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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x F1 (Female x Male) 01-MAR-06 Species laboratory mouse Generation N?+N12 (23-JAN-12)
Generation DefinitionsDonating Investigator Jonathan Epstein, University of Pennsylvania Description
This strain expresses Cre recombinase from the endogenous Pax3 locus. Expression of the targeted gene product (mRNA and protein) mimics endogenous gene expression as detected by in situ hybridization and immunohistochemistry of homozygous embryos aged E12.5. No endogenous Pax3 gene product (protein) is detected in homozygotes and approximately one half of the endogenous gene product (protein) is detected in heterozygotes by Western blot analysis. Cre recombinase expression is detected in the dorsal neural tube and somites of E9 to 11.5 embryos and in the cardiac neural crest cells and colonic epithelia of E11.5 embryos. Recombination occurs in neural crest and somite derivatives of later gestation embryos. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. At age E13.5 homozygous embryos display severe cardiac and neural tube defects (exencephaly), absent limb musculature and reduced or absent dorsal root ganglia. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Half of the heterozygous mice exhibit a white belly spot. The phenotype exhibited by this mutant strain is similar to the phenotype observed in mice carrying the spontaneous splotch mutant allele of Pax3. This mutant mouse strain represents a model that may be useful in studies of development and lineage mapping of neural crest and somite derivatives.Development
A targeting vector containing Cre coding sequence, a loxP site flanked PGK-neo cassette and a PGK-herpes simplex virus thymidine kinase gene was used to disrupt most of exon 1 including the initiation codon and 69 bp of the 5' flanking region. The endogenous Pax3 promoter drives expression of the Cre recombinase through the in-frame insertion of the Cre coding sequence to the first exon of the Pax3 gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice once and are maintained on a mixed B6;129 background.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Pax3
000311 B6-Pax3Sp.Cg-N/J 000565 C57BL/6J-Pax3Sp-d/J 002469 C57BL/6J-Pax3Sp/J 002902 STOCK Pax3Sp Mlphln/J View Strains carrying other alleles of Pax3 (4 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Pax3tm1(cre)Joe/Pax3tm1(cre)Joe
Background Not Specified
- mortality/aging
- complete postnatal lethality
- despite normal numbers at E17.5, no mice survive beyond P2 (MGI Ref ID J:137730)
- nervous system phenotype
- abnormal neural tube morphology/development
- at E13.5, mice exhibit neural tube defects either at the lumbar or cranial level or both (MGI Ref ID J:137730)
- open neural tube (MGI Ref ID J:137730)
- exencephaly (MGI Ref ID J:137730)
- muscle phenotype
- abnormal diaphragm morphology
- at E17.5, the diaphragm is thin and lacks muscle (MGI Ref ID J:137730)
- limbs/digits/tail phenotype
- abnormal hindlimb morphology (MGI Ref ID J:137730)
- embryogenesis phenotype
- abnormal neural tube morphology/development
- at E13.5, mice exhibit neural tube defects either at the lumbar or cranial level or both (MGI Ref ID J:137730)
- open neural tube (MGI Ref ID J:137730)
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
multiple
Limb Patterning Defects
Neural Tube Defects
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Neural Tube Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Pax3tm1(cre)Joe | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jonathan A Epstein | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Pax3-Cre; Pax3Cre; Pax3Cre-KI; | ||
| Mutation Made By | Kurt Engleka, University of Pennsylvania | ||
| Site of Expression | dorsal neural tube and somites of embryonic day 9-11.5 embryos and in the cardiac neural crest cells and colonic epithelia of embryonic day 11.5 embryos | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Pax3 | ||
| Molecular Note | A targeting vector was desinged to insert the cre recombinase cDNA followed by a stop codon and a polyA signal in place of exon 1 of the endogenous locus. [MGI Ref ID J:96431] | ||
