Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   20-JUN-06 Species laboratory mouse Generation N7pF14N1F4 (27-NOV-11) Generation Definitions   Donating Investigator Christopher A. Gabel,   Pfizer Pharmaceuticals

Description
Mice that are homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No full length gene product (mRNA or protein) is detected in cultured bone marrow mast cells or peritoneal macrophages. Samples of whole blood, as well as peritoneal macrophages, derived from mutant mice fail to produce extracellular interleukin 1 beta in response to lipopolysaccharide (LPS) and ATP treatment. Similarly, peritoneal lavage fluids from mutant animals that have been primed with LPS and subsequently challenged with ATP, are deficient in mature interleukin 1 beta, and at later time points, exhibit attenuated interleukin 6 levels when compared to fluids from similarly treated wildtype mice. Peripheral blood monocytes and leukocytes fail to change shape/volume and shed L-selectin in response to ATP. Mutant mice exhibit reduced induction and severity of monoclonal anti-collagen-induced arthritis. Mutant mice also display significant reduction in femoral periosteal circumference, reduced periosteal bone formation, and a reduction in total and cortical bone content. Increased resorption in tibial trabecular bone tissue is observed.

Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt the carboxyl-terminal coding region of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator stated that the resulting chimeric animals were backcrossed to C57BL/6 mice (see SNP note below) for 7 generations.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

	Control
	See control note:	C57BL/6J mice carry a naturally occurring point mutation in P2rx7 resulting in a proline to leucine amino acid substitution at residue 451 (P451L).
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   P2rx7^tm1Gab allele

015809

NOD.129P2(B6)-P2rx7tm1Gab/DvsJ

View Strains carrying   P2rx7^tm1Gab     (1 strain)

Strains carrying other alleles of P2rx7

000665	C57BL/10J
000664	C57BL/6J
000670	DBA/1J
000671	DBA/2J

View Strains carrying other alleles of P2rx7     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

P2rx7^tm1Gab/P2rx7^tm1Gab

        B6.129P2-P2rx7^tm1Gab/J

• immune system phenotype
• abnormal mast cell physiology
  • bone marrow-derived mast cells fail to exhibit ATP-stimulated citrullination unlike wild-type cells   (MGI Ref ID J:190651)
  • however, cells exhibit normal citrullination in response to PMA and ionomycin stimulation   (MGI Ref ID J:190651)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

P2rx7^tm1Gab/P2rx7^tm1Gab

        either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * DBA/2)

• cellular phenotype
• abnormal cell death
  • lysis triggered by extracellular ATP does not occur   (MGI Ref ID J:89513)
• growth/size phenotype
• decreased body weight
  • although body weight was normal in males at birth, it was about 16% less than normal at 9 months of age   (MGI Ref ID J:84145)
• immune system phenotype
• abnormal interleukin-6 secretion
  • IL-6 release as a result of ATP challenge as opposed to PBS challenge increases similar to wild-type mice after 30 minutes   (MGI Ref ID J:66835)
  • 120 minutes after ATP challenge, IL-6 release drops as compared to continued increase in wild-type mice, although still greater than after a PBS challenge   (MGI Ref ID J:66835)
• decreased interleukin-1 beta secretion
  • although pro-Il1beta is produced by peritoneal machrophages, no mature IL-1beta is produced or released as a result of ATP challenge   (MGI Ref ID J:66835)
• increased osteoclast cell number
  • increased number of osteoclasts in tibial trabecular bone   (MGI Ref ID J:84145)
• limbs/digits/tail phenotype
• decreased diameter of femur   (MGI Ref ID J:89513)
• decreased diameter of tibia   (MGI Ref ID J:89513)
• skeleton phenotype
• abnormal skeleton development
  • lower total bone content   (MGI Ref ID J:84145)
• abnormal skeleton physiology
  • reduced periosteal bone expansion in long bones   (MGI Ref ID J:84145)
• decreased diameter of long bones
  • diameters of femurs and tibia were reduced although lengths were normal   (MGI Ref ID J:84145)
• • decreased diameter of femur   (MGI Ref ID J:89513)
  • decreased diameter of tibia   (MGI Ref ID J:89513)
• increased osteoclast cell number
  • increased number of osteoclasts in tibial trabecular bone   (MGI Ref ID J:84145)
• hematopoietic system phenotype
• increased osteoclast cell number
  • increased number of osteoclasts in tibial trabecular bone   (MGI Ref ID J:84145)

