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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x +/+ sibling (Female x Male) 18-APR-08 Species laboratory mouse Generation N12+2F14 (01-NOV-11)
Generation DefinitionsDonating Investigator Clifford Tabin, Harvard Medical School Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Some recombination occurs in the female germline. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning.Development
A transgenic construct containing cre coding sequence under the control of the paired related homeobox 1 promoter was microinjected into B6SJLF2 donor oocytes. Founder animals were backcrossed to C57BL/6J for 12 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: tools
006084 B6.129P2(Cg)-Foxg1tm1(cre)Skm/J 003771 B6.Cg-Tg(Nes-cre)1Kln/J 009388 B6;129S1-Osr2tm2(cre)Jian/J 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 012719 STOCK Tgfb3tm1(cre)Vk/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J View Facebase: tools (8 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Craniofacial and Palate Defects
Orofacial clefting-specific cre expression
Research Tools
Cre-lox System
Cre Recombinase Expression
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Prrx1-cre)1Cjt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Clifford J Tabin | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Prx-1 Cre; Prx-cre; Prx1-cre; Prx1cre; Prx1Cre; | ||
| Mutation Made By | Malcolm Logan, National Institute for Medical Research | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | early limb bud mesenchyme and in a subset of craniofacial mesenchyme, some female germline expression | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Prrx1, paired related homeobox 1, rat | ||
| Driver Note | Prrx1 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a Prrx1 derived enhancer. This transgene is expressed in the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. A germline Cre recombinase activity was observed in female, but not in male, as partially penetrant trait depending on the particular gene flanked by loxP sites. [MGI Ref ID J:77872] | ||
| Gene Symbol and Name | Tg(Prrx1-cre)1Cjt, transgene insertion 1, Clifford J Tabin | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Prx-1 Cre; Prx-cre; Prx1-cre; Prx1cre; Prx1Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Logan M; Martin JF; Nagy A; Lobe C; Olson EN; Tabin CJ. 2002. Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer. Genesis 33(2):77-80. [PubMed: 12112875] [MGI Ref ID J:77872]
Additional References
Tg(Prrx1-cre)1Cjt relatedAhrens MJ; Li Y; Jiang H; Dudley AT. 2009. Convergent extension movements in growth plate chondrocytes require gpi-anchored cell surface proteins. Development 136(20):3463-74. [PubMed: 19762422] [MGI Ref ID J:153618]
Akiyama H; Chaboissier MC; Martin JF; Schedl A; De Crombrugghe B. 2002. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev 16(21):2813-28. [PubMed: 12414734] [MGI Ref ID J:79879]
Amarilio R; Viukov SV; Sharir A; Eshkar-Oren I; Johnson RS; Zelzer E. 2007. HIF1{alpha} regulation of Sox9 is necessary to maintain differentiation of hypoxic prechondrogenic cells during early skeletogenesis. Development 134(21):3917-28. [PubMed: 17913788] [MGI Ref ID J:126336]
