Strain Name: |
B6;129-Pax3tm1(cre)Joe/J |
|---|---|
Stock Number: |
005549 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Pax3; Pax3tm1(cre)Joe; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x F1 (Female x Male) Species laboratory mouse Donating Investigator Jonathan Epstein, University of Pennsylvania Generation N?+6 (28-MAY-08) Strain Description
This strain expresses Cre recombinase from the endogenous Pax3 locus. Expression of the targeted gene product (mRNA and protein) mimics endogenous gene expression as detected by in situ hybridization and immunohistochemistry of homozygous embryos aged E12.5. No endogenous Pax3 gene product (protein) is detected in homozygotes and approximately one half of the endogenous gene product (protein) is detected in heterozygotes by Western blot analysis. Cre recombinase expression is detected in the dorsal neural tube and somites of E9 to 11.5 embryos and in the cardiac neural crest cells and colonic epithelia of E11.5 embryos. Recombination occurs in neural crest and somite derivatives of later gestation embryos. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. At age E13.5 homozygous embryos display severe cardiac and neural tube defects (exencephaly), absent limb musculature and reduced or absent dorsal root ganglia. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Half of the heterozygous mice exhibit a white belly spot. The phenotype exhibited by this mutant strain is similar to the phenotype observed in mice carrying the spontaneous splotch mutant allele of Pax3. This mutant mouse strain represents a model that may be useful in studies of development and lineage mapping of neural crest and somite derivatives.Strain Development
A targeting vector containing Cre coding sequence, a loxP site flanked PGK-neo cassette and a PGK-herpes simplex virus thymidine kinase gene was used to disrupt most of exon 1 including the initiation codon and 69 bp of the 5' flanking region. The endogenous Pax3 promoter drives expression of the Cre recombinase through the in-frame insertion of the Cre coding sequence to the first exon of the Pax3 gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice once and are maintained on a mixed B6;129 background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Pax3tm1(cre)Joe | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jonathan A Epstein | ||
| Common Name(s) | Pax3-Cre; Pax3Cre; Pax3Cre-KI; | ||
| Mutation Made By | Kurt Engleka, University of Pennsylvania | ||
| Site of Expression | dorsal neural tube and somites of embryonic day 9-11.5 embryos and in the cardiac neural crest cells and colonic epithelia of embryonic day 11.5 embryos | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Pax3, paired box gene 3 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CDHS; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; Pax-3; Sp; WS1; splotch; | ||
| Molecular Note | A targeting vector was desinged to insert the cre recombinase cDNA followed by a stop codon and a polyA signal in place of exon 1 of the endogenous locus. [MGI Ref ID J:96431] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Pax3tm1(cre)Joe
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Pax3
000311 B6-Pax3Sp.Cg-N/J 000565 C57BL/6J-Pax3Sp-d/J 002469 C57BL/6J-Pax3Sp/J 002902 STOCK Pax3Sp Mlphln/J View Strains carrying other alleles of Pax3 (4 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects (multiple)
Limb Patterning Defects
Neural Tube Defects
Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Neural Tube Defects
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLang D; Lu MM; Huang L; Engleka KA; Zhang M; Chu EY; Lipner S; Skoultchi A; Millar SE; Epstein JA. 2005. Pax3 functions at a nodal point in melanocyte stem cell differentiation. Nature 433(7028):884-7. [PubMed: 15729346] [MGI Ref ID J:96431]
Price and Supply Information
| Strain Name: | B6;129-Pax3tm1(cre)Joe/J |
| Stock Number: | 005549 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Heterozygous for Pax3tm1(cre)Joe | |||
| Pair | $329.10 | B6129SF1/J (101043) x Heterozygous for Pax3tm1(cre)Joe | |||
| Pair | $329.10 | Heterozygous for Pax3tm1(cre)Joe x B6129SF1/J (101043) | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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