P2rx7^tm1Gab/P2rx7^tm1Gab

        involves: 129P2/OlaHsd * C57BL/6

• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype
  • calcium signal propagation in cochlear cultures is normal   (MGI Ref ID J:178055)
  • sensitivity to extracellular calcium and ectonucleaotidase activity is normal   (MGI Ref ID J:178055)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Skeletal Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		P2rx7tm1Gab
Allele Name		targeted mutation 1, Christopher A Gabel
Allele Type		Targeted (knock-out)
Common Name(s)		P2X7 KO; P2X7-; P2X7R-; P2X7RDelta506-532; Pfizer P2X7-;
Mutation Made By		Patrick Gillespie,   Pfizer Pharmaceuticals
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14TG2a
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		P2rx7, purinergic receptor P2X, ligand-gated ion channel, 7
Chromosome		5
Gene Common Name(s)		AI467586; P2X(7); P2X7; P2X7 receptor; P2X7R; expressed sequence AI467586;
Molecular Note		Sequence encoding amino acids 506 through 532 was replaced by the insertion of a neomycin selection cassette. Transcript was undetected by Northern blot analysis of bone marrow mast cells isolated from homozygous mutant mice. Western blot analysis of homozygous mutant peritoneal macrophages showed an absence of normal and truncated protein. [MGI Ref ID J:66835]

Genotyping

Genotyping Information

Genotyping Protocols

P2rx7^tm1Gab, Melt Curve Analysis
P2rx7^tm1Gab, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Solle M; Labasi J; Perregaux DG; Stam E; Petrushova N; Koller BH; Griffiths RJ; Gabel CA. 2001. Altered cytokine production in mice lacking P2X(7) receptors. J Biol Chem 276(1):125-32. [PubMed: 11016935]  [MGI Ref ID J:66835]

Additional References

P2rx7^tm1Gab related

Allam R; Darisipudi MN; Rupanagudi KV; Lichtnekert J; Tschopp J; Anders HJ. 2011. Cutting Edge: Cyclic Polypeptide and Aminoglycoside Antibiotics Trigger IL-1{beta} Secretion by Activating the NLRP3 Inflammasome. J Immunol 186(5):2714-8. [PubMed: 21278344]  [MGI Ref ID J:169383]

Anselmi F; Hernandez VH; Crispino G; Seydel A; Ortolano S; Roper SD; Kessaris N; Richardson W; Rickheit G; Filippov MA; Monyer H; Mammano F. 2008. ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear. Proc Natl Acad Sci U S A 105(48):18770-5. [PubMed: 19047635]  [MGI Ref ID J:178055]

Arandjelovic S; McKenney KR; Leming SS; Mowen KA. 2012. ATP induces protein arginine deiminase 2-dependent citrullination in mast cells through the P2X7 purinergic receptor. J Immunol 189(8):4112-22. [PubMed: 22984079]  [MGI Ref ID J:190651]

Aswad F; Dennert G. 2006. P2X(7) receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes. Cell Immunol 243(1):58-65. [PubMed: 17286969]  [MGI Ref ID J:118682]

Aswad F; Kawamura H; Dennert G. 2005. High sensitivity of CD4+CD25+ regulatory T cells to extracellular metabolites nicotinamide adenine dinucleotide and ATP: a role for P2X7 receptors. J Immunol 175(5):3075-83. [PubMed: 16116196]  [MGI Ref ID J:113208]

Auger R; Motta I; Benihoud K; Ojcius DM; Kanellopoulos JM. 2005. A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation in P2X7 receptor-mediated thymocyte death. J Biol Chem 280(30):28142-51. [PubMed: 15937334]  [MGI Ref ID J:100823]

Boucher AA; Arnold JC; Hunt GE; Spiro A; Spencer J; Brown C; McGregor IS; Bennett MR; Kassiou M. 2011. Resilience and reduced c-Fos expression in P2X7 receptor knockout mice exposed to repeated forced swim test. Neuroscience 189:170-7. [PubMed: 21664437]  [MGI Ref ID J:175649]

Bulanova E; Budagian V; Orinska Z; Koch-Nolte F; Haag F; Bulfone-Paus S. 2009. ATP induces P2X7 receptor-independent cytokine and chemokine expression through P2X1 and P2X3 receptors in murine mast cells. J Leukoc Biol 85(4):692-702. [PubMed: 19164130]  [MGI Ref ID J:146897]

Byrne SN; Beaugie C; O'Sullivan C; Leighton S; Halliday GM. 2011. The Immune-Modulating Cytokine and Endogenous Alarmin Interleukin-33 Is Upregulated in Skin Exposed to Inflammatory UVB Radiation. Am J Pathol 179(1):211-22. [PubMed: 21703403]  [MGI Ref ID J:173685]

Cao L; Li L; Lin D; Zuo Z. 2012. Isoflurane induces learning impairment that is mediated by interleukin 1beta in rodents. PLoS One 7(12):e51431. [PubMed: 23251531]  [MGI Ref ID J:195665]