Araldi E; Khatri R; Giaccia AJ; Simon MC; Schipani E. 2011. Lack of HIF-2alpha in limb bud mesenchyme causes a modest and transient delay of endochondral bone development. Nat Med 17(1):25-6; author reply 27-9. [PubMed: 21217667] [MGI Ref ID J:168583]
Bandyopadhyay A; Tsuji K; Cox K; Harfe BD; Rosen V; Tabin CJ. 2006. Genetic Analysis of the Roles of BMP2, BMP4, and BMP7 in Limb Patterning and Skeletogenesis. PLoS Genet 2(12):e216. [PubMed: 17194222] [MGI Ref ID J:118257]
Bangs F; Antonio N; Thongnuek P; Welten M; Davey MG; Briscoe J; Tickle C. 2011. Generation of mice with functional inactivation of talpid3, a gene first identified in chicken. Development 138(15):3261-72. [PubMed: 21750036] [MGI Ref ID J:175541]
Barrott JJ; Cash GM; Smith AP; Barrow JR; Murtaugh LC. 2011. Deletion of mouse Porcn blocks Wnt ligand secretion and reveals an ectodermal etiology of human focal dermal hypoplasia/Goltz syndrome. Proc Natl Acad Sci U S A :. [PubMed: 21768372] [MGI Ref ID J:173672]
Bensoussan-Trigano V; Lallemand Y; Saint Cloment C; Robert B. 2011. Msx1 and Msx2 in limb mesenchyme modulate digit number and identity. Dev Dyn 240(5):1190-202. [PubMed: 21465616] [MGI Ref ID J:171190]
Bhattaram P; Penzo-Mendez A; Sock E; Colmenares C; Kaneko KJ; Vassilev A; Depamphilis ML; Wegner M; Lefebvre V. 2010. Organogenesis relies on SoxC transcription factors for the survival of neural and mesenchymal progenitors. Nat Commun 1:9. [PubMed: 20596238] [MGI Ref ID J:175338]
Bimonte S; De Angelis A; Quagliata L; Giusti F; Tammaro R; Dallai R; Ascenzi MG; Diez-Roux G; Franco B. 2011. Ofd1 is required in limb bud patterning and endochondral bone development. Dev Biol 349(2):179-91. [PubMed: 20920500] [MGI Ref ID J:168038]
Blitz E; Viukov S; Sharir A; Shwartz Y; Galloway JL; Pryce BA; Johnson RL; Tabin CJ; Schweitzer R; Zelzer E. 2009. Bone ridge patterning during musculoskeletal assembly is mediated through SCX regulation of Bmp4 at the tendon-skeleton junction. Dev Cell 17(6):861-73. [PubMed: 20059955] [MGI Ref ID J:156015]
Bruce SJ; Butterfield NC; Metzis V; Town L; McGlinn E; Wicking C. 2010. Inactivation of Patched1 in the mouse limb has novel inhibitory effects on the chondrogenic program. J Biol Chem 285(36):27967-81. [PubMed: 20576618] [MGI Ref ID J:166182]
Butterfield NC; Metzis V; McGlinn E; Bruce SJ; Wainwright BJ; Wicking C. 2009. Patched 1 is a crucial determinant of asymmetry and digit number in the vertebrate limb. Development 136(20):3515-24. [PubMed: 19783740] [MGI Ref ID J:153595]
Calo E; Quintero-Estades JA; Danielian PS; Nedelcu S; Berman SD; Lees JA. 2010. Rb regulates fate choice and lineage commitment in vivo. Nature 466(7310):1110-4. [PubMed: 20686481] [MGI Ref ID J:163313]
Canalis E; Zanotti S; Beamer WG; Economides AN; Smerdel-Ramoya A. 2010. Connective tissue growth factor is required for skeletal development and postnatal skeletal homeostasis in male mice. Endocrinology 151(8):3490-501. [PubMed: 20534727] [MGI Ref ID J:163027]
