| |||||||||
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation ?+N1F9 (04-DEC-07) Donating Investigator David Ron, NYU School of Medicine Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (MRNA or protein) is detected by Northern or Western blot analysis of toxin challenged mouse embryonic fibroblasts (MEFs). MEFs and renal proximal tubular epithelial cells have decreased apoptosis in response to endoplasmic reticulum stress induced by the toxin, tunicamycin. Pancreatic islets cells are more resistant to nitric oxide induced apoptosis. MEFs exhibit a delayed onset of unfolded protein response (UPR) target gene expression when treated with tunicamycin. This mutant mouse strain may be useful in studies of apoptosis and pathogenesis due to endoplasmic reticulum stress.Development
A floxed PGK-Neo cassette was used to disrupt almost all the coding region in exons 3 and 4. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The floxed selection cassette was later removed by Cre recombinase excision by crossing the mice to a Cre deleter strain. The animals were then crossed to C57BL/6 mice for 5 generations.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
Genetic Quality Control Annual Report
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ddit3tm1Dron/Ddit3tm1Dron
either: (involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)
- cellular phenotype
- decreased apoptosis (MGI Ref ID J:52729)
- MEFs prepared from E13.5 embryos exhibit less programmed cell death when challenged with agents that perturb ER function
- homozygotes treated with tunicamycin to induce ER stress and renal insufficiency exhibit decreased programmed cell death in the renal proximal tubular epithelium
- homeostasis/metabolism phenotype
- abnormal response to injury (MGI Ref ID J:52729)
- homozygotes treated with tunicamycin to induce ER stress and renal insufficiency exhibit decreased programmed cell death in the renal proximal tubular epithelium compared to controls despite impaired renal function
Ddit3tm1Dron/Ddit3tm1Dron
involves: 129S1/Sv * 129X1/SvJ * FVB/N
- immune system phenotype
- decreased susceptibility to viral infection (MGI Ref ID J:112354)
- homozygotes infected with a neurovirulent virus, Moloney MLV-ts1, exhibit a small, but significant, delay of about 4.5 days in the incubation period of the disease that correlates with a decrease in early virus replication
Ddit3tm1Dron/Ddit3tm1Dron
involves: 129S1/Sv * 129X1/SvJ
- endocrine/exocrine gland phenotype
- *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:129974)
- homozygotes have a normal pancreas
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Apoptosis Research
Endogenous Regulators
Extracellular Modulators
Cell Biology Research
DNA Damage Response
Research Tools
Apoptosis Research
Toxicology Research (free radical research)
| Allele Symbol | Ddit3tm1Dron | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, David Ron | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | chop-; | ||
| Mutation Made By | Rivka Jungreis, | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Ddit3, DNA-damage inducible transcript 3 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | C/EBP homoologous protein 10; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; MGC124604; MGC4154; | ||
| Molecular Note | A PGK-neomycin resistance cassette replaced the coding region in parts of exons 3 and 4. Northern blot analyses did not detect transcript in MEFs from homozygous mutant E13.5 embryos. [MGI Ref ID J:52729] | ||
Genotyping Protocols
Ddit3tm1Dron, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Marciniak SJ; Yun CY; Oyadomari S; Novoa I; Zhang Y; Jungreis R; Nagata K; Harding HP; Ron D. 2004. CHOP induces death by promoting protein synthesis and oxidation in the stressed endoplasmic reticulum. Genes Dev 18(24):3066-77. [PubMed: 15601821] [MGI Ref ID J:95320]
Ddit3tm1Dron relatedClase AC; Dimcheff DE; Favara C; Dorward D; McAtee FJ; Parrie LE; Ron D; Portis JL. 2006. Oligodendrocytes are a major target of the toxicity of spongiogenic murine retroviruses. Am J Pathol 169(3):1026-38. [PubMed: 16936275] [MGI Ref ID J:112354]
Iida K; Li Y; McGrath BC; Frank A; Cavener DR. 2007. PERK eIF2 alpha kinase is required to regulate the viability of the exocrine pancreas in mice. BMC Cell Biol 8:38. [PubMed: 17727724] [MGI Ref ID J:129974]
Pennuto M; Tinelli E; Malaguti M; Del Carro U; D'Antonio M; Ron D; Quattrini A; Feltri ML; Wrabetz L. 2008. Ablation of the UPR-mediator CHOP restores motor function and reduces demyelination in Charcot-Marie-Tooth 1B mice. Neuron 57(3):393-405. [PubMed: 18255032] [MGI Ref ID J:135317]
Seimon TA; Obstfeld A; Moore KJ; Golenbock DT; Tabas I. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proc Natl Acad Sci U S A 103(52):19794-9. [PubMed: 17167049] [MGI Ref ID J:118240]
Zinszner H; Kuroda M; Wang X; Batchvarova N; Lightfoot RT; Remotti H; Stevens JL; Ron D. 1998. CHOP is implicated in programmed cell death in response to impaired function of the endoplasmic reticulum. Genes Dev 12(7):982-95. [PubMed: 9531536] [MGI Ref ID J:52729]
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Homozygous for Ddit3tm1Dron *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.20 Homozygous for Ddit3tm1Dron x Homozygous for Ddit3tm1Dron
| Supply Notes |
|
|---|
| Pricing for International shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Homozygous for Ddit3tm1Dron *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.60 Homozygous for Ddit3tm1Dron x Homozygous for Ddit3tm1Dron
| Supply Notes |
|
|---|
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.