Chatterjee S; Rana R; Corbett J; Kadiiska MB; Goldstein J; Mason RP. 2012. P2X7 receptor-NADPH oxidase axis mediates protein radical formation and Kupffer cell activation in carbon tetrachloride-mediated steatohepatitis in obese mice. Free Radic Biol Med 52(9):1666-79. [PubMed: 22343416]  [MGI Ref ID J:183259]

Chen L; Brosnan CF. 2006. Exacerbation of experimental autoimmune encephalomyelitis in P2X7R-/- mice: evidence for loss of apoptotic activity in lymphocytes. J Immunol 176(5):3115-26. [PubMed: 16493071]  [MGI Ref ID J:129413]

Chen YG; Scheuplein F; Driver JP; Hewes AA; Reifsnyder PC; Leiter EH; Serreze DV. 2011. Testing the Role of P2X7 Receptors in the Development of Type 1 Diabetes in Nonobese Diabetic Mice. J Immunol :. [PubMed: 21357538]  [MGI Ref ID J:169661]

Cowley SC; Hamilton E; Frelinger JA; Su J; Forman J; Elkins KL. 2005. CD4-CD8- T cells control intracellular bacterial infections both in vitro and in vivo. J Exp Med 202(2):309-19. [PubMed: 16027239]  [MGI Ref ID J:100513]

Daniel C; Wennhold K; Kim HJ; von Boehmer H. 2010. Enhancement of antigen-specific Treg vaccination in vivo. Proc Natl Acad Sci U S A 107(37):16246-51. [PubMed: 20805478]  [MGI Ref ID J:164370]

Darville T; Welter-Stahl L; Cruz C; Sater AA; Andrews CW Jr; Ojcius DM. 2007. Effect of the purinergic receptor P2X7 on Chlamydia infection in cervical epithelial cells and vaginally infected mice. J Immunol 179(6):3707-14. [PubMed: 17785807]  [MGI Ref ID J:152047]

Delarasse C; Auger R; Gonnord P; Fontaine B; Kanellopoulos JM. 2011. The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein. J Biol Chem 286(4):2596-606. [PubMed: 21081501]  [MGI Ref ID J:168499]

Deplano S; Cook HT; Russell R; Franchi L; Schneiter S; Bhangal G; Unwin RJ; Pusey CD; Tam FW; Behmoaras J. 2013. P2X7 receptor-mediated Nlrp3-inflammasome activation is a genetic determinant of macrophage-dependent crescentic glomerulonephritis. J Leukoc Biol 93(1):127-34. [PubMed: 23089744]  [MGI Ref ID J:193771]

Diaz-Hernandez M; Diez-Zaera M; Sanchez-Nogueiro J; Gomez-Villafuertes R; Canals JM; Alberch J; Miras-Portugal MT; Lucas JJ. 2009. Altered P2X7-receptor level and function in mouse models of Huntington's disease and therapeutic efficacy of antagonist administration. FASEB J 23(6):1893-906. [PubMed: 19171786]  [MGI Ref ID J:150552]

Dorhoi A; Nouailles G; Jorg S; Hagens K; Heinemann E; Pradl L; Oberbeck-Muller D; Duque-Correa MA; Reece ST; Ruland J; Brosch R; Tschopp J; Gross O; Kaufmann SH. 2012. Activation of the NLRP3 inflammasome by Mycobacterium tuberculosis is uncoupled from susceptibility to active tuberculosis. Eur J Immunol 42(2):374-84. [PubMed: 22101787]  [MGI Ref ID J:179821]

Dostert C; Guarda G; Romero JF; Menu P; Gross O; Tardivel A; Suva ML; Stehle JC; Kopf M; Stamenkovic I; Corradin G; Tschopp J. 2009. Malarial hemozoin is a Nalp3 inflammasome activating danger signal. PLoS One 4(8):e6510. [PubMed: 19652710]  [MGI Ref ID J:152483]

Eltom S; Stevenson CS; Rastrick J; Dale N; Raemdonck K; Wong S; Catley MC; Belvisi MG; Birrell MA. 2011. P2X7 receptor and caspase 1 activation are central to airway inflammation observed after exposure to tobacco smoke. PLoS One 6(9):e24097. [PubMed: 21915284]  [MGI Ref ID J:177698]

Franke H; Klimke K; Brinckmann U; Grosche J; Francke M; Sperlagh B; Reichenbach A; Liebert UG; Illes P. 2005. P2X(7) receptor-mRNA and -protein in the mouse retina; changes during retinal degeneration in BALBCrds mice. Neurochem Int 47(4):235-42. [PubMed: 15964665]  [MGI Ref ID J:103892]

