Strain Name:

STOCK Rac1tm1Djk/J

Stock Number:

005550

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Description

Strain Information

Former Names STOCK Rac1tm1Glog/J    (Changed: 13-FEB-06 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   04-JUN-14
Specieslaboratory mouse
Generation?+N1p (02-DEC-13)
Generation Definitions
 
Donating Investigator David J. Kwiatkowski,   Brigham and Women's Hospital

Description
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of neutrophil function.

When bred to a strain expressing a tamoxifen inducible Cre recombinase specific to keratinocytes(see Stock No. 005107 for example), this mutant mouse strain may be useful in studies of stem cell renewal in the epidermis.

Development
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a vector expressing Cre to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting mice were then crossed to a mixed background (129S4/SvJae, BALB/c, C57BL/6).

Control Information

  Control
   None Available
   See control note: This strain is a mixture of 129S4/SvJae, BALB/c, C57BL/6 strains. There is no appropriate control available for this strain.
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Rac1tm1Djk/Rac1tm1.1Djk Lyz2tm1(cre)Ifo/Lyz2+

        involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6   (conditional)
  • immune system phenotype
  • decreased acute inflammation
    • 3 hrs after i.p. injection of sodium periodate, mutant mice display impaired neutrophil chemotaxis and in vivo recruitment to sites of acute inflammation relative to wild-type controls   (MGI Ref ID J:83457)
  • decreased leukocyte cell number
    • 3 hrs after induction of peritonitis by sodium periodate injection, circulating leukocyte counts are not significantly increased, unlike in wild-type controls   (MGI Ref ID J:83457)
    • decreased neutrophil cell number
      • 3 hrs after induction of peritonitis, only a small increase in peripheral neutrophil counts is observed, unlike in wild-type controls where circulating neutrophil counts are increased by >3-fold   (MGI Ref ID J:83457)
  • impaired neutrophil chemotaxis
    • in vitro, mutant bone marrow neutrophils show a ~50% reduction in fMLP-induced chemotaxis relative to wild-type neutrophils both at 1 and 10 uM fMLP   (MGI Ref ID J:83457)
    • mutant neutrophils show a significant reduction in fMLP-induced F-actin formation, with a slower rate of actin polymerization relative to wild-type neutrophils   (MGI Ref ID J:83457)
    • however, both PMA- and fMLP-stimulated mutant bone marrow neutrophils exhibit normal superoxide production relative to wild-type neutrophils   (MGI Ref ID J:83457)
  • impaired neutrophil recruitment
    • 3 hrs after sodium periodate injection into the peritoneum, mutant mice exhibit a >50% reduction in neutrophil accumulation at the site of inflammation relative to wild-type controls   (MGI Ref ID J:83457)
  • hematopoietic system phenotype
  • decreased leukocyte cell number
    • 3 hrs after induction of peritonitis by sodium periodate injection, circulating leukocyte counts are not significantly increased, unlike in wild-type controls   (MGI Ref ID J:83457)
    • decreased neutrophil cell number
      • 3 hrs after induction of peritonitis, only a small increase in peripheral neutrophil counts is observed, unlike in wild-type controls where circulating neutrophil counts are increased by >3-fold   (MGI Ref ID J:83457)
  • impaired neutrophil chemotaxis
    • in vitro, mutant bone marrow neutrophils show a ~50% reduction in fMLP-induced chemotaxis relative to wild-type neutrophils both at 1 and 10 uM fMLP   (MGI Ref ID J:83457)
    • mutant neutrophils show a significant reduction in fMLP-induced F-actin formation, with a slower rate of actin polymerization relative to wild-type neutrophils   (MGI Ref ID J:83457)
    • however, both PMA- and fMLP-stimulated mutant bone marrow neutrophils exhibit normal superoxide production relative to wild-type neutrophils   (MGI Ref ID J:83457)
  • impaired neutrophil recruitment
    • 3 hrs after sodium periodate injection into the peritoneum, mutant mice exhibit a >50% reduction in neutrophil accumulation at the site of inflammation relative to wild-type controls   (MGI Ref ID J:83457)
  • cellular phenotype
  • impaired neutrophil chemotaxis
    • in vitro, mutant bone marrow neutrophils show a ~50% reduction in fMLP-induced chemotaxis relative to wild-type neutrophils both at 1 and 10 uM fMLP   (MGI Ref ID J:83457)
    • mutant neutrophils show a significant reduction in fMLP-induced F-actin formation, with a slower rate of actin polymerization relative to wild-type neutrophils   (MGI Ref ID J:83457)
    • however, both PMA- and fMLP-stimulated mutant bone marrow neutrophils exhibit normal superoxide production relative to wild-type neutrophils   (MGI Ref ID J:83457)

