Strain Name:

NOD.Cg-Tg(Ins2*Y16A)3Ell Ins1tm1Jja Ins2tm1Jja/GseJ

Stock Number:

005525

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Purchasing Information

Description

Strain Information

Type Coisogenic; Congenic; Mutant Strain; Targeted Mutation; Transgenic;
Specieslaboratory mouse
Background Strain NOD/MrkTac
Donor Strain D3 (129S2/SvPas)
H2 Haplotypeg7
GenerationN10F9+F3p
 
Donating Investigator George Eisenbarth,   U of Colorado

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Description
Expression has been reported in the pancreatic islets and thymus of NOD mice carrying the Tg(Ins2*Y16A)3Ell mutation. Approximately 15% of line F female transgenic mice become diabetic in the presence of native insulin genes by 35 weeks of age. NOD female transgenic mice lacking both Ins1 and Ins2 fail to produce insulin autoantibodies, and there is no diabetes or insulitis at 26 weeks of age, but sialitis is present. In contrast, transgenic mice in the presence of Ins1 and lacking Ins2 develop diabetes in 75% of the animals by 25 weeks of age (Nakayama et al, 2004, 2005).

This stock is useful to study insulin-reactive autoimmunity.

Development
NOD-Tg(Ins2*Y16A)3Ell/GseJ expresses the rat insulin 7 promoter fused to a mutated preproinsulin II cDNA, where the tyrosine at position 16 of the b chain was replaced with alanine. This transgene was inserted by Nakayama et al, 2004 directly into NOD/MrkTac oocytes. Offspring from the founder line F was further crossed to NOD.Ins1-/- and NOD.Ins2-/- prior to intercrossing for homozygosity of the Ins1 and Ins2 targeted mutations. In 2006, the T1DR received NOD.Cg-Tg(Ins2*Y16A)3Ell Ins1tm1Jja Ins2tm1Jja/GseJ at generation N10F9.

Control Information

  Control
   005035 NOD.129S2(B6)-Ins1tm1Jja/GseJ
   005036 NOD.129S2(B6)-Ins2tm1Jja/GseJ
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ins1tm1Jja allele
005035   NOD.129S2(B6)-Ins1tm1Jja/GseJ
005524   NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ
View Strains carrying   Ins1tm1Jja     (2 strains)

Strains carrying   Ins2tm1Jja allele
005036   NOD.129S2(B6)-Ins2tm1Jja/GseJ
005524   NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ
View Strains carrying   Ins2tm1Jja     (2 strains)

Strains carrying   Tg(Ins2*Y16A)3Ell allele
005523   NOD-Tg(Ins2*Y16A)3Ell/GseJ
View Strains carrying   Tg(Ins2*Y16A)3Ell     (1 strain)

View Strains carrying other alleles of Ins1     (5 strains)

Strains carrying other alleles of Ins2
005534   B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ
006860   B6.129-Ins2Akita Bdkrb2tm1Jfh/SmiJ
005500   B6.C-Tg(Ins2-GP)34-20Olds/MvhJ
005715   B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ
006580   B6.Cg-Ins2Akita Ldlrtm1Her/J
004369   B6.Cg-Rag1tm1Mom Ins2Akita/J
004826   B6.Cg-Tg(Ins2-NP)25-3Olds/MhvJ
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
005713   C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ
005533   C.Cg-Tg(Ins2-HA)165Bri/ShrmJ
004827   C.Cg-Tg(Ins2-NP)25-3Olds/MvhJ
003548   C57BL/6-Ins2Akita/J
005432   C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ
005433   C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
005431   C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
007562   D2.B6-Ins2Akita/MatbJ
005564   FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
006867   FVB.B6-Ins2Akita/MlnJ
008232   FVB/N-Tg(Ins2-IAPP)RHFSoel/J
005739   NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ
005522   NOD-Tg(Ins2*Y16A)1Ell/GseJ
003499   NOD-Tg(Ins2-Fasl)24Ach
007840   NOD.Cg-Prkdcscid Tg(Ins2-CD86)12B70Flv/FswJ
004346   NOD.Cg-Prkdcscid Tg(Ins2-CD80)3B7Flv/DvsJ
004230   NOD.Cg-Prkdcscid Tg(Ins2-E3)1Dvs/DvsJ
003843   NOD.Cg-Prkdcscid Tg(Ins2-GAD2)1Lt/LtJ
003844   NOD.Cg-Prkdcscid Tg(Ins2-GAD2)2Lt/LtJ
005524   NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ
006254   NOD.Cg-Tg(Ins2-Ccl21b)2Cys/JbsJ
006154   NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ
003869   NOD.Cg-Tg(Ins2-E3)1Dvs/DvsJ
005685   NOD.Cg-Tg(Ins2-HA)165Bri/ShrmJ
002380   NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
005870   NOD/ShiLt(Cg)-Tg(Ins2-GAD2)2Lt/J
006777   NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ
005733   NOD/ShiLt-Tg(Ins2-Fas*I246N)1Ach/AchJ
003074   NOD/ShiLt-Tg(Ins2-GAD2)1Lt/LtJ
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
002033   NOD/ShiLt-Tg(RipTAg)1Lt/J
004968   NOD/ShiLtDvs-Tg(Ins2-E3*734)3Dvs/DvsJ
004990   NOD/ShiLtDvs-Tg(Ins2-E3*734)4Dvs/DvsJ
005714   NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
View Strains carrying other alleles of Ins2     (49 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Related Disease (OMIM) Terms