Frascoli M; Marcandalli J; Schenk U; Grassi F. 2012. Purinergic P2X7 receptor drives T cell lineage choice and shapes peripheral gammadelta cells. J Immunol 189(1):174-80. [PubMed: 22649196]  [MGI Ref ID J:188945]

Furlan-Freguia C; Marchese P; Gruber A; Ruggeri ZM; Ruf W. 2011. P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in mice. J Clin Invest 121(7):2932-44. [PubMed: 21670495]  [MGI Ref ID J:175647]

Garcia-Marcos M; Fontanils U; Aguirre A; Pochet S; Dehaye JP; Marino A. 2005. Role of sodium in mitochondrial membrane depolarization induced by P2X7 receptor activation in submandibular glands. FEBS Lett 579(24):5407-13. [PubMed: 16198349]  [MGI Ref ID J:102387]

Ghiringhelli F; Apetoh L; Tesniere A; Aymeric L; Ma Y; Ortiz C; Vermaelen K; Panaretakis T; Mignot G; Ullrich E; Perfettini JL; Schlemmer F; Tasdemir E; Uhl M; Genin P; Civas A; Ryffel B; Kanellopoulos J; Tschopp J; Andre F; Lidereau R; McLaughlin NM; Haynes NM; Smyth MJ; Kroemer G; Zitvogel L. 2009. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors. Nat Med 15(10):1170-8. [PubMed: 19767732]  [MGI Ref ID J:154302]

Glas R; Sauter NS; Schulthess FT; Shu L; Oberholzer J; Maedler K. 2009. Purinergic P2X7 receptors regulate secretion of interleukin-1 receptor antagonist and beta cell function and survival. Diabetologia 52(8):1579-88. [PubMed: 19396427]  [MGI Ref ID J:151265]

Goncalves RG; Gabrich L; Rosario A Jr; Takiya CM; Ferreira ML; Chiarini LB; Persechini PM; Coutinho-Silva R; Leite M Jr. 2006. The role of purinergic P2X7 receptors in the inflammation and fibrosis of unilateral ureteral obstruction in mice. Kidney Int 70(9):1599-606. [PubMed: 16969386]  [MGI Ref ID J:136485]

Gu BJ; Saunders BM; Petrou S; Wiley JS. 2011. P2X(7) is a scavenger receptor for apoptotic cells in the absence of its ligand, extracellular ATP. J Immunol 187(5):2365-75. [PubMed: 21821797]  [MGI Ref ID J:179265]

Haanes KA; Schwab A; Novak I. 2012. The P2X7 receptor supports both life and death in fibrogenic pancreatic stellate cells. PLoS One 7(12):e51164. [PubMed: 23284663]  [MGI Ref ID J:195637]

Habbas S; Ango F; Daniel H; Galante M. 2011. Purinergic signaling in the cerebellum: Bergmann glial cells express functional ionotropic P2X(7) receptors. Glia :. [PubMed: 21830236]  [MGI Ref ID J:176597]

He X; Mekasha S; Mavrogiorgos N; Fitzgerald KA; Lien E; Ingalls RR. 2010. Inflammation and fibrosis during Chlamydia pneumoniae infection is regulated by IL-1 and the NLRP3/ASC inflammasome. J Immunol 184(10):5743-54. [PubMed: 20393140]  [MGI Ref ID J:161000]

Heiss K; Janner N; Mahnss B; Schumacher V; Koch-Nolte F; Haag F; Mittrucker HW. 2008. High sensitivity of intestinal CD8+ T cells to nucleotides indicates P2X7 as a regulator for intestinal T cell responses. J Immunol 181(6):3861-9. [PubMed: 18768840]  [MGI Ref ID J:139109]

Hoque R; Sohail MA; Salhanick S; Malik AF; Ghani A; Robson SC; Mehal WZ. 2012. P2X7 receptor-mediated purinergic signaling promotes liver injury in acetaminophen hepatotoxicity in mice. Am J Physiol Gastrointest Liver Physiol 302(10):G1171-9. [PubMed: 22383490]  [MGI Ref ID J:190176]

Hubert S; Rissiek B; Klages K; Huehn J; Sparwasser T; Haag F; Koch-Nolte F; Boyer O; Seman M; Adriouch S. 2010. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med 207(12):2561-8. [PubMed: 20975043]  [MGI Ref ID J:176876]

Iyer SS; Pulskens WP; Sadler JJ; Butter LM; Teske GJ; Ulland TK; Eisenbarth SC; Florquin S; Flavell RA; Leemans JC; Sutterwala FS. 2009. Necrotic cells trigger a sterile inflammatory response through the Nlrp3 inflammasome. Proc Natl Acad Sci U S A 106(48):20388-93. [PubMed: 19918053]  [MGI Ref ID J:155576]

