Strain Name: |
NOD-Tg(Ins2*Y16A)1Ell/GseJ |
|---|---|
Stock Number: |
005522 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Ins2; Tg(Ins2*Y16A)1Ell; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator George Eisenbarth, U of Colorado Generation N1F13+N1p (09-NOV-05) Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Expression has been reported in the pancreatic islets and thymus of NOD mice carrying the Tg(Ins2*Y16A)1Ell mutation. Approximately 75% of line B female transgenic mice become diabetic in the presence of native insulin genes by 35 weeks of age. NOD female transgenic mice lacking both Ins1 and Ins2 fail to produce insulin autoantibodies, and there is no diabetes or insulitis at 26 weeks of age, but sialitis is present. Donating investigator reports male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes before 10 weeks of age. In contrast, transgenic female mice in the presence of Ins1 and lacking Ins2 develop diabetes in 75% of the animals by 25 weeks of age (Nakayama et al, 2004, 2005). Donating investigator reports line B transgenics have lower expression levels than line F and that male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes before 10 weeks of age.NOD-Tg(Ins2*Y16A)1Ell/GseJ is useful to study insulin-reactive autoimmunity.
Strain Development
NOD-Tg(Ins2*Y16A)1Ell/GseJ expresses the rat insulin 7 promoter fused to a mutated preproinsulin II cDNA, where the tyrosine at position 16 of the b chain was replaced with alanine. This transgene was inserted by Nakayama et al, 2004 directly into NOD/MrkTac oocytes. Founder line B has been maintained on the NOD background. In 2005, The Jackson Laboratory received NOD-Tg(Ins2*Y16A)1Ell/GseJ at generation N3.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2*Y16A)1Ell | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, John F Elliot | ||
| Common Name(s) | tg B:16 alanine, line B; | ||
| Mutation Made By | John Elliott, University of Alberta | ||
| Strain of Origin | NOD/MrkTac | ||
| Expressed Gene | Ins2, insulin 2, rat | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | This transgenic construct contains the rat insulin7 construct of the rat insulin 2 gene fused to a mutated preproinsulin II cDNA where the tyrosine at position 16 of the B chain was replaced with alanine. The transgene is expressed in pancreatic beta cells. [MGI Ref ID J:97755] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2*Y16A)
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying Tg(Ins2*Y16A)1Ell allele
005524 NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ View Strains carrying Tg(Ins2*Y16A)1Ell (1 strain)
Additional Web Information
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (autoimmune pancreatitis and sialoadenitis)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
References
Selected Reference(s)
Additional ReferencesNakayama M; Moriyama H; Abiru N; Babu SR; Sikora K; Li M; Miao D; Hutton JC; Elliott JF; Eisenbarth GS. 2004. Establishment of native insulin-negative NOD mice and the methodology to distinguish specific insulin knockout genotypes and a B:16 alanine preproinsulin transgene. Ann N Y Acad Sci 1037:193-8. [PubMed: 15699516] [MGI Ref ID J:97755]
Price and Supply Information
| Strain Name: | NOD-Tg(Ins2*Y16A)1Ell/GseJ |
| Stock Number: | 005522 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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