Choocheep K; Hatano S; Takagi H; Watanabe H; Kimata K; Kongtawelert P; Watanabe H. 2010. Versican facilitates chondrocyte differentiation and regulates joint morphogenesis. J Biol Chem 285(27):21114-25. [PubMed: 20404343] [MGI Ref ID J:165936]
Cobb J; Dierich A; Huss-Garcia Y; Duboule D. 2006. A mouse model for human short-stature syndromes identifies Shox2 as an upstream regulator of Runx2 during long-bone development. Proc Natl Acad Sci U S A 103(12):4511-5. [PubMed: 16537395] [MGI Ref ID J:107668]
Compagni A; Logan M; Klein R; Adams RH. 2003. Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell 5(2):217-30. [PubMed: 12919674] [MGI Ref ID J:110732]
Cui Y; Niziolek PJ; Macdonald BT; Zylstra CR; Alenina N; Robinson DR; Zhong Z; Matthes S; Jacobsen CM; Conlon RA; Brommage R; Liu Q; Mseeh F; Powell DR; Yang QM; Zambrowicz B; Gerrits H; Gossen JA; He X; Bader M; Williams BO; Warman ML; Robling AG. 2011. Lrp5 functions in bone to regulate bone mass. Nat Med 17(6):684-91. [PubMed: 21602802] [MGI Ref ID J:172746]
DeRouen MC; Zhen H; Tan SH; Williams S; Marinkovich MP; Oro AE. 2010. Laminin-511 and integrin beta-1 in hair follicle development and basal cell carcinoma formation. BMC Dev Biol 10:112. [PubMed: 21067603] [MGI Ref ID J:166772]
Demehri S; Liu Z; Lee J; Lin MH; Crosby SD; Roberts CJ; Grigsby PW; Miner JH; Farr AG; Kopan R. 2008. Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP, a sentinel for epidermal integrity. PLoS Biol 6(5):e123. [PubMed: 18507503] [MGI Ref ID J:139386]
Dobrowolski R; Hertig G; Lechner H; Worsdorfer P; Wulf V; Dicke N; Eckert D; Bauer R; Schorle H; Willecke K. 2009. Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice. Hum Mol Genet 18(15):2899-911. [PubMed: 19439426] [MGI Ref ID J:150209]
Dong Y; Jesse AM; Kohn A; Gunnell LM; Honjo T; Zuscik MJ; O'Keefe RJ; Hilton MJ. 2010. RBPjkappa-dependent Notch signaling regulates mesenchymal progenitor cell proliferation and differentiation during skeletal development. Development 137(9):1461-71. [PubMed: 20335360] [MGI Ref ID J:159860]
Dy P; Han Y; Lefebvre V. 2008. Generation of mice harboring a Sox5 conditional null allele. Genesis 46(6):294-9. [PubMed: 18543318] [MGI Ref ID J:137217]
Dy P; Smits P; Silvester A; Penzo-Mendez A; Dumitriu B; Han Y; de la Motte CA; Kingsley DM; Lefebvre V. 2010. Synovial joint morphogenesis requires the chondrogenic action of Sox5 and Sox6 in growth plate and articular cartilage. Dev Biol 341(2):346-59. [PubMed: 20206616] [MGI Ref ID J:160493]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Francis JC; Radtke F; Logan MP. 2005. Notch1 signals through Jagged2 to regulate apoptosis in the apical ectodermal ridge of the developing limb bud. Dev Dyn 234(4):1006-15. [PubMed: 16245338] [MGI Ref ID J:102852]
Fujimori S; Novak H; Weissenbock M; Jussila M; Goncalves A; Zeller R; Galloway J; Thesleff I; Hartmann C. 2010. Wnt/beta-catenin signaling in the dental mesenchyme regulates incisor development by regulating Bmp4. Dev Biol 348(1):97-106. [PubMed: 20883686] [MGI Ref ID J:166947]
Galli A; Robay D; Osterwalder M; Bao X; Benazet JD; Tariq M; Paro R; Mackem S; Zeller R. 2010. Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development. PLoS Genet 6(4):e1000901. [PubMed: 20386744] [MGI Ref ID J:159210]