Rac1tm1Djk/Rac1tm1Djk Tg(KRT14-cre/ERT)20Efu/0

        involves: 129S4/SvJae * C57BL/6 * CD-1   (conditional)
  • endocrine/exocrine gland phenotype
  • enlarged sebaceous gland
    • 7 to 9 days after tamoxifen treatment sebaceous glands are enlarged and disorganized   (MGI Ref ID J:99995)
    • an early increase in the number of terminally differentiated sebocytes is followed by progressive sebocyte loss   (MGI Ref ID J:99995)
  • integument phenotype
  • abnormal epidermal layer morphology
    • 3 to 5 days after tamoxifen treatment the interfollicular epidermis is thickened with an increase in cell numbers in the living and cornified layers and the infundibulum at the junction of the interfollicular epidermis and hair follicle is expanded   (MGI Ref ID J:99995)
    • 3 to 5 days after tamoxifen treatment the size of hemidesmosomes is reduced and later the numbers are reduced and those remaining are rudimentary   (MGI Ref ID J:99995)
    • 11 to 15 days after tamoxifen treatment partial to complete loss of viable interfollicular epidermal cell layers is seen   (MGI Ref ID J:99995)
    • abnormal epidermis stratum basale morphology
      • 7 to 9 days after tamoxifen treatment the basal layer is disorganized and has fewer, larger cells   (MGI Ref ID J:99995)
  • abnormal hair cycle
    • pronounced defects in hair cycle are seen   (MGI Ref ID J:99995)
  • abnormal hair follicle morphology
    • 11 to 15 days after tamoxifen treatment the hair follicle is reduced in size and the infundibulum degenerates into cysts   (MGI Ref ID J:99995)
    • the hair bulb is reduced and then absent following tamoxifen treatment   (MGI Ref ID J:99995)
    • depletion of stem cells is detected by the decreased expression of 3 hair follicle bulge markers   (MGI Ref ID J:99995)
  • abnormal keratinocyte physiology
    • in culture, treatment of keratinocytes with tamoxifen blocks clonal growth   (MGI Ref ID J:99995)
  • abnormal skin cell number
    • 3 to 5 days after tamoxifen treatment increased proliferation is seen in the epidermis, however from day 7 on proliferation is reduced   (MGI Ref ID J:99995)
  • enlarged sebaceous gland
    • 7 to 9 days after tamoxifen treatment sebaceous glands are enlarged and disorganized   (MGI Ref ID J:99995)
    • an early increase in the number of terminally differentiated sebocytes is followed by progressive sebocyte loss   (MGI Ref ID J:99995)
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Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rac1tm1Djk
Allele Name targeted mutation 1, David J Kwiatkowski
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Rac1c; Rac1flox; Rac1tm1Glog;
Mutation Made By David Kwiatkowski,   Brigham and Women's Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Rac1, RAS-related C3 botulinum substrate 1
Chromosome 5
Gene Common Name(s) AL023026; D5Ertd559e; DNA segment, Chr 5, ERATO Doi 559, expressed; Rac-1; TC-25; expressed sequence AL023026; p21-Rac1;
Molecular Note Exon 1 was left flanked by single loxP sites after the excision of floxed neo cassette. [MGI Ref ID J:83457]