NOTDiabetes Mellitus, Insulin-Dependent; IDDM

Mammalian Phenotype Terms assigned by genotype

Ins1tm1Jja/Ins1tm1Jja Ins2tm1Jja/Ins2tm1Jja Tg(Ins2*Y16A)3Ell/0

        NOD.Cg-Ins1tm1Jja Ins2tm1Jja Tg(Ins2*Y16A)3Ell
  • immune system phenotype
  • decreased anti-insulin autoantibody level (MGI Ref ID J:98583)
    • females lacking both insulin genes do not produce insulin autoantibodies
  • decreased susceptibility to autoimmune diabetes (MGI Ref ID J:98583)
    • no female double knockout mice carrying the transgene on a NOD background develop diabetes over 30 weeks of observation
  • salivary gland inflammation (MGI Ref ID J:98583)
    • at 26 weeks of age, sialitis is observed but not insulitis
  • digestive/alimentary phenotype
  • salivary gland inflammation (MGI Ref ID J:98583)
    • at 26 weeks of age, sialitis is observed but not insulitis
  • endocrine/exocrine gland phenotype
  • salivary gland inflammation (MGI Ref ID J:98583)
    • at 26 weeks of age, sialitis is observed but not insulitis

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Autoimmunity (autoimmune pancreatitis and sialoadenitis)

Research Tools
Diabetes and Obesity Research

Ins1 related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

Ins2 related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

Ins1tm1Jja related

Diabetes and Obesity Research
Impaired Insulin Processing
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM)

Ins2tm1Jja related
Impaired Insulin Processing
Insulin Receptors and Growth Factors
Type 1 Diabetes (IDDM)

Genes & Alleles

Gene & Allele Information

Allele Symbol Ins1tm1Jja
Allele Name targeted mutation 1, Jacques Jami
Common Name(s) Ins1-;
Mutation Made By Jacques Jami,   INSERM
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Ins1, insulin I
Chromosome 19
Gene Common Name(s) Ins-1; Ins2-rs1; insulin 2, related sequence 1; insulin I or insulin pseudogene;
Molecular Note The majority of the coding region was replaced with a neomycin selection cassette. RT-PCR analysis showed an absence of transcript in homozygous mutant mice. [MGI Ref ID J:40377]
 
Allele Symbol Ins2tm1Jja
Allele Name targeted mutation 1, Jacques Jami
Common Name(s) Ins2-; proins-2-; proinsulin 2-;
Mutation Made By Jacques Jami,   INSERM
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Ins2, insulin II
Chromosome 7
Gene Common Name(s) AA986540; ILPR; IRDN; Ins-2; InsII; Mody; Mody4; expressed sequence AA986540; maturity onset diabetes of the young; maturity onset diabetes of the young 4;
Molecular Note The majority of the coding region was replaced by the insertion of a lacZ-neo fusion gene. The expression of lacZ was found to be under the control of the endogenous promoter via cytochemical assays. [MGI Ref ID J:40377]
 