Jabs R; Matthias K; Grote A; Grauer M; Seifert G; Steinhauser C. 2007. Lack of P2X receptor mediated currents in astrocytes and GluR type glial cells of the hippocampal CA1 region. Glia 55(16):1648-55. [PubMed: 17849469]  [MGI Ref ID J:156297]

Ji X; Naito Y; Weng H; Endo K; Ma X; Iwai N. 2012. P2X7 deficiency attenuates hypertension and renal injury in deoxycorticosterone acetate-salt hypertension. Am J Physiol Renal Physiol 303(8):F1207-15. [PubMed: 22859404]  [MGI Ref ID J:188617]

Jin C; Frayssinet P; Pelker R; Cwirka D; Hu B; Vignery A; Eisenbarth SC; Flavell RA. 2011. NLRP3 inflammasome plays a critical role in the pathogenesis of hydroxyapatite-associated arthropathy. Proc Natl Acad Sci U S A 108(36):14867-72. [PubMed: 21856950]  [MGI Ref ID J:175225]

Jones CL; Weiss DS. 2011. TLR2 signaling contributes to rapid inflammasome activation during F. novicida infection. PLoS One 6(6):e20609. [PubMed: 21698237]  [MGI Ref ID J:174784]

Kahlenberg JM; Carmona-Rivera C; Smith CK; Kaplan MJ. 2013. Neutrophil Extracellular Trap-Associated Protein Activation of the NLRP3 Inflammasome Is Enhanced in Lupus Macrophages. J Immunol 190(3):1217-26. [PubMed: 23267025]  [MGI Ref ID J:192601]

Kawamura H; Aswad F; Minagawa M; Govindarajan S; Dennert G. 2006. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol 176(4):2152-60. [PubMed: 16455971]  [MGI Ref ID J:129123]

Kawamura H; Kawamura T; Kanda Y; Kobayashi T; Abo T. 2012. Extracellular ATP-stimulated macrophages produce macrophage inflammatory protein-2 which is important for neutrophil migration. Immunology 136(4):448-58. [PubMed: 22564028]  [MGI Ref ID J:186262]

Ke HZ; Qi H; Weidema AF; Zhang Q; Panupinthu N; Crawford DT; Grasser WA; Paralkar VM; Li M; Audoly LP; Gabel CA; Jee WS; Dixon SJ; Sims SM; Thompson DD. 2003. Deletion of the P2X7 nucleotide receptor reveals its regulatory roles in bone formation and resorption. Mol Endocrinol 17(7):1356-67. [PubMed: 12677010]  [MGI Ref ID J:84145]

Kim JE; Kang TC. 2011. The P2X7 receptor-pannexin-1 complex decreases muscarinic acetylcholine receptor-mediated seizure susceptibility in mice. J Clin Invest 121(5):2037-47. [PubMed: 21505260]  [MGI Ref ID J:173942]

Kimbler DE; Shields J; Yanasak N; Vender JR; Dhandapani KM. 2012. Activation of P2X7 promotes cerebral edema and neurological injury after traumatic brain injury in mice. PLoS One 7(7):e41229. [PubMed: 22815977]  [MGI Ref ID J:189601]

Kouzaki H; Iijima K; Kobayashi T; O'Grady SM; Kita H. 2011. The Danger Signal, Extracellular ATP, Is a Sensor for an Airborne Allergen and Triggers IL-33 Release and Innate Th2-Type Responses. J Immunol 186(7):4375-87. [PubMed: 21357533]  [MGI Ref ID J:170695]

Kovarova M; Hesker PR; Jania L; Nguyen M; Snouwaert JN; Xiang Z; Lommatzsch SE; Huang MT; Ting JP; Koller BH. 2012. NLRP1-dependent pyroptosis leads to acute lung injury and morbidity in mice. J Immunol 189(4):2006-16. [PubMed: 22753929]  [MGI Ref ID J:189777]

Kuehnel MP; Reiss M; Anand PK; Treede I; Holzer D; Hoffmann E; Klapperstueck M; Steinberg TH; Markwardt F; Griffiths G. 2009. Sphingosine-1-phosphate receptors stimulate macrophage plasma-membrane actin assembly via ADP release, ATP synthesis and P2X7R activation. J Cell Sci 122(Pt 4):505-12. [PubMed: 19174470]  [MGI Ref ID J:146293]

Kuehnel MP; Rybin V; Anand PK; Anes E; Griffiths G. 2009. Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen. J Cell Sci 122(Pt 4):499-504. [PubMed: 19174471]  [MGI Ref ID J:146292]

