; Komuves LG; Abe K; Quertermous T; Topper JN. 2002. Inducible and selective transgene expression in murine vascular endothelium. Physiol Genomics 11(2):99-107. [PubMed: 12388791] [MGI Ref ID J:83046]
Additional References
Tg(Tek-rtTA,TRE-lacZ)1425Tpr relatedKirov A; Duarte M; Guay J; Karolak M; Yan C; Oxburgh L; Prudovsky I. 2012. Transgenic expression of nonclassically secreted FGF suppresses kidney repair. PLoS One 7(5):e36485. [PubMed: 22606265] [MGI Ref ID J:187244]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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