Genotyping

Genotyping Information

Genotyping Protocols

Rac1tm1Djk STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Glogauer M; Marchal CC; Zhu F; Worku A; Clausen BE; Foerster I; Marks P; Downey GP; Dinauer M; Kwiatkowski DJ. 2003. Rac1 deletion in mouse neutrophils has selective effects on neutrophil functions. J Immunol 170(11):5652-7. [PubMed: 12759446]  [MGI Ref ID J:83457]

Additional References

Rac1tm1Djk related

Benitah SA; Frye M; Glogauer M; Watt FM. 2005. Stem cell depletion through epidermal deletion of Rac1. Science 309(5736):933-5. [PubMed: 16081735]  [MGI Ref ID J:99995]

Benitah SA; Watt FM. 2007. Epidermal deletion of Rac1 causes stem cell depletion, irrespective of whether deletion occurs during embryogenesis or adulthood. J Invest Dermatol 127(6):1555-7. [PubMed: 17301832]  [MGI Ref ID J:121550]

Bongmba OY; Martinez LA; Elhardt ME; Butler K; Tejada-Simon MV. 2011. Modulation of dendritic spines and synaptic function by Rac1: A possible link to Fragile X syndrome pathology. Brain Res 1399:79-95. [PubMed: 21645877]  [MGI Ref ID J:174070]

Camerer E; Barker A; Duong DN; Ganesan R; Kataoka H; Cornelissen I; Darragh MR; Hussain A; Zheng YW; Srinivasan Y; Brown C; Xu SM; Regard JB; Lin CY; Craik CS; Kirchhofer D; Coughlin SR. 2010. Local protease signaling contributes to neural tube closure in the mouse embryo. Dev Cell 18(1):25-38. [PubMed: 20152175]  [MGI Ref ID J:157446]

Cancelas JA; Jansen M; Williams DA. 2006. The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization. Exp Hematol 34(8):976-85. [PubMed: 16863904]  [MGI Ref ID J:111908]

Cancelas JA; Lee AW; Prabhakar R; Stringer KF; Zheng Y; Williams DA. 2005. Rac GTPases differentially integrate signals regulating hematopoietic stem cell localization. Nat Med 11(8):886-91. [PubMed: 16025125]  [MGI Ref ID J:110380]

Castilho RM; Squarize CH; Leelahavanichkul K; Zheng Y; Bugge T; Gutkind JS. 2010. Rac1 is required for epithelial stem cell function during dermal and oral mucosal wound healing but not for tissue homeostasis in mice. PLoS One 5(5):e10503. [PubMed: 20463891]  [MGI Ref ID J:160840]

Castilho RM; Squarize CH; Patel V; Millar SE; Zheng Y; Molinolo A; Gutkind JS. 2007. Requirement of Rac1 distinguishes follicular from interfollicular epithelial stem cells. Oncogene 26(35):5078-85. [PubMed: 17334398]  [MGI Ref ID J:141665]

Chauhan BK; Disanza A; Choi SY; Faber SC; Lou M; Beggs HE; Scita G; Zheng Y; Lang RA. 2009. Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination. Development 136(21):3657-67. [PubMed: 19820184]  [MGI Ref ID J:153957]

Chauhan BK; Lou M; Zheng Y; Lang RA. 2011. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108(45):18289-94. [PubMed: 22021442]  [MGI Ref ID J:180120]

Chen L; Liao G; Waclaw RR; Burns KA; Linquist D; Campbell K; Zheng Y; Kuan CY. 2007. Rac1 controls the formation of midline commissures and the competency of tangential migration in ventral telencephalic neurons. J Neurosci 27(14):3884-93. [PubMed: 17409253]  [MGI Ref ID J:121201]

Chen L; Melendez J; Campbell K; Kuan CY; Zheng Y. 2009. Rac1 deficiency in the forebrain results in neural progenitor reduction and microcephaly. Dev Biol 325(1):162-70. [PubMed: 19007770]  [MGI Ref ID J:143541]