Kurashima Y; Amiya T; Nochi T; Fujisawa K; Haraguchi T; Iba H; Tsutsui H; Sato S; Nakajima S; Iijima H; Kubo M; Kunisawa J; Kiyono H. 2012. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors. Nat Commun 3:1034. [PubMed: 22948816]  [MGI Ref ID J:195470]

Labasi JM; Petrushova N; Donovan C; McCurdy S; Lira P; Payette MM; Brissette W; Wicks JR; Audoly L; Gabel CA. 2002. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J Immunol 168(12):6436-45. [PubMed: 12055263]  [MGI Ref ID J:111397]

Labrousse VF; Costes L; Aubert A; Darnaudery M; Ferreira G; Amedee T; Laye S. 2009. Impaired interleukin-1beta and c-Fos expression in the hippocampus is associated with a spatial memory deficit in P2X(7) receptor-deficient mice. PLoS One 4(6):e6006. [PubMed: 19547756]  [MGI Ref ID J:150192]

Le Gall SM; Legrand J; Benbijja M; Safya H; Benihoud K; Kanellopoulos JM; Bobe P. 2012. Loss of P2X7 receptor plasma membrane expression and function in pathogenic B220+ double-negative T lymphocytes of autoimmune MRL/lpr mice. PLoS One 7(12):e52161. [PubMed: 23284917]  [MGI Ref ID J:195619]

Le Stunff H; Auger R; Kanellopoulos J; Raymond MN. 2004. The Pro-451 to Leu polymorphism within the C-terminal tail of P2X7 receptor impairs cell death but not phospholipase D activation in murine thymocytes. J Biol Chem 279(17):16918-26. [PubMed: 14761980]  [MGI Ref ID J:89513]

Lee BH; Hwang DM; Palaniyar N; Grinstein S; Philpott DJ; Hu J. 2012. Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection. PLoS One 7(4):e35812. [PubMed: 22558229]  [MGI Ref ID J:187281]

Lee GS; Subramanian N; Kim AI; Aksentijevich I; Goldbach-Mansky R; Sacks DB; Germain RN; Kastner DL; Chae JJ. 2012. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature 492(7427):123-7. [PubMed: 23143333]  [MGI Ref ID J:192821]

Li J; Liu D; Ke HZ; Duncan RL; Turner CH. 2005. The P2X7 nucleotide receptor mediates skeletal mechanotransduction. J Biol Chem 280(52):42952-9. [PubMed: 16269410]  [MGI Ref ID J:105908]

Lu R; Pan H; Shively JE. 2012. CEACAM1 negatively regulates IL-1beta production in LPS activated neutrophils by recruiting SHP-1 to a SYK-TLR4-CEACAM1 complex. PLoS Pathog 8(4):e1002597. [PubMed: 22496641]  [MGI Ref ID J:195394]

Mankus C; Chi C; Rich C; Ren R; Trinkaus-Randall V. 2012. The P2X(7) receptor regulates proteoglycan expression in the corneal stroma. Mol Vis 18:128-38. [PubMed: 22275804]  [MGI Ref ID J:191496]

Marin-Garcia P; Sanchez-Nogueiro J; Gomez-Villafuertes R; Leon D; Miras-Portugal MT. 2008. Synaptic terminals from mice midbrain exhibit functional P2X(7) receptor. Neuroscience 151(2):361-73. [PubMed: 18082965]  [MGI Ref ID J:130842]

Masin M; Young C; Lim K; Barnes SJ; Xu XJ; Marschall V; Brutkowski W; Mooney ER; Gorecki DC; Murrell-Lagnado R. 2012. Expression, assembly and function of novel C-terminal truncated variants of the mouse P2X7 receptor: re-evaluation of P2X7 knockouts. Br J Pharmacol 165(4):978-93. [PubMed: 21838754]  [MGI Ref ID J:194518]

Mayo C; Ren R; Rich C; Stepp MA; Trinkaus-Randall V. 2008. Regulation by P2X7: epithelial migration and stromal organization in the cornea. Invest Ophthalmol Vis Sci 49(10):4384-91. [PubMed: 18502993]  [MGI Ref ID J:141813]

Meuser-Batista M; Correa JR; Carvalho VF; de Carvalho Britto CF; da Cruz Moreira O; Batista MM; Soares MJ; Filho FA; E Silva PM; Lannes-Vieira J; Silva RC; Henriques-Pons A. 2011. Mast Cell Function and Death in Trypanosoma cruzi Infection. Am J Pathol 179(4):1894-904. [PubMed: 21819958]  [MGI Ref ID J:176304]