Allele Symbol Tg(Ins2*Y16A)3Ell
Allele Name transgene insertion 3, John F Elliot
Common Name(s) tg B:16 alanine, line F;
Mutation Made By John Elliott,   University of Alberta
Strain of OriginNOD/MrkTac
Expressed Gene Ins2, insulin 2, rat
Promoter Ins2, insulin 2, rat
Molecular Note This transgenic construct contains the rat insulin7 construct of the rat insulin 2 gene fused to a mutated preproinsulin II cDNA where the tyrosine at position 16 of the B chain was replaced with alanine. The transgene is expressed in pancreatic beta cells. [MGI Ref ID J:97755]

Genotyping

Genotyping Information

Genotyping Protocols

Ins1tm1Jja, SEP PCR, vers. 1
Ins2tm1Jja, STD PCR, vers. 1
Tg(Ins2*Y16A), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Hediger MA; Budarf ML; Emanuel BS; Mohandas TK; Wright EM. 1989. Assignment of the human intestinal Na+/glucose cotransporter gene (SGLT1) to the q11.2----qter region of chromosome 22. Genomics 4(3):297-300. [PubMed: 2714793]  [MGI Ref ID J:9755]

Additional References

Ins1tm1Jja related

Babaya N; Nakayama M; Moriyama H; Gianani R; Still T; Miao D; Yu L; Hutton JC; Eisenbarth GS. 2006. A new model of insulin-deficient diabetes: male NOD mice with a single copy of Ins1 and no Ins2. Diabetologia 49(6):1222-8. [PubMed: 16612590]  [MGI Ref ID J:111475]

Duvillie B; Cordonnier N; Deltour L; Dandoy-Dron F; Itier JM ; Monthioux E ; Jami J ; Joshi RL ; Bucchini D. 1997. Phenotypic alterations in insulin-deficient mutant mice. Proc Natl Acad Sci U S A 94(10):5137-40. [PubMed: 9144203]  [MGI Ref ID J:40377]

Duvillie B; Currie C; Chrones T; Bucchini D; Jami J; Joshi RL; Hill DJ. 2002. Increased islet cell proliferation, decreased apoptosis, and greater vascularization leading to beta-cell hyperplasia in mutant mice lacking insulin. Endocrinology 143(4):1530-7. [PubMed: 11897712]  [MGI Ref ID J:106827]

Leroux L; Desbois P; Lamotte L; Duvillie B; Cordonnier N; Jackerott M; Jami J; Bucchini D; Joshi RL. 2001. Compensatory responses in mice carrying a null mutation for Ins1 or Ins2. Diabetes 50 Suppl 1:S150-3. [PubMed: 11272179]  [MGI Ref ID J:77595]

Moriyama H; Abiru N; Paronen J; Sikora K; Liu E; Miao D; Devendra D; Beilke J; Gianani R; Gill RG; Eisenbarth GS. 2003. Evidence for a primary islet autoantigen (preproinsulin 1) for insulitis and diabetes in the nonobese diabetic mouse. Proc Natl Acad Sci U S A 100(18):10376-81. [PubMed: 12925730]  [MGI Ref ID J:85309]

Moriyama H; Nagata M; Arai T; Okumachi Y; Yamada K; Kotani R; Yasuda H; Hara K; Yokono K. 2007. Insulin as a T cell antigen in type 1 diabetes supported by the evidence from the insulin knockout NOD mice. Diabetes Res Clin Pract 77 Suppl 1:S155-60. [PubMed: 17459508]  [MGI Ref ID J:136741]

Nakayama M; Abiru N; Moriyama H; Babaya N; Liu E; Miao D; Yu L; Wegmann DR; Hutton JC; Elliott JF; Eisenbarth GS. 2005. Prime role for an insulin epitope in the development of type 1 diabetes in NOD mice. Nature 435(7039):220-3. [PubMed: 15889095]  [MGI Ref ID J:98583]

Nakayama M; Babaya N; Miao D; Sikora K; Elliott JF; Eisenbarth GS. 2005. Thymic expression of mutated B16:A preproinsulin messenger RNA does not reverse acceleration of NOD diabetes associated with insulin 2 (thymic expressed insulin) knockout. J Autoimmun 25(3):193-8. [PubMed: 16289958]  [MGI Ref ID J:106579]

Nakayama M; Beilke JN; Jasinski JM; Kobayashi M; Miao D; Li M; Coulombe MG; Liu E; Elliott JF; Gill RG; Eisenbarth GS. 2007. Priming and effector dependence on insulin B:9-23 peptide in NOD islet autoimmunity. J Clin Invest 117(7):1835-43. [PubMed: 17607359]  [MGI Ref ID J:124210]

