Strain Name: |
NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ |
|---|---|
Stock Number: |
005524 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Ins1; Ins1tm1Jja; Ins2; Ins2tm1Jja; Tg(Ins2*Y16A)1Ell; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/MrkTac Donor Strain D3 (129S2/SvPas) Donating Investigator George Eisenbarth, U of Colorado H2 Haplotype g7 Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Transgenic mice reportedly express the mutant Ins2*Y16A protein in the pancreatic islets and thymus. The donating investigator reports approximately 75% of female transgenic mice (founder line B) become diabetic in the presence of native insulin genes by 35 weeks of age. In contrast, NOD female transgenic mice lacking both Ins1 and Ins2 fail to produce insulin autoantibodies, and neither diabetes nor insulitis develops by 26 weeks of age. Sialitis does occur, however. Line B transgenics have lower expression levels than line F (see Stock No. 005525), and 50% of the line B male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes, with little to no insulitis, before 10 weeks of age.This strain is useful to study insulin-reactive autoimmunity.
Strain Development
In the transgenic construct the rat insulin 7 promoter is fused to a mutated preproinsulin II cDNA encoding a tyrosine to alanine amino acid substitution at position 16 of the b chain. This transgene was microinjected directly into NOD/MrkTac oocytes (Nakayama et al, 2004). Offspring from the founder line B was further crossed to NOD.Ins1-/- and NOD.Ins2-/- to generate this double mutant, transgenic strain In 2006, the T1DR received the double mutant transgenic strain at generation N10F7.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Ins1tm1Jja | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jacques Jami | ||
| Common Name(s) | Ins1-; | ||
| Mutation Made By | Jacques Jami, INSERM | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Ins1, insulin I | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | Ins-1; Ins2-rs1; insulin 2, related sequence 1; insulin I or insulin pseudogene; | ||
| Molecular Note | The majority of the coding region was replaced with a neomycin selection cassette. RT-PCR analysis showed an absence of transcript in homozygous mutant mice. [MGI Ref ID J:40377] | ||
| Allele Symbol | Ins2tm1Jja | ||
| Allele Name | targeted mutation 1, Jacques Jami | ||
| Common Name(s) | Ins2-; proins-2-; proinsulin 2-; | ||
| Mutation Made By | Jacques Jami, INSERM | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Ins2, insulin II | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AA986540; Ins-2; InsII; Mody; Mody4; expressed sequence AA986540; maturity onset diabetes of the young; maturity onset diabetes of the young 4; | ||
| Molecular Note | The majority of the coding region was replaced by the insertion of a lacZ-neo fusion gene. The expression of lacZ was found to be under the control of the endogenous promoter via cytochemical assays. [MGI Ref ID J:40377] | ||
| Allele Symbol | Tg(Ins2*Y16A)1Ell | ||
| Allele Name | transgene insertion 1, John F Elliot | ||
| Common Name(s) | tg B:16 alanine, line B; | ||
| Mutation Made By | John Elliott, University of Alberta | ||
| Strain of Origin | NOD/MrkTac | ||
| Expressed Gene | Ins2, insulin 2, rat | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | This transgenic construct contains the rat insulin7 construct of the rat insulin 2 gene fused to a mutated preproinsulin II cDNA where the tyrosine at position 16 of the B chain was replaced with alanine. The transgene is expressed in pancreatic beta cells. [MGI Ref ID J:97755] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Ins1tm1Jja
Ins2tm1Jja
Tg(Ins2*Y16A)
Related Strains
Strains carrying Ins1tm1Jja allele
005035 NOD.129S2(B6)-Ins1tm1Jja/GseJ 005525 NOD.Cg-Tg(Ins2*Y16A)3Ell Ins1tm1Jja Ins2tm1Jja/GseJ View Strains carrying Ins1tm1Jja (2 strains)
005036 NOD.129S2(B6)-Ins2tm1Jja/GseJ 005525 NOD.Cg-Tg(Ins2*Y16A)3Ell Ins1tm1Jja Ins2tm1Jja/GseJ
005522 NOD-Tg(Ins2*Y16A)1Ell/GseJ
006872 B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J 006864 B6.Cg-Tg(Ins1-EGFP)1Hara/J 008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Ins1 relatedDiabetes and Obesity Research
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Autoimmunity (autoimmune pancreatitis and sialoadenitis)
Research Tools
Diabetes and Obesity Research
Ins2 relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Ins1tm1Jja relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Ins2tm1Jja relatedDiabetes and Obesity Research
Impaired Insulin Processing
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM)
Impaired Insulin Processing
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM)
References
Selected Reference(s)
Additional ReferencesNakayama M; Moriyama H; Abiru N; Babu SR; Sikora K; Li M; Miao D; Hutton JC; Elliott JF; Eisenbarth GS. 2004. Establishment of native insulin-negative NOD mice and the methodology to distinguish specific insulin knockout genotypes and a B:16 alanine preproinsulin transgene. Ann N Y Acad Sci 1037:193-8. [PubMed: 15699516] [MGI Ref ID J:97755]
Price and Supply Information
| Strain Name: | NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ |
| Stock Number: | 005524 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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