Mishra A; Chintagari NR; Guo Y; Weng T; Su L; Liu L. 2011. Purinergic P2X7 receptor regulates lung surfactant secretion in a paracrine manner. J Cell Sci 124(Pt 4):657-68. [PubMed: 21266468]  [MGI Ref ID J:182878]

Miyazaki T; Iwasawa M; Nakashima T; Mori S; Shigemoto K; Nakamura H; Katagiri H; Takayanagi H; Tanaka S. 2012. Intracellular and extracellular ATP coordinately regulate the inverse correlation between osteoclast survival and bone resorption. J Biol Chem 287(45):37808-23. [PubMed: 22988253]  [MGI Ref ID J:192471]

Munoz-Planillo R; Franchi L; Miller LS; Nunez G. 2009. A critical role for hemolysins and bacterial lipoproteins in Staphylococcus aureus-induced activation of the Nlrp3 inflammasome. J Immunol 183(6):3942-8. [PubMed: 19717510]  [MGI Ref ID J:152294]

Myers AJ; Eilertson B; Fulton SA; Flynn JL; Canaday DH. 2005. The purinergic P2X7 receptor is not required for control of pulmonary Mycobacterium tuberculosis infection. Infect Immun 73(5):3192-5. [PubMed: 15845532]  [MGI Ref ID J:97621]

Niessen F; Schaffner F; Furlan-Freguia C; Pawlinski R; Bhattacharjee G; Chun J; Derian CK; Andrade-Gordon P; Rosen H; Ruf W. 2008. Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature 452(7187):654-8. [PubMed: 18305483]  [MGI Ref ID J:133917]

Norton JT; Hayashi T; Crain B; Cho JS; Miller LS; Corr M; Carson DA. 2012. Cutting edge: nitrogen bisphosphonate-induced inflammation is dependent upon mast cells and IL-1. J Immunol 188(7):2977-80. [PubMed: 22387558]  [MGI Ref ID J:183090]

Notomi S; Hisatomi T; Kanemaru T; Takeda A; Ikeda Y; Enaida H; Kroemer G; Ishibashi T. 2011. Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am J Pathol 179(6):2798-809. [PubMed: 21983632]  [MGI Ref ID J:180087]

Novak I; Jans IM; Wohlfahrt L. 2010. Effect of P2X(7) receptor knockout on exocrine secretion of pancreas, salivary glands and lacrimal glands. J Physiol 588(Pt 18):3615-27. [PubMed: 20643770]  [MGI Ref ID J:176756]

Oliveira JF; Riedel T; Leichsenring A; Heine C; Franke H; Krugel U; Norenberg W; Illes P. 2011. Rodent cortical astroglia express in situ functional P2X7 receptors sensing pathologically high ATP concentrations. Cereb Cortex 21(4):806-20. [PubMed: 20739479]  [MGI Ref ID J:181824]

Panupinthu N; Rogers JT; Zhao L; Solano-Flores LP; Possmayer F; Sims SM; Dixon SJ. 2008. P2X7 receptors on osteoblasts couple to production of lysophosphatidic acid: a signaling axis promoting osteogenesis. J Cell Biol 181(5):859-71. [PubMed: 18519738]  [MGI Ref ID J:137037]

Panupinthu N; Zhao L; Possmayer F; Ke HZ; Sims SM; Dixon SJ. 2007. P2X7 nucleotide receptors mediate blebbing in osteoblasts through a pathway involving lysophosphatidic acid. J Biol Chem 282(5):3403-12. [PubMed: 17135244]  [MGI Ref ID J:120291]

Papp L; Vizi ES; Sperlagh B. 2004. Lack of ATP-evoked GABA and glutamate release in the hippocampus of P2X7 receptor-/- mice. Neuroreport 15(15):2387-91. [PubMed: 15640761]  [MGI Ref ID J:103703]

Qu Y; Franchi L; Nunez G; Dubyak GR. 2007. Nonclassical IL-1 beta secretion stimulated by P2X7 receptors is dependent on inflammasome activation and correlated with exosome release in murine macrophages. J Immunol 179(3):1913-25. [PubMed: 17641058]  [MGI Ref ID J:149937]

Ritter M; Gross O; Kays S; Ruland J; Nimmerjahn F; Saijo S; Tschopp J; Layland LE; Prazeres da Costa C. 2010. Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A 107(47):20459-64. [PubMed: 21059925]  [MGI Ref ID J:166590]

Sanchez-Nogueiro J; Marin-Garcia P; Miras-Portugal MT. 2005. Characterization of a functional P2X(7)-like receptor in cerebellar granule neurons from P2X(7) knockout mice. FEBS Lett 579(17):3783-8. [PubMed: 15978588]  [MGI Ref ID J:99778]