Schechter R; Beju D; Miller KE. 2005. The effect of insulin deficiency on tau and neurofilament in the insulin knockout mouse. Biochem Biophys Res Commun 334(4):979-86. [PubMed: 16039605]  [MGI Ref ID J:99957]

Ins2tm1Jja related
Babaya N; Nakayama M; Moriyama H; Gianani R; Still T; Miao D; Yu L; Hutton JC; Eisenbarth GS. 2006. A new model of insulin-deficient diabetes: male NOD mice with a single copy of Ins1 and no Ins2. Diabetologia 49(6):1222-8. [PubMed: 16612590]  [MGI Ref ID J:111475]

Dubois-Lafforgue D; Mogenet L; Thebault K; Jami J; Krief P; Boitard C. 2002. Proinsulin 2 knockout NOD mice: a model for genetic variation of insulin gene expression in type 1 diabetes. Diabetes 51 Suppl 3:S489-93. [PubMed: 12475795]  [MGI Ref ID J:107164]

Duvillie B; Bucchini D; Tang T; Jami J; Paldi A. 1998. Imprinting at the mouse Ins2 locus: evidence for cis- and trans-allelic interactions. Genomics 47(1):52-7. [PubMed: 9465295]  [MGI Ref ID J:45695]

Duvillie B; Cordonnier N; Deltour L; Dandoy-Dron F; Itier JM ; Monthioux E ; Jami J ; Joshi RL ; Bucchini D. 1997. Phenotypic alterations in insulin-deficient mutant mice. Proc Natl Acad Sci U S A 94(10):5137-40. [PubMed: 9144203]  [MGI Ref ID J:40377]

Duvillie B; Currie C; Chrones T; Bucchini D; Jami J; Joshi RL; Hill DJ. 2002. Increased islet cell proliferation, decreased apoptosis, and greater vascularization leading to beta-cell hyperplasia in mutant mice lacking insulin. Endocrinology 143(4):1530-7. [PubMed: 11897712]  [MGI Ref ID J:106827]

Faideau B; Briand JP; Lotton C; Tardivel I; Halbout P; Jami J; Elliott JF; Krief P; Muller S; Boitard C; Carel JC. 2004. Expression of preproinsulin-2 gene shapes the immune response to preproinsulin in normal mice. J Immunol 172(1):25-33. [PubMed: 14688305]  [MGI Ref ID J:87572]

Faideau B; Lotton C; Lucas B; Tardivel I; Elliott JF; Boitard C; Carel JC. 2006. Tolerance to proinsulin-2 is due to radioresistant thymic cells. J Immunol 177(1):53-60. [PubMed: 16785498]  [MGI Ref ID J:134383]

Jasinski JM; Yu L; Nakayama M; Li MM; Lipes MA; Eisenbarth GS; Liu E. 2006. Transgenic insulin (B:9-23) T-cell receptor mice develop autoimmune diabetes dependent upon RAG genotype, H-2g7 homozygosity, and insulin 2 gene knockout. Diabetes 55(7):1978-84. [PubMed: 16804066]  [MGI Ref ID J:111874]

Lamotte L; Jackerott M; Bucchini D; Jami J; Joshi RL; Deltour L. 2004. Knock-in of diphteria toxin A chain gene at Ins2 locus: effects on islet development and localization of Ins2 expression in the brain. Transgenic Res 13(5):463-73. [PubMed: 15587270]  [MGI Ref ID J:94589]

Leroux L; Desbois P; Lamotte L; Duvillie B; Cordonnier N; Jackerott M; Jami J; Bucchini D; Joshi RL. 2001. Compensatory responses in mice carrying a null mutation for Ins1 or Ins2. Diabetes 50 Suppl 1:S150-3. [PubMed: 11272179]  [MGI Ref ID J:77595]

Moriyama H; Abiru N; Paronen J; Sikora K; Liu E; Miao D; Devendra D; Beilke J; Gianani R; Gill RG; Eisenbarth GS. 2003. Evidence for a primary islet autoantigen (preproinsulin 1) for insulitis and diabetes in the nonobese diabetic mouse. Proc Natl Acad Sci U S A 100(18):10376-81. [PubMed: 12925730]  [MGI Ref ID J:85309]

Moriyama H; Nagata M; Arai T; Okumachi Y; Yamada K; Kotani R; Yasuda H; Hara K; Yokono K. 2007. Insulin as a T cell antigen in type 1 diabetes supported by the evidence from the insulin knockout NOD mice. Diabetes Res Clin Pract 77 Suppl 1:S155-60. [PubMed: 17459508]  [MGI Ref ID J:136741]