| Gene Symbol and Name | Pax3, paired box gene 3 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CDHS; HUP2; Pax-3; Sp; WS1; WS3; splotch; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Pax3tm1(cre)Joe, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lang D; Lu MM; Huang L; Engleka KA; Zhang M; Chu EY; Lipner S; Skoultchi A; Millar SE; Epstein JA. 2005. Pax3 functions at a nodal point in melanocyte stem cell differentiation. Nature 433(7028):884-7. [PubMed: 15729346] [MGI Ref ID J:96431]
Additional References
Pax3tm1(cre)Joe relatedDegenhardt KR; Milewski RC; Padmanabhan A; Miller M; Singh MK; Lang D; Engleka KA; Wu M; Li J; Zhou D; Antonucci N; Li L; Epstein JA. 2010. Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expressions. Dev Biol 339(2):519-27. [PubMed: 20045680] [MGI Ref ID J:159124]
Engleka KA; Gitler AD; Zhang M; Zhou DD; High FA; Epstein JA. 2005. Insertion of Cre into the Pax3 locus creates a new allele of Splotch and identifies unexpected Pax3 derivatives. Dev Biol 280(2):396-406. [PubMed: 15882581] [MGI Ref ID J:98272]
Engleka KA; Wu M; Zhang M; Antonucci NB; Epstein JA. 2007. Menin is required in cranial neural crest for palatogenesis and perinatal viability. Dev Biol 311(2):524-37. [PubMed: 17927973] [MGI Ref ID J:127545]
Freyer L; Aggarwal V; Morrow BE. 2011. Dual embryonic origin of the mammalian otic vesicle forming the inner ear. Development 138(24):5403-14. [PubMed: 22110056] [MGI Ref ID J:178934]
Goljanek-Whysall K; Sweetman D; Abu-Elmagd M; Chapnik E; Dalmay T; Hornstein E; Munsterberg A. 2011. MicroRNA regulation of the paired-box transcription factor Pax3 confers robustness to developmental timing of myogenesis. Proc Natl Acad Sci U S A 108(29):11936-41. [PubMed: 21730146] [MGI Ref ID J:174364]
Haldar M; Hancock JD; Coffin CM; Lessnick SL; Capecchi MR. 2007. A conditional mouse model of synovial sarcoma: insights into a myogenic origin. Cancer Cell 11(4):375-88. [PubMed: 17418413] [MGI Ref ID J:120967]
Harel I; Nathan E; Tirosh-Finkel L; Zigdon H; Guimaraes-Camboa N; Evans SM; Tzahor E. 2009. Distinct origins and genetic programs of head muscle satellite cells. Dev Cell 16(6):822-32. [PubMed: 19531353] [MGI Ref ID J:150129]
Hasson P; DeLaurier A; Bennett M; Grigorieva E; Naiche LA; Papaioannou VE; Mohun TJ; Logan MP. 2010. Tbx4 and tbx5 acting in connective tissue are required for limb muscle and tendon patterning. Dev Cell 18(1):148-56. [PubMed: 20152185] [MGI Ref ID J:157917]
High FA; Zhang M; Proweller A; Tu L; Parmacek MS; Pear WS; Epstein JA. 2007. An essential role for Notch in neural crest during cardiovascular development and smooth muscle differentiation. J Clin Invest 117(2):353-363. [PubMed: 17273555] [MGI Ref ID J:118039]
Hori K; Cholewa-Waclaw J; Nakada Y; Glasgow SM; Masui T; Henke RM; Wildner H; Martarelli B; Beres TM; Epstein JA; Magnuson MA; Macdonald RJ; Birchmeier C; Johnson JE. 2008. A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling. Genes Dev 22(2):166-78. [PubMed: 18198335] [MGI Ref ID J:130251]
Horner VL; Caspary T. 2011. Disrupted dorsal neural tube BMP signaling in the cilia mutant Arl13b hnn stems from abnormal Shh signaling. Dev Biol 355(1):43-54. [PubMed: 21539826] [MGI Ref ID J:173637]
Ismat FA; Xu J; Lu MM; Epstein JA. 2006. The neurofibromin GAP-related domain rescues endothelial but not neural crest development in Nf1 mice. J Clin Invest 116(9):2378-84. [PubMed: 16906226] [MGI Ref ID J:114455]
Jain R; Engleka KA; Rentschler SL; Manderfield LJ; Li L; Yuan L; Epstein JA. 2011. Cardiac neural crest orchestrates remodeling and functional maturation of mouse semilunar valves. J Clin Invest 121(1):422-30. [PubMed: 21157040] [MGI Ref ID J:171842]
Jarad G; Miner JH. 2009. The Pax3-Cre transgene exhibits a rostrocaudal gradient of expression in the skeletal muscle lineage. Genesis 47(1):1-6. [PubMed: 18942111] [MGI Ref ID J:144640]
Kahn J; Shwartz Y; Blitz E; Krief S; Sharir A; Breitel DA; Rattenbach R; Relaix F; Maire P; Rountree RB; Kingsley DM; Zelzer E. 2009. Muscle contraction is necessary to maintain joint progenitor cell fate. Dev Cell 16(5):734-43. [PubMed: 19460349] [MGI Ref ID J:148688]
Lagha M; Kormish JD; Rocancourt D; Manceau M; Epstein JA; Zaret KS; Relaix F; Buckingham ME. 2008. Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program. Genes Dev 22(13):1828-37. [PubMed: 18593883] [MGI Ref ID J:137423]