Dietz DM; Sun H; Lobo MK; Cahill ME; Chadwick B; Gao V; Koo JW; Mazei-Robison MS; Dias C; Maze I; Damez-Werno D; Dietz KC; Scobie KN; Ferguson D; Christoffel D; Ohnishi Y; Hodes GE; Zheng Y; Neve RL; Hahn KM; Russo SJ; Nestler EJ. 2012. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci 15(6):891-6. [PubMed: 22522400]  [MGI Ref ID J:191368]

Filippi MD; Szczur K; Harris CE; Berclaz PY. 2007. Rho GTPase Rac1 is critical for neutrophil migration into the lung. Blood 109(3):1257-64. [PubMed: 16990606]  [MGI Ref ID J:144399]

Ghiaur G; Ferkowicz MJ; Milsom MD; Bailey J; Witte D; Cancelas JA; Yoder MC; Williams DA. 2008. Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells. Blood 111(7):3313-21. [PubMed: 18083846]  [MGI Ref ID J:133470]

Goldberg SR; Georgiou J; Glogauer M; Grynpas MD. 2012. A 3D scanning confocal imaging method measures pit volume and captures the role of Rac in osteoclast function. Bone 51(1):145-52. [PubMed: 22561898]  [MGI Ref ID J:186186]

Golden SA; Christoffel DJ; Heshmati M; Hodes GE; Magida J; Davis K; Cahill ME; Dias C; Ribeiro E; Ables JL; Kennedy PJ; Robison AJ; Gonzalez-Maeso J; Neve RL; Turecki G; Ghose S; Tamminga CA; Russo SJ. 2013. Epigenetic regulation of RAC1 induces synaptic remodeling in stress disorders and depression. Nat Med 19(3):337-44. [PubMed: 23416703]  [MGI Ref ID J:196296]

Grimsley-Myers CM; Sipe CW; Geleoc GS; Lu X. 2009. The small GTPase Rac1 regulates auditory hair cell morphogenesis. J Neurosci 29(50):15859-69. [PubMed: 20016102]  [MGI Ref ID J:157099]

Grimsley-Myers CM; Sipe CW; Wu DK; Lu X. 2012. Redundant functions of Rac GTPases in inner ear morphogenesis. Dev Biol 362(2):172-86. [PubMed: 22182523]  [MGI Ref ID J:180774]

Gu Y; Filippi MD; Cancelas JA; Siefring JE; Williams EP; Jasti AC; Harris CE; Lee AW; Prabhakar R; Atkinson SJ; Kwiatkowski DJ; Williams DA. 2003. Hematopoietic cell regulation by Rac1 and Rac2 guanosine triphosphatases. Science 302(5644):445-9. [PubMed: 14564009]  [MGI Ref ID J:86814]

Guo F; Cancelas JA; Hildeman D; Williams DA; Zheng Y. 2008. Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development. Blood 112(5):1767-75. [PubMed: 18579797]  [MGI Ref ID J:138889]

Guo L; Moon C; Niehaus K; Zheng Y; Ratner N. 2012. Rac1 controls Schwann cell myelination through cAMP and NF2/merlin. J Neurosci 32(48):17251-61. [PubMed: 23197717]  [MGI Ref ID J:193053]

Haruta M; Bush RA; Kjellstrom S; Vijayasarathy C; Zeng Y; Le YZ; Sieving PA. 2009. Depleting Rac1 in mouse rod photoreceptors protects them from photo-oxidative stress without affecting their structure or function. Proc Natl Acad Sci U S A 106(23):9397-402. [PubMed: 19470639]  [MGI Ref ID J:150082]

Ho E; Dagnino L. 2012. Epidermal growth factor induction of front-rear polarity and migration in keratinocytes is mediated by integrin-linked kinase and ELMO2. Mol Biol Cell 23(3):492-502. [PubMed: 22160594]  [MGI Ref ID J:197014]

Hunziker L; Aznar Benitah S; Braun KM; Jensen K; McNulty K; Butler C; Potton E; Nye E; Boyd R; Laurent G; Glogauer M; Wright NA; Watt FM; Janes SM. 2011. Rac1 deletion causes thymic atrophy. PLoS One 6(4):e19292. [PubMed: 21559396]  [MGI Ref ID J:172344]

