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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J (Changed: 23-NOV-05 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F? +F11pN1
Generation DefinitionsDonating Investigator Dr. Andras Nagy, Mount Sinai Hospital Description
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the Cre-transgenic line and the doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.Of note, mutant mice are also available on a C57BL/6J genetic background (see Stock No. 005670).
Development
A targeting vector containing a loxP-flanked phosphoglycerate kinase-neomycin resistance gene-polyadenylation stop sequence (PGK-neo-pA) fragment and a downstream reverse tetracycline-controlled transactivator gene-internal ribosomal entry site-enhanced green fluorescent protein gene-polyadenylation stop sequence (rtTA-IRES-EGFP-pA) fusion protein was inserted between exon 1 and 2 of the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with ICR morulae and the resulting chimeric males were backcrossed for germ-line transmission to ICR females. Heterozygous offspring were intercrossed to produce wild type, heterozygotes, and homozygotes.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andras Nagy | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(rtTA)Nagy; Gt(ROSA)26Sortm1Nagy; R26rtTA; ROSA26-rtTA; ROSA26-rtTA-IRES-EGFP; Rosa26fs-rtTA; rtTAflox; | ||
| Mutation Made By | Gusztav Belteki and Jody Haigh, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. [MGI Ref ID J:80963] [MGI Ref ID J:99607] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy, Separated PCR
ROSA26 Generic GFP, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Belteki G; Haigh J; Kabacs N; Haigh K; Sison K; Costantini F; Whitsett J; Quaggin SE; Nagy A. 2005. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res 33(5):e51. [PubMed: 15784609] [MGI Ref ID J:99607]
Additional References
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy relatedBonilla-Claudio M; Wang J; Bai Y; Klysik E; Selever J; Martin JF. 2012. Bmp signaling regulates a dose-dependent transcriptional program to control facial skeletal development. Development 139(4):709-19. [PubMed: 22219353] [MGI Ref ID J:181229]
Collins MA; Bednar F; Zhang Y; Brisset JC; Galban S; Galban CJ; Rakshit S; Flannagan KS; Adsay NV; Pasca di Magliano M. 2012. Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice. J Clin Invest 122(2):639-53. [PubMed: 22232209] [MGI Ref ID J:184378]
Collins MA; Brisset JC; Zhang Y; Bednar F; Pierre J; Heist KA; Galban CJ; Galban S; di Magliano MP. 2012. Metastatic pancreatic cancer is dependent on oncogenic Kras in mice. PLoS One 7(12):e49707. [PubMed: 23226501] [MGI Ref ID J:195686]
Deng Y; Wang ZV; Tao C; Gao N; Holland WL; Ferdous A; Repa JJ; Liang G; Ye J; Lehrman MA; Hill JA; Horton JD; Scherer PE. 2013. The Xbp1s/GalE axis links ER stress to postprandial hepatic metabolism. J Clin Invest 123(1):455-68. [PubMed: 23257357] [MGI Ref ID J:194290]
Drogat B; Kalucka J; Gutierrez L; Hammad H; Goossens S; Farhang Ghahremani M; Bartunkova S; Haigh K; Deswarte K; Nyabi O; Naessens M; Ferrara N; Klingmuller U; Lambrecht BN; Nagy A; Philipsen S; Haigh JJ. 2010. Vegf regulates embryonic erythroid development through Gata1 modulation. Blood 116(12):2141-51. [PubMed: 20554972] [MGI Ref ID J:164506]
El Andaloussi A; Graves S; Meng F; Mandal M; Mashayekhi M; Aifantis I. 2006. Hedgehog signaling controls thymocyte progenitor homeostasis and differentiation in the thymus. Nat Immunol 7(4):418-26. [PubMed: 16518394] [MGI Ref ID J:112555]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Grachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145] [MGI Ref ID J:173930]