Levin MD; Lu MM; Petrenko NB; Hawkins BJ; Gupta TH; Lang D; Buckley PT; Jochems J; Liu F; Spurney CF; Yuan LJ; Jacobson JT; Brown CB; Huang L; Beermann F; Margulies KB; Madesh M; Eberwine JH; Epstein JA; Patel VV. 2009. Melanocyte-like cells in the heart and pulmonary veins contribute to atrial arrhythmia triggers. J Clin Invest 119(11):3420-36. [PubMed: 19855129] [MGI Ref ID J:154593]
Mathew SJ; Hansen JM; Merrell AJ; Murphy MM; Lawson JA; Hutcheson DA; Hansen MS; Angus-Hill M; Kardon G. 2011. Connective tissue fibroblasts and Tcf4 regulate myogenesis. Development 138(2):371-84. [PubMed: 21177349] [MGI Ref ID J:167712]
Mittal A; Pulina M; Hou SY; Astrof S. 2010. Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest. Mech Dev 127(9-12):472-84. [PubMed: 20807571] [MGI Ref ID J:165711]
Nelms BL; Pfaltzgraff ER; Labosky PA. 2011. Functional interaction between Foxd3 and Pax3 in cardiac neural crest development. Genesis 49(1):10-23. [PubMed: 21254333] [MGI Ref ID J:167976]
Park EJ; Watanabe Y; Smyth G; Miyagawa-Tomita S; Meyers E; Klingensmith J; Camenisch T; Buckingham M; Moon AM. 2008. An FGF autocrine loop initiated in second heart field mesoderm regulates morphogenesis at the arterial pole of the heart. Development 135(21):3599-610. [PubMed: 18832392] [MGI Ref ID J:143444]
Pietila I; Ellwanger K; Railo A; Jokela T; Barrantes Idel B; Shan J; Niehrs C; Vainio SJ. 2011. Secreted Wnt antagonist Dickkopf-1 controls kidney papilla development coordinated by Wnt-7b signalling. Dev Biol 353(1):50-60. [PubMed: 21354128] [MGI Ref ID J:173125]
Saifudeen Z; Dipp S; Stefkova J; Yao X; Lookabaugh S; El-Dahr SS. 2009. p53 regulates metanephric development. J Am Soc Nephrol 20(11):2328-37. [PubMed: 19729440] [MGI Ref ID J:166316]
Sato T; Rocancourt D; Marques L; Thorsteinsdottir S; Buckingham M. 2010. A Pax3/Dmrt2/Myf5 regulatory cascade functions at the onset of myogenesis. PLoS Genet 6(4):e1000897. [PubMed: 20368965] [MGI Ref ID J:159213]
Stoller JZ; Degenhardt KR; Huang L; Zhou DD; Lu MM; Epstein JA. 2008. Cre reporter mouse expressing a nuclear localized fusion of GFP and beta-galactosidase reveals new derivatives of Pax3-expressing precursors. Genesis 46(4):200-4. [PubMed: 18395835] [MGI Ref ID J:135149]
Tozer S; Bonnin MA; Relaix F; Di Savino S; Garcia-Villalba P; Coumailleau P; Duprez D. 2007. Involvement of vessels and PDGFB in muscle splitting during chick limb development. Development 134(14):2579-91. [PubMed: 17553906] [MGI Ref ID J:122753]
Varadkar P; Kraman M; Despres D; Ma G; Lozier J; McCright B. 2008. Notch2 is required for the proliferation of cardiac neural crest-derived smooth muscle cells. Dev Dyn 237(4):1144-52. [PubMed: 18330927] [MGI Ref ID J:132939]
Vasyutina E; Lenhard DC; Wende H; Erdmann B; Epstein JA; Birchmeier C. 2007. RBP-J (Rbpsuh) is essential to maintain muscle progenitor cells and to generate satellite cells. Proc Natl Acad Sci U S A 104(11):4443-8. [PubMed: 17360543] [MGI Ref ID J:120053]
Wu M; Li J; Engleka KA; Zhou B; Lu MM; Plotkin JB; Epstein JA. 2008. Persistent expression of Pax3 in the neural crest causes cleft palate and defective osteogenesis in mice. J Clin Invest 118(6):2076-87. [PubMed: 18483623] [MGI Ref ID J:137730]
Zhu Q; Whittemore SR; Devries WH; Zhao X; Kuypers NJ; Qiu M. 2011. Dorsally-derived oligodendrocytes in the spinal cord contribute to axonal myelination during development and remyelination following focal demyelination. Glia 59(11):1612-21. [PubMed: 21710609] [MGI Ref ID J:175450]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. Mating System Heterozygote x F1 (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Heterozygous for Pax3tm1(cre)Joe
Pairs /Price (US dollars $) Pair Genotype $294.50 B6129SF1/J (101043) x Heterozygous for Pax3tm1(cre)Joe $294.50 Heterozygous for Pax3tm1(cre)Joe x B6129SF1/J (101043) Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Heterozygous for Pax3tm1(cre)Joe
Pairs /Price (US dollars $) Pair Genotype $382.90 B6129SF1/J (101043) x Heterozygous for Pax3tm1(cre)Joe $382.90 Heterozygous for Pax3tm1(cre)Joe x B6129SF1/J (101043) Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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