Kalfa TA; Pushkaran S; Mohandas N; Hartwig JH; Fowler VM; Johnson JF; Joiner CH; Williams DA; Zheng Y. 2006. Rac GTPases regulate the morphology and deformability of the erythrocyte cytoskeleton. Blood 108(12):3637-45. [PubMed: 16882712]  [MGI Ref ID J:140447]

Kalfa TA; Pushkaran S; Zhang X; Johnson JF; Pan D; Daria D; Geiger H; Cancelas JA; Williams DA; Zheng Y. 2010. Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen. Haematologica 95(1):27-35. [PubMed: 20065081]  [MGI Ref ID J:162051]

Karlsson T; Glogauer M; Ellen RP; Loitto VM; Magnusson KE; Magalhaes MA. 2011. Aquaporin 9 phosphorylation mediates membrane localization and neutrophil polarization. J Leukoc Biol 90(5):963-73. [PubMed: 21873454]  [MGI Ref ID J:178235]

Koh AL; Sun CX; Zhu F; Glogauer M. 2005. The role of Rac1 and Rac2 in bacterial killing. Cell Immunol 235(2):92-7. [PubMed: 16157315]  [MGI Ref ID J:107943]

Konstantinidis DG; Pushkaran S; Johnson JF; Cancelas JA; Manganaris S; Harris CE; Williams DA; Zheng Y; Kalfa TA. 2012. Signaling and cytoskeletal requirements in erythroblast enucleation. Blood 119(25):6118-27. [PubMed: 22461493]  [MGI Ref ID J:186731]

Lane SW; De Vita S; Alexander KA; Karaman R; Milsom MD; Dorrance AM; Purdon A; Louis L; Bouxsein ML; Williams DA. 2012. Rac signaling in osteoblastic cells is required for normal bone development but is dispensable for hematopoietic development. Blood 119(3):736-44. [PubMed: 22123845]  [MGI Ref ID J:181778]

Li J; Zhu H; Shen E; Wan L; Arnold JM; Peng T. 2010. Deficiency of rac1 blocks NADPH oxidase activation, inhibits endoplasmic reticulum stress, and reduces myocardial remodeling in a mouse model of type 1 diabetes. Diabetes 59(8):2033-42. [PubMed: 20522592]  [MGI Ref ID J:169350]

Liu S; Kapoor M; Leask A. 2009. Rac1 expression by fibroblasts is required for tissue repair in vivo. Am J Pathol 174(5):1847-56. [PubMed: 19349358]  [MGI Ref ID J:147970]

Ma J; Wang Y; Zheng D; Wei M; Xu H; Peng T. 2013. Rac1 signalling mediates doxorubicin-induced cardiotoxicity through both reactive oxygen species-dependent and -independent pathways. Cardiovasc Res 97(1):77-87. [PubMed: 23027656]  [MGI Ref ID J:210068]

Maddala R; Chauhan BK; Walker C; Zheng Y; Robinson ML; Lang RA; Rao PV. 2011. Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival. Dev Biol 360(1):30-43. [PubMed: 21945075]  [MGI Ref ID J:178484]

Manchanda PK; Jones GN; Lee AA; Pringle DR; Zhang M; Yu L; La Perle KM; Kirschner LS. 2013. Rac1 is required for Prkar1a-mediated Nf2 suppression in Schwann cell tumors. Oncogene 32(30):3491-9. [PubMed: 23045281]  [MGI Ref ID J:199975]

Martin H; Mali RS; Ma P; Chatterjee A; Ramdas B; Sims E; Munugalavadla V; Ghosh J; Mattingly RR; Visconte V; Tiu RV; Vlaar CP; Dharmawardhane S; Kapur R. 2013. Pak and Rac GTPases promote oncogenic KIT-induced neoplasms. J Clin Invest 123(10):4449-63. [PubMed: 24091327]  [MGI Ref ID J:203981]