Gamer LW; Tsuji K; Cox K; Capelo LP; Lowery J; Beppu H; Rosen V. 2011. BMPR-II is dispensable for formation of the limb skeleton. Genesis 49(9):719-24. [PubMed: 21538804] [MGI Ref ID J:175737]
Gao Y; Lan Y; Liu H; Jiang R. 2011. The zinc finger transcription factors Osr1 and Osr2 control synovial joint formation. Dev Biol 352(1):83-91. [PubMed: 21262216] [MGI Ref ID J:171478]
Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903. [PubMed: 16040801] [MGI Ref ID J:100475]
Hasson P; Del Buono J; Logan MP. 2007. Tbx5 is dispensable for forelimb outgrowth. Development 134(1):85-92. [PubMed: 17138667] [MGI Ref ID J:117064]
Haycraft CJ; Zhang Q; Song B; Jackson WS; Detloff PJ; Serra R; Yoder BK. 2007. Intraflagellar transport is essential for endochondral bone formation. Development 134(2):307-16. [PubMed: 17166921] [MGI Ref ID J:117033]
Hill TP; Spater D; Taketo MM; Birchmeier W; Hartmann C. 2005. Canonical Wnt/beta-Catenin Signaling Prevents Osteoblasts from Differentiating into Chondrocytes. Dev Cell 8(5):727-38. [PubMed: 15866163] [MGI Ref ID J:98426]
Hill TP; Taketo MM; Birchmeier W; Hartmann C. 2006. Multiple roles of mesenchymal beta-catenin during murine limb patterning. Development 133(7):1219-29. [PubMed: 16495310] [MGI Ref ID J:144140]
Howard PW; Howard TL; Maurer RA. 2010. Generation of mice with a conditional allele for Ift172. Transgenic Res 19(1):121-6. [PubMed: 19521792] [MGI Ref ID J:156426]
Jagani Z; Mora-Blanco EL; Sansam CG; McKenna ES; Wilson B; Chen D; Klekota J; Tamayo P; Nguyen PT; Tolstorukov M; Park PJ; Cho YJ; Hsiao K; Buonamici S; Pomeroy SL; Mesirov JP; Ruffner H; Bouwmeester T; Luchansky SJ; Murtie J; Kelleher JF; Warmuth M; Sellers WR; Roberts CW; Dorsch M. 2010. Loss of the tumor suppressor Snf5 leads to aberrant activation of the Hedgehog-Gli pathway. Nat Med 16(12):1429-33. [PubMed: 21076395] [MGI Ref ID J:167523]
Johnson ET; Nicola T; Roarty K; Yoder BK; Haycraft CJ; Serra R. 2008. Role for primary cilia in the regulation of mouse ovarian function. Dev Dyn 237(8):2053-60. [PubMed: 18629867] [MGI Ref ID J:138332]
Jones KB; Piombo V; Searby C; Kurriger G; Yang B; Grabellus F; Roughley PJ; Morcuende JA; Buckwalter JA; Capecchi MR; Vortkamp A; Sheffield VC. 2010. A mouse model of osteochondromagenesis from clonal inactivation of Ext1 in chondrocytes. Proc Natl Acad Sci U S A 107(5):2054-9. [PubMed: 20080592] [MGI Ref ID J:157599]
Kesper DA; Didt-Koziel L; Vortkamp A. 2010. Gli2 activator function in preosteoblasts is sufficient to mediate Ihh-dependent osteoblast differentiation, whereas the repressor function of Gli2 is dispensable for endochondral ossification. Dev Dyn 239(6):1818-26. [PubMed: 20503377] [MGI Ref ID J:160585]
Kimura A; Inose H; Yano F; Fujita K; Ikeda T; Sato S; Iwasaki M; Jinno T; Ae K; Fukumoto S; Takeuchi Y; Itoh H; Imamura T; Kawaguchi H; Chung UI; Martin JF; Iseki S; Shinomiya K; Takeda S. 2010. Runx1 and Runx2 cooperate during sternal morphogenesis. Development 137(7):1159-67. [PubMed: 20181744] [MGI Ref ID J:158761]
Kmita M; Tarchini B; Zakany J; Logan M; Tabin CJ; Duboule D. 2005. Early developmental arrest of mammalian limbs lacking HoxA/HoxD gene function. Nature 435(7045):1113-6. [PubMed: 15973411] [MGI Ref ID J:99350]