Gupte VV; Ramasamy SK; Reddy R; Lee J; Weinreb PH; Violette SM; Guenther A; Warburton D; Driscoll B; Minoo P; Bellusci S. 2009. Overexpression of fibroblast growth factor-10 during both inflammatory and fibrotic phases attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Respir Crit Care Med 180(5):424-36. [PubMed: 19498056] [MGI Ref ID J:167964]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2010. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem :. [PubMed: 21136430] [MGI Ref ID J:171769]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2011. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem 112(2):653-65. [PubMed: 21268087] [MGI Ref ID J:168061]
Jeansson M; Gawlik A; Anderson G; Li C; Kerjaschki D; Henkelman M; Quaggin SE. 2011. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury. J Clin Invest 121(6):2278-89. [PubMed: 21606590] [MGI Ref ID J:174022]
Kelly MC; Chang Q; Pan A; Lin X; Chen P. 2012. Atoh1 directs the formation of sensory mosaics and induces cell proliferation in the postnatal Mammalian cochlea in vivo. J Neurosci 32(19):6699-710. [PubMed: 22573692] [MGI Ref ID J:184847]
Kim ST; Adair-Kirk TL; Senior RM; Miner JH. 2012. Functional consequences of cell type-restricted expression of laminin alpha5 in mouse placental labyrinth and kidney glomerular capillaries. PLoS One 7(7):e41348. [PubMed: 22911783] [MGI Ref ID J:189883]
Kocabas F; Zheng J; Thet S; Copeland NG; Jenkins NA; DeBerardinis RJ; Zhang C; Sadek HA. 2012. Meis1 regulates the metabolic phenotype and oxidant defense of hematopoietic stem cells. Blood 120(25):4963-72. [PubMed: 22995899] [MGI Ref ID J:192126]
Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91. [PubMed: 21672919] [MGI Ref ID J:174960]
Lin HY; Yang LT. 2013. Differential response of epithelial stem cell populations in hair follicles to TGF-beta signaling. Dev Biol 373(2):394-406. [PubMed: 23103542] [MGI Ref ID J:192167]
Maes C; Goossens S; Bartunkova S; Drogat B; Coenegrachts L; Stockmans I; Moermans K; Nyabi O; Haigh K; Naessens M; Haenebalcke L; Tuckermann JP; Tjwa M; Carmeliet P; Mandic V; David JP; Behrens A; Nagy A; Carmeliet G; Haigh JJ. 2010. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J 29(2):424-41. [PubMed: 20010698] [MGI Ref ID J:156474]
Moriyama M; Fukuhara T; Britschgi M; He Y; Narasimhan R; Villeda S; Molina H; Huber BT; Holers M; Wyss-Coray T. 2011. Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis. J Neurosci 31(11):3981-3989. [PubMed: 21411641] [MGI Ref ID J:170456]
Noah TK; Kazanjian A; Whitsett J; Shroyer NF. 2010. SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Exp Cell Res 316(3):452-65. [PubMed: 19786015] [MGI Ref ID J:156638]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Parsa S; Kuremoto K; Seidel K; Tabatabai R; Mackenzie B; Yamaza T; Akiyama K; Branch J; Koh CJ; Al Alam D; Klein OD; Bellusci S. 2010. Signaling by FGFR2b controls the regenerative capacity of adult mouse incisors. Development 137(22):3743-52. [PubMed: 20978072] [MGI Ref ID J:167068]
Parsa S; Ramasamy SK; De Langhe S; Gupte VV; Haigh JJ; Medina D; Bellusci S. 2008. Terminal end bud maintenance in mammary gland is dependent upon FGFR2b signaling. Dev Biol 317(1):121-31. [PubMed: 18381212] [MGI Ref ID J:136174]
Pi M; Chen L; Huang M; Luo Q; Quarles LD. 2008. Parathyroid-specific interaction of the calcium-sensing receptor and G alpha q. Kidney Int 74(12):1548-56. [PubMed: 18813283] [MGI Ref ID J:162715]
Sala FG; Del Moral PM; Tiozzo C; Alam DA; Warburton D; Grikscheit T; Veltmaat JM; Bellusci S. 2011. FGF10 controls the patterning of the tracheal cartilage rings via Shh. Development 138(2):273-82. [PubMed: 21148187] [MGI Ref ID J:167739]
Schlegelmilch K; Mohseni M; Kirak O; Pruszak J; Rodriguez JR; Zhou D; Kreger BT; Vasioukhin V; Avruch J; Brummelkamp TR; Camargo FD. 2011. Yap1 acts downstream of alpha-catenin to control epidermal proliferation. Cell 144(5):782-95. [PubMed: 21376238] [MGI Ref ID J:171057]
Sharir A; Stern T; Rot C; Shahar R; Zelzer E. 2011. Muscle force regulates bone shaping for optimal load-bearing capacity during embryogenesis. Development 138(15):3247-59. [PubMed: 21750035] [MGI Ref ID J:180904]