Nakayama M; Abiru N; Moriyama H; Babaya N; Liu E; Miao D; Yu L; Wegmann DR; Hutton JC; Elliott JF; Eisenbarth GS. 2005. Prime role for an insulin epitope in the development of type 1 diabetes in NOD mice. Nature 435(7039):220-3. [PubMed: 15889095]  [MGI Ref ID J:98583]

Nakayama M; Babaya N; Miao D; Sikora K; Elliott JF; Eisenbarth GS. 2005. Thymic expression of mutated B16:A preproinsulin messenger RNA does not reverse acceleration of NOD diabetes associated with insulin 2 (thymic expressed insulin) knockout. J Autoimmun 25(3):193-8. [PubMed: 16289958]  [MGI Ref ID J:106579]

Nakayama M; Beilke JN; Jasinski JM; Kobayashi M; Miao D; Li M; Coulombe MG; Liu E; Elliott JF; Gill RG; Eisenbarth GS. 2007. Priming and effector dependence on insulin B:9-23 peptide in NOD islet autoimmunity. J Clin Invest 117(7):1835-43. [PubMed: 17607359]  [MGI Ref ID J:124210]

Schechter R; Beju D; Miller KE. 2005. The effect of insulin deficiency on tau and neurofilament in the insulin knockout mouse. Biochem Biophys Res Commun 334(4):979-86. [PubMed: 16039605]  [MGI Ref ID J:99957]

Thebault-Baumont K; Dubois-Laforgue D; Krief P; Briand JP; Halbout P; Vallon-Geoffroy K; Morin J; Laloux V; Lehuen A; Carel JC; Jami J; Muller S; Boitard C. 2003. Acceleration of type 1 diabetes mellitus in proinsulin 2-deficient NOD mice. J Clin Invest 111(6):851-7. [PubMed: 12639991]  [MGI Ref ID J:82522]

Tg(Ins2*Y16A)3Ell related
Nakayama M; Abiru N; Moriyama H; Babaya N; Liu E; Miao D; Yu L; Wegmann DR; Hutton JC; Elliott JF; Eisenbarth GS. 2005. Prime role for an insulin epitope in the development of type 1 diabetes in NOD mice. Nature 435(7039):220-3. [PubMed: 15889095]  [MGI Ref ID J:98583]

Nakayama M; Babaya N; Miao D; Sikora K; Elliott JF; Eisenbarth GS. 2005. Thymic expression of mutated B16:A preproinsulin messenger RNA does not reverse acceleration of NOD diabetes associated with insulin 2 (thymic expressed insulin) knockout. J Autoimmun 25(3):193-8. [PubMed: 16289958]  [MGI Ref ID J:106579]

Nakayama M; Beilke JN; Jasinski JM; Kobayashi M; Miao D; Li M; Coulombe MG; Liu E; Elliott JF; Gill RG; Eisenbarth GS. 2007. Priming and effector dependence on insulin B:9-23 peptide in NOD islet autoimmunity. J Clin Invest 117(7):1835-43. [PubMed: 17607359]  [MGI Ref ID J:124210]

Nakayama M; Moriyama H; Abiru N; Babu SR; Sikora K; Li M; Miao D; Hutton JC; Elliott JF; Eisenbarth GS. 2004. Establishment of native insulin-negative NOD mice and the methodology to distinguish specific insulin knockout genotypes and a B:16 alanine preproinsulin transgene. Ann N Y Acad Sci 1037:193-8. [PubMed: 15699516]  [MGI Ref ID J:97755]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryGenotypes available: Tg/?, Ins1-/-, Ins2-/-
Breeders available: Tg/?, Ins1-/-, Ins2-/- female x Tg/?, Ins1-/-, Ins2-/- male
Affected mutant: Tg/?, Ins1-/-, Ins2-/-
TJL mating scheme: Tg/?, Ins1-/-, Ins2-/- female x Tg/?, Ins1-/-, Ins2-/- male

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Type 1 Diabetes Repository collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Type 1 Diabetes Repository collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   005035 NOD.129S2(B6)-Ins1tm1Jja/GseJ
   005036 NOD.129S2(B6)-Ins2tm1Jja/GseJ
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form


(3.1.1)