Kolanczyk M; Kossler N; Kuhnisch J; Lavitas L; Stricker S; Wilkening U; Manjubala I; Fratzl P; Sporle R; Herrmann BG; Parada LF; Kornak U; Mundlos S. 2007. Multiple roles for neurofibromin in skeletal development and growth. Hum Mol Genet 16(8):874-86. [PubMed: 17317783] [MGI Ref ID J:121700]
Kolpakova-Hart E; Jinnin M; Hou B; Fukai N; Olsen BR. 2007. Kinesin-2 controls development and patterning of the vertebrate skeleton by Hedgehog- and Gli3-dependent mechanisms. Dev Biol 309(2):273-84. [PubMed: 17698054] [MGI Ref ID J:124871]
Krawchuk D; Weiner SJ; Chen YT; Lu BC; Costantini F; Behringer RR; Laufer E. 2010. Twist1 activity thresholds define multiple functions in limb development. Dev Biol 347(1):133-46. [PubMed: 20732316] [MGI Ref ID J:165721]
Lehman JM; Laag E; Michaud EJ; Yoder BK. 2009. An essential role for dermal primary cilia in hair follicle morphogenesis. J Invest Dermatol 129(2):438-48. [PubMed: 18987668] [MGI Ref ID J:150229]
Li Y; Qiu Q; Watson SS; Schweitzer R; Johnson RL. 2010. Uncoupling skeletal and connective tissue patterning: conditional deletion in cartilage progenitors reveals cell-autonomous requirements for Lmx1b in dorsal-ventral limb patterning. Development 137(7):1181-8. [PubMed: 20215352] [MGI Ref ID J:158676]
Lin PP; Pandey MK; Jin F; Raymond AK; Akiyama H; Lozano G. 2009. Targeted mutation of p53 and Rb in mesenchymal cells of the limb bud produces sarcomas in mice. Carcinogenesis 30(10):1789-95. [PubMed: 19635748] [MGI Ref ID J:153430]
Lin PP; Pandey MK; Jin F; Xiong S; Deavers M; Parant JM; Lozano G. 2008. EWS-FLI1 induces developmental abnormalities and accelerates sarcoma formation in a transgenic mouse model. Cancer Res 68(21):8968-75. [PubMed: 18974141] [MGI Ref ID J:140636]
Liu W; Selever J; Wang D; Lu MF; Moses KA; Schwartz RJ; Martin JF. 2004. Bmp4 signaling is required for outflow-tract septation and branchial-arch artery remodeling. Proc Natl Acad Sci U S A 101(13):4489-94. [PubMed: 15070745] [MGI Ref ID J:89237]
Mao J; McGlinn E; Huang P; Tabin CJ; McMahon AP. 2009. Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell 16(4):600-6. [PubMed: 19386268] [MGI Ref ID J:149478]
Matsumoto K; Irie F; Mackem S; Yamaguchi Y. 2010. A mouse model of chondrocyte-specific somatic mutation reveals a role for Ext1 loss of heterozygosity in multiple hereditary exostoses. Proc Natl Acad Sci U S A 107(24):10932-7. [PubMed: 20534475] [MGI Ref ID J:161395]
Matsumoto K; Li Y; Jakuba C; Sugiyama Y; Sayo T; Okuno M; Dealy CN; Toole BP; Takeda J; Yamaguchi Y; Kosher RA. 2009. Conditional inactivation of Has2 reveals a crucial role for hyaluronan in skeletal growth, patterning, chondrocyte maturation and joint formation in the developing limb. Development 136(16):2825-35. [PubMed: 19633173] [MGI Ref ID J:152912]
Matsumoto Y; Matsumoto K; Irie F; Fukushi J; Stallcup WB; Yamaguchi Y. 2010. Conditional ablation of the heparan sulfate-synthesizing enzyme Ext1 leads to dysregulation of bone morphogenic protein signaling and severe skeletal defects. J Biol Chem 285(25):19227-34. [PubMed: 20404326] [MGI Ref ID J:164551]