Sison K; Eremina V; Baelde H; Min W; Hirashima M; Fantus IG; Quaggin SE. 2010. Glomerular structure and function require paracrine, not autocrine, VEGF-VEGFR-2 signaling. J Am Soc Nephrol 21(10):1691-701. [PubMed: 20688931] [MGI Ref ID J:185910]
Speer AL; Al Alam D; Sala FG; Ford HR; Bellusci S; Grikscheit TC. 2012. Fibroblast growth factor 10-fibroblast growth factor receptor 2b mediated signaling is not required for adult glandular stomach homeostasis. PLoS One 7(11):e49127. [PubMed: 23133671] [MGI Ref ID J:195033]
Srinivas S; Watanabe T; Lin CS; William CM; Tanabe Y; Jessell TM; Costantini F. 2001. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1(1):4. [PubMed: 11299042] [MGI Ref ID J:80963]
Sung HK; Doh KO; Son JE; Park JG; Bae Y; Choi S; Nelson SM; Cowling R; Nagy K; Michael IP; Koh GY; Adamson SL; Pawson T; Nagy A. 2013. Adipose vascular endothelial growth factor regulates metabolic homeostasis through angiogenesis. Cell Metab 17(1):61-72. [PubMed: 23312284] [MGI Ref ID J:195071]
Takase HM; Itoh T; Ino S; Wang T; Koji T; Akira S; Takikawa Y; Miyajima A. 2013. FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration. Genes Dev 27(2):169-81. [PubMed: 23322300] [MGI Ref ID J:193313]
Tang S; Snider P; Firulli AB; Conway SJ. 2010. Trigenic neural crest-restricted Smad7 over-expression results in congenital craniofacial and cardiovascular defects. Dev Biol 344(1):233-47. [PubMed: 20457144] [MGI Ref ID J:163879]
Vega-Hernandez M; Kovacs A; De Langhe S; Ornitz DM. 2011. FGF10/FGFR2b signaling is essential for cardiac fibroblast development and growth of the myocardium. Development 138(15):3331-40. [PubMed: 21750042] [MGI Ref ID J:175538]
Volckaert T; Dill E; Campbell A; Tiozzo C; Majka S; Bellusci S; De Langhe SP. 2011. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J Clin Invest 121(11):4409-19. [PubMed: 21985786] [MGI Ref ID J:178444]
Wang J; Greene SB; Bonilla-Claudio M; Tao Y; Zhang J; Bai Y; Huang Z; Black BL; Wang F; Martin JF. 2010. Bmp signaling regulates myocardial differentiation from cardiac progenitors through a MicroRNA-mediated mechanism. Dev Cell 19(6):903-12. [PubMed: 21145505] [MGI Ref ID J:169047]
Wang Y; Jarad G; Tripathi P; Pan M; Cunningham J; Martin DR; Liapis H; Miner JH; Chen F. 2010. Activation of NFAT signaling in podocytes causes glomerulosclerosis. J Am Soc Nephrol 21(10):1657-66. [PubMed: 20651158] [MGI Ref ID J:185917]
Wehn AK; Chapman DL. 2010. Tbx18 and Tbx15 null-like phenotypes in mouse embryos expressing Tbx6 in somitic and lateral plate mesoderm. Dev Biol 347(2):404-13. [PubMed: 20832395] [MGI Ref ID J:166775]
Wehn AK; Gallo PH; Chapman DL. 2009. Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element. Genesis 47(5):309-313. [PubMed: 19298012] [MGI Ref ID J:148512]
Xu K; Nieuwenhuis E; Cohen BL; Wang W; Canty AJ; Danska JS; Coultas L; Rossant J; Wu MY; Piscione TD; Nagy A; Gossler A; Hicks GG; Hui CC; Henkelman RM; Yu LX; Sled JG; Gridley T; Egan SE. 2010. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol 298(1):L45-56. [PubMed: 19897741] [MGI Ref ID J:155893]
Ying H; Kimmelman AC; Lyssiotis CA; Hua S; Chu GC; Fletcher-Sananikone E; Locasale JW; Son J; Zhang H; Coloff JL; Yan H; Wang W; Chen S; Viale A; Zheng H; Paik JH; Lim C; Guimaraes AR; Martin ES; Chang J; Hezel AF; Perry SR; Hu J; Gan B; Xiao Y; Asara JM; Weissleder R; Wang YA; Chin L; Cantley LC; DePinho RA. 2012. Oncogenic Kras maintains pancreatic tumors through regulation of anabolic glucose metabolism. Cell 149(3):656-70. [PubMed: 22541435] [MGI Ref ID J:186194]
von Gise A; Lin Z; Schlegelmilch K; Honor LB; Pan GM; Buck JN; Ma Q; Ishiwata T; Zhou B; Camargo FD; Pu WT. 2012. YAP1, the nuclear target of Hippo signaling, stimulates heart growth through cardiomyocyte proliferation but not hypertrophy. Proc Natl Acad Sci U S A 109(7):2394-9. [PubMed: 22308401] [MGI Ref ID J:182615]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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