Mizukawa B; Wei J; Shrestha M; Wunderlich M; Chou FS; Griesinger A; Harris CE; Kumar AR; Zheng Y; Williams DA; Mulloy JC. 2011. Inhibition of Rac GTPase signaling and downstream prosurvival Bcl-2 proteins as combination targeted therapy in MLL-AF9 leukemia. Blood 118(19):5235-45. [PubMed: 21940819]  [MGI Ref ID J:178886]

Mondal S; Ghosh-Roy S; Loison F; Li Y; Jia Y; Harris C; Williams DA; Luo HR. 2011. PTEN negatively regulates engulfment of apoptotic cells by modulating activation of Rac GTPase. J Immunol 187(11):5783-94. [PubMed: 22043008]  [MGI Ref ID J:179722]

Murthy S; Adamcakova-Dodd A; Perry SS; Tephly LA; Keller RM; Metwali N; Meyerholz DK; Wang Y; Glogauer M; Thorne PS; Carter AB. 2009. Modulation of reactive oxygen species by Rac1 or catalase prevents asbestos-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 297(5):L846-55. [PubMed: 19684199]  [MGI Ref ID J:154331]

Murthy S; Ryan AJ; Carter AB. 2012. SP-1 regulation of MMP-9 expression requires Ser586 in the PEST domain. Biochem J 445(2):229-36. [PubMed: 22519702]  [MGI Ref ID J:188079]

Saci A; Cantley LC; Carpenter CL. 2011. Rac1 regulates the activity of mTORC1 and mTORC2 and controls cellular size. Mol Cell 42(1):50-61. [PubMed: 21474067]  [MGI Ref ID J:172668]

Satoh M; Ogita H; Takeshita K; Mukai Y; Kwiatkowski DJ; Liao JK. 2006. Requirement of Rac1 in the development of cardiac hypertrophy. Proc Natl Acad Sci U S A 103(19):7432-7. [PubMed: 16651530]  [MGI Ref ID J:109582]

Sawada N; Salomone S; Kim HH; Kwiatkowski DJ; Liao JK. 2008. Regulation of endothelial nitric oxide synthase and postnatal angiogenesis by Rac1. Circ Res 103(4):360-8. [PubMed: 18599867]  [MGI Ref ID J:152655]

Shan L; Li J; Wei M; Ma J; Wan L; Zhu W; Li Y; Zhu H; Arnold JM; Peng T. 2010. Disruption of Rac1 signaling reduces ischemia-reperfusion injury in the diabetic heart by inhibiting calpain. Free Radic Biol Med 49(11):1804-14. [PubMed: 20883775]  [MGI Ref ID J:166075]

Shen E; Li Y; Li Y; Shan L; Zhu H; Feng Q; Arnold JM; Peng T. 2009. Rac1 is required for cardiomyocyte apoptosis during hyperglycemia. Diabetes 58(10):2386-95. [PubMed: 19592621]  [MGI Ref ID J:154395]

Sima C; Gastfreund S; Sun C; Glogauer M. 2014. Rac-null leukocytes are associated with increased inflammation-mediated alveolar bone loss. Am J Pathol 184(2):472-82. [PubMed: 24269593]  [MGI Ref ID J:205191]

Spencer NY; Yan Z; Boudreau RL; Zhang Y; Luo M; Li Q; Tian X; Shah AM; Davisson RL; Davidson B; Banfi B; Engelhardt JF. 2011. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 286(11):8977-87. [PubMed: 21212270]  [MGI Ref ID J:170529]

Spencer NY; Zhou W; Li Q; Zhang Y; Luo M; Yan Z; Lynch TJ; Abbott D; Banfi B; Engelhardt JF. 2013. Hepatocytes produce TNF-alpha following hypoxia-reoxygenation and liver ischemia-reperfusion in a NADPH oxidase- and c-Src-dependent manner. Am J Physiol Gastrointest Liver Physiol 305(1):G84-94. [PubMed: 23639811]  [MGI Ref ID J:202785]

