Description

Strain Information

Former Names C57BL/6J-Ltahlb382    (Changed: 10-JUN-08 ) C57BL/6J-hlb382/J    (Changed: 10-JUN-08 ) Type Chemically Induced Mutation; Coisogenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype b

Description
For information on hlb382 view the web page on the Mouse Heart, Lung, Blood and Sleep Disorders Center site.

Related Strains

Strains carrying other alleles of Lta

005108	B6.129P2-Ltb/Tnf/Ltatm1Dvk/J
002258	B6.129S2-Ltatm1Dch/J
002257	B6;129S2-Ltatm1Dch/J

View Strains carrying other alleles of Lta     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Lta^hlb382/Lta^hlb382

        C57BL/6J-Lta^hlb382/J

• immune system phenotype
• abnormal immune system organ morphology (MGI Ref ID J:134498)
• absent Peyer's patches (MGI Ref ID J:134498)
  • Peyer's patches are absent in these mice
• absent lymph nodes (MGI Ref ID J:134498)
  • mice lack all peripheral and mesenteric lymph nodes and contain no peyer's patches
• absent marginal zone B cells (MGI Ref ID J:134498)
  • there are no marginal zone B cells found in the spleen
• absent mesenteric lymph nodes (MGI Ref ID J:134498)
  • mice lack all mesenteric lymph nodes
• absent spleen germinal center (MGI Ref ID J:134498)
  • germinal centers are absent from the spleen
• absent spleen marginal zone (MGI Ref ID J:134498)
  • the marginal zone is absent in the spleen along with having poorly defined T cells and B cell zones
• decreased NK cell number (MGI Ref ID J:134498)
  • the number of NK cells found in the spleen is reduced by 62% compared to wild-type mice
• decreased transitional stage B cell number (MGI Ref ID J:134498)
  • the absolute numbers of transitional B cells are moderately reduced
• increased B-1b cell number (MGI Ref ID J:134498)
  • there is a dramatic increase in the absolute numbers of B1b cells found in the peritoneum compared to wild-type
  • the percentage of B1-b cells in the peritoneum is more than 2-fold higher than in wild-type controls
• liver inflammation (MGI Ref ID J:134498)
  • small clusters of lymphocytes are observed in the livers of mice
• lung inflammation (MGI Ref ID J:134498)
  • small clusters of lymphocytes are observed in the lungs of mice
• hematopoietic system phenotype
• absent marginal zone B cells (MGI Ref ID J:134498)
  • there are no marginal zone B cells found in the spleen
• absent spleen germinal center (MGI Ref ID J:134498)
  • germinal centers are absent from the spleen
• absent spleen marginal zone (MGI Ref ID J:134498)
  • the marginal zone is absent in the spleen along with having poorly defined T cells and B cell zones
• decreased NK cell number (MGI Ref ID J:134498)
  • the number of NK cells found in the spleen is reduced by 62% compared to wild-type mice
• decreased transitional stage B cell number (MGI Ref ID J:134498)
  • the absolute numbers of transitional B cells are moderately reduced
• increased B-1b cell number (MGI Ref ID J:134498)
  • there is a dramatic increase in the absolute numbers of B1b cells found in the peritoneum compared to wild-type
  • the percentage of B1-b cells in the peritoneum is more than 2-fold higher than in wild-type controls
• liver/biliary system phenotype
• liver inflammation (MGI Ref ID J:134498)
  • small clusters of lymphocytes are observed in the livers of mice
• respiratory system phenotype
• lung inflammation (MGI Ref ID J:134498)
  • small clusters of lymphocytes are observed in the lungs of mice

Lta^hlb382/?

        C57BL/6J

• immune system phenotype
• increased leukocyte cell number (MGI Ref ID J:82961)
  • increased white blood count on non-fasted whole blood analysis (WBC = 15.39 x 10^3 cells per microliter)
• hematopoietic system phenotype
• increased leukocyte cell number (MGI Ref ID J:82961)
  • increased white blood count on non-fasted whole blood analysis (WBC = 15.39 x 10^3 cells per microliter)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Lta^hlb382 related

Hematological Research
Immunological Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ltahlb382
Allele Name		heart, lung and blood 382
Allele Type		Chemically induced (ENU)
Strain of Origin		C57BL/6J
Gene Symbol and Name		Lta, lymphotoxin A
Chromosome		17
Gene Common Name(s)		LT; LT-[a]; LT-alpha; LT[a]; LTalpha; Ltx; TNF beta; TNF-beta; TNFB; TNFSF1; Tnfb; Tnfsf1b; heart, lung and blood 382; hlb382; lymphotoxin; lymphotoxin alpha; tumor necrosis factor beta;
Molecular Note		This phenotypic mutant was identified in an ENU mutagenesis screen. An insertion of a cytosine occurred between nucleotides 16 and 17 of the second exon. This is predicted to cause a frameshift mutation from amino acid 6 and translation of a theoretical new peptide of 173 amino acids with a premature stop codon in exon 4. Because Ltb and Tnf are found in nearby genomic locations, their sequence and expression were analyzed and found to be normal. Intra-cell flow cytometry failed to detect any Lta expression in activated B-cells.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Lta^hlb382 related

JAX National Heart, Lung and Blood Program for Genomic Applications (PGA) URL: http://pga.jax.org. 2003. Heritable mouse mutants from the JAX NHLBI ENU Mutagenesis Program MGI Direct Data Submission :.  [MGI Ref ID J:82961]

Wang H; Feng J; Qi C; Morse HC rd. 2008. An ENU-induced mutation in the lymphotoxin alpha gene impairs organogenesis of lymphoid tissues in C57BL/6 mice. Biochem Biophys Res Commun 370(3):461-7. [PubMed: 18384745]  [MGI Ref ID J:134498]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.