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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J (Changed: 23-NOV-05 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+F17 (27-NOV-11)
Generation DefinitionsDonating Investigator Andras Nagy, Mount Sinai Hospital Description
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the Cre-transgenic line and the doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.Of note, mutant mice are also available on a C57BL/6J genetic background (see Stock No. 005670).
Development
A targeting vector containing a loxP-flanked phosphoglycerate kinase-neomycin resistance gene-polyadenylation stop sequence (PGK-neo-pA) fragment and a downstream reverse tetracycline-controlled transactivator gene-internal ribosomal entry site-enhanced green fluorescent protein gene-polyadenylation stop sequence (rtTA-IRES-EGFP-pA) fusion protein was inserted between exon 1 and 2 of the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with ICR morulae and the resulting chimeric males were backcrossed for germ-line transmission to ICR females. Heterozygous offspring were intercrossed to produce wild type, heterozygotes, and homozygotes.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andras Nagy | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(rtTA)Nagy; Gt(ROSA)26Sortm1Nagy; ROSA26-rtTA; ROSA26-rtTA-IRES-EGFP; rtTAflox; | ||
| Mutation Made By | Gusztav Belteki and Jody Haigh, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. [MGI Ref ID J:80963] [MGI Ref ID J:99607] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy, Separated PCR
ROSA26 Generic GFP, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Belteki G; Haigh J; Kabacs N; Haigh K; Sison K; Costantini F; Whitsett J; Quaggin SE; Nagy A. 2005. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res 33(5):e51. [PubMed: 15784609] [MGI Ref ID J:99607]
Additional References
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy relatedDrogat B; Kalucka J; Gutierrez L; Hammad H; Goossens S; Farhang Ghahremani M; Bartunkova S; Haigh K; Deswarte K; Nyabi O; Naessens M; Ferrara N; Klingmuller U; Lambrecht BN; Nagy A; Philipsen S; Haigh JJ. 2010. Vegf regulates embryonic erythroid development through Gata1 modulation. Blood 116(12):2141-51. [PubMed: 20554972] [MGI Ref ID J:164506]
El Andaloussi A; Graves S; Meng F; Mandal M; Mashayekhi M; Aifantis I. 2006. Hedgehog signaling controls thymocyte progenitor homeostasis and differentiation in the thymus. Nat Immunol 7(4):418-26. [PubMed: 16518394] [MGI Ref ID J:112555]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Grachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145] [MGI Ref ID J:173930]
Gupte VV; Ramasamy SK; Reddy R; Lee J; Weinreb PH; Violette SM; Guenther A; Warburton D; Driscoll B; Minoo P; Bellusci S. 2009. Overexpression of fibroblast growth factor-10 during both inflammatory and fibrotic phases attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Respir Crit Care Med 180(5):424-36. [PubMed: 19498056] [MGI Ref ID J:167964]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2010. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem :. [PubMed: 21136430] [MGI Ref ID J:171769]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2011. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem 112(2):653-65. [PubMed: 21268087] [MGI Ref ID J:168061]
Jeansson M; Gawlik A; Anderson G; Li C; Kerjaschki D; Henkelman M; Quaggin SE. 2011. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury. J Clin Invest 121(6):2278-89. [PubMed: 21606590] [MGI Ref ID J:174022]
Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91. [PubMed: 21672919] [MGI Ref ID J:174960]