Sander LE; Davis MJ; Boekschoten MV; Amsen D; Dascher CC; Ryffel B; Swanson JA; Muller M; Blander JM. 2011. Detection of prokaryotic mRNA signifies microbial viability and promotes immunity. Nature 474(7351):385-9. [PubMed: 21602824]  [MGI Ref ID J:172745]

Sim JA; Young MT; Sung HY; North RA; Surprenant A. 2004. Reanalysis of P2X7 receptor expression in rodent brain. J Neurosci 24(28):6307-14. [PubMed: 15254086]  [MGI Ref ID J:97270]

Taylor SR; Gonzalez-Begne M; Dewhurst S; Chimini G; Higgins CF; Melvin JE; Elliott JI. 2008. Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death. J Immunol 180(1):300-8. [PubMed: 18097031]  [MGI Ref ID J:130933]

Taylor SR; Gonzalez-Begne M; Sojka DK; Richardson JC; Sheardown SA; Harrison SM; Pusey CD; Tam FW; Elliott JI. 2009. Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses. J Leukoc Biol 85(6):978-86. [PubMed: 19276178]  [MGI Ref ID J:149750]

Toma C; Higa N; Koizumi Y; Nakasone N; Ogura Y; McCoy AJ; Franchi L; Uematsu S; Sagara J; Taniguchi S; Tsutsui H; Akira S; Tschopp J; Nunez G; Suzuki T. 2010. Pathogenic Vibrio activate NLRP3 inflammasome via cytotoxins and TLR/nucleotide-binding oligomerization domain-mediated NF-kappaB signaling. J Immunol 184(9):5287-97. [PubMed: 20348425]  [MGI Ref ID J:160469]

Tourneur L; Mistou S; Schmitt A; Chiocchia G. 2008. Adenosine receptors control a new pathway of Fas-associated death domain protein expression regulation by secretion. J Biol Chem 283(26):17929-38. [PubMed: 18445587]  [MGI Ref ID J:138177]

Traves PG; Pimentel-Santillana M; Carrasquero LM; Perez-Sen R; Delicado EG; Luque A; Izquierdo M; Martin-Sanz P; Miras-Portugal MT; Bosca L. 2013. Selective Impairment of P2Y Signaling by Prostaglandin E2 in Macrophages: Implications for Ca2+-Dependent Responses. J Immunol 190(8):4226-35. [PubMed: 23479225]  [MGI Ref ID J:194901]

Vessey KA; Fletcher EL. 2012. Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse. PLoS One 7(1):e29990. [PubMed: 22253851]  [MGI Ref ID J:184314]

Weber FC; Esser PR; Muller T; Ganesan J; Pellegatti P; Simon MM; Zeiser R; Idzko M; Jakob T; Martin SF. 2010. Lack of the purinergic receptor P2X(7) results in resistance to contact hypersensitivity. J Exp Med 207(12):2609-19. [PubMed: 21059855]  [MGI Ref ID J:176873]

Wen H; Gris D; Lei Y; Jha S; Zhang L; Huang MT; Brickey WJ; Ting JP. 2011. Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nat Immunol 12(5):408-15. [PubMed: 21478880]  [MGI Ref ID J:172610]

Wilhelm K; Ganesan J; Muller T; Durr C; Grimm M; Beilhack A; Krempl CD; Sorichter S; Gerlach UV; Juttner E; Zerweck A; Gartner F; Pellegatti P; Di Virgilio F; Ferrari D; Kambham N; Fisch P; Finke J; Idzko M; Zeiser R. 2010. Graft-versus-host disease is enhanced by extracellular ATP activating P2X7R. Nat Med 16(12):1434-8. [PubMed: 21102458]  [MGI Ref ID J:167519]

Witting A; Chen L; Cudaback E; Straiker A; Walter L; Rickman B; Moller T; Brosnan C; Stella N. 2006. Experimental autoimmune encephalomyelitis disrupts endocannabinoid-mediated neuroprotection. Proc Natl Acad Sci U S A 103(16):6362-7. [PubMed: 16571660]  [MGI Ref ID J:109031]

Woods LT; Camden JM; Batek JM; Petris MJ; Erb L; Weisman GA. 2012. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303(7):C790-801. [PubMed: 22875784]  [MGI Ref ID J:192784]

Zhou R; Tardivel A; Thorens B; Choi I; Tschopp J. 2010. Thioredoxin-interacting protein links oxidative stress to inflammasome activation. Nat Immunol 11(2):136-40. [PubMed: 20023662]  [MGI Ref ID J:158410]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes
Mating System	Homozygote x Homozygote         (Female x Male)   20-JUN-06
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	See control note:	C57BL/6J mice carry a naturally occurring point mutation in P2rx7 resulting in a proline to leucine amino acid substitution at residue 451 (P451L).
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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