Strain Name: |
B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ |
|---|---|
Stock Number: |
005534 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B10.Cg Hc1 H2d H2-T18c-Tg(Ins2-HA)165Bri/ShrmJ (Changed: 18-JAN-06
) |
| Genes & Alleles | H2; H2d; Ins2; HA; Tg(Ins2-HA)165Bri; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain B10.D2-Hc1 H2d H2-T18c/nSn Donor Strain (C57BL/6 x SJL)F2 Donating Investigator Linda Sherman, The Scripps Research Institute H2 Haplotype d Appearance
black
Related Genotype: A/? Tyrc/TyrcStrain Description
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. Mice homozygote for the transgene have silver grey fur color. Hemizygous and wildtype mice are black. Immunohistochemistry reveals pancreatic islet cell expression of the transgene and no expression in the spleen, kidney or thymus. Isolated islets stain normally for insulin and are morphologically indistinguishable from control islets. Additional functional studies found no expression in bone marrow. Histology revealed no insulitis and the single transgenic mice do not become diabetic. T-cell proliferation assays, Cytotoxic Lymphocyte (CTL) assays, and adoptive transfer studies performed using transgenic mice indicate significantly reduced class 1 and class II T-cell responses compared to controls. Hemagglutination inhibition assays of sera from HA primed transgenic mice indicate antibody titers slightly lower but nearly equivalent to HA primed control mice. Antibody titers of all transgenic mice tested were significantly higher than preimmune levels. When mated with Tg(TcraCl4,TcrbCl4) mice, the double transgenic neonates become spontaneously diabetic after birth and die within 10 days.This is a good model for studying peripheral tolerance.
Strain Development
B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ expresses influenza hemagglutinin (HA) molecule from the well characterized influenza A/PR/8/34 under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into (C57BL/6J x SJL)F2 oocytes. Resulting progeny from founder line 2917-5 was backcrossed to H-2d stains BALB/cBy for 10 generations and B10.D2-Hc1 H2d H2-T18c/nSnJ) for 10 generations prior to intercrossing. In 2005, t he Jackson Laboratory received B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ at generation N10F20.
Gene & Allele Details
| Allele Symbol | H2d | ||
|---|---|---|---|
| Allele Name | d variant | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB | ||
| Allele Symbol | Tg(Ins2-HA)165Bri | ||
| Allele Name | transgene insertion 165, Ralph Brinster | ||
| Common Name(s) | 2917-5; Ins-HA; InsHA; Tg(Ins-2,HA)Bri165; | ||
| Mutation Made By | Ralph Brinster, University of Pennsylvania | ||
| Expressed Gene | HA, influenza hemagglutinin, | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | The transgenic construct consisted of the rat insulin 2 promoter fused to sequence encoding influenza hemagglutinin (HA) followed by the 3' untranslated region and polyadenylation signal from the H2-Ea. HA was from influenza A/PR/8/34. This rat insulin 2 promoter is active in pancreatic beta-cells. [MGI Ref ID J:86430] | ||
Control Information
| Control | ||
|---|---|---|
| 000463 B10.D2-Hc1 H2d H2-T18c/nSnJ | (approximate) | |
| Stock number 000463 - B10.D2-Hc1 H2d H2-T18c/nSnJ serves as an approximate control. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-HA)165Bri
Related Strains
Strains carrying H2d allele
005308 B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 005895 B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J 000462 B10.D2-H2d/n2SnJ 000460 B10.D2-Hc0 H2d H2-T18c/o2SnJ 000461 B10.D2-Hc0 H2d H2-T18c/oSnJ 000463 B10.D2-Hc1 H2d H2-T18c/nSnJ 003147 B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J 000360 B6.C-H2d Mdmg1BALB/cBy/aByJ 000359 B6.C-H2d/bByJ 001893 BRVR.D2-H2d/J 000437 D1.C-H2d H2-T18c/SnJ 003153 WLC.C-H2d.GR-Mtv2/MorJ 003154 WLC.C-H2d/MorJ View Strains carrying H2d (13 strains)
005533 C.Cg-Tg(Ins2-HA)165Bri/ShrmJ 005685 NOD.Cg-Tg(Ins2-HA)165Bri/ShrmJ
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
H2d relatedImmunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation
Rearranged Antigen-Specific T Cell Receptor Transgenes
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Cancer Research (tumor immunology)
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
References
Selected Reference(s)
Additional ReferencesLo D; Freedman J; Hesse S; Palmiter RD; Brinster RL; Sherman LA. 1992. Peripheral tolerance to an islet cell-specific hemagglutinin transgene affects both CD4+ and CD8+ T cells. Eur J Immunol 22(4):1013-22. [PubMed: 1348026] [MGI Ref ID J:86430]
Price and Supply Information
| Strain Name: | B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ |
| Stock Number: | 005534 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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