Maes C; Goossens S; Bartunkova S; Drogat B; Coenegrachts L; Stockmans I; Moermans K; Nyabi O; Haigh K; Naessens M; Haenebalcke L; Tuckermann JP; Tjwa M; Carmeliet P; Mandic V; David JP; Behrens A; Nagy A; Carmeliet G; Haigh JJ. 2010. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J 29(2):424-41. [PubMed: 20010698] [MGI Ref ID J:156474]
Moriyama M; Fukuhara T; Britschgi M; He Y; Narasimhan R; Villeda S; Molina H; Huber BT; Holers M; Wyss-Coray T. 2011. Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis. J Neurosci 31(11):3981-3989. [PubMed: 21411641] [MGI Ref ID J:170456]
Noah TK; Kazanjian A; Whitsett J; Shroyer NF. 2010. SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Exp Cell Res 316(3):452-65. [PubMed: 19786015] [MGI Ref ID J:156638]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Parsa S; Kuremoto K; Seidel K; Tabatabai R; Mackenzie B; Yamaza T; Akiyama K; Branch J; Koh CJ; Al Alam D; Klein OD; Bellusci S. 2010. Signaling by FGFR2b controls the regenerative capacity of adult mouse incisors. Development 137(22):3743-52. [PubMed: 20978072] [MGI Ref ID J:167068]
Parsa S; Ramasamy SK; De Langhe S; Gupte VV; Haigh JJ; Medina D; Bellusci S. 2008. Terminal end bud maintenance in mammary gland is dependent upon FGFR2b signaling. Dev Biol 317(1):121-31. [PubMed: 18381212] [MGI Ref ID J:136174]
Pi M; Chen L; Huang M; Luo Q; Quarles LD. 2008. Parathyroid-specific interaction of the calcium-sensing receptor and G alpha q. Kidney Int 74(12):1548-56. [PubMed: 18813283] [MGI Ref ID J:162715]
Sala FG; Del Moral PM; Tiozzo C; Alam DA; Warburton D; Grikscheit T; Veltmaat JM; Bellusci S. 2011. FGF10 controls the patterning of the tracheal cartilage rings via Shh. Development 138(2):273-82. [PubMed: 21148187] [MGI Ref ID J:167739]
Srinivas S; Watanabe T; Lin CS; William CM; Tanabe Y; Jessell TM; Costantini F. 2001. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1(1):4. [PubMed: 11299042] [MGI Ref ID J:80963]
Tang S; Snider P; Firulli AB; Conway SJ. 2010. Trigenic neural crest-restricted Smad7 over-expression results in congenital craniofacial and cardiovascular defects. Dev Biol 344(1):233-47. [PubMed: 20457144] [MGI Ref ID J:163879]
Vega-Hernandez M; Kovacs A; De Langhe S; Ornitz DM. 2011. FGF10/FGFR2b signaling is essential for cardiac fibroblast development and growth of the myocardium. Development 138(15):3331-40. [PubMed: 21750042] [MGI Ref ID J:175538]
Volckaert T; Dill E; Campbell A; Tiozzo C; Majka S; Bellusci S; De Langhe SP. 2011. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J Clin Invest 121(11):4409-19. [PubMed: 21985786] [MGI Ref ID J:178444]
Wang J; Greene SB; Bonilla-Claudio M; Tao Y; Zhang J; Bai Y; Huang Z; Black BL; Wang F; Martin JF. 2010. Bmp signaling regulates myocardial differentiation from cardiac progenitors through a MicroRNA-mediated mechanism. Dev Cell 19(6):903-12. [PubMed: 21145505] [MGI Ref ID J:169047]
Wehn AK; Chapman DL. 2010. Tbx18 and Tbx15 null-like phenotypes in mouse embryos expressing Tbx6 in somitic and lateral plate mesoderm. Dev Biol 347(2):404-13. [PubMed: 20832395] [MGI Ref ID J:166775]
Wehn AK; Gallo PH; Chapman DL. 2009. Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element. Genesis 47(5):309-313. [PubMed: 19298012] [MGI Ref ID J:148512]
Xu K; Nieuwenhuis E; Cohen BL; Wang W; Canty AJ; Danska JS; Coultas L; Rossant J; Wu MY; Piscione TD; Nagy A; Gossler A; Hicks GG; Hui CC; Henkelman RM; Yu LX; Sled JG; Gridley T; Egan SE. 2010. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol 298(1):L45-56. [PubMed: 19897741] [MGI Ref ID J:155893]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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