Matsushita T; Chan YY; Kawanami A; Balmes G; Landreth GE; Murakami S. 2009. Extracellular signal-regulated kinase 1 (ERK1) and ERK2 play essential roles in osteoblast differentiation and in supporting osteoclastogenesis. Mol Cell Biol 29(21):5843-57. [PubMed: 19737917] [MGI Ref ID J:153980]
Matsushita T; Wilcox WR; Chan YY; Kawanami A; Bukulmez H; Balmes G; Krejci P; Mekikian PB; Otani K; Yamaura I; Warman ML; Givol D; Murakami S. 2009. FGFR3 promotes synchondrosis closure and fusion of ossification centers through the MAPK pathway. Hum Mol Genet 18(2):227-40. [PubMed: 18923003] [MGI Ref ID J:143273]
McGlinn E; Richman JM; Metzis V; Town L; Butterfield NC; Wainwright BJ; Wicking C. 2008. Expression of the NET family member Zfp503 is regulated by hedgehog and BMP signaling in the limb. Dev Dyn 237(4):1172-82. [PubMed: 18351672] [MGI Ref ID J:132977]
McJunkin K; Mazurek A; Premsrirut PK; Zuber J; Dow LE; Simon J; Stillman B; Lowe SW. 2011. Reversible suppression of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A 108(17):7113-8. [PubMed: 21482754] [MGI Ref ID J:171348]
Minguillon C; Del Buono J; Logan MP. 2005. Tbx5 and Tbx4 are not sufficient to determine limb-specific morphologies but have common roles in initiating limb outgrowth. Dev Cell 8(1):75-84. [PubMed: 15621531] [MGI Ref ID J:95804]
Minguillon C; Gibson-Brown JJ; Logan MP. 2009. Tbx4/5 gene duplication and the origin of vertebrate paired appendages. Proc Natl Acad Sci U S A :. [PubMed: 19995988] [MGI Ref ID J:155811]
Moisan A; Rivera MN; Lotinun S; Akhavanfard S; Coffman EJ; Cook EB; Stoykova S; Mukherjee S; Schoonmaker JA; Burger A; Kim WJ; Kronenberg HM; Baron R; Haber DA; Bardeesy N. 2011. The WTX tumor suppressor regulates mesenchymal progenitor cell fate specification. Dev Cell 20(5):583-96. [PubMed: 21571217] [MGI Ref ID J:173242]
Murakami S; Balmes G; McKinney S; Zhang Z; Givol D; de Crombrugghe B. 2004. Constitutive activation of MEK1 in chondrocytes causes Stat1-independent achondroplasia-like dwarfism and rescues the Fgfr3-deficient mouse phenotype. Genes Dev 18(3):290-305. [PubMed: 14871928] [MGI Ref ID J:88286]
Murchison ND; Price BA; Conner DA; Keene DR; Olson EN; Tabin CJ; Schweitzer R. 2007. Regulation of tendon differentiation by scleraxis distinguishes force-transmitting tendons from muscle-anchoring tendons. Development 134(14):2697-708. [PubMed: 17567668] [MGI Ref ID J:122742]
Naiche LA; Papaioannou VE. 2007. Cre activity causes widespread apoptosis and lethal anemia during embryonic development. Genesis 45(12):768-75. [PubMed: 18064676] [MGI Ref ID J:130492]
Naiche LA; Papaioannou VE. 2007. Tbx4 is not required for hindlimb identity or post-bud hindlimb outgrowth. Development 134(1):93-103. [PubMed: 17164415] [MGI Ref ID J:117423]
Ovchinnikov DA; Selever J; Wang Y; Chen YT; Mishina Y; Martin JF; Behringer RR. 2006. BMP receptor type IA in limb bud mesenchyme regulates distal outgrowth and patterning. Dev Biol 295(1):103-15. [PubMed: 16630606] [MGI Ref ID J:144308]
Pan Y; Liu Z; Shen J; Kopan R. 2005. Notch1 and 2 cooperate in limb ectoderm to receive an early Jagged2 signal regulating interdigital apoptosis. Dev Biol 286(2):472-82. [PubMed: 16169548] [MGI Ref ID J:103603]