Takashima S; Kanatsu-Shinohara M; Tanaka T; Takehashi M; Morimoto H; Shinohara T. 2011. Rac Mediates Mouse Spermatogonial Stem Cell Homing to Germline Niches by Regulating Transmigration through the Blood-Testis Barrier. Cell Stem Cell 9(5):463-75. [PubMed: 22056142]  [MGI Ref ID J:177839]

Tan W; Palmby TR; Gavard J; Amornphimoltham P; Zheng Y; Gutkind JS. 2008. An essential role for Rac1 in endothelial cell function and vascular development. FASEB J 22(6):1829-38. [PubMed: 18245172]  [MGI Ref ID J:136526]

Thomas EK; Cancelas JA; Chae HD; Cox AD; Keller PJ; Perrotti D; Neviani P; Druker BJ; Setchell KD; Zheng Y; Harris CE; Williams DA. 2007. Rac guanosine triphosphatases represent integrating molecular therapeutic targets for BCR-ABL-induced myeloproliferative disease. Cancer Cell 12(5):467-78. [PubMed: 17996650]  [MGI Ref ID J:127321]

Thomas PS; Kim J; Nunez S; Glogauer M; Kaartinen V. 2010. Neural crest cell-specific deletion of Rac1 results in defective cell-matrix interactions and severe craniofacial and cardiovascular malformations. Dev Biol 340(2):613-25. [PubMed: 20184871]  [MGI Ref ID J:160252]

Utomo A; Cullere X; Glogauer M; Swat W; Mayadas TN. 2006. Vav proteins in neutrophils are required for FcgammaR-mediated signaling to Rac GTPases and nicotinamide adenine dinucleotide phosphate oxidase component p40(phox). J Immunol 177(9):6388-97. [PubMed: 17056570]  [MGI Ref ID J:140509]

Vidali L; Chen F; Cicchetti G; Ohta Y; Kwiatkowski DJ. 2006. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor. Mol Biol Cell 17(5):2377-90. [PubMed: 16525021]  [MGI Ref ID J:112526]

Wang G; Woods A; Agoston H; Ulici V; Glogauer M; Beier F. 2007. Genetic ablation of Rac1 in cartilage results in chondrodysplasia. Dev Biol 306(2):612-23. [PubMed: 17467682]  [MGI Ref ID J:122563]

Wang QQ; Li H; Oliver T; Glogauer M; Guo J; He YW. 2008. Integrin beta1 regulates phagosome maturation in macrophages through Rac expression. J Immunol 180(4):2419-28. [PubMed: 18250451]  [MGI Ref ID J:131992]

Wu X; Tu X; Joeng KS; Hilton MJ; Williams DA; Long F. 2008. Rac1 activation controls nuclear localization of beta-catenin during canonical Wnt signaling. Cell 133(2):340-53. [PubMed: 18423204]  [MGI Ref ID J:145305]

Yan J; Chen S; Zhang Y; Li X; Li Y; Wu X; Yuan J; Robling AG; Karpur R; Chan RJ; Yang FC. 2008. Rac1 mediates the osteoclast gains-in-function induced by haploinsufficiency of Nf1. Hum Mol Genet 17(7):936-48. [PubMed: 18089636]  [MGI Ref ID J:132466]

Zhang H; Sun C; Glogauer M; Bokoch GM. 2009. Human neutrophils coordinate chemotaxis by differential activation of Rac1 and Rac2. J Immunol 183(4):2718-28. [PubMed: 19625648]  [MGI Ref ID J:151789]

Zhu H; Shan L; Peng T. 2009. Rac1 mediates sex difference in cardiac tumor necrosis factor-alpha expression via NADPH oxidase-ERK1/2/p38 MAPK pathway in endotoxemia. J Mol Cell Cardiol 47(2):264-74. [PubMed: 19450605]  [MGI Ref ID J:151343]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   04-JUN-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Rac1tm1Djk  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Rac1tm1Djk x Homozygous for Rac1tm1Djk  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Rac1tm1Djk  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Rac1tm1Djk x Homozygous for Rac1tm1Djk  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   None Available
   See control note: This strain is a mixture of 129S4/SvJae, BALB/c, C57BL/6 strains. There is no appropriate control available for this strain.
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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