Provot S; Zinyk D; Gunes Y; Kathri R; Le Q; Kronenberg HM; Johnson RS; Longaker MT; Giaccia AJ; Schipani E. 2007. Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development. J Cell Biol 177(3):451-64. [PubMed: 17470636] [MGI Ref ID J:134727]
Pryce BA; Watson SS; Murchison ND; Staverosky JA; Dunker N; Schweitzer R. 2009. Recruitment and maintenance of tendon progenitors by TGF{beta} signaling are essential for tendon formation. Development 136(8):1351-61. [PubMed: 19304887] [MGI Ref ID J:147280]
Raducanu A; Hunziker EB; Drosse I; Aszodi A. 2009. Beta1 integrin deficiency results in multiple abnormalities of the knee joint. J Biol Chem 284(35):23780-92. [PubMed: 19586917] [MGI Ref ID J:153435]
Rallis C; Bruneau BG; Del Buono J; Seidman CE; Seidman JG; Nissim S; Tabin CJ; Logan MP. 2003. Tbx5 is required for forelimb bud formation and continued outgrowth. Development 130(12):2741-51. [PubMed: 12736217] [MGI Ref ID J:83258]
Rock JR; Cecilia Lopez M; Baker HV; Harfe BD. 2007. Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Gene Expr Patterns 8(1):19-26. [PubMed: 17911046] [MGI Ref ID J:127128]
Schmidt K; Hughes C; Chudek JA; Goodyear SR; Aspden RM; Talbot R; Gundersen TE; Blomhoff R; Henderson C; Wolf CR; Tickle C. 2009. Cholesterol metabolism: the main pathway acting downstream of cytochrome P450 oxidoreductase in skeletal development of the limb. Mol Cell Biol 29(10):2716-29. [PubMed: 19273610] [MGI Ref ID J:148989]
Selever J; Liu W; Lu MF; Behringer RR; Martin JF. 2004. Bmp4 in limb bud mesoderm regulates digit pattern by controlling AER development. Dev Biol 276(2):268-79. [PubMed: 15581864] [MGI Ref ID J:128571]
Seo HS; Serra R. 2007. Deletion of Tgfbr2 in Prx1-cre expressing mesenchyme results in defects in development of the long bones and joints. Dev Biol 310(2):304-16. [PubMed: 17822689] [MGI Ref ID J:128010]
Seo HS; Serra R. 2009. Tgfbr2 is required for development of the skull vault. Dev Biol 334(2):481-90. [PubMed: 19699732] [MGI Ref ID J:153638]
Song H; Mak KK; Topol L; Yun K; Hu J; Garrett L; Chen Y; Park O; Chang J; Simpson RM; Wang CY; Gao B; Jiang J; Yang Y. 2010. Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression. Proc Natl Acad Sci U S A :. [PubMed: 20080598] [MGI Ref ID J:156541]
Soshnikova N; Montavon T; Leleu M; Galjart N; Duboule D. 2010. Functional analysis of CTCF during mammalian limb development. Dev Cell 19(6):819-30. [PubMed: 21145498] [MGI Ref ID J:169050]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous transgenic mice are bred to wildtype siblings. Homozygous mice, reportedly, are viable and fertile. Mating System Hemizygote x +/+ sibling (Female x Male) 18-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Prrx1-cre)1Cjt
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Prrx1-cre)1Cjt x Noncarrier for Tg(Prrx1-cre)1Cjt $321.00 Noncarrier for Tg(Prrx1-cre)1Cjt x Hemizygous for Tg(Prrx1-cre)1Cjt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Prrx1-cre)1Cjt
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Prrx1-cre)1Cjt x Noncarrier for Tg(Prrx1-cre)1Cjt $417.30 Noncarrier for Tg(Prrx1-cre)1Cjt x Hemizygous for Tg(Prrx1-cre)1Cjt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.